WHITE CELLS AND PLATELETS IN BLOOD TRANSFUSION DEVELOPMENTS IN HEMATOLOGY AND IMMUNOLOGY Lijnen, H.R., Collen, D. and Verstraete, M., eds: Synthetic Substrates in Clinical Blood Coagulation Assays. 1980. ISBN 90-247-2409-0 Smit Sibinga, C.Th., Das, P.C. and Forfar, 1.0., eds: Paediatrics and Blood Transfusion. 1982. ISBN 90-247-2619-0 Fabris, N., ed: Immunology and Ageing. 1982. ISBN 90-247-2640-9 Homstra, G.: Dietary Fats, Prostanoids and Arterial Thrombosis. 1982. ISBN 90-247-2667-0 Smit Sibinga, C.Th., Das, P.C. and Loghem, van 1.1., eds: Blood Transfusion and Prob lems of Bleeding. 1982. ISBN 90-247-3058-9 Dormandy, J., ed: Red Cell Deformability and Filterability. 1983. ISBN 0-89838-578-4 Smit Sibinga, C.Th., Das, P.C. and Taswell, H.F., eds: Quality Assurance in Blood Ban king and Its Clinical Impact. 1984. ISBN 0-89838-618-7 Besselaar, A.M.H.P. van den, Gralnick, H.R. and Lewis, S.M., eds: Thromboplastin Cali bration and Oral Anticoagulant Control. 1984. ISBN 0-89838-637-3 Fondu, P. and Thijs, 0., eds: Haemostatic Failure in Liver Disease. 1984. ISBN 0-89838-640-3 Smit Sibinga, C.Th., Das, P.C. and Opelz, G., eds: Transplantation and Blood Transfusi on. 1984. ISBN 0-89838-686-1 Schmid-Schonbein, H., Wurzinger, L.J. and Zimmerman, R.E., eds: Enzyme Activation in Blood-Perfused Artificial Organs. 1985. ISBN 0-89838-704-3 Dormandy, J., ed: Blood Filtration and Blood Cell Deformability. 1985. ISBN 0-89838-714-0 Smit Sibinga, C.Th., Das, P.C. and Seidl, S., eds: Plasma Fractionation and Blood Trans fusion. 1985. ISBN 0-89838-761-2 Dawids, S. and Bantjes, A., eds: Blood Compatible Materials and their Testing. 1986. ISBN 0-89838-813-9 Smit Sibinga, C.Th., Das, P.C. and Greenwalt, T.J., eds: Future Developments in Blood Banking. 1986. ISBN 0-89838-824-4 Berlin, A., Dean, J., Draper, M.H., Smith, E.M.B. and Spreafico, F., eds: Immuno toxicology. 1987. ISBN 0-89838-843-0 Ottenhoff, T. and De Vries, R.: Recognition of M. leprae antigens. 1987. ISBN 0-89838-887-2 Smit Sibinga, C. Th., Das, P .C. and Engelfriet, C.P., eds: White cells and platelets in blood transfusion. 1987. ISBN 0-89838-976-3 White cells and platelets in blood transfusion Proceedings of the Eleventh Annual Symposium on Blood Transfusion, Groningen 1986, organized by the Red Cross Blood Bank Groningen-Drenthe edited by C.Th. SMIT SIBINGA and P.C. DAS Red Cross Blood Bank Groningen-Drenthe The Netherlands C.P. ENGELFRIET Central Laboratory of the Netherlands Red Cross Amsterdam, The Netherlands 1987 MARTINUS NIJHOFF PUBLISHING a member of the KLUWER ACADEMIC PUBLISHERS GROUP BOSTON / DORDRECHT / LANCASTER Distributors for the United States and Canada: Kluwer Academic Publishers, P.O. Box 358, Accord Station, Hingham, MA 02018-0358, USA for the UK and Ireland: Kluwer Academic Publishers, MTP Press Limited, Falcon House, Queen Square, Lancaster LAI lRN, UK for all other countries: Kluwer Academic Publishers Group, Distribution Center, P.O. Box 322, 3300 AH Dordrecht, The Netherlands Library of Congress Cataloging in Publication Data Symposium on Blood Transfusion (11th: 1986 : Groningen, Netherlands) ~hite cells and platelets in blood transfdsion. (DeveloPQents in hematology and immunology ; 19) Includes bibliographies. 1. Blood platelets--Transfusion--Congresses. 2. Leucocytes--Congresses. I. Smit Sibinga, C. Th. II. Das, P. C. III. Engelfriet, C. P. IV. Stichting Rode Kruis Bloedbank Groningen/Drente. V. Title. VI. Series. [DNLM: 1. Blood Platelets--congresses. 2. Blood Transfusion--congresses. 3. Leukocytes- congresses. WI DE997VZK v.19 1 WB 356 S989w 1986) ~MI77.S96 1986 615'.39 87-7301 ISBN-13: 978-1-4612-9238-8 e-ISBN-13: 978-1-4613-2089-0 DOl: 10.1007/978-1-4613-2089-0 Copyright © 1987 by Martinus Nijhoff Publishing, Boston Softcover reprint of the hardcover 1st edition 1987 All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, mechanical, photocopying, recording, or otherwise, without the prior written permission of the publishers, Martinus Nijhoff Publishing, 101 Philip Drive, Assinippi Park, Norwell, MA 02061, USA. TRAVENOL Acknowledgement This publication has been made possible through the support of Travenol, which is gratefully acknowledged. VII CONTENTS Moderators & Speakers .................................... XI Foreword ................................................ XIII I. Principles and functional aspects The human MHC (HLA) and immediate relevance in transfusion practice (S.B. Moore) ............................................ . Alloimmunization against platelets and granulocytes (GP' Engelfriet) .......................................... 13 Leukocytes in transfusion medicine - an overview of functions and clinical implications O. Seidel) ................................................ 17 Platelet functions and platelet transfusion O.W. ten Cate) ........................................... 37 Discussion 43 II. Clinical Aspects A. Presence of cells Platelet transfusion (S. Murphy) ............................................. 47 Prospective selection of compatible platelet donors for alloimmunized patients by an ELISA platelet cross-match only (K. Sintnicolaas) .......................................... 53 White blood cell transfusions in the immuno-compromised patients (A.B. Glassman) .......................................... 59 Immunization with antigen-bound antibody: an experimental approach to prevention of sensitization to allogeneic blood cells (P. Terness) .............................................. 69 Autologous bone marrow transplantation in acute myeloblastic leukemia (AML): in vitro studies to detect minimal disease in remission marrow (B. Lowenberg) ........................................... 85 VIn Autologous bone marrow transplantation in solid malignancies (Po.M. Mulder) .......................................... 93 Discussion 101 B. Absence of cells Clinical use of leukocyte poor red cell concentrates 0Th.M. de Wolf) ......................................... 103 Management and prevention of cytomegalovirus infection (T.H. The) .............................................. 109 Graft-versus-Host disease and the role of blood product irradiation (S.F. Leitman) ............................................ 115 Perspectives for lymphokine therapy: from genetic engineering to immunologic engineering (R.T. Schooley) ........................................... 131 Discussion 143 III. Production aspects Practical aspects of platelet concentrate production and storage (S.]. Slichter) ............................................. 147 Surge plateletapheresis (M.K. Elias) ............................................. 163 Platelets and propylene glycol: an approach to freezing platelets in the presence of a new cryoprotectant (F.G. Arnaud) ............................................ 175 Bacterial contamination in platelets stored at ambient temperature (D.H. Buchholz) .......................................... 189 Preparation of leukocyte-poor blood (B. Brozovic) ............................................. 199 Use of hemapheresis-derived hemopoietic stem cells for transplantation in malignant lymphohemopoietic disorders (M. Korbling) ............................................ 207 Purging of bone marrow (M.K. Brenner) .......................................... 225 Discussion 237 IX IV. Laboratory aspects HLA-matching and cross matching in platelet transfusion O.M. Beelen) ............................................. 243 Cell culture and immune function tests O.W. Smit) ............................................... 249 Platelet antibody detection (A.E.G. Kr. von dem Borne) ................................ 261 Decreased fluidity of human platelet membranes after interaction with an anti PIft antibody (P. Champeix) ............................................ 269 Role of white cells in reagents for AIDS (F. Rey) ................................................ . 273 Discussion 281 XI MODERATORS AND SPEAKERS Moderators c.P. Engelfriet - Central Laboratory of the Netherlands Red Cross, (chairman) Amsterdam, NL j.c. Chermann - Institute Pasteur, Paris, F P.c. Das - Red Cross Blood Bank Groningen-Drenthe, Groningen, NL M.R. Halie - Dept. of Internal Medicine, University of Groningen, Groningen, NL M. Korbling - Ruprechts Karls University, Heidelberg, FRG S.B. Moore - Mayo Clinic Blood Bank and Transfusion Service, Rochester, MI, USA S. Murphy - Thomas Jefferson University, Philadelphia, PA, USA Speakers F.G. Arnaud - MRC Medical Cryobiology Group, Cambridge, UK j.M. Beelen - Blood Group Laboratory, University of Groningen, Groningen, NL A.E.G. Kr. von - Central Laboratory of the Netherlands Red Cross, dem Borne Amsterdam, NL M.K. Brenner - The Royal Free Hospital, London, UK B. Brozovic - North London Blood Transfusion Centre, Edgware, UK D.H. Buchholz - Travenol Laboratories, Round Lake, IL, USA j.W. ten Cate - Academic Medical Centre of the University of Amster- dam, Amsterdam, NL M.K. Elias - Red Cross Blood Bank Groningen-Drenthe, Groningen, NL A.B. Glassman - Medical University of South Carolina, Charleston, SC, USA S.F. Leitman - National Institutes of Health, Bethesda, MD, USA XII B. Lowenberg - Dr. Daniel den Hoed Clinic, Rotterdam, NL N.H. Mulder - Dept. of Internal Medicine, University of Groningen, Groningen, NL R.T. Schooley - Harvard Medical School, Boston, MS, USA j. Seidel - Blood Bank, University of Hannover, Hannover, FRG SJ. Slichter - Puget Sound Blood Center, Seattle, WA, USA j.W. Smit - Dept. of Internal Medicine, University of Groningen, NL P. Terness - Ruprecht Karls University, Heidelberg, FRG T.H. The - Dept. of Internal Medicine, University of Groningen, NL j.Th.M. de Wolf - Dept. of Internal Medicine, University of Groningen, NL Prepared discussants C. Kaplan - National Blood Transfusion Service, Paris, F K. Sintnicolaas - Dr. Daniel den Hoed Clinic, Rotterdam, NL