PROC. ENTOMOL. SOC. WASH. 93(1), 1991, pp. 186-192 TWO NEW SPECIES OF MATSUCOCCUS COCKERELL (HOMOPTERA: MARGARODIDAE) SIMILAR TO MATSUCOCCUSALABAMAE MORRISON ' Charles H. Ray, Jr. and Michael L. Williams Department of Entomology, Alabama Agricultural Experiment Station, Auburn Uni- versity,Alabama 36849-5413; (currentaddress, CHR)OfficeofSafetyand Environmental Health, Auburn University, Alabama 36849-5317. Abstract. —The adult females of 2 new species of the margarodid genus Matsucoccus (Homoptera: Coccoidea), M. banksianae and M. oocarpae are described and illustrated. Matsucoccus banksianae was collected on Pinus banksiana in Minnesota. Matsucoccus oocarpae was collected on P. oocarpa in Guatemala. Both species are morphologically similarto M. alabamaeMorrison, M. californicusMorrison andM. vexillorum Morrison, Key Words: Homoptera, Coccoidea, Margarodidae, Matsucoccus banksianae, Matsu- coccus oocarpae The genus Matsucoccus Cockerell (1909) by Ray and Williams (1984) is modified to is a group of pine-infesting margarodids accommodate the new species. In the fol- found throughout most areas ofthe North- lowing descriptions, measurements are giv- em Hemisphere where pines are found. Re- en in microns and are presented in the text centinvestigationshaveledtothediscovery as the average followed in parentheses by of2 new species, increasing the number of the range. Measurements ofM. banksianae described species in the Western Hemi- arebased on a series of 10 specimens, those sphere to 19. Both new species are mor- of M. oocarpae on the 4 available speci- phologically similar to 3 species described mens. by Morrison (1939), Matsucoccus alaba- mae, M. californicus and M. vexillorum. Matsucoccus banksianae^ Matsucoccus alabamae is found in the New Species Southeastern United States, M. californicus (Fig. 1) in the Western United States and M. vex- illorum in the Southwestern United States. Etymology. —This species is named after Includedin this paperarecomprehensive itsonlyknownhost,jackpine,Pinusbanks- descriptions of adult females of two new iana Lambert. species of Matsucoccus, one from Minne- Material studied.—Pinus banksiana: 1, sota and one from Guatemala. The key to Cass Co., Minn., June 13, 1941, R. F. An- theNorthAmericanspeciesoiMatsucoccus derson (Paratype, USNM); 1, Cass Co., Minn. July 10, 1941, R. F. Anderson (Para- type, USNM); 5, Cass Co., Minn. July 13, ' Part ofa dissertation submitted to the Graduate 1941, R. F. Anderson (Paratype, USNM); School ofAuburn University in partial fulfillment of the Ph.D. degree requirements. Alabama Agricultural 1, Cass Co., Minn. July 16, 1941, R. F. Experiment Station Journal Series No. 17-902467P. Anderson (Paratype, USNM); 5(10), Ely, VOLUME 93, NUMBER 1 187 Minn. June 1962, J. C. Bean (Holotype, setae 1 1 (7-14)long; longestnearcoxae(Fig. Paratype, USNM). IE) 12 (9-21). Antennae (Fig. IF) well de- General description. —Body (Fig. lA) veloped, 9-segmented, 627 (521-728) long. parallel-sidedtoelongate-oval, widestpoint Terminal 4 segments with fleshy setae. Seg- on abdomen, posterior apex more broadly mentIIwith4-7campaniformsensilla.Seg- roundedthanhead; 3538 (2252-4814)long, ments III through VIII with 0-2 interseg- 1507 (1 182-1875) wide. Legs and antennae mental sensilla and 0-3 coeloconic sensilla. well developed; fleshy sensory setae on an- Scape with a dorsal cluster of8-13 setae, 7 tennal segments 6 to 9 inclusive; multiloc- (5-10) long; longest seta on segment II 23 ulardisc pores present, body setae of 1 size; (19-31) long; fleshy setae on segment VIII bands ofcicatrices varying widely. 20 (15-23) long. Legs (Fig. IG) well devel- Dorsalsurface. —Six to 10 setae (Fig. IB) oped, 804 (712-981) long; 2 claw digitules scattered on head, 5 rowsencircling thorax, and 2 setiform tarsal digitules. Trochanters 15-30 in row 1, 18-52 in row 2, 32^6 in with 6-1 1 sensoria and usually 1 long seta. row 3, 14-40 in row 4, 33-57 in row 5; 8 First tarsal segment with 1-4 campaniform rows encircling abdomen, 24-39 in row 1, sensilla. Thoracicspiracles(Fig. 1H) typical 22-40 in row 2, 21^0 in row 3, 25-56 in ofgenus, anteriorpair62 (42-90) long from row 4, 17-41 in row 5, 20-39 in row 6, 21- orifice to tracheae, width ofatrium 30 (20- 33 in row 7, 19-32 in row 8. Additional 40),widthofsieve 13(11-15);posteriorpair setaein shortmarginalbandsbetweencom- 55 (47-65) long from orifice to tracheae, plete abdominal rows and between anterior width ofatrium 29 (22-35), width ofsieve abdominal row and metathoracic legs. Ab- 12 (9-15). Seven pairs of abdominal spi- dominal setae 8 (6-9) long. Eyes single-fac- racles (Fig. 11), sclerotizedringsurrounding eted, 22 (19-27) in diameter, with trans- orifices; diameter 13 (10-20). Clusterof30- versely oval surrounding sclerotization 60 68 multilocular disc pores (Fig. 1J) at apex (49-69) wide. Bilocular tubular ducts (Fig. of abdomen, total diameter 10 (9-12), di- IC) moderately to heavily sclerotized on ameter of locule ring 6 (5-7), width oftu- lateral margins, margins straight with oc- bular duct orifice 4 (4-5), 10 (8-11) high. casionally swollen bases, divergent, bases Locule ring with 9-13 loculi. Bilocular tu- unsclerotizedtoheavilysclerotized; 11(10- bular ducts in continuous series with, and 15) total diameter, 4 (4-5) width of duct similar to, dorsal bilocular tubular ducts. orifice, 9 (9-10) high. Five to 10 total on One to 4 small discoidal pores (Fig. 1K), 4 head; in 5 rows encircling thorax, 9-15 in (3-5)indiameter, midventrallybetweenpro- row 1, 7-28 in row 2, 14-23 in row 3, 14- and mesothoracic legs and similarly be- 30 in row 4, 25-37 in row 5; 8 rows encir- tween meso- and metathoracic legs. cling abdomen, 26-35 in row 1, 26-36 in Otherstages. —Otherstages are unknown row 2, 30-40 in row 3, 27-39 in row 4, 27- except the cyst stage, third stage males, and 38 in row 5, 23-35 in row 6, 23-28 in row adult males which are similar to those of 7, 9-24 in row 8; in short marginal bands Matsucoccus alabamae. between complete abdominal row and Type material. —WoXoXyxiG. adult female metathoracic legs. Cicatrices (Fig. ID) in 3, and 9 paratype adult females on 5 slides 4, or 5 transverse rows on abdomen, 85- collected on Pinus banksiana, Ely, Minn. 366 in number, 8 (6-10) in diameter; first June 1962byJ. C. Bean. Oneparatypeadult rowlocatedbetween 2ndand 3rdor3rdand female on 1 slidecollectedon P. banksiana, 4th pairs ofabdominal spiracles. Cass Co., Minn. June 13, 1941, by R. F. Ventral surface. —Body setae indistin- Anderson. One paratype adult female on guishable from, and continuous with, rows slide collected on P. banksiana, Cass Co., of dorsal setae; 7-12 on head; abdominal Minn. July 10, 1941, by R. F. Anderson. 1 188 PROCEEDINGS OFTHE ENTOMOLOGICALSOCIETY OFWASHINGTON Five paratype adult females on 5 slides col- porespresent; bodysetaeof1 size; marginal lectedonP. banksiana, CassCo., Minn. July bandofbiloculartubularductsbetween last 13, 1941, by R. F. Anderson. One paratype thoracic row and first abdominal row ex- adult female on 1 slide collected on P. tending entirely across dorsum; circatrices banksiana, Cass Co., Minn. July 16, 1941, in 4 bands with perhaps a slightly-devel- by R. F. Anderson. The holotype and 2 oped 5th band. paratypes deposited in U.S. National Mu- Dorsal i'wr/^c^. —Approximately 8 setae seum of Natural History, Beltsville, Md.; (Fig. 2B) scattered on head, 5 rows encir- one paratype in Coccoidea collections of cling thorax, 25-26 in row 1, 31-53 in row each ofthe following: University ofGeor- 2, 29-46 in row 3, 23-28 in row 4, 50-5 gia, Experiment; Florida State Collection of in row 5; 8 rows encircling abdomen, 32- Arthropods, Division of Plant Industry, 34 in row 1, 35-43 in row 2, about 36 in Florida Department of Agriculture and row 7, about 37 row 8 (other rows not enu- Consumer Services, Gainesville; California merated due to poor condition of speci- Department ofFood and Agriculture, Sac- mens). Additional setae in short marginal ramento; Virginia Polytechnic Instituteand bands between complete abdominal rows State University, Blacksburg; British Mu- and between anterior abdominal row and seum ofNatural History, London, England; metathoracic legs. Abdominal setae 9 (7- Canadian National Collections, Ottawa, 1 1) long. Eyes single-faceted, 26 (26-27) in Canada; Naturhistorishes Museum, Wien, diameter, with transversely oval surround- Austria; University ofCalifornia at Davis; ingsclerotization70(67-74)wide. Bilocular MuseumNationald'HistoireNaturelle, En- tubular ducts (Fig. 2C) with relatively di- tomologie Generale et Appliquee, Paris, vergent tubules, more so basally, sides of France. One paratype has been retained in tubules lightly to moderately sclerotized, the Coccoidea collection of Auburn Uni- bases swollen, bases lightly sclerotized to versity. unsclerotized;about 12totaldiameter, 5 (4- 5) width of duct orifice, 10 (10-11) high. Matsucoccus oocarpae, New Species Eleven to 13 total on head; in 5 rows en- (Fig. 2) circling thorax, 47-74 in row 1, 38-46 in Etymology. —This species is named for row 2, 25-41 in row 3, 31^4 in row 4, 19- itsonlyknownhost, PinusoocarpaSchiede. 22 in row 5; in 8 rows encircling abdomen, Material studied.—Pinus oocarpa: 2(3), 50-64 in row 1, 51-70 in row 2, about 34 Quezaltenango, Guatemala, July 10, 1944, in row 7, about 31 in row 8 (other rows not E.J. Hambleton(Paratype, 1 USNM, 1 AU); enumerated due to poor condition ofspec- 1, Ostuncalco, Guatemala, July 10, 1944, imens). Additional bilocular tubular ducts E. J. Hambleton (Holotype, USNM). in short marginal bands between complete General description. —Body (Fig. 2A) abdominal rows and between anterior ab- parallel-sidedtoelongate-oval, widestpoint dominal row and metathoracic legs, the lat- on abdomen, posterior apex more broadly ter extending across entire dorsum. Ci- roundedthanhead; 3968 (3366-4418)long, catrices (Fig. 2D) in 4 transverse bands on 1972 (1733-2209) wide. Legs and antennae abdomen, 690-890 in number, 8 (6-10) in well developed; fleshy sensory setae on an- diameter; first band located between 2nd tennal segments 6 to 8 (segment 9 missing and 3rdor3rd pairsofabdominal spiracles. from available material); multilocular disc Ventral surface.—Body setae indistin- Fig. I. Matsucoccusbanksianae, n. sp., adult female. VOLUME NUMBER 189 93, 1 1 . 190 PROCEEDINGSOFTHE ENTOMOLOGICALSOCIETY OFWASHINGTON guishable from, and continuous with, rows included in the dry and slide-mounted ma- ofdorsal setae; about 8 on head; abdominal terial. Both stages are typical of their re- setae 13 (10-14) long; longest near coxae spective stages ofMatsucoccus. — (Fig. 2E) 15 (14-15) long. Antennae (Fig. Type material. Holotype adult female 2F) well developed, apparently 9-segment- collected on Pinus oocarpa, Ostuncalco, ed, no specimens with antennae complete. Guatemala, July 10, 1944, by E. J. Ham- SegmentsVI, VIIandVIIIwithfleshysetae. bleton. Three paratype adult females on 2 Although segment 9 was missing on all slides collected on P. oocarpa, Quezalte- available specimens, other species ofMat- nango, Guatemala, July 10, 1944, by E. J. sucoccus in North America with 9-seg- Hambleton. The holotype and 1 paratype mentedantennaehave4-5 setaeand2 fleshy depositedinU.S. National MuseumofNat- setae on segment 9. Segment II with 3-6 ural History, Beltsville, Md.; one paratype campaniform sensilla. Segments III though had been retained in the Coccoidea collec- VI with 0-2 intersegmental sensilla and 0- tion ofAuburn University. 3 coeloconic sensilla. Scape with a dorsal cluster of 1 1-14 setae, 8 (7-9) long; longest Key seta on segment II 41 (38-43) long; fleshy Ray and Williams (1984) provided a key setae 24 (21-26) long. Legs (Fig. 2G) well to the North American species of Matsu- developed, 1084 (985-1204) long; 2 claw digitules and 2 setiform tarsal digitules. cmoacycusbewhmiocdhifiinecdluadsedfo1l7loswpsectioes.acTchoamtmkoe-y Trochanterswith 10-13 sensoriaand long 1 date the new species here described: seta. First tarsal segment with 2 campani- form sensilla. Thoracic spiracles (Fig. 2H) 11 Marginal band of bilocular tubular ducts typical of genus, anterior pair 80 (65-88) betweenlastthoracicrowandfirstabdom- long from orifice to tracheae, width ofatri- inal row extendingacross abdominal dor- um 32 (32-33), width ofsieve 12 (12-12); sum 12 1r. Marginal band ofbilocular tubular ducts posterior pair 75 (67-85) long from orifice betweenlastthoracicrowandfirstabdom- to tracheae, width of atrium 37 (33-40), inal row not extending across abdominal width of sieve 14 (12-15). Seven pairs of dorsum 12a abdominalspiracles(Fig. 21),sclerotizedring 12. Cicatricesin4bands,somespecimenswith slightlydeveloped 5th band; feedingon P. surrounding orifices; diameter 16 (14-21). oocarpa M. oocarpae, n. sp. Clusterof71-92multiloculardiscpores(Fig. 12'. Cicatrices in 5 or more bands; on P. pon- 2J) at apex ofabdomen, total diameter 1 derosa M. vexillorum Morrison (10-12), diameter of locule ring 6 (6-6), 12a. Tubulesofbiloculartubularductsoftenad- width oftubular duct orifice 4 (4-4), 9 (7- herent;feedingonpinesofsubsectionPon- 12) high. Locule ring with 10-13 loculi. Bi- derosae M. californicus Morrison locular tubular ducts in continuous series 12a'. vTeurbguelnets;onfotbifleoecduilnagrotnubpuilnaersduocftssuobfsteecntidoin- with,andsimilarto,dorsalbiloculartubular Ponderosae 2b 1 ducts. Four small discoidal pores (Fig. 2K), 12b. Cicatrices usually in 4 distinct bands, the 4 (4-4) in diameter, midventrally between anterior about Vi width of other bands; meso- and metathoracic legs. Three to 4 feedingon pines ofsubsectionAustrales . M. alabamaeMorrison similar pores between pro- and mesotho- 12b'. Cicatricesinwidelyvaryingbands; feeding racic legs. on P. banksianaofthesubsection Contor- Otherstages. —First instars and cysts are tae M. banksianae, n. sp. Fig. 2. Matsucoccusoocarpae, n. sp., adult female. VOLUME NUMBER 191 93, 1 : 192 PROCEEDINGS OFTHE ENTOMOLOGICALSOCIETY OFWASHINGTON Discussion Literature Cited Matsucoccus alabamae, M. banksianae, Cockerel], T. D. A. 1909. The Japanese Coccidae. M. californicus, M. oocarpae and M. vex- Canadian Entomologist41: 55-56. illorum form agroupofspecieswhich share McKenzie, H. L. 1943. TheseasonalhistoryofA/a?- sucoccus vexillorum Morrison (Homoptera: Coc- the following characteristics. All 5 species lack fleshy sensory setae on antennal seg- coidea: Margarodidae). Microentomology 8: 42- 52. ment 5, have legs and antennae well devel- Morrison, H. 1939. Descriptions ofnew species of oped, possess multilocular disc pores at the Matsucoccus(Hemiptera: Coccidae). Proceedings apexoftheabdomenandpossessonly 1 size ofthe Entomological Society ofWashington 41 of body setae. Matsucoccus alabamae and 1-20. M. californicus feed on the trunks of their Ray,C. H. andM. L. Williams. 1984. Twonewspe- hosts (personal observations) whileM. vex- cies ofMatsucoccus (Homoptera: Margarodidae) illorum feeds on twigs of its hosts (Mc- fromArizonaand Mexicowithakeytospeciesin North America. Annalsofthe Entomological So- Kenzie 1943). All feed on pines ofthe sec- cietyofAmerica 77: 765-769. tion Pinus, each species feeding on pines of a single subsection ofPinus.