The Scandinavian Journal of Clinical « Laboratory Investigation EDITED BY THE SCANDINAVIAN SOCIETY FOR CLINICAL CHEMISTRY AND CLINICAL PHYSIOLOGY MANAGING EDITOR O. Stokke EDITORS J. Hartiala K. O. Pedersen J. H. Henriksen I. Romslo E. Hultman K. Rootwelt M. H@rder U-H. Stenman K. Liewendahl J. Waldenstr6m EDITORIAL SECRETARIES K. Bjgro T. A. Hagve Scand. J. Clin. Lab. Invest. — Vol. 51 — 1991 BLACKWELL SCIENTIFIC PUBLICATIONS Published for Authorization to photocopy for internal or personal use or the internal or personal use of specific clients is granted by Mcedisinsk Fysiologisk Forenings Forlag, Oslo by Medisinsk Fysiologisk Forenings Forlag for librarics and Blackwell Scientific Publications other users registered with the Copyright Clearance Center Osney Mead, Oxford OX2 OEL (CCC) Transactional Reporting Service, provided that the 25 John Strect, London WCIN 2BL base fee of $05.00.per copy is paid directly to the CCC, 23 Ainslic Place, Edinburgh EH3 6AJ 27 Congress Street, Salem, MA 01970, USA. This consent 3 Cambridge Center, Suite 208, Cambridge, MA 02142, docs not extend to other kinds of copying, such as copying USA for general distribution, for advertising or promotional 54 University Street, Carlton, Victoria 3053, Australia purposes, for creating new collective works or for resale. Special requests should be addressed to the Editor. © 1991 Mcdisinsk Fysiologisk Forenings Forlag, Institute of 0036-5513/91 $05.00. Clinical Biochemistry, Rikshospitalet, N-0027 Oslo 1. Printed and bound in Great Britain by the Devonshire Press, Torquay No. 1 Review of a Scandinavian Thesis Recombinant human erythropoietin: experimental and clinical applications: O. J. Nielsen ... Original Articles The influence of extracellular magnesium on cell damage induced by ATP depletion in human fibroblasts: S. R. Kristensen & M. Horder Oxidized low-density lipoprctein reduces piusma coagulation in vitro: M. Aviram & D. Presser Release of immunoreactive human neutrophil proteinase 4, normally and in peritonitis: E. Lundberg, M. Bergenfeldt & K. Ohlsson ... Kidney volume in type 1 (insulin-dependent) diabetic patients with normal or increased urinary albumin excretion: effect of long-term improved metabolic control: B. Feldt-Rasmussen, L. Hegedtis, E. R. Mathiesen & T. Deckert ... Awareness of hypercalcaemia in a hospital population?: A. Frolich, P. McNair & I. Transbol Glycoprotein glycosyltransferase activities in serum in alcohol-abusing patients and healthy controls: H. Stibler & S. Borg ... Haemodialysis-induced changes in red blood cell size-distribution histograms: P. C. Bartels, P. W. Helleman, & J. B. J. Soons Respiratory frequency and distribution of cardiac output in rats breathing gas with different densities: G. W. Bergg & I. Tyssebotn High-performance liquid chromatographic determination cf creatinine: S. Ekelund & P. Paby ... Significance of adrenoceptor-mediated atrial natriuretic factor release in normal humans: N. Keller Estimation of granulocyte and lymphocyte potassium in normal subjects and in patients treated with diuretics because of cardiovascular disease: a methodological study: T. Melchior, D. Hallam & N. T. Pedersen Acute cardiovascular effects of insulin in hyperglycaemic type I diabetics: P. C. Ggtzsche, H. Kelbek, S. F. Vissing, S. L. Nielsen, O. Munck, N. J. Christensen, J. Lyngsde & E. R. Mathiesen . ee ‘ fs - Atrial natriuretic peptide and renal adaptation to contralateral nephrectomy in healthy man: A-L. Kamper, E. B. Pedersen, S. Strandgaard, N-H. Holstein-Rathlou, P. P. Leyssac, P. Skaarup, L. Hemmingsen, J. Holm & O. Munck Referees List of Referees for 1990 No. 2 Original Articles Renal and intestinal calcium-binding proteins in normal and uraemic rats: M. Staun, N. Egfjord, H. Daugaard & K. Olgaard A new assay for transglutaminase: B. Seiving, P. Stenberg & B. Nilsson Glutathione and glutathione-metabolizing enzymes in the erythrocytes of healthy children and in children with insulin-dependent diabetes mellitus, juvenile rheumatoid arthritis, coeliac disease and acute lymphoblastic leukaemia: M-R. Stahlberg & E. Hietanen The influence of extra-umbilical serotonin, PGE, and PGF,,, on prostanoid in human umbilical arteries: G. Haugen, K. Bjgro & S. Stray-Pedersen Reverse-phase ion-pairing high-performance liquid chromatography of phosphocreatine, creatine and creatinine in equine muscle: M. Dunnett, R. C. Harris & C. E. Orme Screening for haemochromatosis: influence of analytical imprecision, diagnostic limit and prevalence on test validity: P. Wiggers, J. Dalhgj, P. Hyltoft Petersen, O. Blaabjerg & M. Horder Detection of neutrophil granulocytes in peritoneal dialysis effluents by means of the Cytur-test®: §. Antonsen, F. B. Pedersen, P. Wang & The Danish Study Group on Peritonitis in Dialysis (DASPID) Evaluation of a laboratory health examination programme in a Swedish industry (Volvo): G. Rose & C. Bengtsson Effects of acute cardioselective and non-selective beta-adrenergic blockade on left- ventricular volumes and vascular resistance at rest and during exercise: H. Kelbak & J. Godtfredsen Effects of calcitonin gene-related peptide (CGRP) on regional haemodynamics and on selected hepato-splanchnic arteries from the rat: a comparison with VIP and atriopeptin II: M. Bratveit, A. Haugan & K. B. Helle Effects of felodipine on the dog kidney: a lithium clearance study: U. Abildgaard, G. Daugaard, P. P. Leyssac & O. Amtorp Blood and plasma volumes determined by carbon monoxide gas, ”’"Tc-labelled erythrocytes, '25]-albumin and the T 1824 technique: J. K. Thomsen, N. Fogh-Andersen, K. Biilow & A. Devantier Local and central orthostatic sympathetic reflexes in Raynaud’s phenomenon: M. Engelhart &J.K. Kristensen Equilibration of labelled and endogenous bile acids in patients with liver cirrhosis after administration of (24-'C) cholic and chenodeoxycholic acids: G. Hedenborg, G. Jonsson, O. Wisén & A. Norman Diurnal variation of plasma atrial natriuretic peptide in normals and patients with enuresis nocturna: S. Rittig, U. B. Knudsen, J. P. Norgaard, H. Gregersen, E. B. Pedersen & J.C. Djurhuus No. 3 Original Articles The relationship between age and venous plasma concentrations of noradrenaline, catecholamine metabolites, DOPA and neuropeptide Y-like immunoreactivity in normal humansubjects M. K. Hetland, E. Eldrup, P. Bratholm & N. J. Christensen Alpha-2-macroglobulin decreases parallel to albumin and haemoglobin after elective surgery: A. Lasson, R. Berling, J. Géransson & K. Ohlsson Correlation between leukocyte-associated plasma proteins and white cell differential count: M. M. Andersen & 1. J. Christensen A comparison of measurements of lean body mass derived by bioelectrical impedance, skinfold thickness and total body potassium. A study in obese and non-obese normal subjects: G. R. Fulcher, M. Farrer, M. Walker, D. Rodham, B. Clayton & K. G. M. M. Alberti Dietary n-6 fatty acids inhibit the incorporation of dietary n-3 fatty acids in thrombocyte and serum phospholipids in humans: a controlled dietetic study: M. Gronn, C. Gorbitz, E. Christensen, A. Levorsen, L. Ose, T-A. Hagve & B. O. Christophersen Tumour marker CA 125 in patients with digestive tract malignancies: C. Haglund, P. Kuusela, P. Roberts & H.Jalanko Quality control does improve the performance of laboratory practice in primary health care: I. Romslo, M. L. Knutsen & K. Brechan Increased levels of plasma homocysteine are associated with nephropathy, but not severe retinopathy in type 1 diabetes mellitus: B. Hultberg, E. Agardh, A. Andersson, L. Brattstrém, A. Isaksson, B. Israelsson & C-D. Agardh f-Adrenoceptor regulation in insulin-dependent diabetes mellitus: G. Sager, L. Slordal, N-E. Huseby &J. Florholmen Effect of diet therapy on maximum aerobic power in obese, hyperglycaemic men with recently diagnosed type 2 diabetes: E. Vanninen, M. Uusitupa, O. Siitonen, J. Laitinen, E. Lansimies& K. Pyéralé Protein kinase C subtypes in human neutrophils: D. Obel, L. H. Rasmussen & N. O. Christiansen Technical Note Use of calcium-titrated sodium heparinate for ionized calcium determination on plasma and whole blood (heparinized blood sampler B-129 Radiometer): C. Sachs, P. Rabouine, M. Chaneac, C. Kindermans & M. Dechaux are ss i ae sia ee: 303 Letter to the Editor Reliability of the Reflotron system for cholesterol determination: R. Leinberger & P.U. Koller ea oe aa ne rs aes a vas er i fae 307 No. 4 Original Articles Biophysical and biochemical alterations in erythrocyte membranes from chronic alcoholics: H. Stibler, F. Beaugé, A. Leguicher & S. Borg Heparin lowers blood pressure and vascular calcium uptake in hypertensive rats: S. Vasdev, V. Prabhakaran & C. A. Sampson The influence of supine relaxation on blood pressure, heart rate and atrial natriuretic peptide in normal subjects: B. K. Mie, B. T. Skadberg, O. L. Myking, A. Aakvaag & O.-J. Ohm A single plasma sample method for estimation of the glomerular filtration rate in infants and children using iohexol, I: establishment of a body weight-related formula for the distribution volume of iohexol: G. Stake & T. Monclair A single plasma sample method for estimation of the glomerular filtration rate in infants and children using iohexol, II: establishment of the optimal plasma sampling time and a comparison with the ”Tc"-DTPA method: G. Stake, E. Monn, K. Rootwelt & T. Monclair Quantification and phenotyping of serum cholinesterase by enzyme antigen immunoassay: methodological aspects and clinical ere J. Hangaard, M. Whittaker, A. G. R. Loft & B. Norgaard-Pedersen Enhanced urinary excretion of albumin and f, microglobulin in essential hypertension induced by atrial natriuretic peptide: H. Eiskj@r, C. E. Mogensen, A. Schmitz & E. B. Pedersen ... be ee des aes ‘ Effects of insulin on renal haemodynamics and sodium handling in normal subjects: K. Norgaard, T. Jensen, P. Skoitt & B. Thorsteinsson, N. E. Bruun, J. Giese & B. Feldt-Rasmussen Oxygen free radical-induced injury in isolated rat hearts: effects of ibuprofen and BW 75Sc: A. G. Semb & J. Vaage Hormonal changes in severely uncontrolled type 1 (insulin-dependent) diabetes mellitus: M. Vdailimdaki, K. Liewendahl, P. Nikkanen & R. Pelkonen Vasodilation by calcitonin gene-related peptide (CGRP) and by transmural stimulation of the methoxamine-contracted rat hepatic artery after pretreatment with guanethidine: M. Bratveit & K. B. Helle ERRATUM No. 5 Original Articles Modifications induced by gestational diabetes mellitus on cellular properties: L. Mazzanti, R. Staffolani, R. A. Rabini, C. Romanini, A. M. Cugini, G. Benedetti, N. Cester, E. Faloia & R. De Pirro Tetranectin in amniotic fluid, maternal serum and fetal fluids: E. V. S. Hogdall, C. K. H@gdall, I. Clemmensen, B. Norgaard-Pedersen & A. G. R. Loft ... The stability of immunological and biological activity of human thyrotropin in buffer: its temperature-dependent dissociation into subunits during freezing: T. Kashiwai, K. Ichihara, H. Tamaki, Y. Endo, M. Kimura, K. Takeoka, N. Amino & K. Mitai Apolipoprotein E phenotypes, serum lipoproteins and apolipoproteins in angiographically assessed coronary heart disease: P. M. J Stuyt, B. J. Brenninkmeijer, P. N. M. Demacker, J. C. M. Hendriks, P. van Elteren, A. F. H. Stalenhoef& A. van ‘t Laar Influence of dietary fat, cholesterol and energy on serum lipids at vigorous physical exercise: B. Ekstedt, E. Jonsson & O. Johnson Changes in leukocyte subpopulations following total hip replacement surgery. Effects of high doses of corticosteroids: H. E. Hggevold, T. Lyberg & O. Reikeras Clinical assessment of haemoglobin values by general practitioners related to analytical and biological variation: G. Thue, S. Sandberg & P. Fugelli Accuracy of ambulatory blood pressure determination: a comparative study: J. C. Barthélémy, A. Geyssant, C. Auboyer, A. Antoniadis, J. Berruyer, & J. R. Lacours Impaired left-ventricular function in insulin-dependent diabetic patients with increased urinary albumin excretion: H. Kelbewk, T. Jensen, B. Feldt-Rasmussen, N. J. Christensen, E. A. Richter, T. Deckert & S. L. Nielsen Influence of sex and growth hormone deficiency on sweating: K. Main, K. O. Nilsson & N. E. Skakkebek Possible role for a plasma factor in the induction and/or maintenance of the state of leukocyte adhesiveness/aggregation in the peripheral blood. A study of mothers and their newborns: A. Lidor, N. Arber, S. Berliner, A. i G.— M. Aronson, A. Neri, J. Pinkh&a sM. P. David a : Interference by cryoglobulins with white blood cell measurements on Coulter Counter: A. Bremmelgaard & J. Nygard The decrease in osmotic fragility of erythrocytes during supplementation with n-3 fatty acids is a transient phenomenon: 7-A. Hagve, M. Gronn & B. O. Christophersen LETTERS TO THE EDITOR No. 6 Original Articles Hyperglycaemia but not hyperinsulinaemia prevents the secretion of glucagon-like peptide- (7-36 amide) stimulated by fat ingestion: H. Takahashi, H. Manaka, K. Suda, N. Fukase, A. Sekikawa, H. Eguchi, M. Tominaga & H. Sasaki Toxicity of sitosterol to human umbilical vein endothelial cells in vitro: K. M. Boberg, K. S. Pettersen & H. Prydz Clinical usefulness of serum tartrate-resistant acid phosphatase activity determination to evaluate bone turnover: L. Scarnecchia, S. Minisola, M. T. Pacitti, V. Carnevale, E. Romagnoli, R. Rosso & G. F. Mazzuoli ... Effects of an essential fatty acid-supplemented diet on leukotriene B,-induced rat neutrophil functions: H. Gyllenhammar, J. Palmblad & B. Ringertz ... Comparison of different standards used in radio-immunoassay for atrial natriuretic factor (ANF): P. H. Rasmussen, N. E. Bruu&m J . Giese Intracellular free calcium concentration and thromboxane A, formation of vascular smooth muscle cells are influenced by fish oil and n-3 eicosapentaenoic acid: R. Locher, A. Sachinidis, C. Brunner & W. Vetter Distribution and elimination of intravenously injected urinary trypsin inhibitor: B.—M. Jonsson-Berling & K. Ohlsson Clearance of xenon-133 from bone marrow in patients with small-cell lung cancer: L. J. Petersen, L. Friberg, J. Jensen, J. Biilow, M. Hansen & J. Madsen A simple and rapid method for purification of rat haptoglobin for production of antiserum: I. Wassdal, A. H. Myrset& L. Helgeland No. 7 Review of Two Scandinavian Theses Physiology and pathophysiology of GIP: a review: T. Krarup & P-H. Groop ... ORIGINAL ARTICLES Possible roles of vascular intima for generation of coagulant activity in patients undergoing coronary thrombolysis with recombinant tissue-type plasminogen activator. A randomized, placebo-controlled study: S. Munkvad, J. Gram & J. Jespersen Effects of short-term treatment with naproxen on kidney function in insulin-dependent diabetic patients with microalbuminuria: L. Nilsen, O. Djgseland, K. Rootwelt & K. J. Berg Plasmin inhibitory effect of rat tissues: comparison with antiplasmin activity of plasma: T. Padré, C. M. van den Hoogen & J. J. Emeis The independent effect of ketone bodies on forearm glucose metabolism in normal man: M. Walker, G. R. Fulcher, H. Marsiaj, H.Orskov & K. G. M. M. Alberti Radio-immunoassay of endothelin in human plasma: S. S. Sorensen Evaluation of DNA-based diagnosis for haemophilia A: C. Wadelius, M. Blombdck, P. Goonewardena, M. Anvret, M. Lindstedt, K. H. Gustavson & U. Pettersson ... Iron stores in 1433, 30- to 60-year-old Danish males. Evaluation by serum ferritin and haemoglobulin: N. Milman & M. Kirchhof.f. . Calprotectin (the L1 protein) during surgery in patients with rheumatoid arthritis: H. Berner Berntzen, G. K. M. Endresen, M. K. Fagerhol, J. Spiechowicz & P. Mowinckel Technical Notes A comparison between haematological parameters in ‘capillary’ and venous blood samples from hospitalized children aged 3 months to 14 years: L. N. W. Daae, M. Hallerud & S. Halvorsen Respiratory gas exchange during treadmill exercise testing: reproducibility and comparison of different exercise protocols: J. E. Nordrehaug, R. Danielson, L. Stangeland, G. A. Rosland & H. Vik-Mo ... No. 8 Original Articies Adrenergic receptors are a fallible index of adrenergic denervation hypersensitivity: A. Dejgaard, S. B. Liggett, N. J. Christensen, P. E. Cryer & J. Hilsted Experimental latent and acute porphyria in the non-fasted rat; preventive effect of propranolol: N. Schoenfeld, R. Mamet, R. Mevasser & A. Atsmon Is the fluorescein dilaurate test suitable for monitoring the effectiveness of pancreatic enzyme replacement therapy?: L. M. Burrell, E.J.S. Boyd& K.G.Wormsley Characterization of immunoreactive pancreastatin in porcine tissues: T. D. Borglum Jensen, J.J. Holst & J. Fahrenkrug Elevated hyaluronan blood concentrations in severely burned patients: H. Onarheim, R. K. Reed & T. C. Laurent The ['4C]-triolein breath test is not valid as a test of fat absorption: N. T. Pedersen, B. B. Jorgensen & T. Rannem Serum dolichols in different clinical conditions: H. Humaloja, R. P. Roine, K. Salmela, E. Halmesmiaki, J. Jokelainen & M. Salaspuro B-Hexosaminidase in cultured normal and mutant human fibroblasts: an immunohisto- chemical and biochemical investigation: M. E. Elsafi, M. 1. Elbashir, B. Hultberg, A. Isaksson, I. Hagerstrand & U. Stenram Effects of hyperglycaemia on kidney function, atrial natriuretic factor and plasma renin in patients with insulin-dependent diabetes mellitus: P. Skgtt, A. Vaag, O. Hother-Neilsen, P. Andersen, N. E. Bruun,J . Giese, H. Beck-Nielsen & H-H. Parving The clearance of iohexol as a measure of the glomerular filtration rate in children with chronicrenal failure: G. Stake, E. Monn, K. Rootwelt & T. Monclair Biological variation in the catalytic concentration of pancreatic a-amylase and triacylglycerol lipase inserum:J. Huguet & X. Fuentes-Arderiu