CONTENTS OF VOLUME 35 Number 1 Cells in focus Duncan F. Rogers The airway goblet cell Medicine in focus Alfred Ayala, Joanne L. Lomas, Pathological aspects of apoptosis in severe sepsis and Patricia S. Grutkoski and shock? Chun-Shiang Chung Molecules in focus Fumio Arisaka, Shuji Kanamaru, The tail lysozyme complex of bacteriophage T4 Petr Leiman and Michael G. Rossmann Reviews Ramareddy V. Guntaka, Triplex-forming oligonucleotides as modulators of gene Balwantkumar R. Varma and expression Karl T. Weber Kenneth R. Cutroneo and Sense oligonucleotide competition for gene promoter bind- Jen-Fu Chiu ing and activation General articles Bernhard Wehrle-Haller and Actin, microtubules and focal adhesion dynamics during Beat A. Imhof cell migration Susan J. Hemmings and Sucrose feeding effects inhibition of y-glutamyltranspepti- Satchan Takaya dase in the liver of the rat: possible mediation by thyroid hormone F. Javier Arias, Pilar Antolin, Musarmins: three single-chain ribosome-inactivating pro- Carlos de Torre, Begonia Barriuso, tein isoforms from bulbs of Muscari armeniacum L. and Rosario Iglesias, M. Angeles Rojo, Miller J. Miguel Ferreras, Eugenio Benvenuto, Enrique Méndez and Tomas Girbés Ying-yi Xiao, Michael A. Beilstein, Development of a ponasterone A-inducible gene expression Mei-chuan Wang, system for application in cultured skeletal muscle cells Juntipa Purintrapiban and Neil E. Forsberg Aya Kurosawa, Ken-ichi Shinohara, Human neutrophils isolated from peripheral blood contain Fumiaki Watanabe, Ku protein but not DNA-dependent protein kinase Keiko Shimizu-Saito, Osamu Koiwai, Kohtaro Yamamoto and Hirobumi Teraoka Ling-Hui Zhang, De-Pei Liu and Finding regulatory sequences Chih-Chuan Liang Teresa Mulet, Catalina Pico, Blood leptin homeostasis: sex-associated differences in Paula Oliver and Andreu Palou circulating leptin levels in rats are independent of tissue leptin expression Book reviews Number 2 Cells in focus Patricia M. Taylor, Puspa Batten, The cardiac valve interstitial cell Nigel J. Brand, Penny S. Thomas and Magdi H. Yacoub Medicine in focus Uli Schmidt and C. Glenn Begley Cancer diagnosis and microarrays Molecules in focus Lars-Ake Fransson Glypicans Juha-Pekka Himanen Eph receptors and ephrins and Dimitar B. Nikolov Review Bruno Sangro, Maite Herraiz Gene therapy of neoplastic liver diseases and Jesus Prieto Short communication Thomas Koeck and Karl Kremser L-Carnitine alters nitric oxide synthase activity in fibro- blasts depending on the peroxisomal status General articles Sung Joon Ahn and In Kook Park The coenzyme thiamine pyrophosphate inhibits the self- splicing of the group I intron Sung-Hee Park, Jung Han Yoon Park, Involvement of transcription factors in plasma HDL protec- Jung-Sook Kang and Young-Hee Kang tion against TNF-a-induced vascular cell adhesion molecule-1 expression Estrella Nunez-Delicado, Mar Sojo, Anomalous oxidation of MDL 73,404 by _ horseradish Francisco Garcia-Carmona peroxidase and Alvaro Sanchez-Ferrer Pirkko J. Pussinen, Jari Metso, Plasma phospholipid transfer protein-mediated reactions are Ritva Keva, Birgit Hirschmugl, impaired by hypochlorite-modification of high density Wolfgang Sattler, Matti Jauhiainen lipoprotein and Ernst Malle Marek Tchorzewski, Dawid Krokowski, The subcellular distribution of the human ribosomal “‘stalk”’ Wojciech Rzeski, Olaf-Georg Issinger components: Pl, P2 and PO proteins and Nikodem Grankowski Eva UL rbankova, Petr Hanak, Substitutional mutations in the uncoupling protein-specific Eva Skobisova, Michal Ruzi¢cka sequences of mitochondrial uncoupling protein UCP1 lead and Petr Jezek to the reduction of fatty acid-induced H* uniport Justin P. Ludeman, Robert N. Pike, Determination of the P), P, and P4 subsite-specificity of Karen M. Bromfield, Peter J. Duggan, factor Xa Julia Cianci, Bernard Le Bonniec, James C. Whisstock and Stephen P. Bottomley Haipeng Cheng, Qi Gao, Min Jiang, Molecular cloning and characterization of a novel human Yushu Ma, Xiaohua Ni, Lingchen Guo, protein phosphatase, LMW-DSP3 Wei Jin, Gentao Cao, Chaoneng Ji, Kang Ying, Weiwen Xu, Shaohua Gu, Yuhong Ma, Yi Xie and Yumin Mao Areta Pankiewicz, Tomasz Sledzinski, Tissue specific, sex and age—related differences in the Anna Nogalska and Julian Swierczynski 6-phosphogluconate dehydrogenase gene expression Christophe Frippiat, José Remacle Down-regulation and decreased activity of cyclin-depen- and Olivier Toussaint dent kinase 2 in H,O>-induced premature senescence Won-Kyo Jung, Pyo-Jam Park Purification and characterization of a new lectin from the and Se-Kwon Kim hard roe of skipjack tuna, Katsuwonus pelamis Number 3 Cells in focus Stewart Sell The hepatocyte: heterogeneity and plasticity of liver cells Yassine Amrani and Airway smooth muscle: contraction and beyond Reynold A. Panettieri Medicine in focus Peter Hewins and Caroline Savage Anti-neutrophil cytoplasm antibody associated vasculitis Elizabeth J. Soilleux and Transplacental transmission of HIV: a potential role for Nicholas Coleman HIV binding lectins Molecules in focus Paul A. Sharp Ctrl and its role in body copper homeostasis Debbie Trinder and Erica Baker Transferrin receptor 2: a new molecule in iron metabolism Review Paul G. Besant, Eiling Tan and Mammalian protein histidine kinases Paul V. Attwood Short communication Bing-Sheng Liu, Ning Wang, Differential hypersensitivity to DNase I in the regulatory Xiao-Kuan Cheng, Ning-Hua Wu region of human hsp90B gene in heat shock and constitu- and Yu-Fei Shen tive expression General articles Lorenza Tacchini, Daniela Fusar-Poli, Activation of signal transducer and activator of transcrip- Marina Sironi, Alberto Mantovani tion 3 in rat liver after heat shock and reperfusion stress and Aldo Bernelli-Zazzera M.T. Abdel-Aziz, M.F. El-Asmar, Retrovirus-mediated human heme oxygenase-1 (HO-/) Dawlat El-Miligy, Hazem Atta, gene transfer into rat endothelial cells: the effect of HO- Olfat Shaker, Maivel H. Ghattas, 1 inducers on the expression of cytokines Hanan Hosni and Nagwa Kamal Krzysztof Gwozdzinski, Lipopolysaccharide from Proteus mirabilis O29 induces Anna Pieniazek and Wiesaw Kaca changes in red blood cell membrane lipids and proteins Zhiyu Wang and Decreased plasminogen activator inhibitor-1 secretion in Michelle Kurpakus-Wheater hypoxic corneal epithelial cells is associated with increased urokinase plasminogen activator activity Vladimir Varlen Bamm, Oxidation of low-density lipoprotein by hemoglobin—hemi- Vladimir Abraham Tsemakhovich chrome and Nurith Shaklai Mariana Gavazza and Angel Catala Melatonin preserves arachidonic and docosapentaenoic acids during ascorbate-Fe** peroxidation of rat testis microsomes and mitochondria Yuji Okatani, Akihiko Wakatsuki, Acutely administered melatonin restores hepatic mitochon- Russel J. Reiter and Yasuyo Miyahara drial physiology in old mice Achilleas D. Theocharis, Altered content composition and structure of glycosamino- Demitrios H. Vynios, glycans and proteoglycans in gastric carcinoma Nikoletta Papageorgakopoulou, Spyros S. Skandalis and Dimitrios A. Theocharis Fan Wang, Xuan Wang, Yu Liu, Inhibitive effect of zinc ion on fatty acid synthase from Wei-Xi Tian and Hai-Meng Zhou chicken liver Announcement The Prize Funds Rank Number 4 Cells in focus Charles W. Archer The chondrocyte and Philippa Francis-West Medicine in focus Josep M. Argilés, Cancer cachexia: the molecular mechanisms Rodrigo Moore-Carrasco, Gemma Fuster, Silvia Busquets and Francisco J. Lopez-Soriano Molecules in focus Gregorio Gomez and Targeting G protein-coupled A2a adenosine receptors to Michail V. Sitkovsky engineer inflammation in vivo Alfonso Pennelli, Paolo Sacchetta, Effects of glutathione on kinetics and structural properties Cesare Catitti, Fernanda Amicarelli of amphibian BbGSTPI1-1 and Carmine Di Ilio Review Mira Grdisa and Martyn K. White Molecular and biochemical events during differentiation of the HD3 chicken erythroblastic cell line General articles Fumiaki Watanabe, Ken-ichi Shinohara, Involvement of DNA-dependent protein kinase in down- Hirobumi Teraoka, Kenshi Komatsu, regulation of cell cycle progression Kouichi Tatsumi, Fumio Suzuki, Takashi Imai, Masashi Sagara, Hideo Tsuji and Toshiaki Ogiu Christian Wadsack, Birgit Hirschmugl, Scavenger receptor class B, type I on non-malignant and Astrid Hammer, Sanja Levak-Frank, malignant human epithelial cells mediates cholesteryl ester- Karen F. Kozarsky, Wolfgang Sattler uptake from high density lipoproteins and Ernst Malle Fa-Jian Hou, Hong Xu Simultaneous existence of cinnamomin (a type II RIP) and and Wang-Yi Liu small amount of its free A- and B-chain in mature seeds of camphor tree Michele Rechia Fighera, GM1 ganglioside attenuates convulsions and thiobarbituric Juliana Sartori Bonini, acid reactive substances production induced by the intras- Telma Grendene de Oliveira, triatal injection of methylmalonic acid Roberto Frussa-Filho, Joao Batista Teixeira Rocha, Carlos Severo Dutra-Filho, Maribel Antonello Rubin and Carlos Fernando Mello Masanori Matsuishi, Gun Saito, Purification and some properties of cathepsin H from Akihiro Okitani and Hiromichi Kato rabbit skeletal muscle Beatrice Arezzini, Benedetta Lunghi, Iron overload enhances the development of experimental Giuseppe Lungarella liver cirrhosis in mice and Concetta Gardi Merja Auvinen, Kristiina Jarvinen, Transcriptional regulation of the ornithine decarboxylase Anneli Hotti, Juha Okkeri, gene by c-Myc/Max/Mad network and retinoblastoma pro- Jens Laitinen, Olli A. Janne, tein interacting with c-Myc Philip Coffino, Mathias Bergman, Leif C. Andersson, Kari Alitalo and Erkki Holtta J.P. Mansell and A.J. Bailey Increased metabolism of bone collagen in post-menopausal female osteoporotic femoral heads Alexander D. Dergunov, Aline Hoy, Charge-based heterogeneity of human plasma lipoproteins Elizaveta A. Smirnova, Sophie Visvikis at hypertriglyceridemia: capillary isotachophoresis study and Gerard Siest Number 5 DIRECTED ISSUE: THE PROTEASOME IN THE REGULATION OF CELL FUNCTION Editorials Geoffrey J. Laurent Editorial: Directed issue on proteasomes Didier Attaix and Yves Briand Editorial Medicine in focus Zongmin Li, Marlon Jansen, Neurodegeneration: linking ubiquitin/proteasome pathway Sha-Ron Pierre and Maria impairment with inflammation E. Figueiredo-Pereira Reviews Noboru Mizushima, Role of the Apgl2 conjugation system in mammalian Tamotsu Yoshimori and autophagy Yoshinori Ohsumi Geoffrey M. Goellner Are Huntington’s and polyglutamine-based ataxias protea- and Martin Rechsteiner some storage diseases? Shigeo Murata, Tomoki Chiba CHIP: a quality-control E3 ligase collaborating with mole- and Keiji Tanaka cular chaperones Cezary Wojcik and George Intracellular localization of proteasomes N. DeMartino Simon S. Wing Deubiquitinating enzymes—the importance of driving in reverse along the ubiquitin—proteasome pathway Michael Groll and Robert Huber Substrate access and processing by the 20S proteasome core particle S. Russ Price Increased transcription of ubiquitin-proteasome system com- ponents: molecular responses associated with muscle atrophy Rasmus Hartmann-Petersen, UBA domain containing proteins in fission yeast Colin A.M. Semple, Chris P. Ponting, Klavs B. Hendil and Colin Gordon General articles Lydie Suty, José Lequeu, Allan Lancon, Preferential induction of 20S proteasome subunits during Philippe Etienne, Anne-Sophie Petitot elicitation of plant defense reactions: towards the charac- and Jean-Pierre Blein terization of “‘plant defense proteasomes”’ Graciela Fuertes, Adoracién Villarroya Role of proteasomes in the degradation of short-lived pro- and Erwin Knecht teins in human fibroblasts under various growth conditions Daniel Taillandier, Eveline Aurousseau, Regulation of proteolysis during reloading of the Lydie Combaret, Charles- Yannick unweighted soleus muscle Guezennec and Didier Attaix Lydie Combaret, Daniel Béchet, Down-regulation of genes in the lysosomal and ubiquitin- Agnes Claustre, Daniel Taillandier, proteasome proteolytic pathways in calpain-3-deficient Isabelle Richard and Didier Attaix muscle Mathew R. Hosler, Shuh-Tuan Targeted disruption of specific steps of the ubiquitin— Wang-Su and B.J. Wagner proteasome pathway by oxidation in lens epithelial cells Curtis J. Wray, Joshua M.V. Mammen, Sepsis upregulates the gene expression of multiple ubiqui- Dan D. Hershko and Per-Olof Hasselgren tin ligases in skeletal muscle Vered Maytal-Kivity, Elah Pick, The COP9 signalosome-like complex in S. cerevisiae and Ron Piran, Kay Hofmann and links to other PCI complexes Michael H. Glickman Kenneth J. Rodgers and Assessment of proteasome activity in cell lysates and tissue Roger T. Dean homogenates using peptide substrates Géraldine Carrard, Marc Dieu, Impact of ageing on proteasome structure and function in Martine Raes, Olivier Toussaint human lymphocytes and Bertrand Friguet Simone Merforth, Lothar Kuehn, Alteration of 20S proteasome-subtypes and proteasome Antonia Osmers and Burkhardt Dahlmann activator PA28 in skeletal muscle of rat after induction of diabetes mellitus Sophie Vigouroux, Luc Farout, Increased muscle proteasome activities in rats fed a poly- Stephan Clavel, Yves Briand and unsaturated fatty acid supplemented diet Mariele Briand Number 6 DIRECTED ISSUE: RENIN-ANGIOTENSIN SYSTEMS: STATE OF THE ART Editorials G.jJ. Laurent Editorial Hugh Montgomery, Steve E. Humphries Renin—angiotensin systems: the new frontier and Po Sing Leung Review A.H. Jan Danser Local renin—angiotensin systems: the unanswered questions Molecules in focus David Coates The angiotensin converting enzyme (ACE) Walter G. Thomas and Angiotensin receptors: form and function and distribution Frederick A.O. Mendelsohn Guoyong Yin, Chen Yan and Angiotensin II signaling pathways mediated by tyrosine Bradford C. Berk kinases Duncan J. Campbell The renin—angiotensin and the kallikrein—kinin systems Beril Tom, Andreas Dendorfer Bradykinin, angiotensin-(1—7), and ACE inhibitors: how do and A.H. Jan Danser they interact? Medicine in focus Saraswati Pokharel, Umesh C. Sharma Left ventricular hypertrophy: virtuous intentions, malign and Yigal M. Pinto consequences Reviews Stefan Engeli, Petra Schling, The adipose-tissue renin—angiotensin—aldosterone system: Kerstin Gorzelniak, Michael Boschmann, role in the metabolic syndrome? Jiirgen Janke, Gérard Ailhaud, Michele Teboul, Florence Massiéra and Arya M. Sharma Matthias Barton, Renata Carmona, Obesity-associated activation of angiotensin and endothelin Jana Ortmann, José E. Krieger in the cardiovascular system and Tobias Traupe Po Sing Leung and Mark C. Chappell A local pancreatic renin-angiotensin system: endocrine and exocrine roles Po Sing Leung, Man Lung Fung Renin—angiotensin system in the carotid body and Michael S.C. Tam Alun Jones and David R. Woods Skeletal muscle RAS and exercise performance Ibrahim C. Haznedaroglu and Towards the understanding of the local hematopoietic bone M. Akif Oztirk marrow renin-angiotensin system Yusuke Suzuki, Marta Ruiz-Ortega, Inflammation and angiotensin II Oscar Lorenzo, Monica Ruperez, Vanesa Esteban and Jesus Egido M.J. McKinley, A.L. Albiston, The brain renin—angiotensin system: location and physio- A.M. Allen, M.L. Mathai, C.N. May, logical roles R.M. McAllen, B.J. Oldfield, F.A.O. Mendelsohn and S.Y. Chai Yao Sun and Karl T. Weber RAS and connective tissue in the heart Jop H. van Berlo and Yigal M. Pinto Polymorphisms in the RAS and cardiac function General articles Siu Po Ip, Pui Chun Kwan, Changes of angiotensin-converting enzyme activity in the Carvell H. Williams, Susan Pang, pancreas of chronic hypoxia and acute pancreatitis Nigel M. Hooper and Po Sing Leung Stephan R. Holmer, Heike Bickebdller, Angiotensin converting enzyme gene polymorphism and Christian Hengstenberg, myocardial infarction a large association and linkage study Friederike Rohlmann, Susanne Engel, Hannelore Lowel, Bjorn Mayer, Jeannette Erdmann, Christian Baier, Gernot Klein, Giinter A.J. Riegger and Heribert Schunkert Christiane Pees, Thomas Unger and Effect of angiotensin AT» receptor stimulation on vascular Peter Gohlke cyclic GMP production in normotensive Wistar Kyoto rats Maurice E. Fabiani, David J. Hawkes, Regulation of angiotensin II receptors in the prostate of the Albert G. Frauman, Chris Tikellis, transgenic (mRen-2)27 rat: effect of angiotensin-converting Colin I. Johnston and Jennifer enzyme inhibition L. Wilkinson-Berka Marilyn Mielke, David J. Paterson, The mechanism underlying the positive inotropic effect of A. Elizabeth Sang, George K. Radda angiotensin II in the isolated perfused rabbit heart: a *!P and Kieran Clarke NMR study Balazs Mihalik, Zsuzsanna Gaborik, Endocytosis of the AT,, angiotensin receptor is indepen- Péter Varnai, Adrian J.L. Clark, dent of ubiquitylation of its cytoplasmic serine/threonine- Kevin J. Catt and Laszlo Hunyady rich region Number 7 Editorial G.j. Laurent 1003 Cells in focus Cells in focus Monica Fabbri, Chanel Smart T lymphocytes and Ruggero Pardi Medicine in focus Helen Parfrey, Ravi Mahadeva «,-Antitrypsin deficiency, liver disease and emphysema and David A. Lomas J.J. Manson and D.A. Isenberg Antiphospholipid syndrome Molecules in focus Wen-Jun He and Wang-Yi Liu Cinnamomin: a multifunctional type II ribosome-inactivat- ing protein Karen E. Anderson and Class I phosphoinositide 3-kinases Shaun P. Jackson Review Jane E. Visvader and Geoffrey Transcriptional regulators in mammary gland development J. Lindeman and cancer Short communications Naomi S. Chadderton and Interaction of platelet factor 4 with fibroblast growth factor Sally E. Stringer 2 is stabilised by heparan sulphate B. Kubista, K. Trieb, I. Herbacek Effect of granulocyte-macrophage colony-stimulating factor and M. Micksche on natural-killer cell mediated cytotoxicity M. Angeles Rojo, Lucia Citores, cDNA molecular cloning and seasonal acumulation of an F. Javier Arias, J. Miguel Ferreras, ebulin l-related dimeric lectin of dwarf elder (Sambucus Pilar Jimenez and Tomas Girbés ebulus L.) leaves General articles K. Trieb and H. Blahovec Suramin suppresses growth, alkaline-phosphatase and telo- merase activity of human osteosarcoma cells in vitro Ana M. Terrasa, Margarita H. Guajardo Peroxidation stimulated by lipid hydroperoxides on bovine and Angel Catala retinal pigment epithelium mitochondria. Effect of cellular retinol-binding protein Dhirendra Nandi, Eric S. Lightcap, Purification and inactivation of 3-hydroxyanthranilic acid Yumee Kim Koo, Xingliang Lu, 3,4-dioxygenase from beef liver Jean Quancard and Richard B. Silverman Loredana Mariniello, Carla Esposito, N-terminus end of rat prostate transglutaminase is respon- Ivana Caputo, Angela Sorrentino sible for its catalytic activity and GTP binding and Raffaele Porta José Luis Gomez, Susana Nieto-Ceron, Purification and properties of hydrophilic dimers of Francisco J. Campoy, acetylcholinesterase from mouse erythrocytes Encarnacién Muinoz-Delgado and Cecilio J. Vidal Tomohiko Suzuki, Hideaki Takao, Evidence of met-form myoglobin from Theliostyla albicilla Kazuko Yamanaka, Harumi Gotoh, radular muscle Takahiro Furukohri and Takashi Takagi Mariarosaria Napolitano Activation of protein kinase C by phorbol esters in human and Elena Bravo macrophages reduces the metabolism of modified LDL by down-regulation of scavenger receptor activity Cynthia Wong and Des R. Richardson {8 }-Thalassaemia: emergence of new and improved iron chelators for treatment Number 8 Cells in focus Jennifer E. Morgan and Muscle satellite cells Terence A. Partridge Medicine in focus Youli Hu, Fatih Tanriverdi, Kallmann’s syndrome: molecular pathogenesis Gavin S. MacColl and Pierre-Marc G. Bouloux Pallavi V. Limaye and S. Sivakami Evaluation of the fluidity and functionality of the renal cortical brush border membrane in experimental diabetes in rats Molecules in focus R.K. Andrews, E.E. Gardiner, Glycoprotein Ib—IX—V Y. Shen, J.C. Whisstock and M.C. Berndt Rajiv Jalan, Debbie Shawcross The molecular pathogenesis of hepatic encephalopathy and Nathan Davies