TThheLee piLdeoppteiidoloopgitcaelrSooclieotygical Society ooff JJaapapnan zaEva LepidopteraScience66(314):104-D1e0c9er,nber201S Tlaxonomi cstatus of Pieris(nap in)esis Fruhstorfeg 1909 (Lepidoptera,Pieridae) feruoTADoKoRo 7-5-35Kinuta,Setagaya-kTuo,kyo157-O07J3apan Abstract The androconia of the lectotyp especimen of Pieri snapi nesis Fruhstorfe r1,909, deposite din NHM Londen (BMNH), are examined in order to confirm it staxonomic status ,Pieris nesis i sshown to be from Honshu (Yamato-sLijiguro isn hJiarpaoncehso eaun)d c]ose]y re]ated to Pieri nsapi (Linnaeu sT)h.e synonymy ef R ne.vis and R meiete M6n6trie s1,857, as previeusl yindicate dby Warren (196 1a)nd Tadokoro (20]5 i)s ,rejected. Pieri nsapi japoni S(h'iarOzu .1952 is considered ajunior synonym of Pieri snesis due to the yea rof priori (tsyy nno.v.). Key words Androconia, Ezo-sujiguroshirocho uJ,apan ,Pierisjaponica ,Pieri smelete, Pierisnesis,Pierisnapi- group,Systematics,Taxonomy,Yamato-sajiguroshirochou. Introduction with the Articl e74 of ICZN for the designationo fthe lectotype There are three distin cstpecies of the Pieri snapi-group and paralectotype. in Japan ,Pieri smelete and two napi-related species, one Eitschberger (1983 )also revised the type localit yof from Hokkaido and the other from Honshu. The taxon Pieri snesis from Sapporo/Hokkaido to North Honshu, Pieri s(napi n)esis Fruhstorf e1r9,09 has been treated by only after reviewing both Fruhstorfer(t90 9an)d the type differe nautthors as the napi-related species either from speclmens. Hokkaido (Ezo -sujiguroshirochou) or from Honshu (Yamato -sujiguroshirochou). The confusion has been Materia lasnd methods cSmsaauaurgspkgeiepdnsogtrse bdoyf Ht oYohfeak tmkchoaaneti fiodli -oecistcn ut bFjoerittugywhupes reestonpo sterhhcfiei emrt(reoynlcphe9 oli0 uonf9 cNra)aonHl,dmMi , ttHsyhot ,aenw thsweiidhcn huga.s NstNphHaeeMtc iMumure sna(elBu omMfN RoHf E)( n Ngnvaieatrnpuoei rnner) emsaoinesduns t "HBalaMuNglyHmraeSnecdi Aet c#ont1 icl4v1eoi8ast0Uni ,2ten"hs ie,I v(nlseFetrciitsgt o1ui,tt)ty eotpyfoe Recently T,adokoro (201 5s)uspected that R (nap ine)sis and of might be Pieri smetete from Hokkaido as previously indicate bdy Warren (1961 )W.arren included the taxon nesis in the melete-group inste aofd the napi-group (napi- related species group) based on the form of its e .A androconia. Furthermore, two handwritten labels, ･li t [megamer aand] [and nbm 37 S, R. B.] attached to the ' lectoty psepecimen, indica ttehat the specimen cou]d be zzN..'k' R melete. In fact R, melete from Hoklcaido resembles the t.- ',-. napi-re]ated species from Honshu (Yamato- '' sojiguroshirochou) in wing markings, and only detailed epinqlto"zaqSm- . l 'J.'gepn""''l･ examination of the androconia can reliably separate them , ･ L･1"tsEW/Y!Ej mwoirnpghol ogmiacraklilnyg,s oAfs tThae dloekcotr oo(t2y0 ps1pe5e c)ciomnefnisrmed p etrhfaet cthtely -lt;R, pt2･.eand.ten'k;.opfSR.Si, ' matched the original descript iofo nFruhstorf e(r1909), ,.･.･f, the taxonomic status of Pieri s(nap ine)sis can only be determin eidf the androconia of the lectotyp sepecimen , t･e,iZl.. t lli.t./tl are examined, The lectotyp especimen was selected by Eitsehberg e(r1983 )d,esignate bdy him as the `holotype' from Fruhstorfer' s4 (2 8, 2 ?) syntypes, while a female BMNH:141802 specimen was named as `Allotype', The author be]ieves that the above treatment in Eitschberg e(r1983 c)omplies Fig .1.Lectotyp especimen of Pieri s<nap ine)sis 8 @ NHM, NII-Electronic Library Service TThheLee piLdeoppteirdoloopgitcaelrSooclieotygical Society ooff JJaapapnan faxonomic status of Pieri s(napi)nesls 105 Fig, 2, The napi-related species 8" from Honshu and Hokkaido (Firstbrood). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Fig.3.AndroconiaofPieris(napi)nesis @ NHM. 16 17 18 19 20 NII-Electronic Library Service TThheLee piLdeoppteirdoloopgitcaelrSooclieotygical Society ooff JJaapapnan 106 T.TADOKPRO Hyogo in Japan ,durin gthe perio dfrom middle of March Result sand discussion tsc2Wpoo0ael 0sclSie9smce,a etpnemadntud-e oacmofthfb oT ettahr hke2e a n0o2yan10paei5m-V ra, fef-2lrsoao0thrm e1i dtH2iho (en sk FpGkaeiiuactfiighe2uod s,r)op' .srTfaethr foeKiema ncHmstopuiner ksocehantuw i2a o3w-n a sg.AVun TReaxrh teer( a sneahtnoaedwdrnpo ci f onin)reinso aiFsm i t gehaxurte rern ea a4cps.tihe-odrwen lfart oeidm n tFshpiee clgieuescr tei3on, ttayhn dsep pfe ecitihrmes e bntornoe soofd on androconia of the lectotyp eof R (nqp ine)sis were examined by the [Androconia] author in the presenc eof Dr Takeo Yamauchi, a researcher of the Museum of Nature and Human As Warren (I961 F)L,ijimo r(i201 2a)nd Thdokor oet aL Activitie sT.he androconia were removed from the (201 3i)ndicat Peide,ri smelete can easily be distinguished fOrewin gupperside of all the specimens and observed by from Pieri snapi or napi-related species by the form of optical microscope of 400 magnifications. A P-plat eof the androconia. The androconia in R melete have the the androconia of R (nap ine)sis has been returned to largest scent cell within the Pieri snapi-group, almost NHM for futu rreeference, double the size of the ones in Pieri snapi-related species Honshu (Firsbrtood) Fig. 4. Androconia of the napi-related species from Honshu and Hokkaido (Firstbrood). Hokkaido(Firbsrtoed) NII-Electronic Library Service TThheLee piLdeoppteirdoloopgitcaelrSooclieotygical Society ooff JJaapapnan Taxonomic status ofPieris (napi n)esis 107 E malaiua, "ultmg, S, E, Tibet; 6,800 fee LJul y6. 1913 (? generatie n)R duleine a?, N. Korea (? generation) tr'vt . e =lk Pierisnopi-retstedspecl es fferismetete su ea form aestiva, Fig, S. Androconi eafR tnelete and R napi related spccies, tan.Hokkaido,2"d'generation Fig.6 ,Androconia ofR nesis and other species [Aft Wearrre n<]96Dl. (Fi g5,). The androconia extracted from the lectotype Conflicts: specimen of R (nap ine)sis (Fig 3.) have the typical form The above conclugion is suggesting that: ofnapi-related species rather than ofR metete, 1 .The type locali toyf Pieri snesis was not Sapporo b,ut Furthermore ,detaile dexamination of these androconia inHonshu. somewhere shows that: 1s p. eTcihmee nla miis n pairs ogbeanbelry atlhl eyb rfoiards ,twbhriochod ians diincdait cetashtaet tdbhye 2i.n sTpheec tspe e(dcFimiegn 6.) waosf Pnoite rthie s nleseicst otthyap ts epWeacirmrene,n (1961) Warren (1961 )F,ujimori (2012 )and Tadokoro et aL 3, The labe l[megame rwaas] a misidentification, and the (2013).2. label [andr b.m 37 S. R, B,] was nothing to do with Warren(1961()Fig1.). The neck width is graduall ytapered from the bottom to the top and the shoulder is not angled, which, Acknowledgments according to Nishika i(201O i)n,dicat etshat the specimen i sprobabl yfrom Honshu instea odf Hokkaido (Fig 3s & 4). Specia lthanks to Dr Blanca Huerta ogf NHM (BMNH) in London, who agreed to loa nthe lectotyp sepecimen of [Win gmarkings] Pieri s(nap in)esis to Japa nfor inspectio na,nd to Dr Takeo Yamauchi of the Museum of Nature and Human APsie irni d,nisecs'iast e dbdey sIchrdiobkeo dirno (Fr2u0h1st5o )rt,fhee r w(in1g9 0ma9 rpk)ienrgsfe cotfly AScctiievnictei esU sn,,iIvnesrtsiitt uoyt feo fH yNoagtou rfaolr hanids k iEnndv icoroordnimneatnitonai match the lectotyp sepecimen, and also match both Pieris with NHM and grea thelp during the inspectio nI. also melete and the napi-related species from Honshu, but do express my sincere appreciation to my frien dDr Ulf not match the napi-related species from Hokkaido in its Eitschberg efror his grea tworks. normal form, The wing markings suggest that the lectoty sppeecimen of Pieri snesis is conspecific with the References napi-related species from Honshu (Fig s1 & 2 ). Eitschbergc rU,., 1983. Systematisc hUentersuchunge nam Pieris Conclusion napi-hr.voniae Komplex (s,L )(.Lepidept ePriaer,idae), Herbipoliana 1(1) :i-xxii .1-504; 1(2) :1-601. Judging from the form of androconia and wing markings, Fruhstorfer ,H., 1909. Ncue palaearktis cPhLeeride n(Pier niessis), Pieri snesis is indicat easd the firs tbrood of the Pieris Int .ent. Z. 3 {16) :88. napi-re]ated species "Yamato- sLijiguroshirochou" from Fu.iimor iN,., 2012. Hakkeurin .Nihonsan Monshirochou Zoku. Honshu. This was previousl syuggested by Eitschberger Ikuhousha T,bkyo. (I nJapanese) (1983 )Ad,ditionall yI, determine that Pieri sjaponica Nighikai, M., 20]O. Preliminar cy]assification study of Pieri snapi Shir6zu ,1952 becomes ajunior synonym of Pieri snesis group in Japan based on the merphological characteristics of due to the year of priority. the scent sca]eg. Butteril i(eS s]} :3- ]1 .(I nJapanesc} NNIII-IEl-eEctlreoncictronic Library Service TThhee　 LLeeplpdiopdteorpoltoeglrcaollogical　 SSooclceityety 　ooff　 JJaapapnan 108 T．TADOKPRO Tadokoro．　T．，2015．　Pieris（nap りnesis　Fruhstorfer，1909　may 　be　a ピ種群の ものである． 　　Ta　　dosPkyioneorrnoyi，　mdTae，）　．T　｝．o　’afSdh　oPriin紛gaka’∫w（2a　η4aLn5召d）’硴　：Ml．l　WM冒1a67nn．g6（tI，rn2　iJ0色a1sp3，a．1nP8rei5sm）7ear（yL　esptiuddyo　poft　etrhea， 2第，一ラ化ミナ（春（型発）香で鱗あかるら事香が嚢推を察除さいれたる部．分）は全体的に幅広く 　　　　P（6i4e）r：i3　sn6a−p4i3−．gr up　in　East　Asia（Par−t1），　Butteij7i（Teesinopalpu）s 3り，ラ肩がミナ張のっ形て状いでなはい，．頸部西の海太（2さ01が0）下のに研い究く結に果従でっ はて本徐州々産に広のがヤ Wa πen，　B，　C，　S．，1961，The　androconial 　scales　and　their　bea丘ng　on マ トスジグロ シロチョウの特徴と一致する．なお，西海（2010） 　　the　questio　nof　speciation 　in　the　genus　Pieris〔Lepidopter）a， の研究結果は夏型個体を対象としているが，著者の知見では春 　　Entomo ’．　fidsk，r82；121−148． 型個体の発香鱗において も同様な傾向が見られる （Fig，4）． 一 摘　要 方．田所 （2015＞によると，原記載文に示されたP．（napi）　nesis の斑紋はスジグロ シロチョ ウ若しくは本州産ヤマ トス ジグロ Pieris（napi ）　nesis の分類上の位置づ け （田所輝夫） シロチョ ウに合致し1 正常型の北海道産のナビ種には合致しな Pシieロriチs（nョapiウ）　とnesiしs ては扱，研わ究れ者たにり，よ本っ州て産北の海ヤ道マ産のトスエ ゾジグスロジグシロロ いはTこadとeがke判roっ（20て15い）るが推（Fi論gs　しlた＆北2）海．以道上産のの考ス察ジかグらロ，Pシ．　nロapiチ　neョsiウs チ ョ ウとして扱われたりまちまちで ある．さらにTadokoro ではなく，Eitschberger〔1984）が分類した通り本州産のヤマ ト （（2na0p1i5）＞　nはesiWsaがrr実enは〔1ナ96ビ1）の種発群香で鱗は研な究くの北結海果道〔F産igの．6ス）かジらグロ「Piシerロis スれジまでグロ曖眛シロでチあっョウた以であ下るの点こがとが明示確唆になされっ たた。．それにより，こ チ ョ ウではない か」と推論した．著者はそれらを検証する為に 1，R　napi 　nesis の 基産地は札幌ではなく本州であっ た， （ロよnaンりpi担）ドne名ンsi自sタの然イ史レプ博クで物トあ館タるイ（「プ旧ホ標ロ；本大タ英（イFi博プg物．」1館）（［レ）Eクiにts保トcタh管bイeさrプれge（のて1r9誤い83用る）にとP 2は．Wなaかrrっeたn（1（9F6i1｝gが．6＞検．鏡したR　napi 　nesisはレクトタイプ標本で 解釈で きる）に指定された♂個体で、著者はこの処置により 3．タイプ標本のラベ ル［megamera ］は誤同定，［andr　bm　37　S，　R、 レク トタイプ指定が正しく行われたと考える亅の発香鱗を検鏡 B，］はWarren（1961＞と無関係． す然にトる区タ・別環こイで境とプ標科にき本学しるたは（研F．i究ナg「．兵5所ビ）庫．」種著県にと者立貸スの人ジし要と出グ望自ロさを然れシ受のロたけ博チ．て物著ョP館者．ウ（n：がa兵のpi検発庫）　鏡香n県esし鱗i立sた大はの結簡学レ果単ク自， nなスesiおジs，のグPロiシeノシriニ／ロsapムチonicとョウなα　）Sっhiてをr6示姿zuす，を1消学95名す2はは。記，本今載州後年産Piにのerよナi　snりビesP種iiseをri（sヤ使（n用マapiすト） るのが適当である 発香鱗の形態に以下の特徴が見られた（Fig　3）： 。 1、香嚢のサイズはスジグロ シロ チョ ウのように大きくなく，ナ （Receive　dSeptember　2，2015．Accepted　November　14，　2015） 一 NNI工I工-EEllecetcrotnlrconic　 LLlibrbarryary　 Service