Symposium on Harmful Marine Algae in the U.S. December 4-9, 2000 Symposium Agenda, Abstracts and Participants Marine Biological Laboratory Woods Hole, Massachusetts Symposium Director: Donald M. Anderson Symposium Coordinator: Judy Kleindinst Steering Committee: Don Anderson Woods Hole Oceanographic Institution Dan Baden University of North Carolina, Wilmington Sue Banahan NOAA, National Ocean Service, Silver Spring JoAnn Burkholder North Carolina State University Pat Glibert University of Maryland Center for Environmental Science John Heisler EPA, Oceans and Coastal Protection Division Dennis McGillicuddy Woods Hole Oceanographic Institution Chris Scholin Monterey Bay Aquarium Research Institute Kevin Sellner NOAA, Coastal Ocean Program, Silver Spring Rick Stumpf NOAA, National Ocean Service, Silver Spring Pat Tester NOAA, National Ocean Service, Beaufort Fran VanDolah NOAA, National Ocean Service, Charleston Tracy Villareal The University of Texas at Austin Session Coordinators/Chairs: (Note: Names of Session Chairs are underlined) ECOHAB – Florida: Karen Steidinger, Pat Tester, Fran VanDolah Gulf of Mexico HABs Quay Dortch, Tracy Villareal Pfiesteria – NC, SC, FL JoAnn Burkholder, Jan Landsberg, Alan Lewitus, Wayne Litaker Pfiesteria – DE, MD, VA Pat Glibert, Dave Oldach, Jeff Shields West Coast HABs Rita Horner, Chris Scholin, Vera Trainer Non-regional HABs Kevin Sellner, Tracy Villareal ECOHAB – GOM Don Anderson, Dave Townsend Brown Tides Sue Banahan, Greg Boyer, Cornelia Schlenk Sponsors: U.S. National Office for Marine Biotoxins and Harmful Algae California Sea Grant College Maryland Sea Grant College Monterey Bay Aquarium Research Institute National Institute of Environmental Health Sciences NOAA / Coastal Ocean Program NOAA / Center for Coastal Monitoring and Assessment National Sea Grant Office New York Sea Grant College South Carolina Sea Grant Consortium Virginia Sea Grant College Symposium on Harmful Marine Algae in the U.S. December 4-9, 2000 Marine Biological Laboratory Woods Hole, Massachusetts Symposium Agenda, Abstracts and Participants Symposium on Harmful Marine Algae in the U.S. Program* December 5-9, 2000 Schedule Tues., Dec. 5 Wed., Dec. 6 Thurs., Dec. 7 Fri., Dec. 8 Sat., Dec. 9 8:30 – 8:50 Welcome and Introduction 8:30 – 12:15 ECOHAB – Florida Pfiesteria research West Coast HAB ECOHAB – GOM Brown Tides (NY, – NC, SC & FL research research NJ, DE, MD, TX) 12:15 – 1:30 Lunch break, Lunch break, Lunch break, Lunch break, Lunch break, Swope Dining Hall Swope Dining Hall Swope Dining Hall Swope Dining Hall Swope Dining Hall 1:30 – 5:15 Gulf of Mexico Pfiesteria research Non-regional HAB Technical Plenary discussion regional HAB – DE, MD & VA research workshops/ demos research (TBA) Break Break Break Break Break 6:00 – 7:30 Dinner, Dinner, Dinner, Dinner, Lobster dinner, Swope Dining Hall Swope Dining Hall Swope Dining Hall Swope Dining Hall Swope Dining Hall 7:30 - Discussion Sessions Poster Session (for Social Hour, Poster Session (for Reception, (TBA) Mon. & Tues. Swope upstairs Thurs., Fri. & Sat. Swope Meigs themes) themes) Room Swope upstairs Swope upstairs *NOTE: All science presentations will be held in the Lillie Auditorium, MBL, MBL Street, Woods Hole. Meals will be held in the Swope Dining Hall, MBL. Symposium on Harmful Marine Algae in the U.S. December 4-9, 2000 Marine Biological Laboratory Woods Hole, Massachusetts Program Schedule: Monday, December 4, 2000 3:00 – 6:00 p.m. Registration, Swope Lobby 6:00 – 7:30 p.m. Dinner, Swope Dining Hall 7:00 – 9:30 p.m. Reception, Swope upstairs lobby Tuesday, December 5, 2000 8:30 – 8:50 a.m. Welcome and Introduction Lillie Auditorium Don Anderson, Woods Hole Oceanographic Institution Session I – ECOHAB – Florida (K. Steidinger, F. Van Dolah, P. Tester – Session Coordinators) (P. Tester – Session Chair) 8:50 – 9:10 ECOHAB: Florida overview – the environment Karen Steidinger, John Walsh, and Gary Kirkpatrick 9:10 – 9:30 Roles of endogenous cellular rhythms and life cycle stage recruitment in Gymnodinium breve bloom development Frances M. Van Dolah, Michele Barbier, Tod A. Leighfield, Karen A. Steidinger, Bill Richardson and Peter M. McGuire 9:30 – 9:50 ECOHAB Florida: fate and effects of brevetoxins in selected biota, water, and sediments along the west Florida shelf, USA Jan Landsberg, Pat Tester, Richard Pierce, Damian Shea, Fran Van Dolah, Emilio Sosa, Mike Henry, Jack Fournie, Leanne Flewelling, Sabrina Varnam, and Tod Leighfield 9:50 – 10:10 ECOHAB – Florida: bio-optics and physiology Gary Kirkpatrick, David Millie, Steve Lohrenz, Oscar Schofield, Gary Fahnenstiel, Donald Redalje and Terrance Evens 10:10 – 10:30 BREAK 3 10:30 – 10:50 Hydrography and nutrient characteristics within the ECOHAB: Florida control volume on the west Florida shelf Gabriel A. Vargo, Cynthia A. Heil, John J. Walsh, Kent Fanning, Carmelo R. Tomas, Karen A. Steidinger, Danylle Ault, Merrie Beth Neely, Kristen Lester, and Rachel Merkt 10:50 – 11:10 ECOHAB Florida, physical oceanography Robert H. Weisberg, Ruoying He, William Hemme, Zhenjiang Li, and Huijun Yang 11:10 – 11:30 The role of behavior in Gymnodinium breve bloom formation Daniel Kamykowski, Gerald S. Janowitz, Gang Liu, Edward J. Milligan, and Robert E. Reed 11:30 – 12:00 Coupled numerical models of Florida red tides of Gymnodinium breve John J. Walsh, W. Paul Bissett, Bradley Penta, Dwight A. Dieterle, Robert H. Weisberg, Zhenjiang Li, and Huijun Yang 12:00 – 12:15 Discussion session (if time allows) 12:15 – 1:30 LUNCH BREAK, Swope Dining Hall Lillie Auditorium Session II – Gulf of Mexico Regional HAB Research (T. Villareal, Q. Dortch, Session Coordinators) (Q. Dortch, Session Chair) 1:30 – 1:50 Pseudo-nitzschia spp. in the northern Gulf of Mexico: overview and response to increasing eutrophication Q. Dortch, M.L. Parsons, G.J. Doucette, G. A. Fryxell, A. Maier, A. Thessen and C.L. Powell 1:50 – 2:10 Algicidal bacteria active against Gymnodinium breve: use of molecular techniques to assess changes in microbial communities following the introduction of bacteria Xavier Mayali and Gregory J. Doucette 2:10 – 2:30 Variable brevetoxin production in Gymnodinium breve attributable to growth conditions and strain differences Richard M. Greene, Janis C. Kurtz, Roman S. Stanley, Cynthia A. Chancy, Michael C. Murrell, Fred J. Genthner, John E. Rogers, and Calvin C. Walker 4 2:30 – 2:50 Gymnodinium breve red tides in the Gulf of Mexico: analysis of brevetoxins and metabolites in shellfish R.W. Dickey and S.M. Plakas 2:50 – 3:10 Gymnodinium breve in the western Gulf of Mexico: two years of coastal sampling off the Texas coast Tracy A. Villareal, Mary A. Brainard, and H. Magaña and Lawrence W. McEachron 3:10 – 3:40 The effect of nitrogen source on the growth and toxicity of speices of the genus Prorocentrum Cary L. Burns and Jonathan R. Pennock 3:40 – 4:00 Discussion session (if time allows) 4:00 – 5:00 Informal discussion groups or free time – put up posters 5:00 – 6:00 INFORMAL SOCIAL HOUR, Swope upstairs Lobby 6:00 – 7:30 - DINNER, Swope Dining Hall 7:30 – DISCUSSION SESSIONS (TBA) Wednesday, December 6, 2000 Lillie Auditorium Session III – Pfiesteria research – NC, SC, & FL (J. Burkholder, J. Landsberg, W.Litaker, A. Lewitus – Session Coordinators) (J. Burkholder, Session Chair) 8:30 – 8:50 Pfiesteria, Pfiesteria-like species, and fish health in Florida: an update Jan Landsberg, Karen Steidinger, Susan Cook, Elizabeth Singh, Emilio Sosa, Ann Forstchen, Robin Wood, Parke Rublee, Paula Scott, Jennifer Wolny, and Brian Bendis 8:50 – 9:10 Preliminary characterization of “Cryptoperidiniopsoid” cultures isolated from Florida Steve L. Morton, Tina Mikulski, Elizabeth R. Fairey, Brad Mitchell, Peter D.R. Moeller, Bill Richardson, Karen Steidinger, and John Ramsdell 9:10 – 9:30 Pfiesteria spp. and “Pfiesteria-like organisms” in South Carolina estuaries Alan J. Lewitus, J.M. Burkholder, C. Cary, H.B. Glasgow Jr., K.C. Hayes, A.F. Holland, J.M. Law, and P.A. Rublee 5 9:30 – 9:50 Nutrient enrichment and the toxic Pfiesteria complex: comparative stimulation by swine effluent, poultry manure leachate, human sewage, and other sources J. Burkholder, C. Zheng, H. Glasgow, N. Deamer-Melia and M. Parrow 9:50 – 10:10 Distribution of Pfiesteria species: comparison of results from water and sediment samples across multiple scales, 1998-2000 Parke A. Rublee, Eric F. Schaefer, Coy Allen, Janera Harris, Holly Bowers, Torstein Tengs, and D.W. Oldach 10:10 – 10:30 BREAK 10:30 – 10:50 Pfiesteria field ecology and toxic activity: trends from a decade of intensive study in North Carolina estuaries R. Reed, H. Glasgow, J. Burkholder, N. Deamer-Melia and M. Mallin 10:50 – 11:10 Toxic Pfiesteria promotes acute and chronic lesions in finfish, in controlled experimental trials H. Glasgow, Jr., R. Smolowitz, N. Deamer-Melia and J. Burkholder 11:10 – 11:30 Interactions between Pfiesteria and representative species of commercially valuable shellfish S. Shumway, J. Springer, J. Burkholder and H. Glasgow 11:30 – 11:50 Characterization of a putative toxin produced by Pfiesteria piscicida J.S. Ramsdell, P.D.R. Moeller, E.R. Fairey, A.C. Melo, K.L. Kimm- Brinson, B. Mitchell, S.A. Morton, N. Deamer-Melia , H.B. Glasgow, and J.M. Burkholder 11:50 – 12:15 Discussion session (if time allows) 12:15 – 1:30 LUNCH BREAK, Swope Dining Hall Lillie Auditorium Session IV – Pfiesteria research – DE, MD & VA (P. Glibert, D. Oldach, J. Shields - Session Coordinators) (P. Glibert, Session Chair) 1:30 – 1:45 Approaches to the investigation and interpretation of possible Pfiesteria- related events in Maryland Robert E. Magnien, David M. Goshorn, David W. Oldach, Holly A. Bowers, and Torstein Tengs 1:45 – 2:00 Intensive monitoring for Pfiesteria and related HAB events 6 B. Boicourt, L. Codispoti, M. Roman, V. Holliday, H. MacIntyre, P. Glibert, R. Magnien, and B. Michael 2:00 – 2:15 Assessing temporal and spatial variability in Pfiesteria piscicida distributions using molecular probing techniques Kathryn J. Coyne, David A. Hutchins, Clinton E. Hare and S. Craig Cary 2:15 – 2:30 Functional type (toxicity status) controls Pfiesteria response to nutrients and algal versus fish prey J. Burkholder, H. Glasgow, P. Glibert, A. Lewitus, M. Parrow, C. Zheng, P. Cancellieri and N. Deamer-Melia 2:30 – 2:45 Nitrogen uptake and nutrient relationships in laboratory cultures and field assemblages of Pfiesteria P.M. Glibert, A. Lewitus, J. Burkholder, H. Glasgow, M. Mulholland, and C. Lee 2:45 – 3:00 Potential grazing on Pfiesteria piscicida by microzooplankton in the Pocomoke River Diane K. Stoecker and Daniel E. Gustafson, Jr. 3:00 – 3:20 BREAK 3:20 – 3:35 Trophic relationships of phytoplankton and microzooplankton with Pfiesteria-like heterotrophic dinoflagellates in Pocomoke River and Transquaking/Chicamacomico Rivers, MD, USA Richard V. Lacouture, Jennifer Gronefeld, Ann Marie Hartsig, Stella Sellner and Amy Imirie 3:35 – 3:50 Results of a series of fish bioassays with the toxic dinoflagellate Pfiesteria piscicida Harold G. Marshall, Andrew S. Gordon, David W. Seaborn, Brian Dyer, William M. Dunstan, and A. Michelle Seaborn 3:50 – 4:05 Skin lesions in estuarine fishes: a comparative pathological evaluation of wild and laboratory-exposed fish Wolfgang K. Vogelbein, Kimberly S. Reece, Jeffrey D. Shields, David E. Zwerner, Patrice L. Mason, Larry W. Haas and Vicki Blazer 4:05 – 4:20 Development and testing of molecular diagnostics for Pfiesteria-like organisms in laboratory and environmental samples Kimberly S. Reece, Nancy A. Stokes, Wolfgang K. Vogelbein, Wayne L. Litaker, Jeffrey D. Shields, Larry W. Haas, Patrice L. Mason, Victoria M. Foster and Eugene M. Burreson 7 4:20 – 4:35 Pfiesteria or fungus? Induction of skin ulcers in menhaden with zoospores of Aphanomyces spp. Yasunari Kiryu, Jeffrey D. Shields, Wolfgang K. Vogelbein, David E. Zwerner, Howard Kator, and Vicki S. Blazer 4:35 – 4:50 The ambush predator Pfiesteria piscicida: fad or fallacy Patrick Gillevet, Thomas Nerad, Michael T. Peglar, Greg C. Garman, Stanley Webb, Bonnie Brown, and Charles J. O'Kelly 4:50 – 5:15 Discussion session (if time allows) 6:00 – 7:30 DINNER, Swope Dining Hall 7:30 – POSTER SESSION, Swope upstairs and Meigs Room Thursday, December 7, 2000 Lillie Auditorium Session V – West Coast HAB research (C. Scholin, R.Horner, V. Trainer – Session Coordinators) (C. Scholin, Session Chair) 8:30 – 9:00 HABs-related physical oceanography off the U.S. west coast Barbara M. Hickey 9:00 – 9:30 The challenges of forecasting and managing toxic Pseudo-nitzschia blooms on the U.S. west coast Vera L. Trainer 9:30 – 10:00 Field studies of toxic phytoplankton in central California: 1999-2000 Mary Silver, Susan Coale, Shonna Dovel, Kathi Lefebvre, Greg Doucette, Ron Tjeerdema, and Rikk Kvitek 10:00 – 10:20 BREAK 10:20 – 10:50 Approaches to the detection of domoic acid in marine food webs Gregory J. Doucette 10:50 – 11:20 Trace metals and Pseudo-nitzschia blooms: a possible role for the toxin domoic acid Eden Rue, Maria Maldonado, Ken Bruland and Mark Wells 11:20 – 11:50 Summary Rita A. Horner 11:50 – 12:15 Discussion session (if time allows) 8