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Reproductive disadvantage in an Indian strain of malarial vector Anopheles stephensi Liston (Diptera: Culicidae) on selections with deltamethrin/synergized deltamethrin PDF

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Preview Reproductive disadvantage in an Indian strain of malarial vector Anopheles stephensi Liston (Diptera: Culicidae) on selections with deltamethrin/synergized deltamethrin

WM soa tang Reproductive disadvantage in an Indian strain of malarial vector Anopheles stephensi Liston (Diptera; Culicidae) ‘on selections with deltamethrin/synergized deltamethrin Sarila KUMAR, WK, K, PILTAT Abetenets Tar browlalge af eyeadurtoe Finn aetiie endwesnort mats oe be moment in planet ypleentig wna ved pets: The Iatany sais weve ened ree The writin i Ce retion Tee op Uns) aad email (APR? sie a etc or sat i ang van Delhi Taia oon keto ils ltznedin 3.) owl obiaaing of deans at PUO 6125 CAML al We eal age ant alee il delanetia at Be adult tag Cll), Ube reproductive ices va evalnted ty reams of the fecundity, erly. ce Tachaliigy andthe length of goncmaph eyeloy. The AWK etna ht sod GO TSI: seal drei the gnadinghiw eyes hor rp AaB sinks, Thess ene consid seo fe the ng dn tans rng M15 4139-94 is AnR st vere ote Se sn, The asin elena aera it AaDjoy sin wis the siglvel Ieele of som reitance, Toe eae intisals Ue peels lve soncaion boric Ue lwee of lau wsinzots a be septal ‘sedreutage. This conclaton wae futher eonfoued by 19.48 te W.O% Secreao ix tho hatch of {eg of the AnD) som os me Fat of the pet arn, 8 wets meng ohweetion ofthe tall mpi ein By ac gent In leva the dean wero lal uta lai toes mst i Tre tuted egress skin! sis fain ie lace date Ingest Ue hp undid i la a alin re +e sl ‘epbaat Uh cule ipl the odo sepodasve “neo saul googie pop “ev imnes heemyge and est henorata by eglovering orn nner vaNegvet Srecges ave tn spe Keg ores nicer seca rk! wine rsicanvey_qigement Hub Tengo rly pci gels yo lenge tanagonent of esting nwo asian in he INTRODUCTION fe The ans i Verena” hues five ene auosyudr-bicae dwensee ie imersele ICMR, a hin, Ainpies seen ice othe wus allen, 3007 rreumen apecion wep hor uli naan Aweorinay "0 WHO {2005}, ulin affects soae than 30H lie mpl i ror shranbnthe Avid anda one of he min suas of malty infnuss aad yonag cheer. Tn 1988, abet 20-000 role aml in $3 GUN lca life yea wove Inet do ts malaria int Tea, The tanto failros of thie anoyits axe eiauelly dae <0 Me dearlayrent of rig iarctisie resisbance sesiet vac inscticiden, pong exons tavat 9 siseentb.e tnuine of Snsvets feeqmenty ier in The asennad ili rasan rsiatunt paputions ea ever inert ths rensgencat of aeaitaure (Campatbela ot a WSL an tle reine wearer toile rly en the svuptian eave nese omlations amocined with eslstumee gence Hovis mal ewig, WOT Hayienn ao BAIN) teem eae verse that itd atl nur txtage (WHO, [OS2), Tedign sunin fg, —‘liore con pda Chena of say 302}. le he sietesos Ie boom ote Us Te osha, on See A seid oon, in geneal. the ‘icbleadiplceslvisMervedbaus (DDT), hesarkeo. Tastes gores soul te dR ot oF ont tyelenane CHCA}, alton ard veionvadvin —prptlstions and the aseotant ategns cabbit a Thao vvtes gual Sastre, 22}. The dns it radial eeprom lene Bo ral Ply, ‘rector contel uuirly voice on the sustoice for the 1952; Kons, 1987: Asweatino of wh, 1989, Li i TRACP ke ooomatpon San ae cil, INL, Kona of ag 2089), eling the Thayer veil ils tr Fea (Tal sink Wehensie, 19ST Haulage N23. A Teall been ies tinsel ibe Peston se ros Tome lg ‘roca ‘lng of gris invalral in Ruler rinse ( MeKrovie ad TOA Arnal ety 20022 inti wee, therein Ue ey Tne Fie salsa wil lle regan, (Amon er Hnesers the reise ye lL yh Bitar (HMkge af 0, ZOTPY the eins Mili nis fv sn chyna af estan Hans sal Meche, 18875 ln ly ela ethno he ae idea insta iden agains ites lle saga of Ar. steps Fan ern el sessed ekg ve Willa 1998; HO the Abelian! prpsltion ofa olay stim ff deat Inet rapa t prion Fompard In ile akon et, 193 HOF} ae iced Ruin et iy sta Misadvanlage a ‘ssi ronan Pi Shensis Oho aa Miia slain A ests, Wary fw sling bv leat ierlven Io Inve gle the rb fse Fess it ellerversisnl sis Hho he ownldge of sara in relative Hy Ahaneshneresiant elvis sah ila teving. satel in beating the yor ro rosa. Keving thal fe ine Fala sales ane suid ans atte el pled sal imeuiiheseesl alin sli Aste to sbily Ue safeties of Usnedlin sand sponge sellin resisbanoe ta Hirsute Town eins Tee othe tly dation a gly exten Frcs sich ex, Tei lle Feller rabeai fin selavethvneselaraaainy ol 4 1 MATERIALS AND METILODS LL Mosquite population ‘Tho present s4veebgatiors oupley tho lereec ara seule af in ephect originate fon fl-ollsctd cogexget feole adults fun De:. India. The colony vas cantina ia uucotery wheat ony iusecticce expoeme at 28 = 1, $0% «Set TUL fond a photopessel of UL? 10D (Kiar a at, 2002). Adhite were supplied with soaked desecded dtian Peviosio blood marae wexe. provided 0 female mowqiters tor egg matamtion by keeping reetsinod alse cats iu tho ceaxe. The opps wore cles el nv an etme font ive th WHosenan Filer pape al sells i a in ane ny Filla eit seer ses sale. Larsie ner Fal yi ivi a aeo sei, sowie sani hy, ln pes Hone wer ls a neal Fats and tase tthe hth cage a all regen 2 Chansical Teale rae sear ethin ith «gig ‘HG lane: rt Reel Lely pions PRO Jy a spumgial of Aetianetri wea in thee an sales eters Tota. Te Tala Ue seamethrinressbanos The resis! sine, ea, Insc icingrsedied ayers ae ameths {OARS} neve procul from Worl Hel Cigaicaton, Connie The: gaye yore lial a AV nal ne anal ar usinnny of 3 Bre 1-3. Larval susceptibility and selcetion studies Whe barca Hiss: ws esto oe nly All, instar wrvaniense ith te BHO val fn nuit Lvse WHO, WI). Te Faroe, grips af 25, see tne fv 24 with Vink ettvnic lin te re ie in 249 ral sim aa penal fom Honnset Tel. a wae eke i gl are Chale ore epee ‘alana amy Coveted rig Aslells Goel 1 AMAL, 1925 The de wore subj Iverson ayia the Ui aml Vinny Sele aloe vem rane Ty esp ese ei uf 40) SU) Tree ar 4 I i are jars col ag 24D uf ten aa) Vale ol he sovnainy inert stn iv eon elton, aloes ure cle i wach Iva Te eamcruraivn fingers a algae Wo the [2 Teel. the mvinnd “are ene eared tothe adult. the Tarmac of the nest evi we suc ly oe aumcepiily tee fit seleted vith the eam intone at LC, 1 Thee selection aries were cout for exeecaivo soucusions ard sepaate eval studies perforaod At Adult sweeptbiity und seketioa studies The adult ewcepsilty studies wots cacé cut by the WIG povcodae weing standard HID ite (WIIO. 1981b3. Freshly Hond-fd 3 old femele fdults ere cspeced ia batches 0 0.029% delamethin-iumprspaated papers ant wetelitie wo30 recorded, Foe aleeion stdice, aceon bates of 20-25 fall Blox-fed wated feualee were expceed to dectauthrn on aa to trace 6 acnality inthe sults. ‘The surviving frales tore vse for clei ‘he txt genertan which wae fants expocod t2 the am Sin KIMAIL rat Ici csi protien rs Heart ae pone 1. Straine used tn present sates ne sara sine let Ine rn aioe sa allows TS] AeS line, AnK stoi paral sei Fld called sean ith Ly taal 0.10118 vg V5.2 An lines (CH! AnD sens delannabyin lana sleet sr Ca} AnDgsslsiny Ani sesin. sleet tl ea of Tal lige ar 0 yeveratans. te delririn =tADASIE g/g eae 6. IY Any aline Any shin aera i eae TAD eerie a Lana ay ey ‘an ebrn =ALORT72 og be nian lio =A 1 2) Aud) stiin: delawedlnin — PLU 11:5) Tneal-saleted ain 13 AnD: stain; ATS smaiy select th rmergzed deliauctin at Laval stage fr 20 senctationa, LC, t9 deltamebrio = 6. 916253 mg’ faL, remtance mo ~ 13.6. by AaUlyy ssin: ADD. steain saietad <0 selection presse of synergize deltaethin at lava ange 40 meena, Ly to setumettzia 0.035367 mme/mL: saisunes sitio ~ 28.9. (3) AnD, steons deltanethein ault-slectod Ca} AnD ain; ARS am expel tug gall stage Far 2 genoa LT, te della = 11-4) in, esse rat 28 Ib) Sag, amin Aan’ megane aD, ain abiceil 0 mls frst 20) Tig tm delineate = TTA wing ssh ris =A. 1.6 Reproductive potential studies din of Fk mesa HKG ais, 50 nals nt 50 Foes of oath rain wee eens inthe seperate ger aul Ut cn sual ened ruisimn- Forte ule eens priv ith la ra slier thine days a etmergeme chil wrk the Inagmsing af Ft gnsoinghie opel Ui ras lat aqui neural Tham wae aes blo sme aie sors att Heal wore sunt Pare halla with oe chlorinated worer wore kopt im the egos to vllect the Inlay fomales, Ate the complet of Set, ound of seprsiton, sundvingfeuales were wounted gon ol puri! dbo ecvord bleed mac ator 2A W of stavetion. The peri fom the boring of ‘one blows! imo tu that af the oext blo! woul recorded as one gonotephae eyele (Kiar os cy 0065. Kubets of blooded females were tered Tans resin got allow ny by egg “The xn nal theo pial il shelved vel alle Ue Smletion of sean ud of sion atl the tne vais tne repeat ae halon thie An jhe We evi ual hil af he sli sous mend suring tho: erelive gamle {giles nas thie prc iow Fines Rar iy tls Ty a hep in ach ey oe gay bl sees allel aul ronal wreey wae aller ic Nl sweat using isting wiemsape atl srg s a 16 iu ale ag er a Firat leat tk Tie ily rvsnls wr hee Hoe ssh gen hi oy ly esi fale per ewe im cand art le Huai ‘Ai sei was cams il the Fei A sisi. The freuen cing thine "pal yah span The eye rollevi laring such gnmiragt gale nme hanged nt ts fehl wad allan i hale septs Fillet ih We hutshe leesan ond the per cent adh ene calls fn nuh fost hy taking rata the tule halle eshte runt apy We per rent Hal An in as eneae ih the jr sen fash of AB is The el aration fi prison in eae rel [8 Data states and analysis The rele abi were drag spe wae ale soe ssn Salon's est itl sainral gna ‘auld far 2851 05 using te eater SPSS Mogan, 2 RESULTS 2.1 Lareaacult-muscepeii sues The Alas Ast ses hl Mo bidet Trove eae 180. 1-690 bel er Ay ftusn {28.994 The Auli, ence exhibited veny low levels of resistanco we dsltonesirin (3.8 136. [+ fold) in compsnsen to the laral-sclected sts 2.2, Duration uf guaviruphic eyeles hur investigations ou the vepd tive Potential of on, staphenct reveled ther tho Ani mame ‘xbihited a eiuificantredartioa of 604 — 3% CE 20.05; 0, O01 to 0. 064 in the danation of 3 cousventsve gynctophic eyelee on ound to the ‘An8 stain (Table 1. The AaD, staine with Incest teeistunoe we celtumethnia exhibited the shortest doveson of genotopiie czcloe <2 compated t9 other and aeeston ua TRACP ke ooomatpon San ae rexitant x tine, thigh the lesan the Aven yu signin (20.08) Develo he saan dh gual yb eyele seat pil tl ye Blea rei Ue aavrersve Hl nel er elle rl aller 24 Wecatvaion pio, ii Apher spore 2.3 Repovinetive potential smaion 2.3.1. Kr gle vt, The parson ‘deals haat! guile! nalution In Ferdi f Ant sein hing he Ha gd cele (Table 312 he nent det aad siguifeant fleet var leeed in tho AnD etaiae wth 16.58% CP 0.02) aud and, emia with (1, 66% reskwed reankts (PO, O43. The sugges tat 2,9-fld iueroseed lovee of dalnnactin resitan. ‘fi 20,6-fod 1» 6D, L-uldl) sevnliod in Avvo Taney iv the AnD) steine af Ae lngiena, Tn xevengens sekonvthrin_ sil all Sahat Arie stains te bn waa in Pasiy (Tile 2) ae 10 Nhe rena Ua fauging tren 8. 45) = 105% enfant (> 0.05, Tesitanre to dakametvin alse Meceoqicl at the Tava) stp xeelted in anio reproductive disadvantae as compared to that developed at adult stage ur developed after larval sults wide saga daltouctra, ake 2 ste of datacom and seed darn slush Rena atcanuly a Avorekes ‘tee! be at pautropte ele ne salen av oe Ieee sang the tot annem «le anid te fet ta fe eae sll the Aa elesion wee spice love Cae ( SO fai! agen ger lene} am eign be patent tain et atch! og (2° 0.08; (ale 22 Tega Buy al Seay et shore le 1G. TSG hatevng ne aginst SO etching slesin (PSU. 0K; P2001) essing 15% — ee Fale ogg Toten iy kl thor cnn = wad Tee = (34% deswaeed bali ia sas be Lis 2.3.2 Sccoud gonomopbie plo feenisphicrarhediglagr! inher cured Brae rates i all wring of flo. tapos? neva Dt the Yet ele CLable 3). The woul Billed tke sas tom nthe it role wit Aa and AnD Saaine cahbtiny the uusioum and signet ‘the soca repalucive sisal tye ul 386 — 3908 P 01. The sgn sega 4 meg elon Tete tbe delkaantvin seit a. Ia. alge al he pci ve iene Ths interesting si at fn ooeceet tothe tm eyo the edet-cested AMD strait exiled Leiner lai adie O88 mane The str ily rats of Zoe amliophic es trom the AD, stout he th sigan, Inn Jats sib aay 05 tara hatea pot Spaale al 39 rela leat" ein the Ae seine The ter edleetd stnins alo shod ao. appineihie tection Ue got ekg ayy 12 bo 27 Tower tesa parol (7 0.3) { Tatly 3) Aad. Heaa { 3.bild seca to Aetourthrin eh saw ne 6 “ere! fey. This fuer stesies upon our feds tat ssprdutive am rag presi me hs able set of dati and selection seletus en te een a atcha Aner phen bth Bod eres cr Fi of da sed has aie mean ith be Seki wean Tele Ain, the Au ali Thad Tangs oipton poriod than the lel ersina, ‘Dough snifeanly vc difize (20.18% 2.3.3. Thi gountuphio exele Furacr reduction isthe ogg luvin cepocity as netieed in the Ind stmetopie ogee im all the eaune ofan, stops milk putlensiilar Uy the earlier eels Ths snosian ralavel Unite we served in An fran wilh 276 rcuoel “edly an rasa An stain and 214 a6 computed ta the 2nd cree Table 4 1. A reouskable bwervation ‘eas the shnionhed ge peection in AuD) grin vei Laid 268 less mamoboe ch oe in the Ted cyclo. A eduecd foouudity of S15. wus mice in the rellepluct sins Tod 4 ttt ofdcliametta nad macula dinette the female ad Linch of Anopheer ‘Sahenstin he 3 sonerobe ee Site tel oe essed fev the hatchalility agit sara syeine a nionguisne i well mer sf vorione tins woth the percent hats catalibed Comat er al, 2002, 20049. The evansing, bt apie Tee it AnD AnD aaina im eamparican m9 that tthe nalor fyclea The ArD, , suain proved to be tho lost ferule (5% ; among all the nl tzsine etutod. lee ollie elieralion tae lle 205 erased faulty 4 tho Aull, aris as compared 1 the prsmons eve (Table 43, Te addiiat, wo wauted Ueyones tn the osipation dein wan seam the Sit njele (P0051 DISCUSSION 3 4.1. Faesal/altsoseepbiley ad selection sti ‘he igh Sraeciodal potential of Actanethain proent ates olay vorlinmal the Taney of that ssi! Bebe al sti a te steohes, though the sunceaire rletious of peat sovcepl stn ee delanetrin alae eas ths Ueselaprin of at pimiscle Ive reine to Aearilucn, The etndie ake revealed that the 30 ‘ synerginal delkgeetirin along ith PRO thai relive the Teveln aid mynd of le omelhea entance vet ceed ot Hoe ut carpets. The inmestit ime mn egoskanve Wiss a le sovompibla and raionely-lectad “rtstant amine reveal tho fut thot tho deltmuctin-ceetnt ulti af ete lel sili ta opteduative ouintal: iy gonoeal wt an ms TRACP ke ooomatpon San ae apqrrsiable rumentivn Hiabanigee The scien sieges lad Teledtrn palure the myrindad've Bln of te silent. Rosia smrngenenl tris iy ly ft lm esl Rinse eis gevly rele Issel: genteel te it poser saver breyten beens sul evinkin hamueiten (Lean afl, TOR? ne resalant reprnhctoe isalsntage fs hyo igh signifsion in yeu vale i prolly slevree the rial vice fo te Tile over yareation. 3.2. Duration of gonotrophie estat Ghar Freliglions pve he hatin the snot goings wp le ole higher i the AS Sea cpm ble Alt sesso Ae, op (hi mss ile Foe he rly Pri i CT9YB > see vente gee Hie ele iv ta Telia tei allah A pd eters tle meted one Se, la a sivas Uy Te eos i a Doin ain ot Melanesian aegypti her ‘lec wil ella tiv Tnly sina eters hrs e208 3.3 Reproductive potential studies Hie audig abn puvvel tat deeiine resin Sng (60. TENE essen) sal Ane BS.94dd cenibtanes ) ain of alu at bioa Ievstnt ach Trae ful ADP ad Aa nie that ‘the Tarsbacrsted ating wth apnea ene a coupe be the ene wat cals iis Works of ebiliead ch ne aspreduntve daecantegs a8 compared 10 thse wit low ‘rele of resistance. Ons paobab.e reas. fr tho dod Eeeuodity inthe slot eae ae coupe to the parca maine uisy be the poartstion of Inwetinhe ino the ova imparting tsi effets om fhe sig File gow hich peseibly caused the iahibition of eyatain and tauepont of ge yolk wrotsine. I indicat a negative comlaton benecon the Ives of dshauralsis. lnval resistance nd the productive potoutil cf ls. wplens.Similr reults bore hoon aepottd by Prigalacsbia eal (1990) 2m an Taian stun of dn stephens wih ‘eiubited 32% — 40 doseass on feeunity rates ‘when tated wi mosh doaes of detain at the lnral stage. Mabapata et af (1999) las also spac significant doetease in to foomaity cates af ae saplensé. Cx. quinguefaciatr aud te acgsi fer eatuents with eyihihin and fndbrio, Tavlier. Worms (1986) sko seconded reduood fecundity of 40, 65, 43.38 and 67. 25. after selecton prestice of eptheids an Ja. stephens eo ony anal Ge grequnfiacinen reentvely (hie mls eam il Hae mgr Tei sei agp which eal 38. 7% reaver rsniy whe de fw oe Leth FeO grown brea! xg { heen, 2018? sions pallens Sill GIT-taM ste tamethrin moiniwe, Lat a {20021 epi 41S aro fer ll aes 2 pvmratinns oer mts let ln etn adi i nse vas thd he Any slain ih has ie Tevet clletis resin fal nak shan Ten Cline stn a Pemba rectvy ie ai, lh gi rose thal Uber ye cages Ween the Pepe es sal te Teva ae meth rien. fuer, the sales frie XD inthe Ta unto egre wn ep the ra rele, thunal eshte vehacel lsat a Mind pe sal thes regain Ue rulorellncsulily in suctoasive grndiphieselen he ers Pei wachst al see Bre skin ‘har siding seve han He x eeu moti ryt, tle paral ppt lial fiosimam habe in rtrd lhm meet flee losing inter mal ing gcillyfLaor rschas ‘le AnD ateain prowl Iw be te Teg tani ryclas exhililiny loreal pert Tore uf 509 bu 315) Prank of a2. 190} Thad evel 24% seis i th ity abe Ilia sea of os stn wl Ive ih sae Trill loam uf Dentin of aa nig Ow tae rutirys i a Aliana of clas fate, Okuse ceed. (ZN) dere 81-36% Teva Tet i the Inmtiniibreini les os ery ti 6.5 Teste seconded i Cee sancti arin, hn a vegso, Kuna wal £2009) repaid the ena ily iv sella flaca alndn br He il na gonatelic eyes wwlsteas in thid ele, the Teast fertile ain ropa war Fy emnerghed dcameslnin Teal select suai, Sipidicant seduction in te flisy sates of Indian stsive of Cx. guinguefascitas. Je argptt and fm aephnut ean tenotded aftr ‘meu to eyOutbein and fdhttain Mohapatra 2 al, 1989) "To wos Seti in AnD tan wih iehrst love of lured dekowethrin seeetaner £60, L-fol) vena indicates a ncystre impact of the lasoetiele revntance en the batebaity rote al lc, stn ‘The posable canto anny he the aeloation prose of lowoctcides at higher slosuge ss compured to the Hosuge subjecto to the Jesessstumt strains us elimsctcn bas een reported ts have delayed Ilia strain of de am Sin KIMAIL rat Ici csi protien be cial tbe i Ae ate? (Sabyal ant ll TOYS lng tle pstealion vl anes Ne exe aul oranda heel (Gur ses on Thal he Aly sis siete’ chelsea vey lve bevels ln Abnelti'n sealer sucansvr grams Hest slat tle aul sages bt tae float sluwoal rahoal Tsay sand Terai, Tug sas sont sin the rape the An tevin, ace that eaite the et tea te Sselaprent of baw revista it te sees ik aagerapetir, ae relation ia repulse i meinen a in aage hin ieeian ings al alvallevslivn see Ue cary uf deliver aes acute, Cala af hl seltion os ada tlyesevelenent ot sult sess i eas a te ality Uo dlanedluin selene he sau rman isalsatge i be sri abl ge te ig fem ich ori age ole fala snaked eb ne Taal sala ow af an spe wih sneginaldliogetin in tbe pearl atucien ual the Toe nepoulition Rlaew in Mad, ovine Mugged Tae sect wi ‘pve oal deli oly rea or eve Te deeds sisne Til slae_puiniden repnueiosslinalsantage to tle sin sad rales ite malrtve “aeens is any Vel in la Me freq sn ils sed ral Te vat i ling st fe pening eben Cr ngauiy ewate 4" Conclusion Oar Fewatigaions emplanioe te fae. Ua ela’ is ah oflertve iwectivile meant he uated af wala sail, Cats ae alton el vabetion maine i seas rane Oe Sselaprinl of sige level of ae aan ti deliv wbiel sul by alo her selene with neuen della. Despite the selon of ise in oar sis, eal Tale great pine a ean aye Twecanae af the sige io comet uf se bawelrin resstinos Tevet Teameat fread forty AND ain with Tighest Towel of ereal dshavetin verte and fhoecar invseated.aepredactee potential in AL wowed the comstion. Further, the eflears af Arenelscn as am adultinide and ability to cen reproductive potential of ack meaguit:es. ua Aull, ame, town appicetibc level soggont aut the 430 of dehowethrin c= 00 adn.ceide rather theo a lervicide agginst iv. stulenst 3 2 ute effcuse Peaneutine Fltean. he stray fe 8 rexitine eranagrener. eri ie leary agen) thal the ponchos lan resin gevelyjen ine ulti can clirinate Keeney ahd werent ouiaygece by iphucing diet seetan agent eto spurt ti. stapent. We Revel Test Tae lr got sae bth kewl Fe cal to one's Racin 97 bse ta PB au 69 get dd sole ais a ‘2 sean tide we Teton wen ined Ternshis. dst ard cement pede ‘uray ve resnuce of sumevito Iomie 0 Seeeiee BREN KERR REBAR AS ae BIE SHIRE a BS Suri KUMAR. MLK. K, DILLAT a OVATE EK RS" GOES MENG UOT ALA ES A TN TEN sip an MA IMEASAAGEE, Aa 12S 0 VRAIS se, MEER A UT [900 ) PARR TIMER AML URPATIS Ah BARA UST SHE TG REE ABET EC MMT PEA ALAM ERAN EH MEE TH RRR re, SAR BoE hk, Ani SAAD BRITS, 7 9S 75H aN PSE, AH EETPAN TALES, NN 1.88 0.968 OMG EAGER, AFIS PARAL GER SOA, MAHAL MS ZOTAR, SEAT RALS, Ay TERE CRERIET 9 20.08. WR TI FE, ey ANSI OUP, A ARS GAOIBIS ALE SPR MAR ERR Ua AURA AIRE CT FARA Hohe REBECA EEN, FAH Tb. ACL US EOL BS ER LR NSE PAHS. SRA, | SPRY, HARING, MIN} IDAL: Le

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