HYM. RES. J. Vol. 13(1), 2004, pp. 57-63 Report on a Collection of Bethylidae (Hymenoptera) from Central New Florida, USA, with Description of a Species of Lepidosternopsis Ogloblin Geane O. Lanes, Fernanda T. Gobbi, and Celso O. Azevedo Universidade Federal do Espirito Santo, Departamento de Biologia, Av. Marechal Campos 1468, Maruipe, 29.040-090 Vitoria, ES, Brazil; (COA) email: [email protected] — Abstract. Sixty species of Bethylidae of the genera Acrepyris Kieffer, Allobethylus Kieffer, Ape- nesia Westwood, Anisepyris Kieffer, Bakeriella Kieffer, Cephalonomia Westwood, Dissomphalus Ash- mead, Epyris Westwood, Goniozus Forster, Holepyris Kieffer, Laelius Ashmead, LepidosternopsisOg- loblin, Plastanoxus Kieffer, Prorops Waterston, Prosierola Kieffer, Pseudisobrachium Kieffer, Wiabde- pyris Kieffer, and Sclerodermus Latreille were collected, primarily in Malaise traps, at 12 sites in Orange Co., 3 in Seminole Co., one in Osceola Co. and one in Volusia Co., Florida, USA. Lepidos- ternopsis is recorded for the first time from the Nearctic Region and Lepidosternopsis irradiata Lanes and Azevedo, sp. nov., is described and illustrated. ThemaleofLepidosternopsisisdescribed for the first time. Taxonomic comments for some species are included. There are about 2,000 species of Bethy- Our goal was to survey the Bethylidae lidae worldwide, 204 of which are record- of Florida, to establish range extensions ed from the Nearctic Region. The few spe- and to analyze the taxonomic variation of cies whose biologies have been investigat- the studied species. The specimens we ed are idiobiont, or incipient koinobiont, studied represent 60 species, 18 of which ectoparasitoids (Finnamore and Gauld are different from the 52 recognized by 1995). They parasitize larvae ofColeoptera Evans and Fullerton (1997). Five genera: Wes- or Lepidoptera that live in cryptic situa- Allobethylus Kieffer, Cephalonomia tions. Most Bethylinae parasitize microle- twod, Lepidosternopsis Ogloblin, Plastanox- pidopterans, whereas most Pristocerinae "s Kieffer, and Prorops Waterston were not and Epyrinae attack beetles, especially observed by Evans and Fullerton (1997). those inhabiting wood or seeds (Evans Some species occurred in remarkable 1964) numbers, for example 739 males of Pseu- In the United States, Florida possibly disobrachium flaviventre (Kieffer). Lepidos- has the richest bethylid fauna. Beginning ternopsis is recorded for the first time from Cinent1r9a9l0,Fblioorliodgaisstsurvateytehde tUhneivaerrtshirtoypoodf ctihees'NweahriccthicwReegdiesocnribbaeseidn tohnisaPnaPeewr"spe- fauna of the campus (Evans and Fullerton TERMINOLOGY 1997) and other adjacent sites. Evans and Fullerton (1997) studied an assemblage of Terminology generally follows Evans 52 species of Bethylidae, based on approx- (1964). The nomenclature of integument imately 3,000 specimens from Central sculpture follows Harris (1979). The ter- Florida. We have studied an equally large minology of wing cells and veins follows second assemblage of Bethylidae in order Gauld and Bolton (1988). Abbreviations to better understand the fauna of this used for the description of the new species state. are: DAO, diameter of anterior ocellus, 58 Journalof Hymenoptera Research measured in frontal view; HE, height of 9. Orange Co., Orlando. UCF, Mackay eye, measured in lateral view, across its Tract, Swgrass Marh. Red Maple. maximum height; LFW, length of fore 10. Orange Co., Orlando. UCF, Cypress wing; LH, length of head, measured in Forest. frontal view, from the vertex crest to the 11. Orange Co., Orlando. UCF, Long Leaf median apical margin of the clypeus; Pine Sand Pine Turkey Oak. OOL, ocello-ocular line, measured in la- 12. Orange Co., Orlando. UCF, Long Leaf tero-dorsal view, the shortest distance Pine Saw Palmetto. from the eye top to the posterior ocellus; 13. Orange Co., Orlando. UCF, Maiden- VOL, vertex-ocular line, measured in lat- cane Marsh. eral view, the distance from the eye top to 14. Orange Co., Orlando. UCF, Pond Pine vertex crest; WF, width offrons, measured Comm. Dahoon Holly. in frontal view, its minimum width; WH, 15. Orange Co., Orlando. UCF, Sand Pine width of head, measured in frontal view, Rosemary Scrub. its maximum width including the eyes; 16. Osceola Co. Walt Disney World. WOT, width of the ocellar triangle, mea- World Drive/US 192 SOI T25S R27E. sured in frontal view, the maximum Sand Pine/Rosemary Scrub. width, including the ocelli. 17. Sarasota Co., MCC-Venice Campus. Long Leaf Pine-Saw Palmetto. COLLECTION SITES 18. Volusia Co., Daytona Beach. Urban- The material examined is deposited at Beachside, Halifax-River. the Entomological Collection of the Uni- LIST OF SPECIES versity of Central Florida, Orlando (Stuart M. Fullerton). It was colleUctVed using Mal- forEitghhetfsiprsetciteismoeffBreotmhylFliodraiedaa.reTrheecsoerdaerde aainsde strwaepesp,ipnigt bfaeltlwtereanps,1997 alnidght20t0r0apsi,n indicated with an asterisk (*). Listing of 18 sites in Central Florida in Orange, Sem- genera follows Evans (1978), with species inole, Osceola, Sarasota and Volusia of each genus listed alphabetically. Counties. The collection sites are referred Subfamily Bethylinae to by number in the list that follows. Goniozus columbianus Ashmead. 569, 18. Sites 1. Seminole Co., Econ Wilderness Area. 1, 6, 9, 10, 11, 13, 14. Scrub Oak/Saw Palmetto (burned). * Goniozus complanatus Evans. 39. Site 15. 2. Seminole Co., Econ Wilderness Area. Goniozus electus Fouts. 29. Site 13. 3. SScermuibnoOlaekC/o.S,awOviPeadlom.etto (unburned). GGoonn1ii1.oozzuussffllaovriipdeasnuFsou(tAs.sh2m0e6a.d)S.ite5s9.1,S2i,te6s, 99,, 1160., LK 4. Orange Co., Tibet- Butler Pre- Goniozusfratellus Evans. 16. Site 13. serve/Scrubby Flatwoods. Goniozusgracilicornis (Kieffer). 16. Site 11. 5. Orange Co., Orlando, Tibet Preserve Goniozus hortorum Brues. 1309, 2d. Sites 2, 6, 9, Myrtle Oak Scrub. 10, 11, 13, 14, 15, 16. 6. Orange Co., Walt Disney World. C- 4 Goniozus hubbardi Howard. 169,28. Sites 9, 13, Stout Site SI5, 16 T24S R27E. Xeric 14, 18. Oak/Flatwoods. Goniozus nigrifemur Ashmead. 989, 66. Sites 1, 7. Orange Co., Walt Disney Wolrd. MW- 2, 6, 8, 9, 10, 11, 13, 14, 16. 5 (unburned) S16 T24S R27E. Sand Prosierola bicarinata (Brues). 39. Sites 1, 4. Pine/Oak Scrub. Subfamily Epyrinae 8. Orange Co., Walt Disney World. MW- 7 (unburned) S22 T24E. Sand Pine/ Allobethylusfloridanus Evans. 79. Sites 9, 11. Oak Scrub. Anisepyris analis (Cresson). 1009, 39^. Sites 1, 2,6,8,9, 10,11,12,14. Volume 13, Number 1, 2004 59 Anisepyris columbianus (Ashmead). 349, 268. Acrepyris atra Klug. 3c?. Sites 1, 2, 11. Sites 6, 8, 9, 10, 11, 12, 14. Acrepyris bridwelliEvans. 17c?. Sites8, 11, 12, 14. Anisepyris grandis (Ashmead). 259, 211c?. Sites Acrepyrisfraterna Evans. 29, 45<?. Sites 8, 9, 10, 4, 5,' 6, 8, 9, 10, 11, 12, 14, 15, 16. ll,'l2, 14. Anisepyris subviolaceus Kieffer. 159. Sites 6, 8, Apenesia parapolita Evans. 59, 130c?. Sites 6, 8, 10, 11. 9, 10, 11, 12, 14, 16. Bakeriella mira Evans. 6c?. Sites 9, 11. Dissomplialusapertus Kieffer. 311c?. Sites8,9, 10, Cepmalonomia hyalinipensis Ashmead. 209, 2d. 11, 14. Sites 1, 9, 10^ 11, 14. Dissomplialus barberi Evans. 10c?. Sites 9, 11, 14. Cephalonomia perpusilla Evans. lie?. Sites 9, 11, Dissomplialus evansi Azevedo. 34c?. Sites 6, 8, 9, 14. 10, 11, 12, 13, 14, 16. * Ceplmlonomia quadiceps Evans. 19. Site 6. Dissomplialus kansanus Evans. 2c?. Sites 6, 10, 11. * Cephalonomia conophthori Evans. 29. Site 9. Dissomplialus krombeini Azevedo. 3<?. Sites 11, Epyris californicus (Ashmead). 198. Sites 9, 11, 14. 14, 16. Pseudisobrachium arenarium Evans. 75c?. Sites 2, * Epyris corticinns Evans. 199. Sites 9, 10, 11. 5, 6, 7, 8, 11, 12, 14, 16. Epyris deficiens Krombein. 69, 778. Sites 9, 10, Pseudisobrachium ashmeadi Evans. 10c?. Sites 11, 11, 12^ 14. 12, 16. Epyrisfestivus Evans. 279, 10(5. Sites 9, 11, 12. Pseudisobrachium carolinianum Evans. 77c?. Sites Epyris oriplanus Kieffer. 18. Site 6. 6, 7, 16. Epyris rufipes (Say). 189, 35c?. Sites 1, 11, 12, 14. Pseudisobrachium flaviventre (Kieffer). 739c?. Epyris spissus Evans. 429, 338. Sites 6, 8, 9, 10, Sites 4, 6, 7, 8,9, 11, 12, 14, 16. E*pHyo1r1li,esp1yt3rr,iisc1o4sc,taat1ta6nl.sinEaveaEnvsa.ns7.9.29S.iteSsit9e,s1110., 11. PseSuidteisso1b,r6a,c7h,iu8,m11r,uf1i2v,en1t4r,e16(.Ashmead). 25c?. Holepyris floridanus (Ashmead). 129, 9c?. Sites NOTES ON INDIVIDUAL SPECIES 9, 10, 11, 12, 14, 17. Holepyris graminis Evans. 286. Sites 2, 6, 10, 11, Some individuals of species in the new 12,14,16. series from Florida presented previously Holepyris habilis Evans. 79. Sites 9, 10, 14. unknown variation. In these cases, we Holepyris lautns Evans. 19, 62d. Sites 2, 6, 9, 10, comment upon them below in order to 11,'12, 14, 15, 16. help better understand morphologicalvar- Holepyris micidus Evans. 129. Sites 2, 9, 10, 11, iation within the species. Hol11e44p.,y'^r1i5s. subtilis Evans. 19c?. Sites 6, 10, 11, 12, Acreeypeysriwsitahrmmifienruate(Sasye)t:ae.males may have Laelius centratus (Say). 139,18. Site 7, 9, 10, 11, Anisepyris analis (Cresson): antennal seg- 13, 14. ments III+IV can be 3.OX as long as Lepidosternopsis irradiata Lanes and Azevedosp. thick. nov. 3c?, 49. Site 9, 10, 11, 13, 14. Anisepyris columbianus (Ashmead): this * Plastanoxus laevis (Ashmead). 18. Site 1. species is similar to A. williamsi Evans ** PPrroorrooppss anbassoulteataEEvvaannss..629c,?.3cS?i.teSsit9e,s194,. 10, 11. finemtahleesc,olbourtotfhleeglsowaenrdfsocvuelaptoufrethoefmteh-e Rhabdepyris amabilis Fours. 55c?. Sites 6, 10, 11, 14, 16. sopleuron of the former species is divid- Rhabdepyris carolinianus Evans. 12c?, 29. Sites8, ed by a conspicuous septum, whereas in 9, 11,'14, 15. A. williamsi the lower fovea is not divid- Rhabdepyris muesebecki Evans. 59, 80c?. Sites 9, ed. Males of A. columbianus and A. wil- 10, 11, 14. liamsi have this septum top-flatted. Scleroderma macrogaster Ashmead. 249. Sites 3, Anisepyris grandis (Ashmead): the septum 9, 11, 13, 14. of the lower fovea of the mesopleuron Subfamily Pristocerinae varies from narrow to thick in the fe- Acrepyris armifera (Say). 29, 35c?. Sites 8, 9, 10, males. The frons also varies in width. ll,'l2, 14. ' Males can have the head with green or 60 Journal of Hymenoptera Research blue reflections, and some with reddish Laelius centratus (Say): females can have legs. legs light castaneous. ^ Cephalonomia conophthori Evans: the pro- Plastanoxus laevis (Ashmead): this wide- podeal disc is 1.25X as long as wide. spread species is recorded for the first Cephalonomia hyalinipennis Ashmead: some time from Florida. females are light castaneous and the Prorops obsoleta Evans: this species was head varies in length, some have the known only from 2 males and 1 female head longer or with side parallel behind from Trinidad. the eyes. Prorops nasuta (Say): in the Nearctic Re- Cephalonomia quadriceps Evans: this species gion this species was known only from was known from 3 males from Massa- 1 male from California. chusetts, Maryland and North Carolina, Pseudisobrachium arenarium Evans: the USA. width of the male frons ranges from Epyris corticinus Evans: this species was 1.30 to 1.40X height of eye and propo- known from Virginia, Maryland and deal disc from 1.60 to 1.70X as long as Pennsylvania, USA. In this series, some wide. females have small scutellar pits. Pseudisobrachium ashmeadi Evans: length of Epyris deficiens Krombein: males can have propodeal disc in the males can be poorly defined longitudinal striae. shorter than in the type series, 1.26X as Epyris rufipes (Say): some females canhave long as wide. the propodeal disc evenly striate and Pseudisobrachium flaviventre (Kieffer): in the median transverse vein not males the head varies from dark casta- strongly oblique. neous to black, the antennae from light Goniozus columbianus Ashmead: males can to dark castaneous, the diameter of the have the head weakly coriaceous. anterior ocellus varies from 0.17 to Goniozus complanatus Evans: this species 0.23X the width of the frons, and the was known only from Texas, USA. mesopleuron with callus ill defined. Goniozus hortorum Brues: female heads Psedisobrachium rufiventre (Ashmead): this vary in length, punctures size, frons tex- species is very similar to P. flaviventre, ture, clypeus length, sculpture, and differing by having the discoidal vein height of median carina of slightly conspicuous, the antennae with clypeus. Males can have the cubital vein 2.5X as segments slightly longer and propodeal long as wide. disc slightly longer. Males can have the Goniozus hubbardi Howard: the third an- mesopleuron with callus well defined. tennal segment can be 1.5X as lone as Scleroderma macrogaster Ashmead: the pat- thick. tern of body coloration shows a large Goniozus nigrifemur Ashmead: females can range of variation, but with the meta- ghraavntes.theMaalnetsenncaaenashasvheortthaes ianntG.enenmaie- hseoamdaccaonnsbteandtalrykdcaarsktanceaosutsa,nedoiusst.incTthley pwrteoeelsnaltnc-eahiri1nindosgirecgtachmtaeeerdni.vntearatsoefxlotchnreegstap,srotthpheoicdtkeh,ailrodrdatinhs-ce btadheanordrdkaiesmxrteicstnaochntpallnbyeeutdhreaeovrnekt,nehlroyraaantlxdi;tghhtttehhceemaesthstheaoonasredacoxuutasc.unadmn Holepyris catalinae Evans: this species was Lepidosternopsis irradiata Lanes and known only from Arizona, USA. Azevedo, sp. nov. Holepyris graminis Evans: males can have Figs. 1-8 wtheeakplosytedreivoerlgorpeodo.ve of the pronotal disc FMWale1.6hmolmo.tyCpoel.o—rB:oDdayrklecnagstthane2o.u4s;mamn;- Volume 13, Number 1, 2004 61 £^> 8 Figs. 1-8. Lepidosternopsis irradiate sp. nov. 1—4, Male. 5-8, Female. 1, Head and mesosoma, dorsal. 2, Man- dible, frontal. 3, Forewing, dorsal. 4, Genitalia, ventral. 5. Head and mesosoma, dorsal. 6, Mandible, frontal. 7, Forewing, dorsal. 8, Metasoma, ventral. (Scale bars = 0.32 mm). 62 Journal of Hymenoptera Research tenna, mandible, legs and palpi casta- with apex rounded; volsella with cuspis neous; wings hyaline, veins castaneous. laminar, very wide, slightly shorter than Head (Fig. 1): Mandible with 4 apical paramere, ventral margin excavate in api- teeth, the lower one relatively bigger and cal half; aedeagus bottle-shaped, progres- sharper (Fig. 2). Clypeus with truncate sively slender apically, apex emarginated, median lobe, with median carina concave basal margin rounded, in profile. First four antennal segments in Female.—Body length 2.8 mm; LFW 1.9 ratio of 12:6:3:4; segment III 0.8X as long mm. Similar to male except: Color: Head as thick; segment XI 1.6X as long as thick; and thorax castaneous, except the prono- antennal sockets closed to each other, sep- turn light castaneous and propodeum yel- arated by 0.3X their diameter. Eye with lowish anteriorly; metasoma dark casta- sparse, short setae. Frons weakly coria- neous; antennae and palpi light casta- ceous, shinning, with small, very sparse neous; mandibles and legs castaneous; punctures, with shallow and small groove wings hyaline, vein light castaneous. between the antennal sockets. LH 1.08X Head (Fig. 5): Mandible with 3 apical WH; WF 0.58X WH; WF 1.12X HE; OOL teeth, the higher very shorter than lowers 0.85X WOT; DAO 0.41X WOT; frontal an- (Fig. 6). Clypeus very short, concave me- gle of ocellar triangle acute; posteriorocel- dian lobe, with median carina weakly lus distant from the vertex crest 0.5X high and concave in profile. First four an- DAO. Vertex slightly convex with corner tennal segments in a ration of25:8:4:4; seg- somewhat angled. VOL 0.85X HE. Head ment III as long as thick; segment XI 0.7X not flattened, its thickness 0.62X LH. Me- longer than thick; antennal sockets close to sosoma (Fig. 1): Thorax slightly coria- each other, separated by 0.6X their diam- ceous. Pronotal disc 1.25X as long as me- eter. Front very weakly coriaceous, shin- soscutum, with transverse carinae very ning. Head globoid, 0.58X as high as long, weak. Notauli very weak, occupying an- LH 1.15X WH; WF 0.37X WH; WF 1.30X terior third of the mesoscutum; parapsidal HE; OOL 2.06X WOT; DAO 0.3X WOT; furrow absent; scutellar groove slender, posterior ocelli distant from the vertex Propodeal disc 1.12X as wide as long, 1.4X DAO. Vertex slightly convex, with weakly coriaceous, shinning; without me- corner somewhat angled. Temples slightly dian and posterior carinae, with lateral ca- converging anteriorly. VOL 1.20X HE. rinae, lateral margins straight; declivity Mesosoma (Fig. 5): Thorax coriaceous, without median carina; propodeal spiracle Pronotal disc 1.72X longer than mesoscu- totally directed outward. Mesopleuron turn; notauli very weak and inconspicu- weakly coriaceous, with wide, shallow fo- ous; parapsidal furrow, incomplete ante- veae occupying nearly all surface, with a riorly, occupying more than half of the small central pit. ForeMwing (Fig. 3) with mesoscutum. Propodeal disc as long as veins Sc+R, Rs+M, +Cu and lCu-a, wide, coriaceous, lateral margins concave forming only one closed cell (median), anteriorly. Mesopleuron slightly coria- Fore femur 2.1X as long as wide. Median ceous, with large and shallow fovea oc- tibia not spinose. Claws simple. Metaso- cupying surface, with small central pit. ma: posterior margin of sternites IV-VI Fore wing as for male (Fig. 7). Fore femur biemarginated. Hypopygium with poste- 1.94X as long as wide. Metasoma: 1.73X rior margin narrow and concave. Genitalia as long as the mesosoma. Posteriormargin (Fig. 4): Paramere completely divided in of sternites IV-VI biemarginated (Fig. 8). two arms, ventral arm wide, with apex Type material—Holotype 6, USA, Flor- somewhat truncate in ventral half, dorsal ida: Orange Co., Orlando, UniversityCen- margin convex, ventral margin straight, tral Florida, Cypress Forest, 2.VI.1999, dorsal arm shorter than ventral, slender, Malaise trap, P. Russell and S. Fullerton Volume 13, Number 1, 2004 63 col. (deposited in Entomological Collec- described the male of this genus for the tion, University of Central Florida, Orlan- first time. L. irradiata differs from all the do). Allotype 9, USA, Florida: Orange known species of the genus, because it is Co., Orlando, University Central Florida, macropterous with fore wings having MacKay Tract, Sawgrass Marsh, Red Ma- only the median cell closed and radial ple, 14.VI.1999, Malaise trap, P. Russell vein absent. and S. Fullerton col. Paratypes, USA, Flor- ACKNOWLEDGMENTS tirdaal:FOlroraindgae: 1Co6.,,SOarwlgarnadsos,MUanrivsehr,siRteydCMean-- anWdethtehdaunpkliSc.atFeulslreerttaoinnefdo.r the loan of the material ple, 13.IX.1999, Malaise trap, P. Russell LITERATURE CITED and S. Fullerton col.; 16" and 19, LLP- Sand Pine, Turkey Oak, 7.XI.1997 and Azevedo, CO. 1999. Additions to the Neotropical h2F.uoVloIlnIe.r1tH9o9on7l,lcyoMl,.a;l1a11i9.s,Ve.P1ot9rn9ad9p,,, PPM.ianlReausiCssoeemllmt.ra,anpdDaPS-.. EvansEsf,cprryoHirmp.itEnBi.raoane1zs9i6l(4.oH.fyIhAmaeernisnnyogenpiowtap,esrssiaepsr,eiceoifBeZeosttohhleoyoflgAiimdEaeaeerp8)i7id,:co1as1wtn-ie1trB8nh.eotphdsyei--s Russell and S. Fullerton col.; 19, Maiden- lidae (Hymenoptera, Aculeata). Bulletin of the cane Marsh, 17.V.1999, Malaise trap, P. Museum ofComparative Zoology 132(l):l-222. RusEsteylmlolaongdy.S—. TFuhlelenratomnecorle.fers to the ab- EvansoI,nfstMHie.txuitEec.o21.79:7M18e-.3m3oT2ih.resBoeftthhyeliAdmaeeriocfanAmEenrtiomcoalongoirctahl sence of the —radial vein in the fore wings. Evans, H. E. and S. M. Fullerton. 1997. Report on a Discussion. The description of the ge- collection of Bethylidae (Hymenoptera) from nus was based on an Central Florida. Proceedings of the Entomological Lepidosternopsis ap- terous female of L. kuscheliana Olgloblin, FinnSaomcoireety,oAf.WaasnhdinIg.tDo.nG9a9u(1l)d:.17149-9157.9.Bethylidae, p. 1953, from Masatierra Island (Chile). 470-479. In: Hanson, P. AND I. D. Gauld. The Evans (1964) described two species based Hymenoptera ofCosta Rica. Oxford, Oxford Uni- on micropterous females from Australia. versity Press, 893p. Azevedo (1999) described the first mac- GaulOdx,foI.rdD,.OaxnfdorBd.UBnoilvteorns.it1y98P8r.essT.he33H2ympepn.optera. ropterous species ofLepidosternopsis,based Harris, R.A. 1979. A glossary of surface sculpturing. on two females from Para, Brazil. Now we Occasional Papers in Entomology28:1-31.