P T , ROTEIN ARGETING TRANSPORT & TRANSLOCATION This Page Intentionally Left Blank P R O T E I N T , A R G E T I N G T & R A N S P O R T T R A N S L O C AT I O N Edited by R E. D OSS ALBEY Ohio State University,Columbus,USA G H UNNAR VON EIJNE Stockholm University,Sweden Amsterdam London Oxford New York Boston San Diego San Francisco Singapore Sydney This book is printed on acid-free paper. Copyright 2002,Elsevier Science Ltd.All Rights Reserved No part of this publication may be reproduced or transmitted in any form or by any means,electronic or mechanical,including photocopying,recording, or any information storage and retrieval system,without permission in writing from the publisher. Academic Press An Elsevier Science Imprint Harcourt Place,32 Jamestown Road,London NW1 7BY,UK http://www.academicpress.com Academic Press An Elsevier Science Imprint 525 B Street,Suite 1900,San Diego,California 92101-4495,USA http://www.academicpress.com ISBN 0-12-200731-X Library of Congress Catalog Number:2001094562 A catalogue record for this book is available from the British Library Typeset by Charon Tec Pvt.Ltd,Chennai,India Printed and bound in Italy by LEGO,Vicenza 02 03 04 05 06 07 08 LE 9 8 7 6 5 4 3 2 1 C ONTENTS Contributors vii Foreword xi Günter Blobel Preface xv Ross E.Dalbey and Gunnar von Heijne 1 Introduction/Overview 1 Ross E.Dalbey and Gunnar von Heijne 2 Methods in Protein Targeting,Translocation and Transport 5 Ross E.Dalbey,Minyong Chen and Martin Wiedmann 3 Targeting Sequences 35 Gunnar von Heijne 4 Protein Export in Bacteria 47 Arnold J.M.Driessen and Chris van der Does 5 Protein Sorting at the Membrane of the Endoplasmic Reticulum 74 Nora G.Haigh and Arthur E.Johnson 6 Membrane Protein Insertion into Bacterial Membranes and the Endoplasmic Reticulum 107 Andreas Kuhn and Martin Spiess 7 Disulfide Bond Formation in Prokaryotes and Eukaryotes 131 James Regeimbal and James C.A.Bardwell 8 The Unfolded Protein Response 151 Carmela Sidrauski,Jason H.Brickner and Peter Walter 9 Protein Quality Control in the Export Pathway: The Endoplasmic Reticulum and its Cytoplasmic Proteasome Connection 180 Zlatka Kostova and Dieter H.Wolf 10 Translocation of Proteins into Mitochondria 214 Thorsten Prinz,Nikolaus Pfanner and Kaye N.Truscott vi Contents 11 The Import and Sorting of Protein into Chloroplasts 240 Jürgen Soll,Colin Robinson and Lisa Heins 12 Import of Proteins into Peroxisomes 268 Suresh Subramani,Vincent Dammai,Partha Hazra, Ivet Suriapranata and Soojin Lee 13 Nucleocytoplasmic Transport 293 Dirk Görlich and Stefan Jäkel 14 Protein Transport to the Yeast Vacuole 322 Todd R.Graham and Steven F.Nothwehr 15 The Secretory Pathway 358 Benjamin S.Glick 16 Vesicular Transport 377 Joachim Ostermann,Tobias Stauber and Tommy Nilsson 17 Conclusion/Perspective 402 Ross E.Dalbey and Gunnar von Heijne Index 407 C ONTRIBUTORS James C.A.Bardwell,Department of Molecular,Cellular and Developmental Biology,University of Michigan,Ann Arbor,Michigan 48105-1048,USA Jason H.Brickner,Department of Biochemistry and Biophysics, University of California,San Francisco,California 94143-0448,USA Minyong Chen,Department of Chemistry,The Ohio State University, 100 West 18th Avenue,Columbus,Ohio 43210,USA Ross E.Dalbey,Department of Chemistry,Ohio State University, 305 Johnston Laboratory,176 W Nineteenth Avenue,Columbus, Ohio 43210,USA Vincent Dammai,Section of Molecular Biology,Division of Biology, University of California,San Diego,La Jolla,California 92093-0322,USA Arnold J.M.Driessen,Department of Microbiology,Groningen Biomolecular Sciences and Biotechnology Institute,University of Groningen,Kerklaan 30,9751 NN Haren,PO Box 14,9750 AA Haren, The Netherlands Benjamin Glick,Department of Molecular Genetics and Cell Biology, Cummings Life Science Center,University of Chicago,920 E 58th Street, Chicago,Illinois 60637,USA Dirk Görlich,Zentrum für Molekulare Biologie Universität Heidelberg (ZMBH),Im Neuenheimer Feld 282,69120 Heidelberg,Germany Todd R.Graham,Department of Biological Sciences,Vanderbilt University,Nashville,Tennessee 37235-1634,USA Nora G.Haigh,Department of Medical Biochemistry and Genetics, Texas A&M University System Health Science Center,116 Reynolds Medical Building,1114 TAMU,College Station,Texas 77843-1114,USA viii Contributors Partha Hazra,Section of Molecular Biology,Division of Biology, University of California,San Diego,La Jolla,California 92093-0322,USA Lisa Heins,Botanisches Institut der Christian Albrechts Universität, Am Botanischen Garten 1-9,Olshausenstrasse 40,24098 Kiel,Germany Stefan Jäkel,Zentrum für Molekulare Biologie,Universität Heidelberg (ZMBH),Im Neuenheimer Feld 282,69120 Heidelberg,Germany Arthur E.Johnson,Department of Medical Biochemistry and Genetics, Texas A&M University System Health Science Center,116 Reynolds Medical Building,1114 TAMU,College Station,Texas 77843-1114,USA Zlatka Kostova,Institut für Biochemie der Universität Stuttgart, Pfaffenwaldring 55,70569 Stuttgart,Germany Andreas Kuhn,University of Hohenheim,Institute for Microbiology and Molecular Biology,Garbenstrasse 30,D-70599,Stuttgart,Germany Soojin Lee,Section of Molecular Biology,Division of Biology,University of California,San Diego,La Jolla,California 92093-0322,USA Tommy Nilsson,Cell Biology Programme,European Molecular Biology Laboratory,Meyerhofstrasse 1,D-69012,Heidelberg,Germany Steven F.Nothwehr,Division of Biological Sciences,University of Missouri,Columbia,Missouri 65211,USA Joachim Ostermann,McGill University,Department of Anatomy and Cell Biology,3640 University Avenue,H3A 2B2,Montreal,Quebec,Canada Nikolaus Pfanner,Institut für Biochemie und Molekularbiologie Universität Freiburg,Hermann-Herder-Strasse 7,79104 Freiburg,Germany Thorsten Prinz,Institut für Biochemie und Molekularbiologie Universität Freiburg,Hermann-Herder-Strasse 7,79104 Freidburg,Germany James Reigembal,Department of Molecular,Cellular and Developmental Biology,University of Michigan,Ann Arbor, Michigan 48105-1048,USA Colin Robinson,University of Warwick,Department of Biological Sciences,Coventry CV4 7AL,UK Contributors ix Carmela Sidrauski,Department of Biochemistry and Biophysics, University of California,San Francisco,California 94143-0448,USA Jürgen Soll,Botanisches Institut der Universität Kiel,Am Botanischen Garten 1-9,24118 Kiel,Germany Martin Spiess,Department of Biochemistry,Biozentrum,University of Basel,Klingelbergstrasse 70,CH-4056 Basel,Switzerland Tobias Stauber,Cell Biology Programme,European Molecular Biology Laboratory,Meyerhofstrasse 1,D-69012,Heidelberg,Germany Ivet Suriapranata,Section of Molecular Biology,Division of Biology,University of California,San Diego,La Jolla,California 92093-0322,USA Suresh Subramani,Department of Biology,University of California, San Diego,La Jolla,California 92093-0322,USA Kaye N.Truscott,Institut für Biochemie und Molekularbiologie Universität Freiburg,Hermann-Herder-Strasse 7,79104 Freiburg, Germany Chris van der Does,Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen,Kerklaan 30,9751 NN Haren,PO Box 14, 9750 AA Haren,The Netherlands Gunnar von Heijne,Department of Biochemistry,Arrhenius Laboratory, Stockholm University,S-106 91 Stockholm,Sweden Peter Walter,Department of Biochemistry and Biophysics,University of California,San Francisco,California 94143-0448,USA Martin Wiedmann,Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center,1275 York Avenue, New York,New York 10021,USA Dieter H.Wolf,Institut für Biochemie,Universität Stuttgart, Pfaffenwaldring 55,70569 Stuttgart,Germany