NOTE TO USERS This reproduction is the best copy available. ® UMI Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. PROPERTIES OP BOOTS SISRA IS ietpanoscma aw isi infbchok by Sfiohael George lyseako A Dissertation Submitted to the ftnutuate Faoulty is Partial Fulfillment of The Requirements for the Degree of DOGIQR OP PHILOSOPHT ^ Major Subject* Protoaoology Approved* Iowa State College 1950 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. UMI Number: DP12241 INFORMATION TO USERS The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleed-through, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. ® UMI UMI Microform DP12241 Copyright 2005 by ProQuest Information and Learning Company. All rights reserved. This microform edition is protected against unauthorized copying under Title 17, United States Code. ProQuest Information and Learning Company 300 North Zeeb Road P.O. Box 1346 Ann Arbor, Ml 48106-1346 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. Gs, L B 4 , F S' u . U 3 '5 f CABSI 01* e«3M © Pag# ................... mmoDvcnm*..,. 1 m m OP LITERATURE........................................................ 3 K B iia m M im # . ................................. 15 ® e a e r a l . . * ......... 15 Sodium Salicylate end Ablastio Plasma... 18 Proteins and Ablastio Serum. .................................. 22 Tryptophan and Sodium Salioylate Activity. ............ 26 Pantothenic Acid and Ablastio Serum. ...................... 27 Glucose Levels and Ablastio Blood ......... SO SISULTS AID DISCISSIOI.. ............... 52 Sodium Salioylate and Ablastio Plasma.*......... 32 Proteins and Ablastio Serum..*......................... 40 TPyptophan and Sodium Salioylate Activity. ................... 50 Pantotbenie Aoid and Ablastio Serum............................... 55 Glucose Levels and Ablastio B lo o d .............................. 55 SWMAtr AID COICLUSKMS ...................................... 86 LITERATURE CITED,..................................................... 72 ACMOWLBHMSflS............................................................ 78 T 9 5 0 9 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. H I, IX8T OP mBLSS Table Pag* 1* Percentage of division forms in rat* injected with (1) sodium salicylate infected rat plasm, (2) i n n t plasm, and (3) s a lin e ........................................... 34 2. Percentage of division forms in rats Injected with (1) liaaune plasm treated with aodium salioylate, (2) immune plasma, and (3) salin e...,............................................... 36 3* Percentage of division forms in rats in­ jected with dialysed plasm from sodium salicylate treated and non-treated infected rats* ........... 38 4* Summary of statistics comparing serum protein levels among (1) T, lewial infected rats, (2) sodium salioylaFe irealed Infected rats, (3) sodium salicylate treated, and (4) normal ra ts .........................• • • • • • • • 42 5. Bleetrophoretie analysis of pooled samples of sera from (1) salioylate treated infeoted rata, (2) T. lewial infeoted rats, end (3) normal ralsTTT..,................. 4? 6. Percentage of division forms and protein levels in rats infected with T. lewis1, receiving salioylate and plamm, "andf of controls .......... 49 7. Percentage of division form of trypanosomes in rats receiving (1) tryptophan plus sodium salicylate, (2) sodium salicylate only, and (3) untreated controls*. «... 52 8. Summary of statistics on pantothenic acid assays of sera of rats with (l) T. lew!si and salicylate, (2) J . lewisi, (1?) salicyl­ ate, and (4) of normal r a ts ..................... 36 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. ir. Sabi# Pag® 9. Summary of means in Experiment 9 ................... 88 10. Summary of statistics of blood gluoos© doterminations on rats with (1) T. lewial and salioylate, (2) f. lewisi, T( j) sal1 ey late, and (4) of"* normal r a ts .........................* ...... €0 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. LIST OP FiaUBBS Figure Page 1* Electrophoretic patterns of pooled •era of normal rats (top), rats with lewisi (middle), and rats with ft I'ewisT and sodium salicylate TreaBnF (bottom)*..................................... 45 Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. INHOBUGHOI Shore has been described in Ifryganosoaa lewlsl infeetions in rats an issmme reaetion that operates in two wayst first, an anti- reproductive antibody is formed whieh, inhibits reproduction of the parasites} then, a second antibody is formed whieh destroys the trypanosomas. Ihltaferro (1932a) sailed the first antibody ablastin and the second the trypanocidal antibody. She question of the existence, formation, and operation of ablastin has received a great deal of attention by many investi­ gators. S&liaferro (1924, 1925, 1926, 1932a) reported studies on the properties of ablastint he said that it was a protein in the euglobulin fraction of senna, it was passively transferable, it would not unite with trypanosomas in vitro, and that it neither killed the parasites nor affected their motility or vitality. Its only manifestation seemed to be that of In vivo suppression of reproduction. Blood sugar studies by Sohern (1928) and Poindexter (19S8) indicated an inverse relation between the amount of sugar and mmfcer of trypanosomas in pathogenic trypanosomiases• Linton (1929) found that in the non-pathogenie jg. lewlai there was no change in the blood sugar during the course of the infection. In the laboratories at Iowa State College, Booker and co-workers (1943, 194?) reported that deficiencies in Reproduced with permission of the copyright owner. Further reproduction prohibited without permission. pantothenic acid affected the antireproductive activity of ablastin so that reproduction continued and the infection was pathogenic. Sector and S&llagher (1947) found that treatment of infected rate with sodium salicylate had a similar effect on ablastio activity* Minoru (1940) reported changes in blood sugar levels in rabbits as a result of sodium salicylate administration* In low amounts them was a hypoglycemia* In greater amounts there was hyporglyoeaia* Brown (IfIS) and Taliaferro (1926) suggested that the differ­ ence between pathogenic and con-pathogenic trypanosomas might be based on the absence or presence of an antireproduotive factor in the reaction of the host. The relationships among pathogenicity, entireproductive activity, glucose utilisation tod glucose levels, pantothenic acid deficiency, and sodium salicylate treatment suggested that further studies be made on immune sera with respect to these various factors* In this investigation, therefore, are reported studies on immune sera of rats both treated with sodium salioylate and not treated with respect to (1) ablastio activity, (2) changes in protein levels, (S) pantothenate levels and (4) blood glucose levels* Reproduced with permission of the copyright owner. Further reproduction prohibited without permission.