INTERNATIONAL 10/11 PRODUCT CATALOG HPLC POST-COLUMN DERIVATIZATION | INSTRUMENTS / COLUMNS / METHODS / REAGENTS THE CONVENTIONAL WISDOM SAID IT COULDN’T BE DONE. BUT PROFOUND NAIVETÉ LED ME TO THINK “HOW COULD I NOT SUCCEED.” WE THEN SET ABOUT REVOLUTIONIZING AMINO ACID ANALYSIS. Previously you could do amino acid analysis only with a dedicated amino acid analyzer which has almost no fl exibility. We developed an eluant/column combination and the hardware necessary to transfer the analysis to a modern gradient HPLC. The result was a method with the most fl exibility that could evolve with the hardware and thus benefi t from each generational improvement. Thus the user may always have the benefi ts of the latest HPLC technology. Even today, dedicated amino acid analyzers execute the same method as when introduced in the late 50’s. MICHAEL V. PICKERING, PH.D. / FOUNDER As we enter a new decade, I am drawn back to the beginning. After I finished two postdoctoral positions in medicinal chemistry, I worked four years in R&D at two different HPLC instrument companies. During that time, I came 01 to the realization that the end user does not want capital equipment, solvents, chemicals, or computers. They want reliable chromatograms. As Aristotle’s theory on causality teaches, the final cause comes first. In this instance, the final chromatogram drives the user’s choice for equipment and chemistry. So convinced was I of this truth, I jumped into the void of self- employment. Our product would be the ethereal “Guaranteed Chromatography.” I started Pickering Laboratories in 1980, with the patented ninhydrin reagent TRIONE®. Soon thereafter we began offering gradient Amino Acid Analysis methods that were achievable with modern HPLC equipment. Back then, Amino Acid Analysis run times were 140-minutes for Lithium cation-exchange (native fluid analysis) and 90-minutes for Sodium cation-exchange (hydrolysate analysis). Today, the same chromatograms are achievable in 70-minutes for Lithium and 30-minutes for Sodium. As you are all aware by now, we work hand in hand with the AOAC, FDA, USDA and USEPA. The relationships we’ve cultivated over the years with these regulatory agencies have enabled us to influence analytical methods as they develop, and to better understand and support you. In the same context, we are regularly invited to make custom formulations and develop pharmaceutical QC methods. Our contribution to these regulatory agencies was recently recognized in September of 2009, at the 125th Anniver- sary meeting of the AOAC International in Philadelphia. Pickering Laboratories received AOAC’s Single Labora- tory Validation of the Year Award for Multiresidue Mycotoxin Analysis in Corn Grain by High-Performance Liquid Chromatography with Post-column Photochemical and Chemical Derivatization. Several colleagues and I attended the award ceremony – it was an honor for Pickering Laboratories. In fact, the award was doubled to include our Single-Laboratory Validation for the Quantification of Neomycin B and Neomycin C in Animal Feeds by Liquid Chromatography Fluorescence Detection with Post-column Derivatization. Well, here we are, 30 years later. My belief in Aristotle’s theory has turned out to be true. The relationship we have created has knit us together as a team: you observe/we support. A new range of products is presented in this catalog, so I encourage you to scan it for a solution for your needs. Or, call and ask for our advice. Your loyalty is our greatest reward. PRODUCT CATALOG 10/11 PICKERING LABORATORIES TABLE OF CONTENTS INTRODUCTION 53 Hypochlorite Diluent for Glyphosate 61 Multi-Residue Mycotoxin Herbicide Analysis and Amino Acids 61 Afl atoxins 3 Customer Service and Technical Support 53 ChlorAC 61 Polyether Antibiotics 3 How to Place an Order 54 Restore 61 Paralytic Shellfi sh Toxins 4 Factory Repairs and Limited Warranty 54 Pure Water 5 Instrument Service Plans BECKMAN 6300/7300 54 Extraction Solution INSTRUMENTS, HARDWARE, 54 Samples and Standards Diluents 62 Analytical Columns and Guards REACTORS & FLOW CONDITIONERS (Li220 & Na220) 62 Eluants 7 Pinnacle PCX Derivatization Instrument 55 Calibration Standards and Test Mixtures 62 Reagents and Chemicals 10 Vector PCX Derivatization Instrument 57 Artifi cial Perspiration REPLACEMENT COMPONENTS, 12 CRX400 Post-column Reactors FITTINGS & TUBING ELUANTS 12 Flow Conditioner 63 Pinnacle PCX Flow Diagram– 13 Flow Restrictors 58 Sodium-based Solutions for Amino Two-Pump System 13 Glyphosate & AMPA Clean-up Acid Analysis 64 Pinnacle PCX Flow Diagram– 58 Lithium-based Solutions for Amino CHROMATOGRAMS, One-Pump System Acid Analysis COLUMNS & GUARDS 65 Vector PCX Flow Diagram 58 Glyphosate Herbicide Analysis 15 Columns & Guards Catalog Information 66 PCX5200 Flow Diagram 58 Potassium-Based Solutions for 16 Cation-exchange Columns & Guards 67 Pinnacle PCX Reagent Pump and ALKION™ Column for Aminoglycoside Catalog Information Valve Components Antibiotics, Paraquat/Diquat in Water 17 R eversed-Phase Columns & Guards 67 Vector PCX Reagent Pump Components Analysis & Streptomycin Catalog Information 67 PCX5200 Reagent Pumps with 58 Biogenic Amines/Polyamines Analysis 17 Specialty Columns Piston Wash 58 Muti-Residue Mycotoxin Analysis 18 Cation-exchange GARD™ Column 67 PCX5200 Reagent Pump Components Protection System APPLICATION KITS 67 Pinnacle PCX Replacement 18 Lithium Amino Acids 59 30-min High-Effi ciency Reactor Cartridge 25 Sodium Amino Acids Collagen Hydrolysate 68 Vector PCX Substitute Reactor 30 Carbamate Pesticide 59 30-min High-Effi ciency Protein Hydrolysate Volumes or Replacement Reators 34 Glyphosate Herbicide Analysis Column 59 Standard 60-min Protein Hydrolysate 68 PCX5200 Substitute Reactor 35 Alkion™ Column 59 High-Effi ciency 55-min Protein Hydrolysate Volumes or Replacement Reactor 39 Paralytic Shelfi sh Toxins 59 High-Effi ciency 55-min 68 Panels for PCX5200 40 Mycotox ™ Mycotoxin Collagen Hydrolysate 68 Reagent Reservoirs & Cap Assemblies 43 Polyether Antibiotics 59 60-min Oxidized Feed Hydrolysate 68 Pressure Regulators, Valves & Guages 44 Analysis of Voglibose in 60 70-min High-Effi ciency Physiologic 69 Flow Restrictors Pharmaceutical Formulations Fluid/Native Sample 69 Filters & Frits 45 Anion-exchange Column For Nitrite/ 60 Standard 185-min Physiologic 69 Tees & Unions Nitrate & Bromate Analysis Fluid/Native Sample 69 Tubings, Plastic 46 Analysis of Bromate in Drinking Water 60 High-Effi ciency 120-min Physiologic 70 Tubing, Type 316 Stainless Steel 47 Analysis of Alendronic Acid in Fluid/Native Sample 70 Fittings, Plastic Pharmaceutical Formulations 60 Temperature Gradient, Physiologic 70 Fittings, Type 316 Stainless Steel Fluid/Native Sample Kit 71 Fuses & Miscellaneous Accessories REAGENTS & CHEMICALS 60 High-Effi ciency 7-min PKU METHOD ABSTRACTS 49 TRIONE Ninhydrin Reagent Detection/Physiologic Fluid 50 o-Phthaladehyde 73 Afl atoxins (110) 61 Carbamate Pesticides, 50 Naphthalene-2,3-Dicarboxaldehyde 74 Multi-Residue Mycotoxin Analysis (203.1) 23+ Compounds 50 Vanillin 76 M ulti-Residue Mycotoxin Analysis 61 Carbamate Pesticides, EPA 51 p-Dimethylaminobenzaldehyde (DMAB) of Dry Grains (208) Method 531.1 51 Thiofl uor 78 Analysis of Alendronic Acid in 61 Carbamate Pesticides, AOAC 51 o-Phthalaldehyde Diluents Pharmaceutical Formulations (328) method 985.23 52 Solutions for Sample Preparation for 79 30-Min Amino Acid Analysis of 61 Carbamate Pesticides, AOAC Amino Acid Analysis (SERAPREP and Hydrolyzed Samples (380) method 531.2 URIPREP) 80 70-Min Amino Acid Analysis of 61 Glyphosate Herbicide, EPA Method 52 Hydrolysis Reagent for Carbamate Physiological Samples (382) 547, AOAC Method 991.08 Pesticide Analysis CB130 81 PKU and MSUD (107) 61 Biogenic Amines/Polyamines 52 Hydrolysis Reagent for Carbamate 82 Aminoglycoside Antibiotics in Feed (205) 61 Aminoglycoside Antibiotics Pesticide Analysis CB130.2 84 Analysis of Biogenic Amines (102) 61 Paraquat/Diquat in Water INTRODUCTION 86 Bromate Analysis (120) CUSTOMER SERVICE • If ordering a complete post-column 88 Post-column LC Analysis of & TECHNICAL SUPPORT system, please specify the brand 23 N-Methyl Carbamates (108) Pickering Laboratories has earned of HPLC with which it will be 90 Expanded HPLC Method for the reputation for strong Technical operating. We will advise you if N-Methyl Carbamates (112) Support in HPLC post-column tech- any modifi cations to the HPLC are 92 Analysis of Formaldehyde (325) nology: chemistry, columns, reagents, necessary. 93 Glyphosate and AMPA Analysis in and derivatization instruments. We Crops (206) From European Countries, Please start with assisting our customers in 95 Simultaneous Determination of Nitrite & Contact our European Distributor: identifying the best HPLC confi gura- Nitrate in Processed Foods (123) tion required for a successful integra- LCTech GmbH 98 Paralytic Shellfi sh Toxins (105.2) 100 Polyether Antibiotics (104) tion of our HPLC post-column sys- Postfach/P.O. Box 1360 101 Paraquat and Diquat in Water (117) tem. Then we continue our support D-84403 Dorfen, Germany 102 Sulfonamide Artifi cial Sweetners in through and beyond the warranty Telefon: +49(0)-80 81-93 68-0 Food Products (124) period to ensure our customers’ chro- Telefax: +49(0)-80 81-93 68-10 103 Analysis of Voglibose in Pharmaceutical matograms refl ect current standards email: [email protected] Formulations (327) of quality and reproducibility. Internet: www.lctech.de 106 General Terms and Conditions of Sale 107 Distributors HOW TO CONTACT US From non-European countries, please For Customer Service or Technical contact Pickering Laboratories directly. Support contact: We will provide the names of compa- nies which distribute our products. [email protected] [email protected] TERMS 800-654-3330 / 650-694-6700 03 • A minimum order is not required Fax: 650-968-0749 • Net 30 days for established ac- HOW TO PLACE AN ORDER counts, or prepayment in U.S. dol- Please contact us directly to lars, C.O.D., or Letter-of-Credit place an order: • We accept MasterCard & VISA, [email protected] Discover Card or American Ex- 800-654-3330 / 650-694-6700 press Fax: 650-968-0749 • Prices are Ex-Works Seller’s Fac- Pickering Laboratories, Inc. tory, Mountain View, California 1280 Space Park Way U.S. Destinations & Canada: Mountain View, CA 94043 Freight charges are prepaid and added to the invoice. Shipments are When ordering, please provide via UPS-Ground unless otherwise the following: specifi ed. • Complete shipping and billing Destinations Outside of the U.S.: addresses New Accounts: Before a credit-line • Catalog number and quantity has been established, new account of each item orders must be pre-paid. Freight • Brief product description, as found charges are collect. in Ordering Information Established Accounts in Good Standing: • Purchase order number (and con- Freight charges are collect. Unless oth- tract number, if applicable) erwise specifi ed, shipments overseas will be via consolidated air-freight to • Name and telephone number of keep the costs as low as possible. person calling Prices and specifi cations are subject • Confi rmation required to change without prior notice. • Special instructions, e.g., package mark- ings, mode of transit if other than UPS PRODUCT CATALOG 10/11 PICKERING LABORATORIES INTRODUCTION INSTALLATION & TRAINING FACTORY REPAIRS repairs potentially extending to the We encourage you to take advantage Pickering offers factory repair for it’s second day to allow for shipment of of Pickering Laboratories’ individu- instruments (PCX Model 5200, Pin- parts. The shipment of parts will be alized two or three-day installation nacle PCX and Vector PCX only). included in the invoice. and training courses. For an all-inclu- We will re-build or replace compo- On-site repairs costs will include sive fee we can provide post-column nents as necessary in our judgment, travel time, air fare, hotel, car rentals, system installation and training at then test the instrument to make expenses, plus parts and labor. your site by an experienced chemist certain that the original problem has from Pickering Laboratories or it’s been solved. COLUMN REPACKING SERVICE representatives. The training includes If you have determined or sus- For repairs, we will charge for shipping, performing analyses, routine main- pect that the performance of your parts and labor (current shop rate). tenance and troubleshooting, and Pickering cation-exchange column chromatogram interpretation. What is Required: or guard has degraded, consider our Obtain a Return Goods Authoriza- Repacking Service before purchasing Some of our authorized distributors tion Number (RGA) and a Health a new column. Columns eligible for offer their own training and installa- and Safety Declaration form. When repacking include the Pickering ami- tion services. the instrument is returned it must no acid, glyphosate and polyamine Whenever you buy installation and have the RGA noted on the outside columns and their respective guards. training by Pickering Laboratories, of the box and a completed Health please call Technical Support early and Safety Declaration, with the If you are not certain that the to schedule our visit to your site. This instrument. To expedite your repair column or guard must be repacked, is especially important if you buy we request the instrument is returned Pickering will test it for a service fee, installation through a distributor or with a purchase order for the cost to freight or postage prepaid. This fee HPLC Company because we need to evaluate the system (current shop rate). will be credited to any consequent repacking cost if tests indicate that hear all the details directly from you. What is Done: repacking is needed. Individually tailored courses are also Upon arrival we will evaluate your given at our facility in Mountain system to generate an estimated cost For this repacking service, Pickering View, California. Please telephone or for repairs. Once we have your ap- will charge 75 % of the current list fax with your requirements and we proval we will complete the repair. price of that particular column or will advise cost and schedule. We will replace damaged parts, pres- guard. If the column hardware can’t be sure test the instrument, and then re-used, Pickering Labs will provide a PRODUCT RETURNS test the instrument to confi rm the new column at 85 % of list price. Products can be returned if un- system meets the products original Repacked and tested columns and opened. A restocking fee of 15 % of manufacturer’s specifi cation. guards carry the same warranty as list price will be applied. Before return- What you get: new columns and guards. ing a product for any reason, please: The minimum parts replaced are: Analytical columns and guards will • Phone or fax requesting a Returned Restrictor(s), Seal(s) and Filters. If we be repacked only one time. Goods Authorization Number. test the instrument to determine that • Provide a Purchase Order Number it meets the original manufacturer’s LIMITED WARRANTY for product returns intended for specifi cation, we will warranty the Instruments instrument for 1 year on all the parts Pickering Laboratories, Inc., service and repair. that were replaced. If you request (Pickering) Instruments are warrant- • Package the product so that it will that we do not complete this test we ed to be free of defects in material arrive undamaged. Use the original will not warranty our work. and workmanship under normal packing if possible. If in doubt, installation, use, and maintenance, please call. Pickering Laboratories FIELD SERVICE for a period of one year from the cannot assume responsibility for Because of our limited personnel, we date of delivery to the customer. goods which arrive damaged in prefer to resolve problems without Pickering will replace or repair, shipment. site visits, but when that just is not without cost, any defective items. Ex- • Return the completed Health & enough, we will schedule a site visit pendable items such as check valves, Safety Declaration with the items with you. Please call for a quota- pistons, piston seals, and fi lters are intended for service or repair (See tion. On-site service entails an initial excluded from this warranty. In addi- page 4 for details.) evaluation on the fi rst day, with tion, physical damage, poor-quality INTRODUCTION reagent- and sample-induced dam- Pickering for examination or repair Laboratories. These offerings provide age, and instrument damage due to shall have Pickering’s prior approval factory trained engineers experi- customer’s misuse are not covered by (call for a Returned Goods Authori- enced in HPLC and post-column this warranty. zation Number) and be sent prepaid technologies to service your Pickering by the Customer. Return transporta- post-column derivatization instru- Analytical Columns tion will be at Pickering’s expense if ments. Each plan has the features and Pickering’s Analytical Columns are the Instrument, part of the Instru- benefi ts most requested by customers warranted for 90 days to be free of ment, or Analytical Column is found to offer the best value and effective- defects in materials and workmanship to be defective and under warranty. ness for today’s busy laboratories. under normal installation, use, and maintenance, for the warranted time HEALTH & SAFETY DECLARATION When you sign-up for the Pickering beginning from the date of delivery Instruments and columns must be Laboratories Service Plans you are to the customer. Pickering will replace decontaminated and cleaned prior assuring the continued quality and the Analytical Column under war- to returning to Pickering. To protect reliability of your post-column in- ranty if found defective in material or and ensure the safety of Pickering strument as well as your analysis. workmanship. However, the warranty personnel, customer must properly is void if the Analytical Column was BENEFITS OF SERVICE AGREEMENTS fl ush instrument of any and all haz- • Free Software Upgrades damaged due to customer’s misuse. ards (e.g. reactive chemicals, blood • Installation of all required performance How to Obtain Warranty Service borne pathogens). Please contact upgrades If there is a problem with your Pickering to obtain a Health and • PM/Service performed by Pickering Instrument or Analytical Column Safety Declaration Form (B-0107) Laboratory trained service specialists within the warranty period, im- describing return instructions and • Fixed Annual Cost – No Surprises mediately notify Pickering or your customer responsibilities. Failure to • Unlimited Technical/Analytical Support 05 local authorized representative. If do so will result in delay of service the Instrument or Analytical Col- and/or additional charges. • Multi-Unit Service Discounts Available umn was not purchased directly INSTRUMENT & SERVICE PLANS from Pickering, please contact the Introducing the Service Plan to vendor where it was purchased. Any match the quality and reliability you Instrument, part of the Instrument, have come to expect from Pickering or Analytical Column returned to COMPARISON OF SERVICE PLANS FEATURES FACTORY WARRANTY WARRANTY UPGRADE DEPOT SERVICE ELITE SERVICE* Telephone support to isolate Monday - Friday Monday - Friday Monday - Friday Monday - Friday and resolve technical problems 8AM - 5PM 8AM - 5PM 8AM - 5PM 8AM - 5PM Excluding Holidays Excluding Holidays Excluding Holidays Excluding Holidays Recertifi cation Not included Included Included Included Site visits At prevailing rates Two (including PM) At prevailing rates Two (including PM) Preventative Maintenance At prevailing rates One One One Freight Charges Not Included Included Included Included Parts and Labor Included Included Included Included Service Plans offered for the Pickering Laboratories PCX5200, Pinnacle PCX and Vector PCX post-column derivatization instruments. Pre-service inspection is required for instruments that have an expired manufacturers warranty or service contracts. *The Service plan provides a total of two service calls that will cover parts, labor and travel for the period of one year. Included is unlimited telephone support from Pickering Technical Service Engineers and Applications Specialists. For more information and quotations, please contact Pickering Laboratories’ headquarters Technical Support at [email protected] or 800-654-3330. PRODUCT CATALOG 10/11 PICKERING LABORATORIES “A LOT HAS CHANGED SINCE PRODUCING OUR FIRST HPLC POST-COLUMN DERIVATIZATION SYSTEM, BUT OUR GOALS HAVE REMAINED THE SAME; PROVIDE TOP-NOTCH SYSTEMS WITH THE GREATEST FLEXIBILITY AND QUALITY.” We have optimized our instruments for post-column applications as well as ease of use, ruggedness and efficiency. MICHAEL V. PICKERING, PH.D. / FOUNDER SAREETA NERKAR / RESEARCH CHEMIST Chalk talk: giving words vision. INSTRUMENTS & HARDWARE 07 PINNACLE PCX DERIVATIZATION INSTRUMENT Part of the complete integrated system of instruments, chemicals, columns, methods and support from Pickering Laboratories. The Pinnacle PCX Sigma Series is an optimized HPLC post-column derivatization system for analysis of Amino Acids, Carbamates, Mycotoxins, Antibiotics and many other applications. This instrument is the culmination of Pickering Laboratories’ 30 years of experience in post-column analysis instrument manufacturing. Each component is specifi cally designed to optimize the sensitivity and selectivity of post-column analysis. Only Pickering Laboratories offers the complete package of chemicals, columns, methods and post-column systems. Because each part of the method is designed to work together, Pickering Laboratories can offer the extraordinary promise that the analysis is guaranteed to work for the intended application. The Pinnacle PCX refl ects the ease of use, reliability and ruggedness you have come to expect from Pickering Laboratories. SYSTEM DESIGN ADVANCEMENTS RESULT IN OPTIMIZED ANALYSIS: • The electronic syringe pump provides true pulse-free fl ow for superior sensitivity and consistency. The pump cylinder and head is made from a single piece of inert ceramic for durability and non-reactivity. • Electronic valves eliminate troublesome check valves and allow automated pump fl ushing. • The quick-change reactor cartridge makes application switching easy and replacements quick and inexpensive. • The column oven utilizes circulating air for consistency of heating and quick cooling. • Inert fl ow path extends system life and reduces maintenance. • The PCX control software allows for precise control of the reagent delivery and conservation. • Column oven temperature gradient programming improves separation and run times. Pinnacle PCX is the only post-column system with this feature. • Works with any HPLC system. • RoHS compliant PRODUCT CATALOG 10/11 PICKERING LABORATORIES INSTRUMENTS & HARDWARE FEATURE HIGHLIGHT The Pinnacle PCX has a column oven capable of programmable temperature gradients. This feature is useful in Amino Acid Analysis by enabling additional fl exibility when optimizing methods. Using the temperature gradient program allows for improved separation, shortened analysis time and fi ne-tuned methods for specifi c compound detection. The Chromatogram in Figure 1 demonstrates some of the clear advantages of the temperature gradient program. Com- pared to the standard method without the temperature gradient the total run time is 42 % shorter. Additionally, separation is improved. With the temperature gradient program, you are able to focus on different areas of the chromatogram for your specifi c compound of interest. 1 FIGURE 1 High-effi ciency Lithium 20 23 for Physiological samples using temperature 15 17 45 gradient 16 70 o C 18 21 65 o C 36 24 2 5 78 11 19 25 30 37 39 44 10 22 26 27 35 38 40 3 9 13 32 34 43 31 34 o C 14 : 28 29 33 41 42 4 6 12 0 10 20 30 40 50 60 70 min 1 Phosphoserine 24 Isoleucine PINNACLE PCX SPECIFICATIONS 2 Taurine 25 Leucine • Dimensions: (h x w x d): 54 x 26.7 x 46.4 cm 3 Phosphoethanolamine 26 Tyrosine 4 Urea 27 Phenylalanine (21.25 x 10.5 x 18.25 inches) 5 Aspartic acid 28 β-Alanine • Weight: 60 lbs (27.2 Kg) – Duplex 6 Hydroxyproline 29 β-Amino-i-butyric acid • Electrical: 100-120 V, 50/60 Hz, 1.7 A, 200 W or 200-240 V, 7 Threonine 30 Homocystine 50/60 Hz, 0.8 A, 200 W 8 Serine 31 γ-Aminobutyric acid 9 Asparagine 32 Tryptophan • Mains voltage ± 10 % of nominal 10 Glutamic acid 33 Ethanolamine • Installation (over voltage) category II, pollution degree 2 11 Glutamine 34 Ammonia • Indoor use only 12 Sarcosine 35 Hydroxylysines 13 α-Aminoadipic acid 36 Ornitine • Environmental: Altitude up to 6500 ft 14 Proline 37 Lysine • Ambient Temperature 15 °- 25 °C 15 Glycine 38 1-Methylhistidine 16 Alanine 39 Histidine • Relative humidity up to 80 % at 31 °C 17 Citrulline 40 3-Methylhistidine REAGENT PUMPS 18 α-Amino-n-butyric acid 41 Anserine • True pulse-free syringe pump 19 Valine 42 Carnosine 20 Cystine 43 Arginine • Single piece ceramic barrel 21 Methionine 44 Glucosaminic Acid* • Completely inert fl ow path 22 Allo-Isoleucine 45 2-Aminoethyl-cysteine (AEC)* • Maximum operating pressure 500 psi 23 Cystathionine • Programmable fl ow rate *Internal Standard