PLANT MITOCHONDRIA Structural, Functional, and Physiological Aspects PLANT MITOCHONDRIA Structural, Functional, and Physiological Aspects Edited by A. L. Moore University of Sussex Brighton, England and R. B. Beechey University College of Wales Aberystwyth, Wales SPRINGER SCIENCE+BUSINESS MEDIA, LLC Library of Congress Cataloging in Publication Data International Meeting on Plant Mitochondria (2nd: 1986: Aberystwyth, Dyfed) Plant mitochondria. "Proceedings of the Second International Meeting on Plant Mitochondria. . . held July 20-24, 1986, in Aberystwyth, Wales"-T.p. verso. Bibliography: p. Includes index. 1. Plant mitochondria —Congresses. I. Moore, A. L. (Anthony L.), date. II. Beechey, R. B. (R. Brian) III. Title. QK725.I4946 1986 581.87'342 87-15303 ISBN 978-1-4899-3519-9 ISBN 978-1-4899-3519-9 ISBN 978-1-4899-3517-5 (eBook) DOI 10.1007/978-1-4899-3517-5 Proceedings of the Second International Meeting on Plant Mitochondria: Structural, Functional, and Physiological Aspects, held July 20-24, 1986, in Aberystwyth, Wales © 1987 Springer Science+Business Media New York Originally published by Plenum Press, New York in 1987 Softcover reprint of the hardcover 1st edition 1987 All rights reserved No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the Publisher PREFACE Eight years have elapsed since the first International Meeting on Plant Mitochondria was held in Marseilles. Since this date numerous important developments have occurred within the field and hence a further conference on this fundamental area of research was considered well overdue. This volume summarises the lecture and poster sessions of the second International Meeting on Plant Mitochondria held in Aberystwyth, July 20- 24th, 1986. The meeting was held not only to bring together plant scientists interested in the bioenergetics of plant mitochondria but also those who are interested in the regulatory role of mitochondria in plant growth and respiration. A further important aspect of this conference was to introduce plant physiologists and biochemists to the plant molecular biologists in an attempt to not only discuss problems of mutual interest but to also learn much more about the real questions which the biochemists and physiologists wish to answer. Hopefully the volume reflects much of the current excitement and advances being made in the field. Although many of the participants of the first meeting were present the expertise of Walter Bonner, Jack Hanson and Gaston Ducet, to name but a few, was sorely missed. The conference consisted of forty-five minute review lectures followed by thirty minute research lectures, the summaries of which are found in the longer articles. The meeting was divided into four seSSions, namely, organisation of the electron transport chain; mitochondrial interactions; mitochondrial biogenesis and plant growth and development. The poster abstracts have been arranged in such a manner that they are associated with each of the four sessions. We gratefully acknowledge the finanCial support recieved from Bio-Rad Laboratories Ltd., Ciba-Geigy Agrichemicals, Du Pont (UK) Ltd., Flow Laboratories Ltd., Gelman Sciences Ltd., GIBCO-BRL., Hansatech Ltd., Millipore (UK) Ltd., Severn Science Ltd., Shell Research Ltd., Unilever Research & Engineering Division and Wellcome Research Laboratories. We are also extremely grateful to Mike Proudlove, Alyson Tobin, Naheed Kaderbhai, Mike Brailsford, Mike Walsh, Simon Dobbs, Andy Thompson and Mike Jones for their help with the day-to-day conference running. The help and enthusiasm of the Advisory Panel, conSisting of Tadashi Asahi, David Day, Ingemar Ericson, Hans Heldt, Hans Lambers, Claude Lance, Chris Leaver, Don Moreland, John Palmer, Anna Rychter, Jim Siedow and last but not least the instigator of this conference Joe Wiskich, is also gratefully acknowledged. We thoroughly enjoyed organising the meeting and look forward with eager anticipation to the next conference, to be organised by Hans Lambers, in the not too distant future. Tony Moore Brian Beechey 1986 v CONTENTS ORGANISATION OF THE ELECTRON TRANSPORT CHAIN General Organization of the Respiratory Chain and Matrix Associated Specific Dehydrogenases in Higher Plant Mitochondria 1 R. Douce and M. Neuburger Relationships Between Membrane Potential and Oxidation Rate in Potato Mitochondria 17 P. Diolez and F. Moreau The Role of Quinone Pools in Regulating Electron Transport in Plant Mitochondria 27 C.J. Kay and J.M. Palmer The Nature of the Terminal Oxidation Step of the Alternative Electron Transport Pathway 37 P. Rustin The Ubiquinol/Cytochrome c Oxidoreductase Complex of Plant Mitochondria and its Interaction with the Alternative Cyanide Resistant Oxidase: A Membrane Complementation Study 47 C. Spinelli and D. lannoni Regulation of ADP-Limited Respiration in Isolated Plant Mitochondria 59 D.A. Day. J.T. Wiskich. J.H. Bryce and I.B. Dry Ohmicity. Proticity and Electron Flux in Mung Bean Mitochondria 67 S.T. Dobbs and A.L. Moore Competition Between Malic Enzyme and Malate Dehydrogenase in Isolated Plant Mitochondria 73 J.H. Bryce and J.T. Wiskich Effects of Adenine Derivatives on Permeability and Dehydrogenase Activities of Potato Mitochondria 77 M. Chaveau. I. Funes and J. Roussaux Kinetics of Electron Transport in Complex III of Plant Mitochondria During Ageing in vitro 81 A-C. Fricaud and J. Dupont The Effects of Inhibitors of the b-c1 Complex on the Respiration of Mitochondria from Aged Potato Discs 85 J.D. and A.M. Tenreiro Arraba~a The Redox State of Mitochondrial Pyridine Nucleotides Versus Rate of Substrate Oxidation 89 P. Rustin, M. Neuburger, R. Douce and C. Lance Effects of Inhibitors on the Membrane Potential (6~) of Mung Bean Mi tochondri a 93 D.E. Moreland and W.P. Novitzky Effect of the Antimalarial Quinone BW 58C on Cyanide-Sensitive and Cyanide-Insensitive Plant Respiration 97 D.C. Holt, J.B. Hyndam and R. Cammack Redox Properties of Inhibitors of the Alternative Pathway of Plant Mitochondria 101 J. Dupont and P. Rustin Hydroxamate-Activated Peroxidases: Interference with Determination of Respiratory Pathway Activity? 105 L.H.W. van der Plas, H. Gude and M. Wagner Cytokinins and the Inducement of the Cyanide-Resistant Alternative Pathway 109 A. Hourmant and P. Dizengremal A Determination of the Molecular Weight of the Aroid Alternative Oxidase by Radiation Inactivation Analysis 113 D.A. Berthold, D.J. Fluke and J.N. Siedow Partial Purification, Reconstitution and Antibody Production to the Alternative Oxidase of Sauromatum guttatum Mitochondria 117 T.E. Elthon and L. McIntosh Immunological and Electrophoretic Analysis of the Alternative Oxidase of Arum maculatum Sub-Mitochondrial Particles 121 M.C. Walsh and A.L. Moore The Effects of Different Ionic-Conditions on the Activity of Cytochrome c-Oxidase in Purified Plant Mitochondria 127 B. Wigge and P. Gardestrom Purification of Jerusalem Artichoke Mitochondria on a Continuous Percoll Gradient 131 I.M. M~ller and A. Liden Generation and Characterization of Sub-Mitochondrial Particles: Isoelectric Points and Charge Surface Density of Plant Mitochondrial Membranes 135 P. Petit, K. Edman, P. Gardestrom and I. Ericson Lateral Heterogeneity in the Inner Mitochondrial Membrane 139 A. Liden, M. Somrnarin and I.M. M~ller Reconstitution of Oxidative Phosphorylation in Pea Cotyledon Submitochondrial Particles 143 A. Horak, H. Horak and M. Packer A Plant Mitochondrial ATPase/Synthase 147 J. O'Rourke and S.B. Wilson viii MITOCHONDRIAL INTERACTIONS Interactions of Mitochondria with Other Metabolic Processes - an Overview 151 I.B. Dry and J.T. Wiskich The Effect of light on Mitochondrial Respiration Studied by Rapid Fractionation of Protoplasts 161 P. Gardestrom On the Function of Malate-Oxaloacetate Shuttles in a Plant Cell 171 H. Ebbighausen, M.D. Hatch, R.McC. lilley, S. Kromer, M. Stitt and H.W. Heldt Mechanism and Control of Mitochondrial Glycine Oxidation 181 D.J. Oliver and J.l. Walker Higher Plant Mitochondrial pyruvate Dehydrogenase Complexes 189 J.A. Miernyk, B.J. Rapp, N.R. David and D.O. Randall In vitro Studies on the Interactions in Malate Metabolism Between Mitochondria and Chloroplasts from CAM-Performing Kalanchoe blossfeldiana 199 V. Adam and P. Rustin Regulation of Glycollate Metabolism in Mitochondria Isolated from Euglena gracillis Klebs Strain z Pringsheim 203 M.J. Merrett A Comparison of Mitochondria from Soybean Nodules, Roots and Cotyledons 207 D.A. Day, G.D. Price and P.M. Gresshoff Respiratory Participation by During Early Mitochondri~ Plastidogenesis in Barley Seedlings 211 J.H. Owen and A.R. Wellburn Co-Ordinated Organelle Biogenesis During Photorespiratory Development in Wheat leaves 215 A.K. Tobin, N. Sumar, M. Patel, M.O. Proudlove, G.R. Stewart and A.l. Moore Glycine Uptake by Pea leaf Mitochondria: A Proposed Model for the Mechanism of Glycine - Serine Exchange 219 D.J. Oliver Some Properties of Plant Mitochondrial pyruvate Dehydrogenase Kinases 223 J.A. Miernyk and D.D. Randall The Uptake and the Oxidation of Pyruvic Acid by Castor Bean Mitochondria 227 R.B. Beechey, M.A. Brailsford and A.G. Thompson pyruvate Transport by Thermogenic Tissue Mitochondria 233 M.O. Proud love, R.B. Beechey and A.l. Moore An Enzyme Profile of Isolated Plant Mitochondria 239 K.l. Soole, I.B. Dry and J.T. Wiskich Another Type of Alternative Electron Transfer Pathway in the Yeast ~ P.~Rsilosis 243 M. Guerin, N. Camougrand, A. Cheyrou and M-F. Henry Evidence of a Positive Membrane Potential and a Calcium Accumulation in z Mitochondria ~glen& Gracil~ by in vivo Microelectrode Studies 247 C. Tamponnet, J-P. Rona, J-N. Barbotin and R. Calvayrac MITOCHONDRIAL BIOGENESIS Structure and Biogenesis of the Plant Mitochondrial Inner Membrane 251 M.J. Hawkesford and C.J. Leaver Synthesis of the Nuclear DNA-Encoded Subunits of Higher Plant Cytochrome c Oxidase and F ATPase 265 T. Asahi, M. Maeshima, T. Nakagawa,1K• Kobayashi Y. Iwasaki and K. Nakamura Two Nuclear Genes Encoding the Beta Subunit of the IYJitochondrial ATP Synthase in Nicotiana plYJnbJ}ginfolia 275 ,>1. Boutry Divergence in mtDNA and Effects in Interspecific Combinations of Nuclear and Mitochondrial Genomes in the Yeast Genus 283 ~~Q!llyces M. Claisse, F. Michel and D. Hawthorne A Comparative Transcriptional Analysis of the Coding Regions in the CMS-T Unique Reading Frame between Fertile and T Male Sterile Cytoplasm 293 J. Oin, N. Walker, C. Fauron, D. Gupta and A.G. Abbott Cytochrome Oxidase from Etiolated Corn Seedlings 301 R. Dutch, R. Ingle, J. Centeno, W. Peiffer, G. Babcock, and S. Feryuson-l>1i ller Dicyclohexylcarbodiimide Protects Against Helminthosporium maydis Race T Toxin Action on Susceptible Corn Mitochondria 305 M.J. Holden and H. Sze Castor Bean Mitochondrial Protein Synthesis in Response to Temperature Induced Stress 309 N. Kaderbhai. R.B. Beechey and M. Kaderbhai Citrate Synthase Messenger RNA in Relation to Enzyme Synthesis in Euglena 313 J.E. Parker Protein Synthesis. Respiration and Growth in EugJena z in the Presence of Antimycin A 317 gracil~ P. Benichou. R. Calvayrac and M.L. Claisse PLANT GROWTH AND RESPIRATION Respiration in Intact Tissues: Problems and Perspectives 321 H. Lambers and D.A. Day x On the Intergration of Plant Growth and Respiration 331 R. de Visser Fructose-2,6-Bisphosphate and Plant Respiration 341 T. ap Rees and J.E. Dancer The Significance of Cyanide-Resistant Respiration to Carbohydrate Metabolism in Higher Plants 351 J.N. Siedow and M. E. Musgrave Nature of the Terminal Respiratory Oxidases in Sweet Potato Slices 361 T. Solomos The Alternative Oxidase in Barley Roots of Differing Growth and Respiration Rates 365 I.J. Bingham and J.F. Farrar The Participation of Cyanide Resistant Pathway in Respiration of Winter Rape Plants Treated with Low Temperature 369 A.M. Rychter and E. Cie~la NADPH Oxidation in Potato Tuber Callus Mitochondria and its Physiological Significance During in vivo Respiration 373 A.M. Wagner, D-J. Leek and L.H.W. van der Plas The Role of some Phytohormones in the Regulation of Respiratory Pathways in Potato Tuber Tissue 377 H. Gude, P. van't Padje, E. Rietveld and L.H.W. van der Plas Ageing of Carrot Slices; Respiration of Discs and Mitochondria Obtained from them as Compared with Fresh 381 V. Vaz-Pinto Relationship oetween Structural Mitochondrial Parameters and Respiration Rates in Variegated Leaves of Dieffenbachia 'CAI"ILLA' 385 J.L. Araus, R. Calafell, G. Burgos, J.F. Aguila and J. Azc6n-Bieto The Effect of Temperature on Respiratory Pathways of Fatsia Leaves 389 j~p.onica G. Burgos, J.L. Araus and J. Azc6n-Bieto Salt Tolorance - Does Leaf Respiration Have a Contribution to Make? 393 S. Brown, D.A. Day and C. Critchley Effect of Salt Stress on Properties of Mitochondria Isolated From Etiolated and Greening Maize Seedlings 397 Y. Jolivet and P. Dizengremel Optical Properties and Respiratory Activity in the Marine Dinoflagellate cohnii 401 ~pthecodinium_ M. Denis, T. Packard and M. Rodier Nitrate Reductase Activity in'Seeds and Seedlings of Tropical Species 405 A.M. Udoh, A.K. Tobin, M.O. Proudlove and A.L. Moore Contributors 409 Index 411 xi