Non-Invasive Assessment of Right Ventricular Function in Health and in Acute and Chronic Pulmonary Arterial Hypertension Shareen Jaijee A thesis submitted in fulfilment of the requirements for the degree of Doctor of Philosophy Sydney Medical School University of Sydney Australia 2017 1 Declaration This is to certify that to the best of my knowledge, the content of this thesis is my own work. This thesis has not been submitted for any degree or other purposes. Two semesters of this work were done at The University of Sydney, as well as much of the preparatory work for the results of all of the chapters. I certify that the intellectual content of this thesis is the project of my own work and that all the assistance received in preparing this thesis and sources have been acknowledged. Signature: Name: Shareen Jaijee 2 Abstract This thesis investigates the utility of cardiac magnetic resonance imaging (CMR) to assess right ventricular function and dynamic right ventricular (RV) reserve, in health and in acute and chronic pulmonary hypertension. Right ventricular function is a strong predictor of prognosis, in patients with pulmonary arterial hypertension (PAH), but there are currently no available clinical tests that can predict which right ventricle is destined to fail, despite medical therapy. RV reserve, or the ability of the RV to augment during exercise, has recently gained more attention as a potential marker vascular- ventricular uncoupling, and CMR potentially offers a novel, accurate and reproducible method to assess this. Exercise CMR is in its developmental stages, and while several groups have used it in healthy volunteers and patients to study dynamic biventricular function, approaches have varied. We set out to develop a novel and robust exercise CMR methodology and demonstrate its accuracy and reproducibility. Furthermore, we aimed to show how timing of acquisition and respiratory variation, are important considerations, potentially affect key pathophysiological changes. In Chapter 3, we outline the developmental considerations and demonstrate intra-observer, inter- observer, inter-study and inter-test reproducibility. In Chapter 4, we show how the RV remodels after pulmonary thromboendarterectomy in patients with chronic thromboembolic pulmonary hypertension, in a retrospective analysis of CMRs acquired before and after surgery. Importantly, we demonstrate how biventricular interactions, expressed as a composite measure of RV end diastolic volume to left ventricular (LV) end diastolic volume ratio, correlates with change in 6MWD, but parameters of each 3 ventricular alone do not. Furthermore, we demonstrate a correlation between left atrial size and left ventricular end diastolic index, showing that under-filling is likely to be an important pathophysiological explanation of a reduction in LV size, in these patients. LV under-filling from increased RV pressure, and biventricular interactions, continue to remain key themes throughout the following experiments. While exercise CMR is a new technique, so is assessment of RV reserve and there are no normal values available. Furthermore, there is conflicting evidence in the literature, as to normal cardiovascular changes that occur during exercise in normal hearts. We demonstrate in Chapter 5 that an increase in RV forward flow is due to a decrease in RV end systolic volume and an increase in RV ejection fraction, leading to an increase in LV end diastolic volume. LV ejection fraction increases as a result of an increase in left ventricular end diastolic volume and a decrease in left ventricular end systolic volume. Understanding normal exercise physiology, during continuous steady state exercise, is key to interpreting pathophysiological changes. Acute normobaric hypoxia causes hypoxic pulmonary vasoconstriction and an increase in pulmonary vascular resistance and pulmonary pressures. It offers a model in which RV reserve in healthy controls can be studied. It has been hypothesised that the reduction in VO2 max in acute hypoxic exercise is a consequence of a reduction in RV forward flow, however this has never been definitively documented in an imaging study. We show, for the first time using exercise CMR in Chapter 6, that exercise during acute hypoxia leads to a reduction in RV forward flow, a blunting of the rise in RV ejection fraction and LV under-filling with a reduction in LV forward flow. We also show that there is a blunting of the rise in MPA average blood velocity on exercise during acute hypoxia, and hypothesise that this could be a novel CMR parameter to assess pulmonary vascular distensibility. 4 Identifying patients whose RV will continue to fail despite medical therapy has remained elusive. We go on to demonstrate in Chapter 7 that our approach to exercise CMR is not only feasible in patients with pulmonary arterial hypertensoin, but that exercise in these patients, considered stable on medical therapy with normal resting RV ejection fraction, unmasks impaired right ventricular reserve. Furthermore, we demonstrate that there is heterogeneity of response that cannot be predicted by routine clinical tests and resting biventricular function. This information could potentially be clinically valuable, and we outline where this research will take us to next in Chapter 8, where we hope to demonstrate change in right ventricular reserve before and after medical therapy, in patients with inoperable chronic thromboembolic pulmonary hypertension, predicts long term clinical outcomes on follow up. Together, these studies demonstrate how CMR can accurately and non-invasively assess RV remodeling and RV reserve, its impact on the LV, and unmask right ventricular – vascular uncoupling which is otherwise not present at rest, in patients with acute and/or chronic pulmonary arterial hypertension. 5 Acknowledgements Firstly to the healthy volunteers and patients who enthusiastically and generously gave their time to participate in this study, I am eternally grateful. To my supervisors at Sydney University, Professor David Celermajer and Dr Raj Puranik – thank you for your ongoing support, insight and advice throughout this process. To my supervisors at Hammersmith Hospital, Imperial College London, Dr Declan O’Regan and Dr Simon Gibbs – I cannot express my gratitude enough, for the trust you put in me, and also for your unwaivering support. I have learnt so much from you both as a clinician and researcher. Thanks must go to Dr Fiona Kermeen and her team for inviting us to collaborate on research with her. I am also grateful to Dr Andre LaGerche and Dr Guido Claessen, who so openly and graciously allowed me to see their exercise CMR set up in Leuven, as well as supporting my pursuits in the same research area. Thanks also to those who provided essential technical assistance, including Dr Matthew Clemence from Philips, who helped develop the real time cine acquisitions and Dr Luke Howard for his exercise physiology expertise. I would also like to acknowledge the financial support given to me by the British Heart Foundation, the Medical Research Council and the Sydney University Post Graduate Research support scheme. I have had the great honour and pleasure of meeting and developing firm friendships, with the most fantastic, enthusiastic and dedicated team in the Robert-Steiner Unit and the Pulmonary Hypertension Unit at Hammersmith Hospital, whose input in to this project has been essential. Thanks must go to Radiographers, Mr Ben Statton, Ms Marina Quinlan and Ms Alaine Berry, 6 Research nurses, Ms Tamara Diamond and Ms Laura Monje-Garcia, Pulmonary Hypertension nurses Ms Wendy Gin-Sing and Ms Eilish Lawlee, Physicist Dr Pawel Tokarczuk, Exercise Laboratory technicians Ms Hannah Tighe and Dr Kevin Murphy and Clinical Lecturer, Dr Antonio De-Marvao. All of you took on this project as if it was your own and I am eternally grateful for your hard work. Many of you provided important insights and suggestions, and worked unsociable hours to ensure research subjects were accommodated. Thanks also to Dr Tim Dawes, Dr Punam Pubari and Dr Mark Attard for your friendship through this process. Most importantly, I want to thank my family. To both my maternal and paternal grandparents whose entire mission in life was to ensure their children had an education, despite their own circumstances – you are constantly an inspiration in life. To my parents, Drs Harpal Kaur and Harbans Singh Gill who are the most honest and hard-working people I know, who have dedicated your lives to ensuring that my brother Davinder and I, have had everything in life that you both never had. You are examples of how kindness, community spirit, humility and hard-work will get you far in life. I am eternally grateful to my late mother-in-law Mrs Mohinderpal Kaur Jaijee, my father-in-law, Dr Daljit Singh Jaijee and my friend Mrs Manpreet Kaur Bassi, who have provided endless support and childcare. To my husband Anoop, who is my greatest support in everything that I do and has spurned me on despite whatever barrier has been thrown in my way, you are my best friend and an amazing husband. This thesis would have been impossible without you. Finally to my beautiful son Ashwin – you make writing a thesis difficult, but your endless hugs, kisses and laughter make life heavenly. 7 Presentations Arising from this Thesis Right Ventricular Function in Acute and Chronic Pulmonary Hypertension using Exercise Cardiac Magnetic Resonance Imaging – European Society of Cardiology, August 2016 Deterioration of Right Ventricular Function on Exercise detected by Exercise Cardiac Magnetic Resonance Imaging in Patients with Pulmonary Arterial Hypertension – British Cardiovascular Society Meeting, June 2016 Cardiac Magnetic Resonance Imaging in Healthy Volunteers in Normoxic and Hypoxic Exercise – Society of Cardiovascular Magnetic Resonance Imaging Meeting, January 2016 Exercise Cardiac Magnetic Resonance Imaging – Medical Research Council ‘University of the Third Age’ event London, United Kingdom, January 2016 ‘Exercise Cardiac Magnetic Resonance Imaging’ Pulmonary Hypertension Physicians Research Forum, London, United Kingdom, November 2015 Manuscripts in Preparation Arising from this Thesis to Date Shareen Jaijee, Marina Quinlan, Pawel Tokarczuk, Matthew Clemence, Luke Howard, Simon R Gibbs, Declan P O’Regan, Right Ventricular Reserve in Acute and Chronic Pulmonary Hypertension (Submitted) 8 Shareen Jaijee, Marina Quinlan, Ben Statton, Pawel Tokarczuk, Matthew Clemence, Luke Howard, Simon R Gibbsm Declan O’Regan, Exercise Cardiovascular Magnetic Resonance imaging and the Timing of Image Acquisition: A Key Player in Detecting Pathophysiological Changes Shareen Jaijee, Marina Quinlan, Ben Statton, Pawel Tokarczuk, Matthew Clemence, Luke Howard, Simon R Gibbsm Declan O’Regan, Recreational exercise and the effect of Resting and Dynamic Biventricular Structure and Function Awards and Grants Arising from this Thesis Australian Postgraduate Award Scholarship Postgraduate Research Scholarship British Heart Foundation Project Grant 9 Table of Contents Declaration ......................................................................................................................................... 2 Abstract .............................................................................................................................................. 3 Acknowledgements ........................................................................................................................... 6 Presentations Arising from this Thesis ........................................................................................ 8 Manuscripts in Preparation Arising from this Thesis to Date ............................................... 8 Awards and Grants Arising from this Thesis ............................................................................. 9 List of Figures ................................................................................................................................. 14 List of Tables .................................................................................................................................. 18 Abbreviations ................................................................................................................................. 21 Chapter 1 - Introduction .............................................................................................................. 25 1.1 Right Ventricular Structure and Function .............................................................................. 25 1.1.1 Right Ventricular Anatomy ...................................................................................................................... 25 1.1.2 Myofibres and RV Contraction ............................................................................................................... 28 1.1.3 Ventricular Interaction ............................................................................................................................... 28 1.1.4 Physiology of the Right Ventricle .......................................................................................................... 29 1.2 Normal exercise physiology ............................................................................................................. 30 1.3 Assessment of the Right Ventricle ................................................................................................. 32 1.4 Acute Hypoxia .................................................................................................................................... 38 1.5 Pulmonary Hypertension ................................................................................................................. 39 1.5.1 Chronic Pulmonary Arterial Hypertension .......................................................................................... 40 1.5.2 Chronic Thromboembolic Pulmonary Hypertension ....................................................................... 40 1.5.3 Consequences of Pulmonary Arterial Hypertension to the Right Ventricle ............................. 41 1.5.4 Exercise in the Assessment of Pulmonary Arterial Hypertension ............................................... 42 1.6 Cardiovascular Magnetic Resonance Imaging ........................................................................... 46 1.6.1 Cardiovascular Magnetic Resonance Imaging Physics ................................................................... 46 1.6.2 Two dimensional phase-contrast imaging ........................................................................................... 50 1.6.3 Real time MRI ............................................................................................................................................... 51 1.6.4 Exercise MRI ................................................................................................................................................. 54 1.7 Summary, Hypotheses and Specific Aims .................................................................................... 56 Chapter 2 – General Methodology ............................................................................................. 59 2.1 Ethics Approval ................................................................................................................................. 59 2.2 Funding ................................................................................................................................................ 59 2.3 Study population and Recruitment for the Exercise CMR study ........................................... 59 2.3.1 Healthy Volunteers ...................................................................................................................................... 60 2.3.2 Pulmonary Arterial Hypertension Subjects ......................................................................................... 61 2.4 Recruitment ........................................................................................................................................ 62 2.4.1 Healthy volunteers ....................................................................................................................................... 62 2.4.2 Participants with Pulmonary Arterial Hypertension ........................................................................ 62 2.5 Follow up ............................................................................................................................................. 62 2.6 Our Services ........................................................................................................................................ 62 2.7 Statistical Analysis: ........................................................................................................................... 63 2.8 Clinical Data ....................................................................................................................................... 63 2.8.1 6MWD ............................................................................................................................................................. 63 2.8.2 Cardiopulmonary Exercise Testing ........................................................................................................ 64 2.8.3 Lung Function Testing ............................................................................................................................... 64 10