Zwick, P. & R.W. Baumann. 2011. Nemoura jejudoensis a new species of stonefly and the redescription of Amphinemura baei Ham and Lee (Plecoptera, Nemouridae) from Jeju Island, Korea. Illiesia, 7(15):148-155. Available online: http://www2.pms-lj.si/illiesia/papers/Illiesia07-15.pdf NEMOURA JEJUDOENSIS A NEW SPECIES OF STONEFLY AND THE REDESCRIPTION OF AMPHINEMURA BAEI HAM AND LEE (PLECOPTERA, NEMOURIDAE) FROM JEJU ISLAND, KOREA Peter Zwick1 and Richard W. Baumann2 1 Schwarzer Stock 9, D-36110 Schlitz, Germany E-mail: [email protected] 2 Department of Biology and Monte L. Bean Life Science Museum, Brigham Young University, Provo, UT, U.S.A. 84602 E-mail: [email protected] ABSTRACT Nemoura jejudoensis sp. n. is described from specimens collected on Jeju Island, Korea. A detailed redescription is given of Amphinemura baei Ham and Lee 1999, including new illustrations and scanning electron micrographs of adult genitalia based on numerous specimens collected from Jeju Island. In addition, a list of miscellaneous stonefly species from Korea is included based on specimens housed in the Monte L. Bean Life Science Museum, Brigham Young University, Provo, Utah USA. Keywords: Plecoptera, Nemouridae, Amphinemura, Nemoura, new species, stoneflies, Jeju Island [= Cheju-do], Korea INTRODUCTION localities. Our paper also includes a list of the A paper on the stonefly fauna of Korea based on a stoneflies from Korea that are presently kept in the large recent malaise trap collection of stoneflies Brigham Young University collection. possessed by the Smithsonian Institution, Washington, D.C. (Zwick 2010) engendered new MATERIALS AND METHODS interest in the Korean stonefly fauna. It brought to Specimens were studied using Wild M-8 stereo mind a small collection of stonefly adults that were microscopes. The Scanning Electron Micrographs collected in the 1980s from several waterfalls on Jeju were produced at Brigham Young University using a Island [= Cheju-do], that had not been studied Philips XL30 ESEM FEG electron microscope. Line sufficiently. The present paper is based on these drawing illustrations were produced using a Leica specimens that were obtained by Michael Whiting DMLS microscope in Schlitz, Germany. Specimens while he was an undergraduate student at Brigham used in this study will be deposited at the Brigham Young University. A Nemoura species new to science Young University Collection (BYUC) in Provo, Utah is described in the present paper. Our series of and the Peter Zwick Stonefly Collection (PZSC) in Amphinemura specimens represent new populations Schlitz, Germany. The holotype of Nemoura of Amphinemura baei Ham and Lee 1999 originally jejudoensis sp.n. will be deposited at the United States described from very few specimens. We present a National Museum of Natural History, Smithsonian redescription of this species, provide new Institution, Washington, D.C. USA. illustrations and list the additional collecting Lists of material give the locality names as on the Illiesia – http://www2.pms-lj.si/illiesia/ Volume 7 – Number 15 – Page 148 Zwick, P. & R.W. Baumann. 2011. Nemoura jejudoensis a new species of stonefly and the redescription of Amphinemura baei Ham and Lee (Plecoptera, Nemouridae) from Jeju Island, Korea. Illiesia, 7(15):148-155. Available online: http://www2.pms-lj.si/illiesia/papers/Illiesia07-15.pdf collection labels. Many are in the classical now the medial ends of the anterolateral sclerites. A obsolete transliteration of Korean names. Where central sclerite with median division line appears by necessary, and as far as we were successful, names in transparency. Basal half of rostrum with several the presently valid transliteration are added in oblique folds, in side view seen to be triangularly square brackets. raised near midlength (Fig. 2). Frequently used abbreviations: S, sternite; T, tergite. Sternite 9 almost circular, with short pointed caudal tip. The club-shaped vesicle occupies 4/5 of RESULTS AND DISCUSSION the sternite length. The short blade-like median sclerites of the paraprocts lie alongside the tip of S9. Nemoura jejudoensis sp. n. A membranous section separates them from the (Figs. 1-6) triangular, apically quite narrow outer lobe. Cerci curved forward and mediad, partly Material examined. Holotype ♂, 3♂ paratypes, concealing the epiproct in dorsal view (Fig. 3). Republic of Korea, Cheju Island, Chungmoon City, Medial flat face pale and convex outer face brown, Chonyeon waterfalls, 1 December 1986, M.F. Whiting and pilose. The tiny rudiment of segment 2 is at the [Jeju Island, Seogwipo City, Jungmun, Cheonjeyeon top of the curved cercus, on the soft medial face. falls] Additional material with the same collection There is an inconspicuous subterminal point on the data: 13♀ plus 1♂ and 2♀ used for SEM study. front side of the cercus. Size and habitus. Front wing length 6.4-7.4 (mean Female. Similar to the female of N. geei as illustrated 6.8) mm in ♂, 8.4-9.9 (mean 9.2) mm in ♀. Habitus by Shimizu (1997, his fig. 48). The almost circular typical of genus, not characteristic. Body and pregenital plate on S7 covers the middle of S8 almost antennae brown, legs and palpi paler, yellowish completely, the curved edges of the unpigmented brown. Wing veins brown, membrane of front wing vaginal lobes may be faintly visible (Fig. 5). Sides of with faint brownish tinge. S9 with a poorly defined elongate pigmented strip. Male. Tergite 9 medially short, otherwise Triangular sclerites in the dorsal wall of the genital unmodified. T10 forms the medially largely opening and the end of the oviduct, respectively, membranous base plate of the epiproct. Epiproct may be visible by transparency. closely appressed to base plate, basally firmly In cleared genitalia (Fig. 6), two anterolateral cap- connected, difficult to detach for study. The epiproct like brown sclerites are seen at the side of a long consists of a short body and a long anterior rostrum- carrot-shaped transparent tube connecting to the like process. receptacula. Behind it the dorsal wall of the vagina Body of epiproct short, wider than long (Fig. 1). has two paramedian areas with concentric cuticular The transverse anterodorsal sclerite supports two rings, probably extensible pouches. backwardly turned claw-like plates each with four Diagnosis. A member of the East Asian Nemoura marginal bare teeth. There is an additional ovocercia-group (sensu Shimizu 1997). N. geei Wu anteromedian tooth whose mediodorsal face is beset 1929 occurs on the Asian mainland (China, Korea, with cuticular spicules (Fig. 4). A short distance Russian Far East) as well as on Hokkaido and behind the anterodorsal sclerite lies another slender Honshu. Nemoura pekinensis Claassen 1929 and N. transverse sclerite which is laterally angled brevicercia Zhiltzova 1982 are junior synonyms backward. Other sclerites are seen only in slide- (Shimizu 1997). We studied some specimens from mounted epriprocts, by transparency (Fig. 1). There mainland Korea. Excellent illustrations of N. geei are are no ventral setae or teeth. The basolateral ear- also available (Zhiltzova 1982, 2003, Shimizu 1997, shaped structures that provide the normally-shaped Teslenko & Zhiltzova 2007). Several additional epiprocts of other Nemoura-species with some species occur in Japan, the surrounding islands, mobility can be recognized but seem rigid, non- Taiwan (Shimizu 1997), and China (Li & Yang 2007). functional. Nemoura geei, the present species, N. quadrituberata Rostrum rising from a bulb-like base connected to Shimizu 1997 (Tsushima Island), N. longilobata Illiesia – http://www2.pms-lj.si/illiesia/ Volume 7 – Number 15 – Page 149 Zwick, P. & R.W. Baumann. 2011. Nemoura jejudoensis a new species of stonefly and the redescription of Amphinemura baei Ham and Lee (Plecoptera, Nemouridae) from Jeju Island, Korea. Illiesia, 7(15):148-155. Available online: http://www2.pms-lj.si/illiesia/papers/Illiesia07-15.pdf Figs.1-4. Nemoura jejudoensis sp. n., male. 1. Epiproct in dorsal (right half of figure) and ventral view (left half of figure), respectively. 2. Slightly oblique lateral view of male epiproct, diagrammatic, (not to scale). 3. Dorsal view of abdominal tip. 4. Anterior view of the apical epiproct sclerites. Note that figures 3 and 4 show a strongly contracted specimen with the epiproct pulled down and the paraprocts forward. The normally barely noticed blade-like inner paraproct lobes appear as sharp spines between the cerci and the epiproct while the obtuse pilose apex of the outer lobe is concealed between the epiproct and base of the cercus. Shimizu 1997 (Hokkaido), and N. atristrigata Li & hairy swellings on tergites 9 and 10, toothless cerci, Yang 2007 (Henan) share a very long rostrum of the and broad paraprocts; N. longilobata and N. epiproct in combination with large, dentate, claw- or atristrigata by a large hook on the cercus and broad, hand-shaped anterolateral sclerites. Nemoura tongue-shaped paraprocts. jejudoensis possesses an anteromedial rough process Nemoura jejudoensis differs from N. geei only in the above these toothed sclerites which is absent in N. epiproct. We imagine that N. jejudoensis developed geei. The shape of the transverse dorsal epiproct fairly recently from a small founder population of N. sclerites also differs between the two species. geei that ventured to the off-shore island of Jeju-do. Details of epiproct structure distinguish also the Etymology. The specific name is a noun in other two similar species but they differ in additional apposition after Jeju Island where the specimens characters from N. geei: N. quadrituberata by four were collected that represent this new species. Illiesia – http://www2.pms-lj.si/illiesia/ Volume 7 – Number 15 – Page 150 Zwick, P. & R.W. Baumann. 2011. Nemoura jejudoensis a new species of stonefly and the redescription of Amphinemura baei Ham and Lee (Plecoptera, Nemouridae) from Jeju Island, Korea. Illiesia, 7(15):148-155. Available online: http://www2.pms-lj.si/illiesia/papers/Illiesia07-15.pdf Figs. 5-6. Nemoura jejudoensis, female. 5. Ventral view of abdominal tip. 6. Dorsal view of cleared vagina. Amphinemura baei Ham and Lee brownish tint. Male. T9 medially constricted, caudal margin on (Figs. 7-18) either side of constriction curved, with a few rough Amphinemura baei Ham and Lee 1999:120. Holotype setae (Fig. 11). A rhomboid membranous pale area male; Donneko, Sugyipo-si [Seogwipo City], Jeju-do, remains between T9 and the also constricted T10 Korea. which is medially only a narrow strip in front of the epiproct base plate (Fig. 12). Base plate under the Material studied. Republic of Korea, Cheju Island, epiproct largely membranous. Sogwipo City [Seogwipo City], spring above KAL Epiproct in dorsal view parallel-sided, anterior Hotel fish hatchery, 10 November 1986, M.F. third enlarged in gentle curve which turns abruptly Whiting, 18 ♂, 9 ♀, plus 4♂, 4 ♀ for SEM study; mediad (Fig. 7). Basolaterally, the bases of the ventral Sogwipo City, Tonneko spring [Seogwipo City, sclerites project a little beyond the general contour. Donneko], 18-XII 1986, M.F. Whiting, 1♂; Dorsum densely covered with fine spicules, surface Chungmoon City, Chonyeon waterfalls [Seogwipo divided by fine suture which anteriorly forms a deep City, Jungmun, Cheonjeyeon falls], 28 September cleft separating two short membranous lobes. 1986, M.F. Whiting, 2♂, 1♀; 5 Dec. 1986, M.F. Between these projects, a dorsally split, anteriorly Whiting, 2♂, 2♀. bifid tube. Laterally from it the spinose dark apices of Size and habitus. Front wing length 5.8-6.8 (mean the lateral sclerites project (Fig. 13). From beneath, 6.4) mm in ♂, 6.6-8.2 (mean 7.8) mm in ♀. Habitus as the apices appear as black spinose humps (Figs. 14, typical of genus. Pronotum with very dense coarse 16). In ventral view (Fig. 8), the sclerites converge punctation, rough, matt. Body brown, legs and and narrow anteriorly until they lie close together, antennae brown with indistinctly paler bases, palpi leaving only a narrow space between them. In it yellowish. Wing veins brown, membrane with occur some spines of which mainly the insertion Illiesia – http://www2.pms-lj.si/illiesia/ Volume 7 – Number 15 – Page 151 Zwick, P. & R.W. Baumann. 2011. Nemoura jejudoensis a new species of stonefly and the redescription of Amphinemura baei Ham and Lee (Plecoptera, Nemouridae) from Jeju Island, Korea. Illiesia, 7(15):148-155. Available online: http://www2.pms-lj.si/illiesia/papers/Illiesia07-15.pdf Figs. 7-10. Amphinemura baei. 7. Male epiproct in dorsal view. 8. Same, ventral view; 9. Female genitalia, ventral view. 10. Vaginal complex, cleared specimen in dorsal view; ap, acute process; f, funnel; pg, paragenital plate; rp, rough pocket; sg, subgenital plate; tc, transverse crest. Illiesia – http://www2.pms-lj.si/illiesia/ Volume 7 – Number 15 – Page 152 Zwick, P. & R.W. Baumann. 2011. Nemoura jejudoensis a new species of stonefly and the redescription of Amphinemura baei Ham and Lee (Plecoptera, Nemouridae) from Jeju Island, Korea. Illiesia, 7(15):148-155. Available online: http://www2.pms-lj.si/illiesia/papers/Illiesia07-15.pdf Figs. 11-18. Amphinemura baei. 11. Male terminalia, dorsal, anterior. 12. Male terminalia, dorsal, posterior. 13. Epiproct, lateral. 14. Epiproct, apex, lateral. 15. Right paraproct, dorsal. 16. Epiproct, apex, terminal. 17. Subgenital plate, apex and right paraproct, ventral (cercus removed). 18. Subgenital plate, base, showing vesicle. Illiesia – http://www2.pms-lj.si/illiesia/ Volume 7 – Number 15 – Page 153 Zwick, P. & R.W. Baumann. 2011. Nemoura jejudoensis a new species of stonefly and the redescription of Amphinemura baei Ham and Lee (Plecoptera, Nemouridae) from Jeju Island, Korea. Illiesia, 7(15):148-155. Available online: http://www2.pms-lj.si/illiesia/papers/Illiesia07-15.pdf rings are seen from beneath. The spines themselves strong curvature of the row of spines on the are best seen in lateral view. In side view the epiproct paraproct tip, and by its epiproct. Presence of a is strongly curved, dorsal surface without strong apical sclerite on the lateral epiproct sclerites projections. suggests close relations with A. steinmanni Zwick, A. S9 with a long and slender vesicle, slightly denstigris Zwick, and A. rai Ham and Lee. However, enlarged above base, center of caudal half in these species the apical sclerite is long and tooth- membranous (Fig. 18). Caudal margin of S9 forming shaped. a triangular subgenital plate, that carries the apical Females of the same species are similar, not all gonopore. can be reliably identified by external characters. The Paraprocts complex. Sclerite of outer lobe straight, distinctive inner vaginal structures are known only then bent forward at right angle, the anterior part in a few. Among these, A. denstigris and A. rai also joined to apical part of middle sclerite but junction possess apical funnels that during copula may concealed behind the simple cercus. The middle perhaps receive the apical epiproct tube. Females of sclerite is a slender sinuous band ending in a forward this species are mainly distinguished by the directed sclerite, with an apical row of long spines exceptional length of the funnel, the acute hollow forming a concave line. It surrounds the pilose apex process, and the transverse crest. of the large membranous lobe situated above the We know of no closely similar species from China short triangular inner sclerite (Figs. 15, 17). or Japan. Female (Fig. 9). S8 with distinct pregenital plate. S9 largely covered by sclerotized anteriorly bare Distribution subgenital plates (sg) separated by a short narrow Nemoura jejudoensis and Amphinemura baei appear median slot. Caudal parts drawn out into triangular to be Jeju Island endemics although this requires lobes appearing a bit swallow-tailed, mediocaudal confirmation by future collecting in Korea. Jeju edge gently sinuous. A caudolateral notch exposes a Island is a rather large island located off the southern rounded pilose paragenital plate (pg). coast of Korea. Since stoneflies are distributed by In cleared slide-mounted female genitalia in freshwater connections or as wind-blown adults, the dorsal view (Fig. 10), the paragenital plates form the deep sea water of Jeju Strait probably represents a dorsal most layer in the form of a transparent barrier that is not easily crossed. triangle on each side. Below it are located the much larger dark triangular extensions of the subgenital LIST OF KOREAN STONEFLIES AT THE MONTE plates which carry an acute, apparently hollow L. BEAN LIFE SCIENCE MUSEUM, BRIGHAM process (ap) along the medial edge, near midlength. YOUNG UNIVERSITY, PROVO, UTAH USA There is a transverse crest (tc) on each sclerite. Anteriorly each sclerite supports an oval sclerotized Nemouridae internally rough pocket (rp). Between the latter is a Amphinemura coreana Zwick 1973 funnel (f) formed by a sclerotized cone with a well Republic of Korea, Shichon City, Nam-do, Chong defined straight median tube leading into the base of [not located], 6 April 1997, 3♂, 1♀. the membranous receptacles. Nemoura tau Zwick 1973 Diagnosis. A member of the Amphinemura luteipes- Republic of Korea, Pusan City, Heundae [Busan City, group (Aubert 1967) which is, among other things, Haeundae], at spring behind Garden Mansion distinguished by the tube projecting from the Apts, 18 May 1987, M.F. Whiting, 2♂,4♀. epiproct apex. Most species have a roughly punctate matt pronotum, females of most have a distinct Leuctridae pregenital plate and neatly separate truncate lobes of Megaleuctra saebat Ham et Bae 2002 the subgenital plate. The group is widespread in Asia Republic of Korea, Gangwan-do [Gangwon and includes several Korean species (Zwick 2010). Province], Pyeongchang-gun, Odaesan National The present male is readily distinguished by the Park, trail to Songwon [Songwonsa ] Temple, 13 Illiesia – http://www2.pms-lj.si/illiesia/ Volume 7 – Number 15 – Page 154 Zwick, P. & R.W. Baumann. 2011. Nemoura jejudoensis a new species of stonefly and the redescription of Amphinemura baei Ham and Lee (Plecoptera, Nemouridae) from Jeju Island, Korea. Illiesia, 7(15):148-155. Available online: http://www2.pms-lj.si/illiesia/papers/Illiesia07-15.pdf May 2006, M. Kippenhan, 1♀. that A. baei was the same as our specimens from Jeju Perlomyia mahunkai (Zwick 1973) Island and provided additional constructive Republic of Korea, Pusan City, Heundae [Busan City, criticism. He and Sun Jae Park, National Institute of Haeundae], at spring behind Garden Mansion Biological Resources, Incheon, Republic of Korea Apts, 18 May 1987, M.F. Whiting, 3♂, 25♀. helped us to correctly transliterate Korean names. Republic of Korea, Chollanam Province, small village The scanning electron micrographs and plate North of Sun-Cheon City [Jeonnam Province, containing figures 11-18 were prepared with the help Suncheon City], 12 May 1986, M.F. Whiting, 3♂, of Michael Standing at the Brigham Young 12♀. University Electron Microscope Laboratory. Perlodidae REFERENCES Filchneria stigmata (Ra, Kim, Kang and Ham 1994) Aubert, J. 1967. Les Nemouridae de l'Assam Republic of Korea, Chollanam Province, Sun-Cheon (Plécoptères). Mitteilungen der Schweizerischen City [Jeonnam Province, Suncheon City], 10-15 Entomologischen Gesellschaft, 39:209-253. April 1986, M. F. Whiting, 1♀, eggs on slide. Ham, S.A. and J.B. Lee. 1999. Four new species of Republic of Korea, Seoul, 5 VII 1953, E. L. Shepard, Nemouridae (Plecoptera: Insecta) from Korea. 1♀, without mature eggs. Korean Journal of Biological Sciences, 3:119-125. Li, W.H. and D. Yang. 2007. Two new species of Perlidae Nemoura (Plecoptera: Nemouridae) from Henan, Perlinae gen. sp. (Tyloperla sp. ?) China. Zootaxa, 1511 (21):65-68. Peoples Republic of Korea, 1 mile south Chirummal Shimizu, T. 1997. The species of the Nemoura ovocercia [not located], 1000 ft higher, XI-53, E. Bullock, 8 group (Plecoptera: Nemouridae). Aquatic Insects, larvae. 19:193-218. Kiotina decorata (Zwick 1973) Teslenko, V.A. & L.A. Zhiltzova. 2009. Keys to the Republic of Korea, Pusan City, Heundae [Busan City, stoneflies (Insecta, Plecoptera) of Russia and Haeundae], at spring behind Garden Mansion adjacent countries. Imagines and larvae. Apts, 18 May 1987, M.F. Whiting, 29 June 1987, Dalnauka, Vladivostok, 382 pp. (In Russian.) M.F. Whiting, 1♂. http://www,biosoil.ru/files/00008625.pdf Republic of Korea, Chollanam Province, small village Zhiltzova, L.A. 1982. New species of Stoneflies of North of Sun-Cheon City [Jeonnam Province, Family Nemouridae (Plecoptera) of the Far East. Suncheon City], 12 May 1986, M.F. Whiting, 2♂, Vestnik Zoologi, 1982:37-43. (In Russian, English 1♀. summary). Zhiltzova, L.A. 2003. Plecoptera Gruppe Euholognatha. ACKNOWLEDGEMENTS - Fauna of Russia and neighbouring countries, Special thanks are given to Michael L. Whiting, Insecta Plecoptera, 1 (1):537 + 7 unnumbered Curator of Insects, Monte L. Bean Life Science pages; Russian Academy of Sciences, Zoological Museum, Brigham Young University, Provo, Utah Institute, St. Petersburg, Nauka. (In Russian.) for providing the specimens from Jeju Island upon Zwick, P. 2010. New species and new records of which this paper is based. Boris Kondratieff, Plecoptera from Korea and the Russian Far East. Colorado State University, Fort Collins, kindly Illiesia, 6:75-97. donated the specimen collected by Michael Kippenhan, and Mike provided help with his Korean collecting locality. Shawn Clark, Collections Manager at the Monte L. Bean Museum, made the additional Korean specimens available for study. We are very grateful to David Muranyi, Hungarian Natural Received 6 April 2011, Accepted 15 June 2011, Published 23 June History Museum, Budapest, who helped us realize 2011 Illiesia – http://www2.pms-lj.si/illiesia/ Volume 7 – Number 15 – Page 155