MYOCARDLAL AND SKELETAL MUSCLE BIOENERGETICS ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY Editorial Board: NATHAN BACK, State University of New York at Buffalo NICHOLAS R. DI LUZIO, Tulane University School of Medicine EPHRAIM KA TCHALSKI-KA TZIR, The Weizmann Institute of Science DAVID KRITCHEVSKY, Wistar Institute ABEL LA1THA, Rockland Research Institute RODOLFO PAOLETTI, University of Milan Recent Volumes in this Series Volume 189 COMPARISON OF TYPE I AND TYPE II DIABETES: Similarities and Dissimilarities in Etiology, Pathogenesis, and Complications Edited by Mladen Vranic, Charles H. Hollenberg, and George Steiner Volume 190 WERNER'S SYNDROME AND HUMAN AGING Edited by Darrell Salk, Yoshisada Fujiwara, and George M. Martin Volume 191 OXYGEN TRANSPORT TO TISSUE VII Edited by F. Kreuzer, S. M. Cain, Z. Turek, and T. K. Goldstick Volume 192 MECHANISMS OF STIMULUS-RESPONSE COUPLING IN PLATELETS Edited by 1. Westwick, M. F. Scully, D. E. MacIntyre, and V. V. Kakkar Volume 193 METHODS IN PORPHYRIN PHOTOSENSITIZATION Edited by David Kessel Volume 194 MYOCARDIAL AND SKELETAL MUSCLE BIOENERGETICS Edited by Nachman Brautbar Volume 195A PURINE AND PYRIMIDINE METABOLISM IN MAN V, Part A: Clinical Aspects Including Molecular Genetics Edited by W. L. Nyhan, L. F. Thompson, and R. W. E. Watts Volume 195B PURINE AND PYRIMIDINE METABOLISM IN MAN V, Part B: Basic Science Aspects Edited by W. L. Nyhan, L. F. Thompson, and R. W. E. Watts Volume 196 STEROID HORMONE RESISTANCE: Mechanisms and Clinical Aspects Edited by George P. Chrousos, D. Lynn Loriaux, and Mortimer B. Lipsett A Continuation Order Plan is available for this series. A continuation order will bring delivery of each new volume immediately upon publication. Volumes are billed only upon actual shipment. For further information please contact the publisher. MYOCARDIAL AND SKELETAL MUSCLE BIOENERGETICS Edited by Nachman Brautbar University of Southern California School of Medicine and Hollywood Presbyterian Medical Center Los Angeles, California PLENUM PRESS • NEW YORK AND LONDON Library of Congress Cataloging in Publication Data International Congress on Myocardial and Cellular Bioenergetics and Compartmenta tion (2nd: 1984: University of Southern California) Myocardial and skeletal muscle bioenergetics. (Advances in experimental medicine and biology; v. 194) "Proceedings of the Second International Congress on Myocardial and Cellular Bioenergetics and Compartmentation, held February 16-18, 1984 at the University of Southern California, Los Angeles, California" - T.p. verso. Includes bibliographies and index. 1. Muscles-Congresses. 2. Heart-Muscle-Congresses. 3. Bioenergetics. 4. Cell compartmentation. 5. Tissue respiration. I. Brautbar, Nachman. II. Title: III. Series. [DNLM: 1. Cell Compartmentation-congresses. 2. Energy Metabolism-congresses. 3. Energy Transfer-drug effects-congresses. 4. Myocardium-metabolism-con gresses. WI AD559 v.194/WG 280 1594 1984m] QP321.I525 1984 599'.01852 86-4883 ISBN-13: 978-1-4684-5109-2 e-ISBN-13: 978-1-4684-5107-8 DOl: 10.1007/978-1-4684-5107-8 Proceedings of the Second International Congress on Myocardial and Cellular Bioenergetics and Compartmentation, held February 16-18, 1984, at the University of Southern California, Los Angeles, California © 1986 Plenum Press, New York Softcover reprint of the hardcover 1s t edition 1986 A Division of Plenum Publishing Corporation 233 Spring Street, New York, N.Y. 10013 All rights reserved No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the Publisher To my parents, wife, and children who supported me and stood behind me at all times PREFACE I am pI eased to present to the readers the Proceedings of the Second International Congress on Myocardial and Cellular Bioenerge tics and Compartmentation which was held in Los Angeles during February 16-18, 1984. I would I ike to express my deep appreciation to all those who have encouraged us to hold the Second International Congress. This endeavor could not have been possible without the generous finan cial support of the following: The American Society for Magnesium Research, The University of Southern California Department of Con tinuing Medical Education, Alpha Therapeutics, ALSEB Scientific Products, The American Physiological Society, Ciba-Geigy Corpora tion, Victor Gura, M.D., Merck Sharp and Dohme, Phosphoenergetics, Stuart Pharmaceuticals, and the Upjohn Company. A note of special thanks goes to Samuel Bessman, M.D., who has been a source of constant support and encouragement for this Con gress and for my research endeavors. Nachman Brautbar vii CONTENTS I. HICROCOMPARTHENTATION AND ENERGY TRANSPORT: The Physiological Significance of the Creatine Phosphate Shuttle . . . . . . . . .. . ........ . S. P. Bessman Organization of the Mitochondrial Matrix 13 P. A. Srere and B. Sumegi The Time Course of ATP Cleavage by Contracting Amphibian and Mammalian Skeletal Muscles 27 E. Homsher Isozymes of Creatine Kinase in Mammalian Myocardial Cell Culture • . . . • • • . . . . . • . . . 41 M. W. Seraydarian and T. Yamada Microcompartmentation at the Mitochondrial Surface: Its Function in Metabolic Regulation •• 55 D. Brdiczka, G. Knoll, I. Riesinger, U. Weiler, G. Klug, R. Benz, and J. Krause The Effect of Inorganic Phosphate on Mitochondrial Creatine Kinase . • . • . . . . . . . . . . . 71 N. Hall and M. DeLuca Hormonal Regulation of Creatine Kinase BB 83 A. M. Kaye, N.A. Reiss, Y. Weisman, I. Binderman, and D. Somjen Compartmentation of Adenine Nucleotides and Phosphocreatine Shuttle in Cardiac Cells: Some New Evidence • • • . • • •• •.••.• 103 v. A. Saks, A. V. Kuznetsov, Z. A. Huchua, and V. V. Kupriyanov ix Compartmentation of Hormone Action in Adul t Mammalian Cardiomyocytes • • • 117 I. L. O. Buxton and L. L. Brunton Function of Creatine Kinase Localization in Muscle Contraction • • • • • • • • • • 129 S. Koons and R. Cooke Isotope Labeling Ratios: A Tool for the Exploration of Me ta bol ic Compartments • • • • • • • • • • • • • • • 139 J. K. Kelleher and R. T. Mallet The Mitochondrial Creatine Phosphokinase is Associated with Inner Membrane Cardiolipin •••••• 151 M. Muller, D. Cheneval and E. Carafoli A Role for Mitochondria in Myocardial Adenosine Production • • • • • • • • • • • • • • • • • 157 R. D. Bukoski, H. V. Sparks and L. M. Mela-Riker II. RESPIRATION CONTROL - CELLULAR AND ORGAN LEVEL: Aspects of Heart Respiratory Control by the Mito- chondrial Isozyme of Creatine Kinase •••• 169 W. E. Jacobus, K. M. Vandegaer and R. W. Moreadith Control of Respiration in Intact Muscle • • • • • • 193 M. Mahler Energy Compartmentation and Active Transport in Proximal Kidney Tubules. • • • • • • • • ••••• 213 L. J. Mandel, S. P. Soltoff and P. C. Brazy The Oxygen Dependence of Cellular Energy Metabolism • • • • • 229 D. F. Wilson and M. Erecinska Regulation of Mitochondrial Respiration in Liver • • • 241 A. J. Verhoeven, C. W. T. van Roermund, P. J. A. M. Plomp, R. J. A. Wanders, A. K. Groen and J. M. Tager Quantitation of Fluxes in the Gluconeogenic, Glycolytic, and Pentose Phosphate Pathways in Isolated Rat Hepatocytes: Energetic Considerations ••••••• • • • • • • • • • 255 J. J. Blum and M. S. Rabkin x Why is There a Delay in the Increased Oxygen Consumption During the Rest-Work Transition in Skeletal Muscle? • . • . • • • • • • • • • • • • • • • • • . • . • 271 R. J. Connett III. MYOCARDIAL PRESERVATION, ISCHmrrA: CELLULAR MECHANISMS Compartmentation and Functional Mechanisms in Myocardia I F ai I ure and Myocardial Infarction .••••• 283 R. J. Bing, Y. Sasaki, M. Chemnitius and W. Burger Myocardial Protection of Hypertrophied Hearts by Administration of Cardioplegia According to Regional Myocardial Temperature ••••••••• 297 G. Gorlach, H. H. ScheId, F. Polzuweit, J. Mulch, W. Schaper, and F. W. Hehrlein Heart Myocytes as Models of the Cellular Response to Ischemia • • • • • • • • • •• ••••• 303 G. P. Brierley, W. C. Wenger and R. A. Altschuld Biologic Basis for Limitation of Infarct Size • • • • • • • • • 315 K. A. Reimer and R. B. Jennings Mitochondrial Transmembrane Proton Electrochemical Potential, Di- and Tricarboxylate Distribution and the Poise of the Malate-Aspartate Cycle in the Intact Myocardium • • • • • • • • • • • •• • ••• 331 R. A. Kauppinen, J. K. Hiltunen and I. E. Hassinen Sarcomere Length-tension Relationship in Toad Atrioventricular Pacemaker: Length Dependent Activation • • • • • • 343 J. R. Lopez and D. Lea Myocardial Acidosis and the Mitigation of Tissue ATP Dep I etion in Ischemic Cardiac Musc Ie: The Role of the Mitochondrial ATPase •••••••••••• 355 W. Rouslin Vascular Metabolism and Energetics • • • • • • • • • • • • • • 375 R. J. Paul, R. M. Lynch and J. M. Krisanda Myocardial Glutamate Dehydrogenase Activity ••••••••• 389 H. G. McDaniel and R. L. Jenkins xi Long Term Model for Evaluation of Myocardial Metabolic Recovery Following Global Ischemia •••••• 401 J. St. Cyr, H. Ward, J. Kriett, D. Alyono, S. Einzig, R. Bianco, R. Anderson, and J. Foker The Effect of Reperfusion and Streptokinase on Ischemic Myocardium Serum Creatine Kinase Activity, MM Subtypes and Myocardial Blood Flow ••••••••••• 415 J. Mickelson, C. J. Carlson, B. Emilson and E. Rapaport Post-contracture Reperfusion: Effect of Elevated Potassium and Verapamil. • • • • • • •••• 427 S. B. Digerness, W. G. Tracy, N. F. Andrews and J. W. Kirklin Ultrastructure of the Human Myocardium after Intermittent Ischemia Compared to Cardioplegia. • ••.•••.• 439 G. Gorlach, H. H. Scheld, J. Mulch, J. Schaper, and F. W. Hehrlein IV. PATHOPHYSIOLOGY OF ENERGY COMPAR'l'MENTATION: Cell ular Injury in Phosphate Depletion: Patho- genesis and Mechanisms in the Myocardium ••••• 451 N. Brautbar Diabetes Mell i tus and Hypothyroidism Induce Changes in Myosin Isoenzyme Distribution in the Rat Heart - Do Alterations in Fuel Flux Mediate These Changes? ••••••••••••••••••••• 469 W. H. Dillmann Glycerol Kinase Deficiency: Compartmental Considerations Regarding Pathogenesis and Clinical Heterogeneity •••• 481 E. R. B. McCabe, and W. K. Seltzer Phosphorylated Nucleotides and Glycolytic Intermediates in Diabetic and Non-diabetic Rat uterus in Late Pregnancy . . . . . . . . . . . . . . . . . . . . . . . . 495 I. Zaidise, P. J. Geiger, D. Boehme and S. P. Bessman Effect of Calcium Antagonists on Vasopressin Induced Changes in Myocardial and Renal Pyridine Nucleo- tides in the Intact Rat •••••••••••••••.• 503 D. DiPette, R. Townsend, J. Guntipalli, K. Simpson, A. Rogers and E. Bourke xii