United Arab Emirates University Scholarworks@UAEU Theses Electronic Theses and Dissertations 2-2014 Molecular Characterization of VIM Carbapenemases in the Arabian Peninsula Nour Yahfoufi Follow this and additional works at:https://scholarworks.uaeu.ac.ae/all_theses Part of theMedicine and Health Sciences Commons Recommended Citation Yahfoufi, Nour, "Molecular Characterization of VIM Carbapenemases in the Arabian Peninsula" (2014).Theses. 262. https://scholarworks.uaeu.ac.ae/all_theses/262 This Thesis is brought to you for free and open access by the Electronic Theses and Dissertations at Scholarworks@UAEU. It has been accepted for inclusion in Theses by an authorized administrator of Scholarworks@UAEU. For more information, please [email protected]. UniteAdr abE miratUensi versity Colleogf M edicianned H ealtShc iences DepartmeonfMt i.c orbioloagnyd I mmunology MOLEUCL R CHARACTERlAZn ON OFV IMC ARBAPNEEMASEISN THEA RABAINP E INSULA NoruY ahofuif ThiTsh eissi ss ubimtteidnp atrifaullfi lenmotft hree quirefmorte hnet stM eaorsf Medciinaen Hde alctihe nicnMe isco rbiloogayn Id mmnuoglyod egree Undetrh ee dcitiroofPn r eosfsToirob rP al Febarru2y0 14 -i- DeclaratoifoO nr iginWaolr k Page ouYra hofuift,h uen deirg neag dr,a dusattueda ettn htUe n eidtA rab Eamtiers J, Unie ri t(yU AE) a ndt haeu thooftr h teh edsiis se/rtattiidto "lnMe ocluerl a haarcteriozfaV t]iMoC na rbeanpemeas inA ratbhieaP nen nisulhaee"rb y olemndlelycae r thatth itsh ie/ id erttiaoinsa no rigiwnoarlkd onaen d prepabr meedu ndtehrge u idaonfc eP Troibf.oP ra iln Ctohllege eo fM edciien andH ealht ceinceDse,p tamrenoftM icorbigoyla ondI mmunoloAgtUy A EU. ThiW orkh a notp reivousflonyn etdh bea sfiorst he awoafar ndyd egree, diopmlao ri mliatrie t altt hiosra nyo thuenrei rviyst.T heM atreiablosr rowed form otheoruc resan di cnlduedi nm y thedsiissset/ratihoanv e bpeorepne rly .;o� ... a knolwdeged. 09. tudenitg'tnsuae r. ..... ). . Date ./)�..lcJ. of L. I -i-i Copyr©i g2h01t4b y ouYra hofuif AlRli hgtRse served -i-ii SignatPuargee s ApproveBdy dvio ryC ommittee: 1)A dvoir ' TiborP alM D, PhD Title' Professor DepartmeMnitrc:o bioalnoIdgm ymn uoolgy Int itutioCno:l gleoe fM edicainnHdee a lh tcienUcAeEsU, Dat2e8-0 12-014 2)M emebr: Bassma AliP hD Title: Professor DepartmePnatt:h ology Int itutioCno:lg leoe fM edicainnHdee a lhS tcienUcAEeUs , #/� : ignature te2 8-012-014 3) Memb:e r AgnesS onnevenMDd, PhD Tilt:e AssisPtraonfte ssor DepartmeMnitc:r obiaonlIdom gmyuo ngoyl IsntitutiCoonll:ge eo fM edicainnHdee ahlS tcienUcAEeUs , Signature -iv- Thei Examinatioonm mittee: 1)A dyoir . TiboPra lM D PhD TIlte: Profoer DepartmeMnItc:r obiaonIldom ugmnyoo gly InstitutCloOllnge:eo f MedaincHdie anlheS t cienUcAeEsU, Dat2e80 -12-014 2)M ember: BassamA liP hD Title: Profoers DepartmePnatt:h ology InstitutCioollnge:eo fM edicainnHdee ahlS tcienUcAeEsU,. Signature Dat2e8- 012-014 A 3)M ember: gnesS onnevenMdD ,P hD Title: AssisPtraonfte ssor DepartmeMnitc:r olgboyia onIdm muongoyl IsntitutioCnol:lge eo fM edicainnHdee alStchi enUcAeEsU, Signature Dat2e80 -12-014 cont. 4)E xteErnxamlai ner:D avidL esliPea tersMoDn, PhD Title: Professor Department: CentfeoCrril n icRaels earch -v- Int lttiu.o n Unie rsoifQt eye snalndA,us tralia ignature Dat2e8-0 12-014 Acceptebdy : MastePrrv-f'osg rDa imrt eoDcrr M.a yramA lS hamsi /') 2 igna.t.u. re � � .........D .at.e. ./ 2!ZOI!t Deaonft hCeo lglePe:r oT ar-Ching Aw I( &0 q � igna.t.u.. r...e. ... ..... . . .... Date l'f ( Copy l1o f 12 __ __ __ __ -vi- ABT RACT The emegrence agonlbdla spreaodf carbaperneesmi stant Entearcotberiaic aenaa el naningw orld-pwhiedneo metnhoaantlo s affecttsh e MiddlEea t duet ol miitetdr eatomepntti oforn sucihne fctiaonndst heir commoan o ciawtiitohhn gi hl eevlof f atlatiy. mTohset p oitrmanmtec hiasnomf ucrhse itsanicsce a eudsb yt hpeor ductoifvo anr icoaubr apeneemnayzsmee sI.n thAera biPaenni nls,asu of ar,N DMa nOd XA4-8ilekc abrapenemhaasvbeees e n reporwtheiedl e, les\h eer otheenrz ymee.sg,V. I MI, MP andK PC area los commonflou ndO.u ra imw ast os yesmtatliylc laookf or tVheIM -type carbpaenemaasmeosn glc oali slotaesa ndt o chaacrtertihzeeig ren iect bacrkogund. Inailyt,lis crneienigs otlefasor m AbuD habhio sapliswt ei denetdai sfiingle Enterobcalcotaecrse tairen ac arryintgh VeI M-4 aleell.T hiwsa st hfei rssutc h isaotleev erre porftrmeo tdh Peen insSuublsaeu.qe n,ti lvnyestiingigas totlefasor m Kuwa,Si atuAdraib ,iO amana ntdh UeA E,w ei deinefitda f urth1e1irs otleoasn,e E.c loacfreoameS auAdria bitaw,of o rmO mano,n efor mK uwaaintda los one E chericchoilfaoir mt hciosu rnytB.e sitdheess el,ta ttcehoreu r nyat losp rovided sxiK lebsipenleluami oanie sotleAasl.s lt irnapsr oudcetdh VeJ M-v4ar iaonftt h e enzymea sd eteirnmebdyt hsee quenocfti ehniggr en esI.na lcla setsh,e gweanse lcoaetd opanl smiodfsv ayrinsgie zse,ti henro ny-ptabolreb elongtoit nhge lnAciC gorupm;os to f twheeemrc ojnguaiteva ntdh ecyo mmonhlaryb oroetdh er 13-laamcatsgeen ess,u cahsC TX-oMr C-M4YI. na lslta rintshVe IM -4g enwea s locawtitehdia c nal sIsi tnegr-owni tsho mvear iatbieotnwset ehgnee ncea steets prese-nsti lmaitro s tirnapsr eovuisliyd eiinfetdi nN ortAhfr icaan dIa tyl, -vii - sugget ign thei bpiiotol yfs pera.dC lnoatlpy inrgee altehdat hte l airetvelhyi gh icnidenocfe V-IpMruocdeErn treobcateriea ecnecaoeurneitdnK uv.iawta sn ot duet ot hsep erad opfat riaac ru olcnleb,u mto sptr oabblwya st hrese uolftt he trafnero fa nI cn C pImai dc,o -hoarribbnlga vaIMn4db l acM.iY tn-4oK lebsiella pneumoena inaEd. c oli. Ourd atsah otwh ,ab teyoNnDdM a ndO XA-4i8ek-,Vl IMt ypceab rapenemases arteh teh dim rosctom mon oliaestsi nt hAer aibnaP enisnulat.h eiFvrnue risgtation i needteomd o niotrt hsep erad colnfoe asn gde neisn tghieo nr.e -vii-i ACKNOWLED GME TS I takteh iosp ptouryn ittoe xpremsys p rooufngdr iattduet o msyu pveIorsr PreosfsToirob rP al,a sw ellas D r.g neso nnveenfdor t ehigru idaanncde monitnogr.i Fosru er,Iw oulhda e b eeunn ablaec ihteovte h is wtitahsotkuh te siuerpvr ision as waesl lth heelo pft hger oumpe mebrasn ds tfai fnb acrtieoglyl oaboratories, M A. kelaG hzaawMir,. M ohammedA I-Hjaa ndM rs.D aniDaa wricSho.m, y graicoutsh antko eer oyn oef t hem. Thantkosfo or t he cotnartirnuosesld t,oP r.oV finceRnott imDir,W. a afJ amal, Proft.e fh ibl,S eDiArfIA. -b rDir,A. m niaA IJ-aardnDir,S. a fiGniraigz,s D r. Rah aHn ashmyea ndD rW.a heedi qT.a r Iw oulda los liet koe xtemnyds icneer greientgtsoP ro.B fassamA li aanldl t o fauctlym emebrisnt hDee ptamreonftM icorbiloogayn Id mmunloogCyo,ll geeo f Mediicena nHde alStcehinc esU,ni tAedra b Eamtiers eUsrnyiit.v Ina ddiiotnd eae tph aynoktu o my baronatdhl melyr sif ernds. NouYra hofuif -ix-