Molecular aspects of interspecific hybridization of sturgeons related to polyploidy and in situ conservation Molekulární aspekty mezidruhové hybridizace jeseterovitých ryb ve vztahu k polyploidii a in situ konzervaci Stu r geon gene i cs and cy t ogene ics – T e Neverending Stor y Miloš Havelka Vodňany, Czech Republic, 2013 - 1 - - 1 - -- 22 -- - 3 - Molecular aspects of interspecific hybridization of sturgeons related to polyploidy and in situ conservation Molekulární aspekty mezidruhové hybridizace jeseterovitých ryb ve vztahu k polyploidii a in situ konzervaci Miloš Havelka VVooddňňaannyy,, CCzzeecchh RReeppuubblliicc,, 22001133 -- 22 -- - 3 - -- 44 -- - 5 - I, Miloš Havelka, thereby declare that I wrote the Ph.D. thesis myself using results of my own work or collaborative work of me and colleagues and with help of other publication resources which are properly cited. I hereby declare that, in accordance with the § 47b Act No. 111/1998 Coll., as amended, I agree with publicizing of my Ph.D thesis in full version electronically in a publicly accessible part of the STAG database operated by the University of South Bohemia in České Budějovice on its web sites, with keeping my copyright to the submitted text of this Ph.D. thesis. I also agree so that the same electronic way, in accordance with above mentioned provision of the Act No. 111/1998 Coll., was used for publicizing reviews of supervisor and reviewers of the thesis as well as record about the progress and result of the thesis defence. I also agree with compering the text of my Ph.D. thesis with a database of theses “Theses.cz” operated by National Register of university theses and system for detecting of plagiarisms. In Vodňany 27th April, 2013 -- 44 -- - 5 - -- 66 -- - 7 - Supervisor: Assoc. Prof. Dipl.–Ing. Martin Flajšhans, Dr.rer.agr. University of South Bohemia in České Budějovice (USB) Faculty of Fisheries and Protection of Waters (FFPW) Research Institute of Fish Culture and Hydrobiology (RIFCH) South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses (CENAKVA) Zátiší 728/II 389 25 Vodňany Czech Republic Consultant: Dipl.-Ing. Martin Hulák†, Ph.D. University of South Bohemia in České Budějovice (USB) Faculty of Fisheries and Protection of Waters (FFPW) Research Institute of Fish Culture and Hydrobiology (RIFCH) South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses (CENAKVA) Zátiší 728/II 389 25 Vodňany Czech Republic Head of Laboratory of Molecular, Cellular and Quantitative Genetics: Assoc. Prof. Dipl.-Ing. Martin Flajšhans, Dr.rer.agr. Dean of Faculty of Fisheries and Protection of Waters: Prof. Dipl.-Ing. Otomar Linhart, DSc. Board of doctorate study defense with referees: Assoc. Prof. M.Sc. Zdeněk Adámek, CSc. – head of the board Assoc. Prof. Dipl.-Ing. Lukáš Kalous, Ph.D. – board member Assoc. Prof. Dipl.-Ing. Pavel Kozák, Ph.D. – board member Assoc. Prof. M.Sc. Jana Pěknicová, CSc. – board member Assoc. Prof. M.Sc. Josef Matěna, CSc. – board member Assoc. Prof. Tomáš Randák, Ph.D. – board member Assoc. Prof. Stanislav Navrátil, DVM, CSc. – board member Assoc. Prof. Dipl.-Ing. Lukáš Kalous, Ph.D. – national referee Prof. Katsutoshi Arai, Ph.D. – international referee Date, hour and place of Ph.D. defense: 11th September 2013 at 14:00 in USB, FFPW, RIFCH, Vodňany Name: Miloš Havelka Title of thesis: Molecular aspects of interspecific hybridization of sturgeons related to polyploidy and in situ conservation Ph.D. thesis, USB, FFPW, RIFCH, Vodnany, 2013, 102 pages, with the summary in English and Czech. -- 66 -- - 7 - CONTENT CHaPTEr 1 9 1.1. Introduction 11 1.2. Evolution of sturgeon genome 14 1.3. Karyology and ploidy levels of Acipenseriformes 15 1.4. Interspecific hybridization of Acipenseriformes 19 1.5. Aims of the thesis 22 References 23 CHaPTEr 2 31 Extensive genome duplication in sturgeons: new evidence from microsatellite data CHaPTEr 3 39 First evidence of autotriploidization in sterlet (Acipenser ruthenus) CHaPTEr 4 49 Fertility of a spontaneous triploid male Siberian sturgeon (Acipenser baerii) CHaPTEr 5 71 Investigation of the segregation pattern of microsatellite alleles during experimental hybridization of polyploid sturgeon species CHaPTEr 6 81 General Discussion 83 English Summary 92 Czech Summary 94 Acknowledgements 96 List of Publications 97 Training and Supervision Plan during Study 99 Curriculum Vitae 101 -- 88 -- - 9 - CHaPTEr 1 GENEraL iNTrODuCTiON -- 88 -- - 9 - -- 1100 -- - 11 -