This page intentionally left blank The Latest Evolution in Learning Evolve provides online access to free learning resources and activities designed specifically for the textbook you are using in your class. The resources will enhance your learning of the material covered in the book and much more. Visit the website listed below to start your learning evolution today! LOGIN: http://evolve.elsevier.com/AU/Rozenberg/haematology/ Evolve Online resources for Microscopic Haematology 3e include: For students and instructors • 13 interactive case studies: Includes progressive case history, analyser data, blood film, blood film comments and MCQs with instant feedback • Student supplement, which includes: o Automated analysis of blood o Impedance o Haemoglobin concentration o Differential white cell count o Nucleated red blood cells o Summarising haematology analyser values o Blood film examination o Preparation of a blood film of an acceptable standard for diagnostic pathology o Inclusion bodies o Polychromasia o Dimorphic blood picture o Manual differential white cell count o Leucoerythroblastic blood picture o Examination of platelet morphology o Reporting For instructors only • 18 case studies with template for diagnosis, classification, treatment and/or therapy options and prognosis • images for instructor case studies • reporting template • image collection from book Think outside the book...evolve This page intentionally left blank Gillian Rozenberg Sydney Edinburgh London New York Philadelphia St Louis Toronto Churchill Livingstone is an imprint of Elsevier Elsevier Australia. ACN 001 002 357 (a division of Reed International Books Australia Pty Ltd) Tower 1, 475 Victoria Avenue, Chatswood, NSW 2067 This edition © 2011 Elsevier Australia This publication is copyright. Except as expressly provided in the Copyright Act 1968  and the Copyright Amendment (Digital Agenda) Act 2000, no part of this publication  may be reproduced, stored in any retrieval system or transmitted by any means (including  electronic, mechanical, microcopying, photocopying, recording or otherwise) without prior  written permission from the publisher. Every attempt has been made to trace and acknowledge copyright, but in some cases this  may not have been possible. The publisher apologises for any accidental infringement  and would welcome any information to redress the situation. This publication has been carefully reviewed and checked to ensure that the content is as  accurate and current as possible at time of publication. We would recommend, however, that  the reader verify any procedures, treatments, drug dosages or legal content described in this  book. Neither the author, the contributors, nor the publisher assume any liability for injury  and/or damage to persons or property arising from any error in or omission from this publication. National Library of Australia Cataloguing-in-Publication Data ___________________________________________________________________  Rozenberg, Gillian. Microscopic haematology: a practical guide for the laboratory / Gillian Rozenberg. 3rd ed. 9780729540728 (pbk.)   Blood--Diseases--Diagnosis   Blood--Examination--Atlases. 616.150758 ___________________________________________________________________ Publisher: Melinda McEvoy Developmental Editor: Rebecca Cornell Publishing Services Manager: Helena Klijn Project Coordinator: Natalie Hamad Edited by Sybil Kesteven Proofread by Sarah Newton-John Cover and internal design by Lisa Petroff Index by Annette Musker Typeset by TNQ Books & Journals Printed by China Translation and Printing Services CONTENTS Preface  vii Abbreviations  ix Reviewers  xiii Part A Erythrocytes   A1  Erythropoiesis  2   A2  Deficiency anaemias  8   A3  Haemolytic anaemias  12   A4  Haemoglobin disorders  23   A5  Red cell membrane disorders  34   A6  Miscellaneous red cell abnormalities  42 Part B Leucocytes and platelets   B1  Myeloid cells  46   B2  Monocytes and macrophages  92   B3  Platelets  101   B4  Lymphocytes  106   B5  Plasma cells  142   B6  Acute myeloid leukaemia (AML) and related precursor neoplasms  150 Part C Haematology relating to paediatrics   C1  Red cell disorders in the neonate and childhood  185   C2  Bone marrow failure  191   C3  Benign disorders of leucocytes in the neonate and childhood  199   C4  Myeloproliferative neoplasms in the neonate and childhood  205   C5  Non-haemopoietic malignancies in the neonate and childhood  210   C6  Storage disorders in the neonate and childhood  218   C7  Platelet abnormalities in the neonate and childhood  223 Part D Blood parasites   D1  Malarial parasites  230   D2  Non-malarial blood parasites  244 Index  249 vii This page intentionally left blank PREFACE The third edition of Microscopic Haematology: A Practical Guide for the Laboratory, main- tains the standard and picture quality achieved in the second edition. The third edition   includes descriptions of neoplasms according to the fourth edition of the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. Additional red cell disorders and white  cell neoplasms, including non-Hodgkin lymphoma have been included in this edition. An  additional ninety-two images have also been included. I am indebted to a number of people for their assistance in compiling this book. I  would like to thank Professor Robert Lindeman (Director of the Department of Haema- tology at the Prince of Wales Hospital, Sydney) for allowing me to access all the blood  films and bone marrow slides in our laboratory. I am especially indebted to Michael Oakey  and Virginia Bentink for their invaluable help and experience in producing a CD-ROM  of all 450 images. Thank you to Pauline Dalzell for her expert assistance in updating the  cytogenetics of haemopoietic and lymphoid neoplasms. Above all, I wish to thank Narelle  Woodland (Senior Lecturer and Coordinator of Haematology at the University of Technol- ogy, Sydney) for her advice and continued support during my writing of this third edition. Gillian Rozenberg ix