DOCUMENT RESUME ED 268 336 CE 044 218 AUTHOR Thompson, Joselyn H. TITLE Medical Laboratory Technician--Microbiology (AFSC 90470). INSTITUTION Air Univ., Gunter AFS, Ala. Extension Course Inst. PUB DATE 28 Nov 83 NOTE 644p.; Supersedes ED 224 892. PUB TYPE Guides Classroom Use - Materials (For Learner) (051) EDRS PRICE MF03/PC26 Plus Postage. DESCRIPTORS *Allied Health Occupations Education; Behavioral Objectives; Biomedical Equipment; Correspondence Study; Distance Education; Laboratory Procedures; *Laboratory Technology; Learning Activities; *Medical Evaluation; Medical Services; *Medical Technologists; *Microbiology; Military Personnel; *Military Training; Postsecondary Education IDENTIFIERS Air Force ABSTRACT This four-volume student text is designed for use by Air Force personnel enrolled in a self-study extension course for medical laboratory technicians. Covered in the individual volumes are laboratory procedures in clinical bacteriology (the history of bacteriology; aseptic techniques and sterilization procedures; bacterial morphology and physiology; innoculation of media fen' isolation of bactaria; antimicrobial agents, drug resistance, and susceptibility testing; gram-positive organisms, gram-negative cocci, coccoid forms, and bacilli; enteric organisms, mycobacterium and spirochetes); clinical parasitology (medical parasitology and protozoa, platyhelminthes, and nematodes infecting man); and laboratory procedures in clinical mycology (medical mycology, yeastlike fungi and monomorphic molds, pathogenic dimorphic fungi, saprophytic fungi, and laboratory diagnosis of viral rickettsial and chlamydial diseases. Each volume in the set contains a series of lessons, exercises at the end of each lesson, a bibliography, and answers to the exercises. Supplementary volume review exercises, a foldout, and a change supplement are also provided. (MN) *********************************************************************** Reproductions supplied by EDRS are the best that can be made from the original document. *********************************************************************** MEDICAL LABORATORY TECHNICIAN-- MICROBIOLOGY (AFSC 90470) EXTENSION COURSE INSTITUTE AIR UNIVERSITY U.S. DEPARTMENT OF EDUCATION N TIONAL INSTITUTE OF EOUCATION Elp CATIONAL RESOURCES INFORMATION CENTER (ERIC) This document has been reproduced as received from the person or organization originating it I ] Minor changes have been made to improve reproduction quality Points of view or Winans stated in this docu mnt do not ncssanly represent off !cal Nli pONOOn or policy 2 [ ....moliFrrih-_ Ea COURSE MATERIALS SHIPPING LIST EFF1-rTivt USTI- OUPCF TITL F ( Cr)nR'.1- NUSARFR MEDICAL LABORATORY TECHNICIAN--MICROBIOLOGY 23 Nov 83 90412 (AFSC 92470) this list immediately upon re, riving Check The following materials are needed to complete this course. INSTRUCTIONS Immediately Use the incorrect (numbers don't match) notify ECI are missing or your course package, and II any materiel& oi,I number, Ea Form 17 for thii, purpose. and be sure to include your identification number, address, course and %olume Send WI correspondence separately from your answer sheet.' VRE form designation (If a VRE la involved). INVENTORY CONTROL VRE ANSWER SHEET DESIGNATION OR TITLE TYPE ITEM NUMBER IDENTIFICATION ECI RETURN ENVELOPES (4 EACH) ENV 1 - ---, M-140 YOUR KEY TO A SUCCESSFUL COURSE 2 _____ __ VOL 1, Laboratory Procedures in VOL 90412 01 7809 Clinical Bacteriology (Part I) 3 ___ 90412 01 22 90412 01 22 VRE VOLUME REVIEW EXERCISE (VOL 1) 4 VOL 2, Laboratory Procedures in VOL 90412 02 7910 Clinical Bacteriology (Part_ II) 5 90412 02 S02 7910 SUPPLEMENTARY MATERIAL (VOL 2) SUPP 7 _8_ 90412 02 90412 02 22 VOLUME REVIEW EXERCISE (VOL 2) VRE. 90412 03 8007 VOL VOL 3, Clinical Parasitology 9 90412 03 22 90412 03 22 10 VRE VOLUME REVIEW EXERCISE (VOL 3) VOL 4, Laboratory Procedures in VOL 11 90412 04 8201 Clinical Mycology SUPPLEMENTARY MATERIAL (VOL 4) 90412 04 SO1 8201_ SUPP 12 (Foldouts 1-3) 90412 04 22 90412 04 22 VOLUNL REVIEW EXERCISE (VOL 4) VRE 13 - - DIRECT ANY QUESTIONS OR COMMENTS NOTE: RELATING TO ACCURACY OR CURRENCY OF 4- TEXTUAL MATERIALS TO AUTOVON 736-2809. SPECIAL NOTE: DISREGARD THE AFSC 90412 PRINTED ON THE COVER OF THE IcLRAIIIMER OIL THIS CDC IS TEXTS. UPGRADE TRAINING TO AFSC 92470 ONLY. 5/d. RI:VERSE SIDE FOR ADDITIONAL INSTRUCTIONS. 4 PAGES FORM PAGE I OF PREVIOUS EDITION IS OBSOLETE. E CI Jul. Of 3 LIST OF CHANGES COURSE CAREER FIELDS. POLICIES. PROCEDURES AND EQUIPMENT CHANGE. ALSO ERRORS NO. OCCASIONALLY GET INTO PRINT. THE FOLLOWING ITEMS UPDATE AND CORRECT YOUR COURSE MATERIALS. PLEASE MAKE THE INDICATED CHANGES. 90412 'FFECT:vE DATE OF SNIPPING UST 28 Nov 83 NOTE: YOU ARE NOT REQUIRED TO POST ANY CHANGES LISTED IN THIS SHIPPING LIST WHICH CORRECT TYPOGRAPHICAL ERRORS, UNLESS SUCH ERRORS CHANGE OR OTHERWTsE AppprT THE MEANING OF THE MATERIAL. THROUGHOUT THE VOLUMES, WHEREVER IT APPEARS, CHANGE THE FIRST 3 SPECIAL NOTE: DIGITS OF ALL AFSC REFERENCES FROM 904 TO 924. VOLUME 2 CHANGE FOR THE TEXT: 1. "Foldouts 1 and 2 are printed Change to read: Preface, page iii, line 9: Foldout 3 appears in the back of the volume." as separate enclosures. VOLUME 3 CHANGE FOR 'OE TEXT: 2. Change "419" to "417." Page 20, col 1, line 11: VOLUME 4 CHANGES FOR THE TEXT: 3. Change "muslce" to "muscle." Page 10, col 2, line 18 from bottom: a. Change "macroscopically" to "microscopically." Page 21, Exercises (612)-6: b. Change "en" to "an." Page 52, col 1, line 5 from bottom: c. change "Macroscopically" to Page 90, col 1, line 3 from bottom: d. "Microscopically." Change "PAPOVARIVUSES" to Page 92, Table 5-1, under "Group" column: e. "PAPOVAVIRUSES" and change "PDX VIRUSES" to "PDXVIRUSES." Change "comoplete" to "complete." Page 98, col 1, line 9: f. Change "Burkett's" to "Burkittts." Page 100, col 1, line 12 from bottom: g. VOLUME 2 CHANGE FOR THE VOLUME REVIEW EXERCISE: 4. After "Bacteriology" add "(Part II)." Cover page, line 6: VOLUME 3 CHANGES FOR THE VOLUME REVIEW EXERCISE: 5. In the stem of the question, change "Whih" to Page 3, question 10: a. In the stem of the question, delete "of infection." Question 14: "Which." Change "Gingitis" to "Gingivitis." Page 6, question 34, choice c: b. Change "chromoloidal" to "chromotoidal." Page 7, question 38, choice c: c. Question 39, choice c: Change "hartamanni" to "hartmanni." of (Page 2 4 ) of ECI Form 25 LIST OF CHANGES COURSE CAREER FIELDS, POLICIES, PROCEDURES AND EQUIPMENT CHANGE. ALSO ERRORS NO. OCCASIONALLY GET INTO PRINT. THE FOLLOWING ITEMS UPDATE AND CORRECT 90412 YOUR COURSE MATERIALS. PLEASE MAKE THE INDICATED CHANGES. 1FFECTIVE DATE OF SHIPPING UST 28 Nov 83 CHANGES FOR THE VOLUME REVIEW EXERCISE: 5. VOLUME 3 (Continued) Page 9, question 55: d. In the stem of the question, after "parasite" add "consists of two series of events which" and change "takes" to "take." Page 10, question 59, choice b: e. Change "flaciparuat" to "falciparum." Page 15, question 92: f. In the stem of the question, change "kateral" to "lateral." Question 96: In the stem of the question, change "American ?" to "America?" Page 16, question 101: g. In the stem of the question, change "thenubmer" to "the number." Page 17, question 108: h. In the stem of the question, add "that" after "the." 6. CHANGES FOR THE VOLUME REVIEW EXERCISE: VOLUME 4 Page 2, question 6, choice c: a. Change "6,5" to "7.5" Choice d: Change "6.8" to "8.5." Page 3, question 11, choice c: b. Change "Nocarida" to "Nocardia." ige 5, question 21: c. In the stem of the question, change "microscopically" to "macroscopically." Page 6, question 28: d. In the stem of the question, change "Candidablicans" to "Candida albicans." Question 32, choice c: Change "xylose" to "maltose." Page 7, question 36, choice d: e. Change "incased" to "encased." Question 37, choice a: Change "arthnospores" to "arthrospores." Page 8, question 42: f. In the stem of the question, change "antibotics" to "antibiotics." Question 43: In the stem of the question, change "M. gyseum" to "M. gypseum." Page 10, question 56: g. In the stem of the question, change "nocordiosis" to "nocardiosis." h. Page 11, question 57, choice d: Change "subjeced" to "subjected." Question 60, choice a: Change "Ceserin" to "Casein." i. Page 13, question 70, choice c: Change "sensitie" to "sensitive." Page 16, question 88: j. In the stem of the question, change "revese" to "reverse." 3 of (Page 4 of ECI 'win ) 2s 5 LIST OF CHANGES COURSE CAREER FIELDS, POLICIES, PROCEDURES AND EQUIPMENT CHANGE. ALSO ERRORS NO. OCCASIONALLY GET INTO PRINT. THE FOLLOWING ITEMS UPDATE AND CORRECT 90412 YOUR COURSE MATERIALS. PLEASE MAKE THE INDICATED CHANGES. FICTIVE DATE OF SHIPPING LIST 28 Nov 83 CHANGES FOR THE VOLUME REVIEW EXERCISE: 6. VOLUME 4 (Continued) Page 17, question 97, choice a: k. Change "growth rate" to "growth at 42°C." Question 99: In the stem of the question, change "mononucleosis" to "mononucleosis." Choice Change "herpesviruses" to "Herpesviruses." : Page 18, question 105, choice b: 1. Change "nuycleocapsid" to "nucleocapsid." Page 19, question 111: m. In the stem of the question, change "nomonucleosis" to "mononucleosis." Page 20, question 117, choice a: n. Change "arthopod" to "arthropod." Choice c: Change "sperical" to "spherical." Question 17 is no longer scored and need not be answered. o. NOTE: Change the currency date on all volumes to "May 1983." 6 4 of (Page 4 ) I ECI Pena 25 90412 01 7809 CDC 90412 - MEDICAL LABORATORY TECHNICIAN MICROBIOLOGY (AFSC 90470) Volume 1 Laboratory Procedure,. in Clinical Bacteriology (Part I) Extension Course Institute Air Training Command 7 Prepared by S MSgt Joselyn H. Thompson School of Health Care Sciences (ATC) Sheppard AFB, Texas 76311 Reviewed by Elmore C. Hall, Education Specialist Extension Course Institute (ATC/ AU) Gunter A FS, Alabama 36118 PREPARED BY SCHOOL OF HEALTH CARE SCIENCES, USAF (ATC) SHEPPARD AIR FORCE BASE, TEXAS EXTENSION COURSE INSTITUTE, GUNTER AIR FORCE STATION, ALABAMA THIS PUBLICATION HAS BEEN REVIEWED AND APPROVED BY COMPETENT PERSONNEL OF THE PREPARING COMMAND IN ACCORDANCE WITH CURRENT DIRECTIVES ON DOCTRINE, POLICY, ESSENTIALITY, PROPRIETY, AND QUALITY. 8 PREFACE MICROBIOLOGY HAS undergone many significant changes within the second half of the twentieth century, during which time tremendous scientific progress has been achieved. Such progressive changes are continuous and require the updating and revising of this course. Thus, in this and in ..!..i$isequent volumes, we have attempted to introduce the latfst applied literature in the field to the bacteriology student and the technologist with the ultimate goal of meeting the professional training of both. This volume enables the student to review the fundamentals of diagnostic bacteriology. The opening chapter briefly traces the historical development of current bacteriological procedures. The second chapter instructs on laboratory techniques and equipment necessary for the safe handling of infectious microorganisms. Chapters 3 and 4 explain how knowledge of bacterial morphology and physiology permits the technician to isolate and cultivate pathogenic microbes in the laboratory. Lastly, Chapter 3 discloses tie basis for antimicrobial sensitivity testing and drug assays. In total, much emphasis has been made upon the ongoing need for and application of quality control principles and techniques. A brief section has been included on quality control guidelines for the disc agar diffusion method of susceptibility testing in relationship to the standardized modified Kirby-Bauer procedure. A glossary is included at the end of this volume to assist you in understanding a number of terms with which you should become familiar. Please note that in this volume we are using the singular pronoun he, his, or him, in its generic sense, not its masculine sense. The word to which it refers person. is If you have questions on the accuracy or currency of the subject matter of this text, or recommendations for its improvement, send them to the School of Health Care Sciences /MSTW, Sheppard AFB TX 76311. NOTE: Do not use the suggestion program to submit corrections for typographical or other errors. If you have questions on course enrollment or administration, or on any of ECI's instructional aids (Your Key to Career Development, Behavioral Objective Exercises, Volume Review Exercise, lnd Course Examination), consult your education or NCO, as training officer, officer, If he can't answer your questions, send them to ECI, Gunter AFS AL 36118, appropriate. preferably on ECI Form 17, Student Request for Assistance. This volume is valued at 63 hours (21 points). Material in this volume is technically accurate, adequate, and curtent as of June 1978. ill D 0 Acknowledgment Figure 2-8 and table 2-3 were furnished through the courtesy of American Sterilizer Company, Erie, Pennsylvania. iv 10