ACADEMIC PRESS RAPID MANUSCRIPT REPRODUCTION Proceedings of an International Conference on Mechanisms in Bioenergetics Sponsored and Supported by the University of Bari and Consiglio Nazionale delle Ricerche, Italy Organized by: G. F. Azzone, L. Ernster, S. Papa, Ε. Quagliariella, and N. Siliprandi Held in Pugnochiuso, Italy May 1-4, 1972 Mechanisms in Bioenergetics Edited by G. F. AZZONE Institute of General Pathology University of Padova Padova, Italy L ERNSTER Institute of Biochemistry University of Stockholm Stockholm, Sweden S. PAPA Department of Biochemistry University of Bari Bari, Italy Ε. QUAGLIARIELLO Department of Biochemistry University of Bari Bari, Italy Ν. SILIPRANDI Institute of Biochemistry University of Padova Padova, Italy ACADEMIC PRESS New York and London 1973 COPYRIGHT © 1973, BY ACADEMIC PRESS, INC. ALL RIGHTS RESERVED. NO PART OF THIS PUBLICATION MAY BE REPRODUCED OR TRANSMITTED IN ANY FORM OR BY ANY MEANS, ELECTRONIC OR MECHANICAL, INCLUDING PHOTOCOPY, RECORDING, OR ANY INFORMATION STORAGE AND RETRIEVAL SYSTEM, WITHOUT PERMISSION IN WRITING FROM THE PUBLISHER. ACADEMIC PRESS, INC. Ill Fifth Avenue, New York, New York 10003 United Kingdom Edition published by ACADEMIC PRESS, INC. (LONDON) LTD. 24/28 Oval Road, London NW1 LIBRARY OF CONGRESS CATALOG CARD NUMBER: 72-9321 PRINTED IN THE UNITED STATES OF AMERICA PARTICIPANTS S. P. J. Albracht, Laboratory of Biochemistry, University of Amsterdam, B. C. P. Jansen Institute, Amsterdam, The Netherlands A. Alexandre, Istituto di Chimica Biologica, Università di Padova, Padova, Italy A. Azzi, Istituto di Patologia Generale, Università di Padova, Padova, Italy G. F. Azzone, Istituto di Patologia Generale, Universita di Padova, Padova, Italy D. Bäckström, Biokemiska Institutionen, Kungl. Universitetet i Stockholm, Stockholm, Sweden E. Balboni, Department of Biological Sciences, Hunter College of the City University of New York, New York, New York M. Baltscheffsky, Botaniska Institutionen, Kungl. Universitetet i Stockholm, Stockholm, Sweden E. Barberio, Istituto di Chimica Biologica, Universita di Bari, Bari, Italy R. E. Basford, Istituto di Patologia Generale, Università di Trieste, Trieste, Italy H. Baum, Department of Biochemistry, Chelsea College of Science and Technology, University of London, London, England R. B. Beechey, Shell Research Ltd., Woodstock Agricultural Research Centre, Sittingbourne, Kent, England M. Bragadin, Istituto di Patologia Generale, Università di Padova, Padova, Italy A. Bruni, Istituto di Farmacologia, Università di Padova, Padova, Italy B. Cannon, Wenner-Grens Institut, Kungl. Universitetet i Stockholm, Stockholm, Sweden xi PARTICIPANTS Ε. Carafoli, Istituto di Patologia Generale, Università di Modena, Modena, Italy F. Cedrangolo, Istituto di Chimica Biologica, Università di Napoli, Napoli, Italy Britton Chance, Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania A. Cittadini, Istituto di Patologia Generale, Università Cattolica del S. Cuore, Roma, Italy R. Colonna, Istituto di Patologia Generale, Università di Padova, Padova, Italy A. R. Crofts, Department of Biochemistry, University of Bristol, Bristol, England R. L. Cross, Molecular Biology Institute, University of California, Los Angeles, California B. de Bernard, Istituto di Chimica Biologica, Università di Trieste, Trieste, Italy P. Dell'Antone, Istituto di Patologia Generale, Università di Padova, Padova, Italy A. De Montalvo, Istituto di Chimica Biologica, Università di Bari, Bari, Italy P. Leslie Dutton, Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania Joe A. Elder, Department of Physiological Chemistry, The Johns Hopkins University, School of Medicine, Baltimore, Maryland K. Enander, Biokemiska Institutionen, Kungl. Universitetet i Stockholm, Stockholm, Sweden Lars Ernster, Biokemiska Institutionen, Kungl. Universitetet i Stockholm, Stockholm, Sweden A. Fonyo, Experimental Research Department, Medical University of Budapest, Budapest, Hungary L. Frigeri, Istituto di Patologia Generale, Università di Padova, Padova, Italy xii PARTICIPANTS T. Galeotti, Istituto di Patologia Generale, Università Cattolica del Sacro Cuore, Roma, Italy D. C. Gautheron, Laboratoire de Chimie Biologique, Université Claude Bernard de Lyon, Lyon, France P. Gazzotti, Istituto de Patologia Generale, Università di Modena, Modena, Italy G. Genchi, Istituto di Chimica Biologica, Università di Bari, Bari, Italy G. V. Gnoni, Istituto di Chimica Biologica, Università di Bari, Bari, Italy M. Greco, Istituto di Chimica Biologica, Università di Bari, Bari, Italy C. Grimmelikhuijzen, B. C. P. Jansen Institute, University of Amsterdam, Amsterdam, The Netherlands F. Guerrieri, Istituto di Chimica Biologica, Università di Bari, Bari, Italy T. Gulik, Centre de Génétique Moléculaire, C. Ν. R. S., Gif-sur-Yvette, France M. Gutman, Tel-Aviv University, Tel-Aviv, Israel D. Gutnick, Department of Microbiology, Tel-Aviv University, Tel-Aviv, Israel Charles R. Hackenbrock, Department of Cell Biology, The University of Texas Southwestern Medical School at Dallas, Dallas, Texas W. Hasselbach, Max-Planck-Institut fur Physiologie, Heidelberg, Germany H. W. Heidt, Institut für Physiologische Chemie und Physikalische Biochemie, der Universität München, München, Germany P. C. Hinkle, Division of Biological Sciences, Section of Biochemistry and Molecular Biology, Cornell University, Ithaca, New York K. Juntti, Biokemiska Institutionen, Kungl. Universitetet i Stockholm, Stockholm, Sweden D. Kanduc, Istituto di Chimica Biologica, Università di Bari, Bari, Italy A. Kemp, B. C. P. Jansen Institute, University of Amsterdam, Amsterdam, The Netherlands A. Kepes, Service de Biochimie Cellulaire, Institut Pasteur, Paris, France xiii PARTICIPANTS M. Klingenberg, Institut für Physiologische Chemie und Physikalische Biochemie der Universität München, München, Germany Α. Kröger, Institut für Physiologische Chemie und Physikalische Biochemie der Universität München, München, Germany C. Landriscina, Istituto di Chimica Biologica, Università di Bari, Bari, Italy C. P. Lee, Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania Albert L. Lehninger, Department of Physiological Chemistry, The Johns Hopkins University, School of Medicine, Baltimore, Maryland G. Lenaz, Istituto di Chimica Biologica, Università di Bologna, Bologna, Italy Ν. E. Lofrumento, Istituto di Chimica Biologica, Università di Bari, Bari, Italy M. Lorusso, Istituto di Chimica Biologica, Università di Bari, Bari, Italy G. Loschen, Istituto di Patologia Generale, Università di Padova, Padova, Italy S. Luciani, Istituto di Farmacologia, Università di Padova, Padova, Italy V. Luzzati, Centre de Génétique Moléculaire, C.N.R.S., Gif-sur-Yvette, France L. Masotti, Istituto di Chimica Biologica, Università di Bologna, Bologna, Italy S. Massari, Istituto di Patologia Generale, Università di Padova, Padova, Italy A. Baccarini Melandri, Istituto ed Orto Botanico, Università di Bologna, Bologna, Italy B. A. Melandri, Istituto ed Orto Botanico, Università di Bologna, Bologna, Italy J. Meyer, Laboratoire de Biochimie, Centre d'Etudes Nucléaires, Faculté de Médecine, Université de Grenoble, Grenoble, France J. Michejda, Department of Biochemistry, Institute of Biology, Poznan University, Poznan, Poland P. Mitchell, Glynn Research Laboratories, Bodmin, Cornwall, England V. Moret, Istituto di Chimica Biologica, Università di Padova, Padova, Italy xiv PARTICIPANTS D. G. Nicholls, Wenner-Grens Institut, Kungl. Universitetet i Stockholm, Stockholm, Sweden Peter Nicholls, Biophysics Unit, Agricultural Research Council, Institute of Animal Physiology, Babraham, Cambridge, England Kerstin Nordenbrand, Biokemiska Institutionen, Kungl. Universitetet i Stockholm, Stockholm, Sweden Birgitta Norling, Biokemiska Institutionen, Kungl. Universitetet i Stockholm, Stockholm, Sweden Κ. Ohlson, Biokemiska Institutionen, Kungl. Universitetet i Stockholm, Stockholm, Sweden Lester Packer, Department of Physiology, University of California, Berkeley, California F. Palmieri, Istituto di Chimica Biologica, Università di Bari, Bari, Italy S. Papa, Istituto di Chimica Biologica, Università di Bari, Bari, Italy G. Paradies, Istituto di Chimica Biologica, Università di Bari, Bari, Italy S. Passarella, Istituto di Chimica Biologica, Università di Bari, Bari, Italy Robert L. Post, Department of Physiology, Vanderbilt Univerity, Nashville, Tennessee B. C. Pressman, Department of Pharmacology, School of Medicine, University of Miami, Miami, Florida Ernesto Quagliariello, Istituto di Chimica Biologica, Università di Bari, Bari, Italy M. P. Roisin, Institut de Biologie Moléculaire, Faculté des Sciences, C. N. R. S., Paris, France J. Rosing, B. C. P. Jansen Institute, University of Amsterdam, Amsterdam, The Netherlands C. R. Rossi, Istituto di Chimica Biologica, Università di Padova, Padova, Italy C. S. Rossi, Istituto di Chimica Biologica, Università di Padova, Padova, Italy xv PARTICIPANTS Jan Rydström, Biokemiska Institutionen, Kungl. Universitetet i Stockholm, Stockholm, Sweden C. Saccone, Istituto di Chimica Biologica, Università di Bari, Bari, Italy D. Rao Sanadi, Boston Biomedical Research Institute, Boston, Massachusetts G. Schäfer, Institut für Klinische Biochemie und Physiologische Chemie, Hannover-Kleefeld, Germany G. Semenza, Departments of Pediatrics, Microbiology and Biochemistry of the University of Zürich, Zürich, Switzerland Philip Siekevitz, The Rockfeller University, New York, New York N. Siliprandi, Istituto di Chimica Biologica, Università di Padova, Padova, Italy S. Simone, Istituto di Chimica Biologica, Università di Bari, Bari, Italy Thomas P. Singer, Molecular Biology Division, Veterans Administration Hospital, San Francisco, California E. C. Slater, B. C. P. Jansen Institute, University of Amsterdam, Amsterdam, The Netherlands C. Smith, Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania G. L. Sottocasa, Istituto di Chimica Biologica, Università di Trieste, Trieste, Italy Β. T. Storey, Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania Patrik Swanljung, Biokemiska Institutionen, Kungl. Universitetet i Stockholm, Stockholm, Sweden T. Terranova, Istituto di Patologia Generale, Università Cattolica del S. Cuore, Roma, Italy I. Vallin, Wenner-Grens Institut, Kungl. Universitetet i Stockholm, Stockholm, Sweden K. Van Dam, B. C. P. Jansen Institute, University of Amsterdam, Amsterdam, The Netherlands xvi PARTICIPANTS R. J. van de Stadt, Β. C. P. Jansen Institute, University of Amsterdam, Amsterdam, The Netherlands B. F. Van Gelder, Β. C. P. Jansen Institute, University of Amsterdam, Amsterdam, The Netherlands P. M. Vignais, Laboratoire de Biochimie, Centre d'Etudes Nucléaires et Faculté de Médecine, Universite de Grenoble, Grenoble, France P. V. Vignais, Laboratoire de Biochimie, Centre d'Etudes Nucléaires et Faculté de Médecine, Universite de Grenoble, Grenoble, France G. von Jagow, Institut für Physiologische Chemie und Physikalische Biochemie der Universität München, München, Germany L. Wheeldon, Laboratoire de Biochimie, Centre d'Etudes Nucléaires et Faculté de Medicine, Universite de Grenoble, Grenoble, France Marten Κ. F. Wikström, B. C. P. Jansen Institute, University of Amsterdam, Amsterdam, The Netherlands David F. Wilson, Johnson Research Foundation, University of Pennsylvania, Philadelphia, Pennsylvania F. Zanotti, Istituto di Chimica Biologica, Università di Bari, Bari, Italy xvii