Leukocyte Typing II VOLUME 1 Human T Lymphocytes Leukocyte Typing II Volume 1 Human T Lymphocytes Volume 2 Human B Lymphocytes Volume 3 Human Myeloid and Hematopoietic Cells Leukocyte Typing II VOLUME 1 Human T Lymphocytes Edited by Ellis L. Reinherz Barton F. Haynes Lee M. Nadler Irwin D. Bernstein With 123 Illustrations Springer-Verlag New York Berlin Heidelberg Tokyo EUis L. Reinherz, M.D., Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 021I5 U.S.A. Barton F. Haynes, M.D., Department of Medicine, Duke University School of Medicine, Durham, NC 27710 U.S.A. Lee M. Nadler, M.D., Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA 02115 U.S.A. Irwin D. Bernstein, M.D., Program in Pediatric Oncology, Fred Hutchinson Can cer Research Center, Seattle, WA 98104 U.S.A. Library of Congress Cataloging in Publication Data Main entry under title: Leukocyte typing II. Papers presented at the Second International Workshop on Human Leukocyte Differentiation Antigens, held in Boston, Sept. 17-20, 1984. Includes bibliographies and indexes. Contents: v. 1. Human T lymphocytes-v. 2. Human B lymphocytes-v. 3. Human myeloid and hematopoietic cells. 1. Leucocytes-Classification-Congresses. 2. Histocompatibility testing-Congresses. 3. Tissue specific antigens-Analysis-Congresses. I. Reinherz, Ellis L. II. International Workshop on Human Leukocyte Differentiation Antigens (2nd: 1984 : Boston, Mass.) III. Title: Leukocyte typing 2. IV. Title: Leukocyte typing two. QRI85.8.L48L48 1985 616.07'9 85-22229 © 1986 by Springer-Verlag New York Inc. Softcover reprint of the hardcover 1st edition 1986 All rights reserved. No part of this book may be translated or reproduced in any form without written permission from Springer-Verlag, 175 Fifth Avenue, New York, New York 10010, U.S.A. The use of general descriptive names, trade names, trademarks, etc., in this pUblication, even if the former are not especially identified, is not to be taken as a sign that such names, as understood by the Trade Marks and Merchandise Marks Act, may accordingly be used freely by anyone. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Permission to photocopy for internal or personal use, or the internal or personal use of specific clients, is granted by Springer-Verlag New York Inc. for libraries registered with the Copyright Clearance Center (CCC), provided that the base fee of $0.00 per copy, plus $0.20 per page is paid directly to CCC, 21 Congress Street, Salem, MA 01970, U.S.A. Special requests should be addressed directly to Springer-Verlag New York, 175 Fifth Avenue, New York, New York tooto, U.S.A. ISBN-13:978-I-46I3-8589-9/1986 $0.00 + .20 Typeset by Bi-Comp Inc., York, Pennsylvania. 9 8 7 6 5 432 1 ISBN -13: 978-1-4613-8589-9 e-ISBN -13: 978-1-4613-8587-5 DOl: 10.1007/978-1-4613-8587-5 Preface The Second International Workshop on Human Leukocyte Differentia tion Antigens was held in Boston, September 17-20, 1984. More than 350 people interested in leukocyte differentiation agreed to exchange reagents and participate in this joint venture. All in all, in excess of 400 antibodies directed against surface structures on T lymphocytes, B lymphocytes, and myeloid-hematopoietic stem cells were characterized. Because of the enormous quantity of serologic, biochemical, and functional data, Leuko cyte Typing II has been divided into three volumes. These books represent the written results of workshop participants. They should be helpful to both researchers and clinicians involved in scientific endeavors dealing with these broad fields of immunobiology. To those who delve into the various sections of the volumes, it will become evident that the work speaks for itself. I am deeply indebted to the section editors, Barton F. Haynes, Volume 1, Human T Lymphocytes, Lee M. Nadler, Volume 2, Human B Lympho cytes, and Irwin D. Bernstein, Volume 3, Human Myeloid and Hemato poietic Cells for their major contributions in planning, executing, and summarizing the workshop, as well as council members John Hansen, Alain Bernard, Laurence Boumsell, Walter Knapp, Andrew McMichael, Cesar Milstein, and Stuart F. Schlossman. I would also like to thank the National Institutes of Health, World Health Organization, and Interna tional Union of Immunological Societies for making this meeting possible. Needless to say, I am most grateful to all of my colleagues who contrib uted to this effort and helped to accelerate the characterization of human immunobiology through their endeavors. Ellis L. Reinherz. M.D. Contents I. Introduction 1. Summary of T Cell Studies Performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens B.F. Haynes 3 II. Functional Studies 2. Binding and Functional Analysis of the Workshop T Cell Specific Set of Monoclonal Antibodies F.M. Gotch, N. Carter, S. Ellis, P. McShane, 1. Heyman, M. Palmer, D. W. Dongworth, A. Bewick, P.l. Morris, A.l. McMichael 31 3. Functional Studies Performed with the Second Interna tional Workshop T Cell Panel of Monoclonal Antibodies D. Charmot, D. Olive, P. Dubreuil, M. Ragueneau, C. Mawas 45 4. The Epitopic Dissection of the CD2 Defined Molecule: Relationship of the Second Workshop Antibodies in Terms of Reactivities with Leukocytes, Rosette Blocking Proper ties, Induction of Positive Modulation of the Molecule, and Triggering T Cell Activation A. Bernard, P. Brottier, E. Georget, V. Lepage, L. Boumsell 53 5. Monoclonal Antibodies as Probes to Define Critical Surface Structures Involved in T Cell Activation S.C. Meuer, T.H. Hutteroth, K.H. Meyer zum Biischenfelde 67 6. The Development of Monoclonal Antibodies against Human Immunoregulatory T Cell Subsets: The Isolation of viii Contents Human Suppressor Inducer T Cell Subset C. Morimoto, N.L. Letvin, S.F. Schlossman 79 7. Lectin Activation Induces T4, T8 Coexpression on Peripheral Blood T Cells M.L. Blue, I.F. Daley, H. Levine, S.F. Schlossman 89 8. Analysis of the Ontogeny and Function of Human Helper T Cell Subpopulations L.T. Clement, M.D. Cooper, T.F. Tedder 101 9. Effects of Workshop Monoclonal Antibodies on the Cytolytic Activity of Alloreactive CD4-Positive T Cell Clones N. Flomenberg, B. Dupont, R. W. Knowles 111 10. Effects of the Workshop Anti-T Cell Monoclonal Antibod ies on Lymphocyte Proliferative and Cytotoxic Func- tions G. Gorog, G. Batory, T. Laskay, G.G. Petranyi 123 11. T Cell Activation by CD3 Antibodies R. Kurrle, W. Seyfert, A. Trautwein, F.R. Seiler 137 12. Studies of T Cell Proliferation Induced by Monoclonal Antibodies of the Second International Workshop P.J. Martin, I.A. Ledbetter, P.G. Beatty, E.A. Clarke, I.A. Hansen 147 13. Phorbol Ester Induces Changes in the Pattern of Cell Surface Molecules Involved in CTL-Target Cell Interaction I. Ando, D.H. Crawford, P.c.L. Beverley 157 14. Analysis of the Monocyte-T Cell Interaction Required for Induction of T Cell Proliferation by Anti-T3 Antibodies L.T. Clement 163 15. Evaluation of the T Cell Workshop Monoclonal Antibodies in In Vitro Lymphocyte Proliferation Assays N.L. von leney, K. Olas, W. Knapp 173 16. The Influence of the Workshop Monoclonal Antibodies on CML, AgTR, PLT, ADCC, and NK Cell Activity. Func tional Studies with Workshop Antibodies F. Koning, M. Kardol, I. van der Poel, A. Termijtelen, E. Goulmy, T. Ottenhoff, E. Blokland, D. Elferink, I. Pool, S. Naipal-van den Berge, H. Bruning 189 17. The Influence of Anti-T Cell Monoclonal Antibodies on Calcium Mobilization: Investigation of Workshop Antibod- ies K. O'Flynn, P.c.L. Beverley, D.C. Linch 205 Contents ix 18. T Cell-Dependent Immunoglobulin Synthesis in the Human System. Studies with T Cell-Specific Monoclonal Antibodies F. Miedema, F.G. Terpstra, C.J.M. Melief 213 19. Monoclonal Antibodies Recognizing the T3/Leu4 T Cell Differentiation Antigen Induce Suppressor Cells J.E. Kunicka, C.D. Platsoucas 223 20. Modulation of T Cell Functions by Monoclonal "Pan T Cell" Antibodies Not Directed against the T Cell Receptor Complex P. Rieber, G. Rank, S. Wirth, M. Wilhelm, E. Kopp, G. Riethmuller 233 21. Determinant Heterogeneity of CD5, CD8, and CD4 Anti gen Molecules As Defined by Monoclonal Antibodies T. Takei, Y. Ishii, S. Kon, J. Fujimoto, K. Kikuchi 243 III. Biochemical Studies 22. Immunochemical Analysis of the T Cell-Specific Antigens R. W. Knowles 259 23. Epitopic Groups of CD1 Molecules L. Boumsell, M. Amiot, B. Raynal, V. Gay-Belli/e, B. Ca illou , A. Bernard 289 24. Comparison of the CD7 (3Al) Group of T Cell Workshop Antibodies T.J. Palker, R.M. Scearce, L.L. Hensley, W. Ho, B.F. Haynes 303 IV. Phenotypic Characterization of T Cell Leukemias and Lymphomas 25. Study on Human T Leukemia-Lymphoma Cell Lines by the Second International Workshop Monoclonal Antibod ies of the T Cell Protocol J. Minowada, M. Menon, H.G. Drexler, S. Gignac, B. Misra, L. Skowron 317 26. Expression of T Cell-Related Antigens on Cells from the Myelo-Monocytic Lineage P. Dubreuil, P. Mannoni, D. Olive, B. Winkler-Lowen, C. Mawas 335 27. Phenotype of Hodgkin Cells. Expression of the Tac Antigen and Other Determinants K.J. Bross, R. Andreesen, T. Monnheimer, J. Osterholz, G. W. Lohr 343 28. The Diversity of T Cell Activation Antigens. Serological Analysis Including Their Expression on Non-T Acute x Contents Lymphoblastic Leukemia Cells and B Cell Lines Derived from Adult T Cell Leukemia Patients Y. Morishima, T. Noumi, K. Ohya, S. Mimami, M. Okumura, S. Mizuno, R. Ohno, K. Miyamoto 351 29. Expression Profiles of T Cell Associated Antigens on Adult T Cell Leukemia Cells: Results of a Workshop Study K. Sagawa, S. Hagiwara, K. Yasuda, M.M. Yokoyama 361 V. Phylogenetic Studies Using Anti-T Cell Antibodies 30. The Phylogeny of T Cell Antigens M. Jonker, F.J.M. Nooij 373 31. Conserved T Lymphocyte-Specific Antigens in Primates N.L. Letvin 389 VI. Study of Anti-T Cell Antibodies on Frozen Tissue Sections of Human Tissue 32. Ontogeny of Human T Cell Antigens D.F. Lobach, L.L. Hensley, W. Ho, B.F. Haynes 397 33. Cross-reactivity of Anti-lymphocyte Monoclonal Antibod- ies on Human Skin Components J. Brochier, D. Schmitt 407 VII. Study of Antibodies Reactive with Antigens of Activated T Cells 34. Flow Cytometric Analysis of the Antigens Expressed by Peripheral Blood Lymphocytes Stimulated by Phyto hemagglutinin Using the Workshop T Cell Panel and Selected Band M Cell Panel Monoclonal Antibodies D. Olive, P. Dubreuil, C. Mawas, P. Mannoni 417 35. Regulation of Activation and Proliferation in T Cells P.C.L. Beverley, K. O'Flynn, D.L. Wallace, J.R. Lamb, A. W. Boylston, D.C. Linch 427 36. Phenotypic Analysis of Activation Antigens on Mitogen stimulated T Cells Utilizing Monoclonal Antibodies T. Umiel, E. Saltz-Tal 441 37. T-Activation Antigens: Kinetics of Appearance and Effect on Cell Proliferation Studied with Monoclonal Antibodies L. Del Vecchio, M. De Felice, M.C. Turco, M. Maio, E. Pace, C. Lo Pardo, C. Vacca, S. Venuta, S. Zappacosta 453 Contents xi VIII. Study of T Cell Leukemia-Associated Antigens 38. Antibody 3-40 Binds to Keratin and Vimentin Intermediate Filaments A.J. Laster, T.J. Palker, E.A. Harden, W. Ho, K. Naito, B. DuPont, B.F. Haynes 465 IX. Other Types of Studies and Description of New T Cell Markers 39. Screening of Monoclonal Antibodies against T Cell Differ- entiation Antigens Using Transfectants or Somatic Cell Hybrids R. Ueda, N. Emi, I. Tsuge, K. Nishida, K.R. Utsumi, S. Takamoto, T. Maruyama, K. Ota, T. Takahashi 477 40. Association of IL-2 Production with the Expression of Dipeptidyl Peptidase IV (DPIV) on Human T Lympho- cytes W. Scholz, A.C. Feller, A.J. Ulmer, H.D. Flad 489 41. A Novel Disulfide-Linked Cell Surface Molecule Present on Resting and Activated Human T Lymphocytes R.D. Bigler, N. Chiorazzi 503 42. T14, A Non-modulating 150-Kd T Cell Surface Antigen H. Stockinger, O. Majdic, K. Liszka, U. Koller, W. Holter, C. Peschel, P. Bettelheim, H. Gisslinger, W. Knapp 513 43. Specific Membrane Lectin of Human T Suppressor Cells C. Kieda, M. Monsigny 531 Index 537