ABSTRACTS – Late Breaking LATE BREAKING Discussion: Ectopic ACTH cushings syndrome from breast cancer is extremly rare presenting with significant morbidity Abstract # 1300 & mortality from opportunistic infections, psychoses, metabolic & coagulation derangements. This case, reports CUSHING’S STORM SECONDARY TO ECTOPIC management of cushings psychosis resulting from PRES ACTH SECRETING METASTATIC BREAST & hypertension with etomidate followed by metyrapone CANCER & spironlactone transition since mifepristone could not be increased in liver dysfunction. Maura Bucciarelli, Ya-Yu Lee, Vasudev Magaji Conclusion: Ectopic cushings syndrome presents unique diagnostic & management challenges. Lehigh Valley Hospital, Allentown, PA, United States. Abstract # 1301 Adrenal Disorders UNIQUE DIAGNOSTIC CHALLENGES OF Objective: Describe a rare ectopic acth secreting breast CUSHINGS FROM LARGE BILATERAL ADRENAL cancer causing cushings syndrome presenting with ADENOMA AND MIFEPRISTONE UTILIZATION posterior reversible encephalopathy syndrome (PRES) from FOR HYPERCORTISOLISM PRIOR TO SURGERY. uncontrolled hypertension leading to psychosis along with opportunistic infection & liver function abnormalities posing Vasudev Govardhan Magaji, Sarah Park, Maura Bucciarelli, unique management issues. Katie L. Mastoris, Scott Beman Case Presentation: A 31 year old female, with triple negative, high grade invasive right breast ductal carcinoma Lehigh Valley Hospital treated with chemotherapy, bilateral mastectomy & radiation presented with acute psychosis. She had no sleep for 4 days, Adrenal Disorders was hyperenergetic, easily distracted & impulsive with racing thoughts, pressured speech & was paranoid that her husband Objective: Describe evaluation & management of Cushings was trying to hurt her. On exam she had round, ruddy hirsuite patient with bilateral (b/l) adrenal adenomas. face with acne & BP 156/108. Labs showed K 1.7, random Case Presentation: A 47 year old female was found to have a cortisol >70mcg/dL, 1mg & 8mg overnight dexamethasone 1.8 x 4.9 cm right adrenal nodule & 2.8 x 4.3 cm left adrenal showed cortisol >100mcg/dL. She had elevations in AST nodule with calcification on CT during abdominal pain 103, ALT 237, ACTH 1173 pg/ml, Total testosterone 170ng/ evaluation. She had hypokalemia of 6months, uncontrolled dL, DHEA-S 499mcg/dL, 17 OH progesterone 1780 ng/dL & hypertension despite metoprolol & lisinopril treatment, 24 hr urine cortisol (UFC) 14766mcg. CT abdomen showed glucose intolerance, stroke, peripheral vascular disease, extensive hepatic metastatic disease & bilateral adrenal CABG & chronic cellulitis at vien harvest site. She had hyperplasia. Renin, aldosterone, plasma metanephrines, high, urine free cortisol 89.2g/24hr, cortisol on 1mg & chromagranin A, corticotropin releasing hormone & gastrin 8mg overnight dexamethasone testing was 18.4 & 20 μg/ levels were normal. Imaging was -ve for thyroid nodule, dL respectively, 11pm salivary cortisol X2, with random thymic neoplasm & bronchial carcinoid. Core liver biopsy cortisol 17.3 μg/dL & ACTH < 5 pg/mL and normal revealed metastatic breast adenocarcinoma that was -ve DHEA-S, plasma metanephrine, androsteindione, renin & for neuro endocrine markers CD56, synaptophysin, neuron aldosterone levels. Since adenomas were > 4cms b/l adrenal specific enolase & chromogranin. CT head showed white surgery was indicated. Adrenal venous sampling (AVS) matter disease consistent with PRES. She was psychotic done with dexamethasone 2 mg PO Q6hours X 1 day, for & hyperensive despite using mifepristone with multiple nodule functional assessment, showed left cortisol 84.9 antihypertensives including lisinopril, aldactone & metoprolol epinephrine 433 pg/mL, right cortisol 56.5, epinephrine 1911 targeting a systolic BP 110-130. Transaminitis did not allow and venacaval cortisol 11.7 and epinephrine 28. Adrenal vein mifepristone escalation > 600mg/day. Etomidate infusion at epinephrine levels stepup >100pg/mL indicated succesful non sedating dose of 0.1mg/kg/min in ICU controlled her catheterization, b/l adrenal vein to vencaval cortisol ratio >4 hypertension & cortisol levels to 20-30mcg/dL. UFC reduced was consistent with autonomous cortisol secretion & left to to 820mcg. She was transitioned to metyrapone 1250mg right adrenal cortisol ratio < 2 suggested b/l cushings based PO Q 6h & spironolactone 100mg PO Q 6h. Dapsone used on literature from Mayo Clinic. First step of right adrenal for PCP prophylaxis due to bactrim allergy did not prevent surgery was defferred for her carotid artery surgery. Also her PCP pneumonia, which was treated primaquine. She was multiple co-morbidities, hypertension, poor functional status discharged from hospital on metyrapone with spironolactone needing walker & cane, chronic cellulits & glucose intolerance & is undergoing chemotherapy. required medical management of hypercortisolism prior to - 207 - ABSTRACTS – Late Breaking surgery. Ketoconazole was contraindicated since she was high likelihood of mortality (OR 50, P =< 0.001). Among on plavix & atorvastatin. Mifepristone was started after those who survived, inotropic support was required for her carotid surgery. She had, 50lbs weight loss, normal BP, longer period in relative as compared to absolute AI and complete healing of chronic leg cellulitis of 10months on to non-AI. antibiotics & could ambulate independently by 4 months Discussion: We have observed that patients with AI of Mifepristone. Her potassium, BP & liver functions required vasopressors support for longer time than who tests were closely monitored. Her right adrenal adenoma did not. Only the patients with absolute AI insufficiency resection on pathology had multiple adrenocortical and patients of non-AI with high level of serum cortisol nodules without adrenal carcinoma. (> 1210 nmol/L) had shown the response to vasopressor Discussion: This case had unique diagnostic & therapy. But, there was no response in patients with management dilemmas. Aldosterone in AVS, reportedly relative AI in septic shock. Though the study has one used in case reports, might have helped in diagnosis, given important limitation that it comprised of small number of the subtly low right cortisol & low left epinephrine levels subjects, we would like to conclude that AI was prevalent (likely from phrenic vein dilution) but based on size, among patients with septic shock, and it was related to b/l adrenal resection was indicated. Mifepristone, FDA prolonged requirement of vasopressors and to prolonged approved for inoperable Cushing, facilitated pre surgery duration of shock. In septic shock, despite treatment with optimization by mitigating hypercortisolism. hydrocortisone in patients with AI, these patients had Conclusion: Bilateral adrenal tumours in Cushings pose higher mortality compared to those who did not have AI, complex clinical problems. Mifepristone could potentially and the higher mortality risk was most likely with absolute be utilized as a bridge when surgery needs to be defferred. AI. The mortality risk was also high when baseline serum cortisol was more than 1210 nmol/L. We would like to Abstract # 1302 recommend evaluating the adrenal status in patients with septic shock because it would entail prognosis. INCIDENCE OF ADRENAL INSUFFICIENCY AND Conclusion: AI is prevalent among patients with septic ITS RELATION TO MORTALITY IN PATIENTS shock. We found that higher APACHE scores were WITH SEPTIC SHOCK associated with higher rates of adrenal failure and mortality in patients with septic shock. There also appears Manish Gutch, Sanjay Saran, Manish Gutch to be a bimodal distribution of mortality with adrenal status in patients with septic shock. Endocrinology and Metabolism, LLRM Medical College, Meerut, U.P, India Abstract # 1303 Adrenal Disorders PROGNOSTIC FACTORS IN PATIENTS HOSPITALISED WITH DIABETIC Objective: To determine the incidence of adrenal KETOACIDOSIS insufficiency and its relation to mortality in patients with septic shock. Manish Gutch1, Ambuj Yadav2, Keshav K. Gupta1, Methods: In patients of septic shock, APACHE II score Sanjay Saran1, Avinash Agarwal1, Syed M. Razi1 was calculated and serum cortisol was measured at the time of admission and 1 hour after giving 250 μg ACTH. 1. Endocrinology And Metabolism, LLRM Medical Hydrocortisone was added to inotropics in all patients College, Meerut, U.P, India. 2. King George’s Medical after drawing 2nd blood sample for serum cortisol and was University, Lucknow, U.P, India continued till 7 days or less. In our study, the patients with inadequate adrenal response were dividedinto two groups: Diabetes/Prediabetes 1) absolute adrenal insufficiency – baseline cortisol < 20 µg/dL and increment ≤ 9 µg/dL after the ACTH stimulation Objective: 1. To evaluate the clinical and biochemical test; 2) relative adrenal insufficiency – patients with baseline prognostic markers in diabetic ketoacidosis. 2. To correlate cortisol≥20 µg/dL and increment≤9 µg/dL. the various prognostic markers with mortality in diabetic Results: The incidence of AI in septic shock was 42% ketoacidosis. (absolute 14%, relative 28%). The mortality rate was Methods: Two hundred and seventy patients hospitalized 48%, and it was higher in patients with AI than in patients with diabetic ketoacidosis over a period of 1 year were without AI (P = 0.017). The APACHE II score > 25 carried evaluated clinically and by laboratory tests. Serial assays higher mortality rate than a score of < 25 (P =< 0.001). of serum electrolytes, glucose and blood pH, and clinical Baseline serum cortisol > 1210 nmol/L had exceptionally outcome of either discharge home or death were evaluated. - 208 - ABSTRACTS – Late Breaking Results: The significant predicators of final outcome –0.12+1.3 at the start of gluten free diet to 0.8+0.9 after obtained were further regressed together and subjected 12 months later (p<0.05). Height SDS increased from with multivariate logistic regression (MLR) analysis. The –2.46+1.1 at the start of gluten free diet to –2.14+0.9 after MLR analysis further revealed that the male sex had 7.93 12 months later (p =0.087). Bone age SDS increased from fold higher favorable outcome as compared to female 9.2+6.3 at the start of gluten free diet to 10.3+6.7 after sex (OR=7.93, 95% CI=3.99-13.51) while decrease in 12 months later. Height velocity increased from 4.7+0.7 mean APACHE II score (14.83) and S. PO3-- (4.38) at cm/year in the year before treatment to 5.1+1.2during presentation may lead 2.86 ( OR=2.86, 95% CI=1.72- treatment (p= 0.05). The increased in Hemoglobin, 7.03) and 2.71 (OR=2.71, 95% CI=1.51-6.99) fold better serum calcium, and serum iron is statistically significant favourable outcome respectively as compared to higher (p<0.05). levels (APACHE II score: 25.00; S. PO3--: 6.04). Discussion: There are very few studies which show Discussion: Sex, baseline biochemical parameters like the effect of gluten free diet on weight, height, height APACHE II Score, and phosphate level, were important velocity, and other biochemical parameters in celiac predicator of mortality from DKA. type 1 diabetes patients. There is very limited data from Conclusion: Diabetic ketoacidosis still remains a big the Indian subcontinent. Numerous studies showed the threat to the emergency department. Prompt diagnosis positive effect of Gluten-free diet on increased weight, and intervention can reduced the morbidity and mortality height and growth velocity as well as on serum calcium, associated with diabetic ketoacidosis. serum ferritin and hemoglobin. Moreover improvement of bone status in patients with type 1 diabetes mellitus and Abstract # 1304 adherence to gluten-free diet has been reported. Conclusion: Celiac disease was found to be significantly PREVALENCE, CLINICAL PROFILE, AND associated with type 1 diabetes, timely identification of GLYCEMIC VARIABILITY AND EFFECT these disorder are of paramount important for better OF GLUTEN FREE DIET ON WEIGHT AND glycemic control and to reduced the morbidity and GROWTH VELOCITY IN CELIAC DISEASE mortality associated with the conditions. PATIENTS WITH TYPE 1 DIABETES MELLITUS IN WESTERN, U.P, INDIA Abstract # 1305 Manish Gutch1, Sukriti Kumar2, Keshav K. Gupta1, SENTARA HEALTH SYSTEM SEES GLYCEMIC Sanjay Saran1, Syed M. Razi1 IMPROVEMENTS FOR HOSPITAL BASAL BOLUS PATIENTS USING E-GLYCEMIC 1. Endocrinology And Metabolism, LLRM Medical MANAGEMENT SYSTEM College, Meerut, U.P, India. 2. SGPGI, Lucknow, U.P, India. Joseph Aloi1, Raymie McFarland3, Paul Chidester2 Diabetes/Prediabetes 1. Eastern Virginia Medical School, Norfolk, VA, United States. 2. Sentara Healthcare, Norfolk, VA, United Objective: To study the prevalence, clinical profile and States. 3. Glytec, Greenville, SC, United States. glycemic variability and the effect of gluten free diet on growth and diabetic control in celiac type 1 diabetes Diabetes/Prediabetes patients in a tertiary care referral centre in north India. Methods: Total of two hundred and fifty six patients were Objective: Hospitalized patients who experience screened (149 males and 107 females) during the study hypoglycemia, whether insulin induced or spontaneous, period of two years, patients were evaluated for the clinical have been associated with increased mortality and signs, biochemical investigations and family history of hospital cost as compared with patients with an absence celiac disease in tertiary care health center in western U.P. of hypoglycemia. Sentara’s successful experience with Results: Twenty four (9.37%) patients were diagnosed to Glucomander ®, (GM) a web based insulin algorithm have celiac disease; the mean age at diagnosis of diabetes integrated with the hospital EHR, at Virginia Beach Hospital was 9.34 ± 7.3 years. Only 1/24 patients with celiac in inpatient insulin protocols suggested that its expanded use disease had been diagnosed before detection of diabetes across the Sentara Health System may improve glycemic mellitus. The common manifestations were normocytic control for Subcutanesously (SQ) treated patients with normochromic anemia (66.6%) followed by diarrhoea Basal Bolus Insulin (BBI) Orders. We studied the glycemic (62.5%), abdominal pain/bloating sensation (58.3%), impact using the GM system for the treatment patients with and short stature (58.3%). Weight SDS increased from hyperglycemia compared to BBI therapy alone. - 209 - ABSTRACTS – Late Breaking Methods: This 30-day quality initiative is being conducted pay for performance; a greater emphasis will be placed at Sentara Healthcare System in southeast Virginia across on institutions to achieve nationally standardized 2 Hospital sites. Patients needing SubQ insulin were glucometrics. Glucommander (GM) was previously placed on the GM system and we measured the first 30 proven to improve throughput effectiveness for the days of use to the hypoglycemia baseline of the 30 days treatment of mild to moderate Diabetic Ketoacidosis prior to GM at each site. Electronic BBI order sets were (DKA) in the Emergency Department. This study is used to treat insulin patients during the baseline period. focused on safely improving length of stay (LOS) for the The glycemic targets being used for this project were % of diabetic patients with DKA utilizing real-time clinical blood glucose events <70 mg/dl and Glucose Profile Day surveillance, GlucoSurveillanceTM. 1 vs Day of Discharge for the 178 patients in this analysis. Methods: We reviewed the hospital care of 168 patients Results/Case Presentation: Baseline hypoglycemia episodes with a diagnosis of DKA at 3 community hospitals. (<70 mg/dl) were reduced by 56% with GM SQ compared The patients were studied over a 3-month time period. to BBI. GM SQ had a Day1 to Day of Discharge delta Qualifying patients had a diagnosis code of DKA (ICD of 70 mg/dl from 233 to 163 (mg/dl) for a 30% reduction 150.10-250.13), elevated admission glucose (>250 md/ (P=0.005 +/- 76). BBI fell from 215 to 184 (mg/dl) for dl), elevated Anion Gap (AG>12 mEq/L), and were a 15% reduction (P=0.05 NS +/- 117). GM SQ patients treated with Glucommander. Patients were divided into had average of a 2- fold glucose reduction from baseline 2 groups: 116 patients were Appropriately Discontinued compared to patients treated with BBI (p=0.01). Day of (AD) from GM once their AG resolved (</=12 mEq/L) Discharge glucose average for GM SQ was 163 directly at and 70 patients were Prematurely Discontinued (PD) the midpoint of the target range of 140-180 (mg/dl). from GM with an AG (>12). GlucoSurveillaceTM was Discussion: Use of the eGlycemic Management System GM used to discover patients with controlled glucose (<180 SQ to treat hyperglycemic patients on SQ insulin showed mg/dl) and resolved AG (<12 mEq/L). There was no dramatic reductions of up to 56% in hypoglycemia in the first significant difference in the two study groups with respect 30 days of use and 2- fold improvements in hyperglycemia to hemogloblin A1c, age, BMI, admission glucose or AG. compared to use of BBI treatment orders. Results: There was no significant difference in glucose Conclusion: Our results support that GM SQ is a safe and at discharge between the two Glucommander groups: clinically effective tool for improving glycemic control for PD group (187 mg/dl +/- 83) and AD group (178 mg/dl patients who need SQ insulin treatment while in the hospital. +/- 69). 100% of patients in both GM groups achieved target glucose. Hypoglycemia was low in both groups, Abstract # 1306 but there was an increase in Hypoglycemia when GM was discontinued, with GM patients (0.1%) < 40 mg/ GLYTEC’S E-GLYCEMIC MANAGEMENT dl compared with (0.9%) < 40 mg/dl for Post GM care. SYSTEM IN COMBINATION WITH REAL- Length of stay was reduced by 2.4 days with AD patients TIME CLINICAL SURVEILLANCE REDUCES at 5.33 LOS compared to PD patients at 7.7 LOS. HYPOGLYCEMIA AND IMPROVES LENGTH Discussion: This study shows the clinical impact OF STAY IN HOSPITALIZED PATIENTS WITH of using the tools available in the eGlycemic DIABETIC KETOACIDOSIS Management System™, such as Glucommander™ and GlucoSurveillance™, to improve the efficacy, safety and Joseph Aloi1, Anthony Williams2, Robby Booth3, Harry efficiency of care provided to DKA patients in the hospital. Hebblewhite3, Raymie McFarland3, Bruce Bode4 Conclusion: Glucommander™ improved patient safety by reducing the incidence of hypoglycemia in patients 1. Eastern Virginia Medical School, Norfolk, VA, United with DKA, while bringing 100% of these patients into States. 2. Jackson-Madison County General Hospital, the glycemic target. Length of Stay was reduced by over Jackson, TN, United States. 3. Glytec LLC, Greenville, 2 days for patients treated with Glucommander™ and SC, United States. 4. Piedmont Hospital, Atlanta, GA, appropriately discontinued. United States. Diabetes/Prediabetes Objective: Ongoing efforts for improving quality in the care of persons with diabetes frequently focus on avoiding unnecessary hospitalizations, decreasing length of stay and avoiding readmission to hospital following discharge. Additionally, as reimbursement shifts towards - 210 - ABSTRACTS – Late Breaking Abstract # 1307 Discussion: While steroid-induced hyperglycemia will not be effectively treated in all patients with DPP4-i EFFECT OF SITAGLIPTIN ON SHORT-TERM therapy, for those with mild dysglycemia, these well METABOLIC DYSREGULATION OF ORAL tolerated oral agents represent an attractive therapeutic GLUCOCORTICOID THERAPY option. Ease of dosing and safety profiles suggest they can be used without significant risk, and may ameliorate Madiha M. Alvi, Daryl J. Selen, Marilynn Roth, Janice adverse metabolic effects of glucocorticoids. Bunn, Annis M. Marney Conclusion: This suggests DPP4-i therapy may enhance insulin sensitivity during MMT with glucocorticoid Division of Endocrinology and Diabetes, The University therapy, but results remain preliminary in this small cohort. of Vermont College of Medicine, South Burlington, VT, United States. Abstract # 1308 Diabetes/Prediabetes EARLY COMBINATION TREATMENT IMPROVES GLYCEMIC GOAL ATTAINMENT Objective: Steroid induced hyperglycemia, a complication IN TYPE 2 DIABETIC PATIENTS FAILING of glucocorticoid therapy, occurs through mechanisms METFORMIN MONOTHERAPY: A META- including impaired insulin secretion and insulin resistance. ANALYSIS DPP-4 inhibitors (DPP4-i) represent attractive therapeutic agents to mitigate dysglycemia with glucocorticoids due Olivia Phung1, Bik-Wai Tai1, Ernest Abbey1, to ease of dosing and side effect profile. Jacob Arslanian1, Swapnil N. Rajpathak2 Methods: We are conducting a prospective, randomized, double-blind, placebo-controlled, crossover study 1. College of Pharmacy, Western University of Health to evaluate effects of DPP4-i therapy on metabolic Sciences, Pomona, CA, United States. 2. Merck & Co, dysregulation of oral glucocorticoid therapy in subjects Inc., Whitehouse Station, NJ, United States. with pre-diabetes. 6 subjects have completed the study and we present here preliminary results. Dosages and Diabetes/Prediabetes durations of therapy are 2.5 mg Dexamethasone (dex) daily plus either placebo or sitagliptin (sita) 100 mg daily Objective: Type 2 diabetic patients on metformin often for 7 days. Subjects were randomized to order of study need treatment intensification with an add-on drug to reach drug: dex + placebo (pla) versus dex + sita. Each study glycemic targets. However, the benefit of appropriate period was followed by a wash out period (average 62±20 intensification in terms of glycemic goal attainment has days) before crossover. not been adequately quantified. We conducted a meta- Results: Demographics for subjects enrolled to date analysis of existing data from randomized controlled trials are as follows: 4 men, 2 women; mean age 52±6 yrs; (RCTs) evaluating the impact of non-insulin antidiabetic A1c 5.9±0.2%; BMI 30.9±2.9; fasting plasma glucose drugs added to metformin on glycemic goal attainment 96.3±10.1 mg/dL with pre-diabetes determined by A1c or among patients uncontrolled on metformin monotherapy. 2 hr glucose after 75 g OGTT 140-199 mg/dL. There were Methods: Systematic literature search was first conducted no differences in baseline glucoses or insulin for IVGTT using in PubMed, Embase, and Cochrane Central through or MMT, suggesting adequate washout between study 02/2013. Random effects meta-analysis yielded relative periods. We compared insulin area under the curve for the risk (RR) and 95% confidence interval (CI) for attainment first 15 minutes (AUC0-15 mins) from IVGTT and found of A1c goal <7%. Subgroup analysis was conducted based no difference between placebo and sita on dex (AUC0-15 on duration of follow-up. mins insulin on placebo 1004±790 vs sita 967±732 µU/ml, Results: Overall, 24 RCTs (n=9,485, median follow-up p=0.87). We compared AUC0-30, AUC0-60 and AUC0-90 24 weeks; range 12-104 weeks) were included comparing for glucose on dex during MMTs and found no difference any add-on drug to metformin vs. continuing metformin between placebo and sita. While insulin values were not monotherapy. Various classes of antidiabetic drugs statistically different on study drug between placebo and included SU (1 RCT), glinide (1 RCT), TZD (3 RCTs), sita, there was a trend towards lower insulin levels on AGI (1 RCT), DPP4 inhibitor (14 RCTs), GLP1 agonist sita compared to placebo in the first 30 minutes of MMT (3 RCTs), and SGLT2 inhibitor (1 RCT). Goal attainment (AUC0-30 placebo 1748±630, sita 1338±932, p=0.10). was defined as A1c <7%. Upon meta-analysis, treatment This suggests DPP4-i therapy may enhance insulin intensification was associated with significantly increased sensitivity during MMT with glucocorticoid therapy, but likelihood of glycemic goal attainment (41% vs 25%, RR results remain preliminary in this small cohort. 2.14, 95%CI 1.81-2.54) vs. metformin monotherapy. - 211 - ABSTRACTS – Late Breaking Discussion: Benefit of adding add-on drug to metformin were improved. In March 2013, the patient ‘s autonomic was evident in both short term and medium term durations, functions worsened (E/I, Valsalva, and 30:15 ratios 1.1, as seen in subgroup analysis, with 12-18 week follow-up 1.07, 1.12 respectively) but feet and hand ESC started (7 RCTs, 44% vs 27%, RR 2.51, 95%CI 1.65-3.83) and to improve (35μS and 52μS respectively). Azathioprine 24-36 week follow-up (15 RCTs, 42% vs 20%, RR 2.02, was started. Eight days after a second cycle of IVIG in 95%CI 1.67-2.45) providing significant findings. January 2014, the patient reported for the first time less Conclusion: The use of an add-on agent to inadequately burning, shooting pains and tingling. E/I, Valsalva, and performing metformin monotherapy is associated with 30:15 ratios remained low (1.03, 1.07, and not analyzable, significant improvement in glycemic goal attainment. respectively), while foot and hand ESC scores continued to improve (43μS and 55μS respectively). NCS never Abstract # 1309 showed significant measurable change. Discussion: Goals of CIDP treatment are to reduce MEASURING SMALL FIBER FUNCTION AS A symptoms, improve functional status, and obtain long- MEANS TO ASSESS CLINICAL RESPONSE IN term remission. We found that NCS and autonomic THE TREATMENT OF CIDP function tests did not correlate well with clinical status while numerical Sudoscan scores matched closely Aaron Vinik1, Marie-Laure Nevoret2 symptomatic changes. ESC have been found to correlate well with peripheral small fiber function and neuropathic 1. Eastern Virginia Medical School, Norfolk, VA, United symptoms in DPN. States. 2. Impeto Medical, Inc, San Diego, CA, United Conclusion: The findings in this patient warrant further States. investigation of the use of Sudoscan to monitor CIDP response to therapy. Diabetes/Prediabetes Abstract # 1310 Objective: Chronic inflammatory demyelinating polyneuropathy (CIDP) is 11X more common among METFORMIN AS ADJUNCT THERAPY TO people with diabetes than the general population and is INSULIN TREATMENT IN PATIENTS WITH treatable with appropriate immunotherapy. There is no TYPE 1 DIABETES DOES NOT SIGNIFICANTLY agreement on criteria to evaluate treatment response and IMPROVE GLYCEMIC OUTCOMES: no objective measure of sensory or autonomic small fiber SYSTEMATIC REVIEW AND META-ANALYSIS function. We present the case of a patient with type 2 diabetes (T2D) and CIDP whose treatment response was Raynold Yin, Tanya Pham, Olivia Phung measurable with the Sudoscan sudomotor function test. This test may represent a new objective evaluation of the College of Pharmacy, Western University of Health treatment of CIDP. Sciences, Montclair, CA, United States. Case Presentation: The patient is a 60 year-old male initially referred to our center in August 2012 with a Diabetes/Prediabetes diagnosis of CIDP based on AAN electrodiagnostic criteria (NCS). He complained of burning, numbness, Objective: This study was intended to evaluate whether shooting pains, and gait impairment. Autonomic functions metformin therapy can show benefits in patients with were significant for low heart rate variability response T1DM by improving A1C, fasting plasma glucose (FPG) to expiration/inspiration (E/I), Valsalva maneuver and and reducing daily insulin requirements. the ratio of the RR interval for the 30th to the 15th Methods: A systematic literature search of PubMed, beat upon standing (1.08, 1.12, 1.05 respectively), and EMBASE and the Cochrane Library (through 09/2013) frequency analysis of the total spectral power, the standard sought randomized controlled trials (RCTs) evaluating deviation of the normal RR intervals (sdNN) and their metformin compared to control in patients with T1DM root mean squared (rmsSD). Sudoscan electrochemical reporting A1C, FPG or daily insulin requirements. Two skin conductances (ESC), measuring small nerve fiber investigators independently determined study selection function on the palms and soles, were very low: 23μS and data extraction with discrepancies resolved by in the feet and 32μS in the hands. After one cycle of discussion. The weighted mean differences (WMD) of intravenous immunoglobulin (IVIG – 6 doses total, 75g change from baseline (with 95% CIs) were calculated each) the patient had no change in symptoms or Sudoscan using a random-effects model while I2 statistic was used scores for feet or hands (23μS and 32μS.) However, E/I, to determine heterogeneity. Valsalva, and 30:15 ratios (1.19, 1.36, 1.39 respectively) Results: A total of 10 studies consisting of RCTs (n = 450 - 212 - ABSTRACTS – Late Breaking pts, age range 16-48 yrs, follow-up range 3-24 wks) with a HbA1c of 9.1% and was referred back to our clinic met inclusion criteria and were meta-analyzed. The one year later. Patient was started on insulin therapy on combined results showed that metformin as adjunct to her first visit and within the first month she was able insulin therapy did not significantly lower A1C -0.22% to reduce the HbA1c from 9.1% to 6.9% (gestational (95% CI = -0.51 to 0.068, I2 = 42%, n = 6 study) or weeks 9 through 13). She received intensive diabetes FPG -17.63 mg/dL (95% CI = -95.7 to 60.4, I2 = 91%, education and was encouraged to make dietary changes n = 3 studies) compared to insulin therapy alone. There and exercise regularly. Patient was seen weekly for was however, a statistically significant reduction in HbA1c monitoring, blood sugars and diet log review total daily insulin requirements measured by either and insulin dose adjustments. -0.10 units/kg/day (95% CI = -0.17 to -0.029, I2 = 0%, Discussion: It is well documented that insulin requirements n = 3 studies) or -4.79 units/day (95% CI = -9.32 to increase gradually during the pregnancy. The “amount” -0.25, I2 = 0%, n = 3 studies). of insulin resistance can be best observed by nighttime Discussion: Although it has been suggested that insulin requirements that are needed to achieve morning metformin promotes glucose control in patients with euglycemia. The nighttime is the only time that is not T1DM, there is insufficient evidence in the literature affected by daily activities such as diet, lifestyle, exercise. to make a definitive conclusion about the effects of Our patient was able to maintain euglycemia during the metformin in patients with T1DM. day without long-acting insulin, however at bedtime she Conclusion: Results suggest that metformin as an adjunct did require 50 units of NPH insulin by the 36th week agent in patients with T1DM may help to reduce the daily (almost 50% greater than pregnant women with T1DM. insulin requirements in patients with T1DM compared This large dose of NPH is the true representation of the to placebo control. There was no statistically significant severity of insulin resistance at the end of her pregnancy. effect on either A1C or FPG with metformin as an adjunct Her prandial blood sugars were well controlled with 20- to insulin therapy in patients with T1DM. 30 units of rapid-acting insulin by the 36th week. During the third trimester, when insulin resistance reaches it’s Abstract # 1311 peak, our patient achieved a HbA1c of 5.5 - 6.1%. Patient delivered early at 36 weeks a healthy 6lb 12oz (3060g) CASE REPORT AND CLINICAL ANALYSIS: TYPE baby with APGAR 8 and 9 on minutes 1 and 5 accordingly. 2 DIABETES BEFORE, DURING AND AFTER THE Her insulin requirement decreased from 130 units TDI at PREGNANCY the end of the pregnancy to 60 units postpartum. During breastfeeding, bedtime NPH requirement decreased from Kateryna Markova1, Lois Jovanovic2 40 to 10 units nightly. Conclusion: It is important to individualize insulin 1. Santa Barbara Cottage Hospital, Santa Barbara, CA, therapy in pregnancy with the notion of evolving insulin United States. 2. Sansum Diabetes Research Institute, resistance to prevent dreaded complications to the mother Santa Barbara, CA, United States. and child. Diabetes/Prediabetes Objective: Obstetricians more often then endocrinologists see the consequences of uncontrolled diabetes in women - infertility, miscarriage, preeclampsia, birth defects, birth injuries to mother and child are well known complications of diabetes in pregnancy. Gestational diabetes alone complicates 18% of pregnancies in the US and 10.8% of all women aged 20 or older have diabetes. This case report describes what has now become a frequent scenario and presents an opportunity to discuss pathophysiology and therapeutic approach to T2DM in pregnancy. Case Presentation: Our patient is a 30 year old Hispanic female with well controlled hypothyroidism prior and during pregnancy and uncontrolled T2DM diagnosed 3 years earlier. Patient’s initial visit to clinic was after a missed abortion a year prior, her HbA1c at the time was 10.7%. Lost to follow up, she was able to conceive again - 213 - ABSTRACTS – Late Breaking Abstract # 1312 associated complication) have not been studied. Diabetes, characterized by endothelial dysfunctions and defective SHORT TERM CONTINUOUS SUBCUTANEOUS insulin mediated vasodilation may explain ED. CSII INSULIN INFUSION THERAPY (CSII) by improving endothelial dysfunctions may explain SIGNIFICANTLY IMPROVES ERECTILE improvement of vasodilatory functions of Carpora DYSFUNCTIONS IN PATIENTS WITH TYPE-2 Cavernosa. Our study,first time observed that CSII is DIABETES MELLITUS clinically significant & effective for improvement of ED. Conclusion: 1)Short Term CSII therapy significantly Kiran Singh1, Preet E. Rai1, Rakesh Talwar2, improved ED as achievement of Grade 4 erections was Sailesh Lodha3 four times higher in this group (4/23 patients) compared to MDI (1/23) as well as the overall response rate (Grade 1. Fortis Hospital, Chandigarh, Chandigarh, India. 2. 3 and 4 erections) was 2.25 times higher in CSII group.2) Haryana Raj Bhavan Dispensary, Chandigarh, UT Both the groups showed significant reduction in HbA1c, Chandigarh, India. 3. Fortis Hospital, Jaipur, Rajasthan, more marked in CSII.3)We recommend a large scale study India. for CSII usage in ED. Diabetes/Prediabetes Abstract # 1313 Objective: To determine the efficacy of 12 weeks INSIGHT INTO THE MOLECULAR MECHANISM CSII Vs basal bolus MDI among uncontrolled T2DM OF ACTION OF BTI320, A NON -SYSTEMIC patients with ED. NOVEL DRUG TO CONTROL SERUM GLUCOSE Methods: This is a comparative study over a period LEVELS IN INDIVIDUALS WITH DIABETES. of 12 weeks from two endocrine centers on 46 T2DM patients (age 32 to 60 y, mean age 43.8 y) who presented Laura Trask2, Michelle C. Miller1, Yael Bobruff2, with uncontrolled hyperglycemia (FPG > 200 mg/dl, Peter Sheehan2, David Platt2, Christopher Parkin2, HbA1C>10.0 %) & having ED and were on multiple oral Kevin H. Mayo1 hypoglycemics. All of these patients had ED as graded on the Erectile Hardness Grading Scale 1 to 4 (EHGS) 1. University of Minnesota, Minneapolis, MN, United [European Association of Urology]. EHGS grade 4 States. 2. Boston Theraoeutics Inc, Manchester, NH, indicates completely hard and fully rigid erections, Grade United States. 3 enough for penetration only, grade 2 means the penis is hard, but not hard enough for penetration and Grade 1 Diabetes/Prediabetes indicates that the penis is larger than normal, but not hard. All the study patients had EHGS grading of 1 to 2 only. Objective: Starch is an α(1-4)-linked polymer of The patients were randomized into 2 treatment groups, 23 glucose, which is enzymatically digested or hydrolyzed patients were put on MDI while on another 23 CSII was down to smaller polysaccharides (e.g. dextrin) and then initiated to manage severe hyperglycemia. Patients in both onto smaller sugars like maltotriose and maltose, and the groups were matched in terms of duration of diabetes eventually to glucose. The key enzymes responsible & complications, BMI, HbA1c, creatinine clearance, S for the breakdown of starch are generally called testosterone, FT4 TSH & prolactin levels. Stamp test & α-glycosidases, with α-amylase being primary among International Index of Erectile Function (IIEF-5) were them. BTI320 is a novel, non-systemic therapy that safely recorded. EHGS scale was recorded by patients in their reduces postprandial glucose excursions with reduced diary at -7 day, baseline & weekly till the end of study. side effects compared with acarbose. Acarbose is a natural Lispro was used in CSII and for bolus in MDI group microbial pseudo-tetrasaccharide that binds reversibly along with glargine. All the study patients gave informed and competitively to the oligosaccharide binding site of consent for not using drug or device for ED. α-glucosidases. BTI320 is composed of non-glucose- Results: Baseline HbA1c in CSII group was 11.2% and containing polysaccharides. It is essentially a composite 8.7% at the end of study while in MDI group it was 11.1% of two modified galactomannans: GM-α (1-1) linked and 9.1% respectively. In CSII group, 4 patients achieved polymer; and GM-β (1-4) linked polymer. We believe Grade 4 erections, 5 achieved Grade 3 erections (response that BTI320 functions by targeting several polysaccharide rate 39.1%) while in MDI,one achieved Grade 4 & three hydrolyzing enzymes and that the active ingredient is recorded Grade 3 response (17.3%). GM-α. The present study was focused on assessing the Discussion: Usage of CSII is increasing in T2D for molecular mechanism of action of BTI320 in relationship better control, though its effects on ED (a commonly to α-amylase. - 214 - ABSTRACTS – Late Breaking Methods: We used 1H and 13C nuclear magnetic resonance in patients with T2DM enrolled in a placebo (PBO)- (NMR) spectroscopy to investigate interactions between controlled study of CANA monotherapy. BTI320 components, GM-α and GM-β, and the enzyme Methods: In this 26-week, randomized, double-blind, α-amylase, as well as the effects that GM-α and GM-β PBO-controlled, Phase 3 study, patients with T2DM have on the rates of amylase-mediated starch hydrolysis inadequately controlled with diet and exercise (N = 584; towards glucose. The amylose iodine assay was also used mean age, 55.4 y; A1C, 8.0%; body weight, 86.8 kg) to assess starch hydrolysis. Results are compared with received CANA 100 or 300 mg or PBO once daily. The those on acarbose. distribution of change from baseline in A1C and body Results: Chemical shift changes in NMR spectra of weight at Week 26 in individual patients was analyzed as α-amylase demonstrate that GM-α interacts with the a composite endpoint. enzyme, possibly at or near its active site; GM-β appears Results: At Week 26, significant reductions in A1C to have no effect on α-amylase. GM-α and GM-β both (–0.77%, –1.03%, and 0.14%) and body weight (–2.5 kg interact with starch and apparently change the amylose [–2.8%], –3.4 kg [–3.9%], and –0.5 kg [–0.6%]) were structure, thus affecting how amylase hydrolyses the observed with CANA 100 and 300 mg compared with starch. Under certain conditions, the rate constants PBO, respectively. A greater proportion of patients treated for starch (1 mg/ml) hydrolysis with α-amylase goes with CANA 100 and 300 mg compared with PBO had from 22.5 s-1 in the absence of GM-α to 2.7 s-1 in the reductions in both A1C and body weight (70.7%, 84.0%, presence of 4 mg/ml GM-α (p<0.005). The effect on and 27.5%, respectively); PBO-subtracted differences the rate of starch hydrolysis with acarbose is similar, (95% confidence interval) were 43.2% (33.6, 52.8) but at comparatively lower acarbose concentrations. In and 56.5% (47.8, 65.2) with CANA 100 and 300 mg, addition, comparison of NMR data on α-amylase with respectively. CANA was generally well tolerated and was GM-α and acarbose suggest that GM-α binds at or near associated with an increased incidence of genital mycotic the same site on the enzyme as acarbose. infections, urinary tract infections, and osmotic diuresis- Discussion: GM-α acts as an inhibitor, possibly related adverse events compared with PBO and a low competitive, of α-amylase function, and appears to be the incidence of hypoglycemia. active ingredient in BTI320. Conclusion: The majority of patients with T2DM treated Conclusion: Our findings provide insight into how with CANA monotherapy achieved reductions in both BTI320 may function in vivo and support the potential A1C and body weight at 26 weeks. viability of BTI320 as an alternative to acarbose therapy for glycemic control. Abstract # 1315 Abstract # 1314 IMPROVED INSULIN SENSITIVITY IN PRADER- WILLI SYNDROME ON CANAGLIFLOZIN CANAGLIFLOZIN MONOTHERAPY PROVIDES REDUCTIONS IN BOTH A1C AND BODY Amy Lee, Moti Kashyap WEIGHT IN PATIENTS WITH TYPE 2 DIABETES MELLITUS University California Irvine, Irvine, CA, United States. William Canovatchel1, Michael Davies2, Diabetes/Prediabetes Ujjwala Vijapurkar1, Gary Meininger1 Objective: Is to report on a female patient with Pader- 1. Janssen Research & Development, LLC, Raritan, Willi syndrome (PWS) and her response to Canagliflozin NJ, United States. 2. Janssen Scientific Affairs, LLC, (CAGZ) for insulin sensitivity, food intake, and glycemic Raritan, NJ, United States. control. PWS is associated with obesity and diabetes with insulin resisitance.Bariatric surgery and topiramate have Diabetes/Prediabetes been unsuccessful. Metformin improves the sensation of satiety in some (30%) of PWS patients. GLP-1 receptor Objective: Canagliflozin (CANA) is a sodium glucose co- agonists have been effective. CAGZ is a sodium glucose transporter 2 inhibitor approved for the treatment of adults co-transporter inhibitor that lowers renal glucose threshild with type 2 diabetes mellitus (T2DM). CANA treatment thereby increasing glucose excretion. Hyperglycemia has been associated with significant reductions in A1C causes increased insulin resistance and removal of glucose and body weight across Phase 3 studies in a broad range toxicity after CAGZ treatment would hypothetically of patients with T2DM. This analysis explored the impact improve insuin sensitivity. of canagliflozin on A1C and body weight reduction Methods: Thirty-two-year old female with PWS (BMI - 215 - ABSTRACTS – Late Breaking of 50 kg/m2 and HbA1c 11.2%, daily Lantus insulin and Abstract # 1316 exenatide XR weekly) underwent 2-h OGTT before and at 2 wks and 8 wks after taking 300mg of CAGZ daily. THE EFFECT OF TUMOR NECROSIS FACTOR- C-Peptide and glucose levels were measured at time 0, 1h ΑLPHA ANTAGONISTS ON FASTING BLOOD and 2 h after 75 g of glucose. In addition, she underwent a GLUCOSE LEVELS AND HEMOGLOBIN A1C IN cereal breakfast meal test. The study counted the number PATIENTS WITH RHEUMATOID ARTHRITIS of boxes of Raisin Brain cereal (120 kcal/box mixed with 4 oz of 2% milk). She consumed in 3 consecutive 10- Radu Butuc1, Cosmin Dascalu2, Gabriel Pokhai1, min eating periods with a 5-min resting interval between Sakine Sever1, Adriana Abrudescu3, Sabiha Bandagi3, eating periods. Issac Sachmechi4 Results: C-PEPTIDE (pM) and GLUCOSE (mM) data during 2-h OGTT (at time 0,1h and 2h) before and at 2 1. Department of Medicine / Queens Hospital Center, and 8 wk after CAGZ. Icahn School of Medicine at Mount Sinai, Flushing, NY, At time 0 on OGTT, glucose declined from 11.7--- 9.7(2 United States. 2. Rheumatology Division/ The North wk) --- 7.8 (8 wk). At time 1h on OGTT, glucose fell from Shore-Long Island Jewish Medical Center, New Hyde 20---17.8 (2 wk) ---14.8 (8 wk). At time 2h on OGTT, Park, NY, United States. 3. Department of Medicine, glucose levels were reduced from 16.7---16.1(2 wk)--- Rheumatology Division/ Queens Hospital Center, Icahn 11.7(8 wk). School of Medicine at Mount Sinai, Flushing, NY, C-PEPTIDE levels at time 0 were 3.0---3.1 (2 wk) ---2.4 United States. 4. Department of Medicine, Division of (8 wk). C-PEPTIDE levels at 1h were 4.6---4.9 (2 wk)- Endocrinology / Queens Hospital Center, Icahn School of --4.2(8 wk). C-PEPTIDE levels at 2 h were 5.3---5.8 (2 Medicine at Mount Sinai, Flushing, NY, United States. wk)---4.5 (8 wk). CALCULATED C-PEPTIDE areas under the curve (pMx min) were 525 before CAGZ and Diabetes/Prediabetes 538 (2 wk) and 453 (8 wk) after CAGZ. Fasting insulin fell from 39--18.9 (2 wk)---12.8 (8 wk). Objective: This study aims to evaluate the effect of TNF-α Discussion: The data showed (1) Eight wks of CAGZ antagonists on fasting blood glucose (FBG) levels and resulted in a fall in fasting glucose from 11.7 to 7.8 hemoglobin A1c (HbA1c) in patients with Rheumatoid mM with concurrent lowering in HbA1C from 11.2 to Arthritis (RA). 8.9% (2) Improved glycemic control without a change Methods: FBG, HbA1c values and type of RA therapy were or increase in stimulated c-peptide and c-peptide area reviewed in 284 patients with RA. Patients were divided under curve(AUC) indicated increaed insulin sensitivity in four main groups depending on type of therapy (TNF-α after CAGZ (3) Before CAGZ she ate the same amount inhibitors versus traditional DMARDs) and diabetes (380 kcal) in each of 3 eating periods(1140kcal) without status. FBG and HbA1c values were compared before a decline in the 2nd and 3rd intervals implicating loss and after treatment with TNF-α antagonists in diabetic of normal sensory specific satiety in PWS and (4) after and non-diabetic patients respectively. Additionally CAGZ, no was no decrease in food intake detected. same values were compared between patients on TNF-α Conclusion: CAGZ restored insuin sensitiviy in PWS that antagonists therapy and patients on DMARD therapy only was mediated by removal of glucose toxicity. (control group). Incidence of diabetes was reviewed. Results: No statistically significant difference was seen when comparing FBG and HbA1c values before and after anti-TNF-α therapy in non-diabetic and diabetic patients respectively. Likewise no difference was seen when comparing the same values between patients on TNF-α antagonists with control group (on traditional DMARDs). The difference in diabetes incidence between patients receiving DMARDs and patients receiving TNF-α antagonists was not statistically significant. Discussion: TNF-α is an important mediator of insulin resistance in obesity and diabetes through its ability to decrease insulin responsiveness at the cellular level. The effect of TNF-α inhibitors on glucose homeostasis remains controversial. Few studies suggested a possible improvement in insulin resistance with TNF-α antagonists use in patients with RA. Few case reports describe - 216 -