Journal of Immunological Methods, 154 (1992) 275-276 © 1992 Elsevier Science Publishers B.V. All rights reserved 0022-1759 /92/$05.00 Contents to volume 154 (1992) Number 1 — 18 September 1992 Research reports The use of nylon wool for the isolation of T lymphocyte subpopulations D. Kokkinopoulos, S. Perez, R. Sotiriadou, J. Stinios and M. Papamichail (Greece) Lymphocyte immunophenotyping by flow cytometry in normal adults. Comparison of fresh whole blood lysis technique, Ficoll-Paque separation and cryopreservation M.A. Romeu, M. Mestre, L. Gonzdlez, A. Valls, J. Verdaguer, M. Corominas, J. Bas, E. Massip and E. Buendia (Spain) The detection of intracytoplasmic interleukin-la, interleukin-18 and tumour necrosis factor a expression in human monocytes using two colour immunofluorescence flow cytometry M.P. De Caestecker, B.A. Telfer, I.V. Hutchinson and F.W. Ballardie (UK) Selective generation of antigen-specific human hybridomas optimized for large scale growth in serum-free medium A.B. Lang, U. Schiirch, F. Zimmermann and U. Bruderer (Switzerland, Germany) Monoclonal antibody based ELISAs for cryptococcal polysaccharide A. Casadevall, J. Mukherjee and M.D. Scharff (USA) Analysis of high complement levels in Mus hortulanus and BUB mice G.L. Ong, A.E.M. Baker and M.J. Mattes (USA) Differential membrane labelling of human lymphocyte subsets by PKH-2 examined by multiparameter flow cytometry. A possible correlation between lipid composition of cellular membranes and functional properties? R. Festin (Sweden) Determination of the immunoreactive fraction of radiolabeled monoclonal antibodies directed against intracellular antigens H.J. Haisma, H.M. Pinedo, C.A. Silva and E. Boven (Netherlands) Increased specificity and sensitivity of insulin antibody measurements in autoimmune thyroid disease and type I diabetes M.J. Hegewald, S.L. Schoenfeld, D.K. McCulloch, C.J. Greenbaum, L.J. Klaff and J.P. Palmer (USA) Use of defined oligosaccharide epitopes in an ELISA for group B streptococcus R.J. Chalifour, F. Michon, H.J. Jennings and M. Lacroix (Canada) Microplate washing: process description and improvements T. Beumer, E. Stoffelen, J. Smits and W. Carpay (Netherlands) The development of enzyme-linked immunosorbent assays (ELISA) for the catecholamines adrenalin and noradrenalin J.F. Murphy, D.H. Davies and C.J. Smith (UK) A simple fluorescence method for surface antigen phenotyping of lymphocytes undergoing DNA fragmentation J.A. Hardin, D.H. Sherr, M.A. DeMaria and P.A. Lopez (USA) Effective blocking of natural cytotoxicity of young rabbit serum on murine thymocytes by high concentration of glucose in complement-dependent cytotoxicity method S. Yong Park, M. Kojima, H. Suzuki and N. Shinohara (Japan) A rapid and sensitive cellular enzyme-linked immunoabsorbent assay (CELISA) for the detection and quantitation of antibodies against cell surface determinants. I. A comparison of cell fixation and storage techniques K.W. Walker, R. Llull, G.K. Balkian, H.S. Ko, K.M. Flores, R. Ramsamooj, K.S. Black, C.W. Hewitt and D.C. Martin (USA) A transient transfection system for identifying biosynthesized proteins processed and presented to class I MHC restricted T lymphocytes L.C. Eisenlohr, J.W. Yewdell and J.R. Bennink (USA) Letter to the editors Buffering requirements for cAMP determination by radioimmunoassay in cultured macrophages C.R. Roland, K.J. Martin and M.W. Flye (USA) Book review Announcement from the publisher 276 Notes to authors Number 2 — 2 October 1992 Research reports A quantitative objective method for the evaluation of anti-sperm cell-mediated immunity in humans D.G. Dimitrov, R. Sedlak, K. Nouza and R. Kinsky (Czechoslovakia) Consistency of routine measurements of CD4*, CD8* peripheral blood lymphocytes J.P. Aboulker, B. Autran, K. Beldjord, F. Touraine and P. Debre (France) Analysis of human IgA subclasses by in situ hybridization and combined in situ hybridization / immunohistochemistry K.B. Islam, B. Christensson, L. Hammarstrém and C.1.E. Smith (Sweden) Enumeration of interleukin-1 alpha and beta producing cells by flow cytometry LC. Chikanza, V. Corrigal, G. Kingsley and G.S. Panayi (UK) Antibody to recombinant murine tumor necrosis factor (TNF) neutralizes guinea pig TNF R. Ruppel-Kerr, J.A. Lemp and M.H. Karol (USA) A new and simple method for studying the binding and ingestion steps in the phagocytosis of yeasts J. Giaimis, Y. Lombard, M. Makaya-Kumba, P. Fonteneau and P. Poindron (France) Effect of fixation on the detection of transmissible gastroenteritis coronavirus antigens by the fixed-cell immunoperoxidase technique L.-T. T6 and S. Bernard (Viet Nam, France) Rapid DNA typing utilizing immobilized oligonucleotide probe and a nonradioactive detection system. Application to HLA-DR typing of the Japanese population A. Abe, M. Kitagawa, Y. Ikuta, S. Miyakoshi, H. Danbara, N. Kashiwagi and F. Obata (Japan) Isolation of mouse Lyt2~™ mutant cells using magnetic particles D. Iannelli, R. Capparelli, F. Scala and L. Ferrara (Italy) A quantitative method for measuring the adherence of group B streptococci to murine peritoneal exudate macrophages A.R. Sloan and T.G. Pistole (USA) Factors influencing HIV-1 banding patterns in miniaturized Western blot testing of dried blood spot specimens T.C. Granade, S§.K. Phillips, C.J. Bell, C.-P. Pau, B. Parekh, W.H. Hannon, M. Gwinn, M.A. Redus, G. Schochetman and J.R. George (USA) In vitro proliferation and the cytotoxic specificity of a cryopreserved cytotoxic T cell clone reacting against human autologous tumor cells Y. Wada, H. Ikeda, D. Ueda, M. Ohta, S. Takahashi, K. Hirata, N. Sato and K. Kikuchi (Japan) High gradient magnetic separation of cells on the basis of expression levels of cell surface antigens Pcaepa e T.E. Thomas, S.J.R. Abraham, A.J. Otter, E.W. Blackmore and P.M. Lansdorp (Canada) Dendritic cells from mouse bone marrow: in vitro differentiation using low doses of recombinant granulocyte-macrophage colony-stimulating factor C. Scheicher, M. Mehlig, R. Zecher and K. Reske (Germany) eADmrtT eR EGE Letters to the editors cDNA clones of myeloperoxidase (MPO) and eosinophil peroxidase (EPO) from human blood mononuclear cells differenti- ated into granulocyte precursors in liquid cultures M. Romano, C. Melo, F. Baralle and P. Dri (Italy) Protein growth factor(s) from C6 glioma cells. A reaction to an article by Gomathi et al. (JIM 139 (1991) 101-105) S. Kumar Sharma (India) Reply to the letter of Sanjeev Kumar Sharma S.K. Sharma (India) Software notice Software archive. Current contents (August 18, 1992) Contents to volume 154 (1992) Notes to authors