VOL. 597, NOS. 1+2 JOURNAL OF CHROMATOGRAPHY APRIL 24, 1992 CONTENTS 9TH INTERNATIONAL SYMPOSIUM ON AFFINITY CHROMATOGRAPHY AND BIOLOGICAL RECOGNITION, YOKOHAMA, SEPTEMBER 24-28, 1991 Preface by S. Ishii Trypsin and affinity chromatography (Review) by K. Kasai (Kanagawa, Japan) Galactosylation as a tool for the stabilization and immobilization of proteins (Review) by J. Turkova, S. Vohnik, §. Helusova, M. J. Bene’ and M. Ticha (Prague, Czechoslovakia) Interactions and uses of antisense peptides in affinity technology (Review) by I. Chaiken (King of Prussia, PA, USA) Cellulose bearing covalently linked copper phthalocyanine trisulphonate as an adsorbent selective for polycyclic compounds and its use in studies of environmental mutagens and carcinogens (Review) by H. Hayatsu (Okayama, Japan) Study of high-affinity interactions by quantitative affinity chromatography. Analytical expressions in terms of total ligand concen- tration by D. J. Winzor and P. D. Munro (Brisbane, Australia) and C. M. Jackson (Detroit, MI, USA) Recent developments in quantitative affinity chromatography (Review) by D. J. Winzor (Brisbane, Australia) Computer simulation of weak affinity chromatography by M. Wikstré6m (Lund, Sweden) and S. Ohlson (Logan, UT, USA) Base-atom recognition in protein adsorption to alkyl agaroses by H. P. Jennissen and A. Demiroglou (Essen, Germany) Avid AL, a synthetic ligand affinity gel mimicking immobilized bacterial antibody receptor for purification of immunoglobulin G by T. T. Ngo and N. Khatter (Tustin, CA, USA) Immobilized lectin columns: useful tools for the fractionation and structural analysis of oligosaccharides (Review) by A. Kobata and T. Endo (Tokyo, Japan) Immobilization of protein ligands with methyl vinyl ether—maleic anhydride copolymer by K. Isosaki, N. Seno, I. Matsumoto, T. Koyama and S. Moriguchi (Tokyo, Japan) Affinity purification of proteins using expanded beds by H. A. Chase and N. M. Draeger (Cambridge, UK) Immobilized ferredoxins for affinity chromatography of ferredoxin-dependent enzymes by N. Sakihama, K. Nagai, H. Ohmori, H. Tomizawa, M. Tsujita and M. Shin (Kobe, Japan) Membrane-based receptor affinity chromatography by M. Nachman (Nutley, NJ, USA), A. R. M. Azad (Marlborough, MA, USA) and P. Bailon (Nutley, NJ, USA) Kinetic aspects of membrane-based immunoaffinity chromatography by M. Nachman (Nutley, NJ, USA) Short-cut method for predicting the productivity of affinity chromatography by S. Yamamoto and Y. Sano (Ube, Japan) Arginine-tail method, an affinity tag procedure utilizing anhydrotrypsin agarose by J. Hirabayashi and K. Kasai (Kanagawa, Japan) . Novel affinity separations based on perfluorocarbon emulsions. Use of a perfluorocarbon affinity emulsion for the purification of human serum albumin from blood plasma in a fluidised bed by G. E. McCreath, H. A. Chase, D. R. Purvis and C. R. Lowe (Cambridge, UK). . . . Vill CONTENTS Improved affinity chromatographic purification of D-mannose—N-acetyl-D-glucosamine-specific lectin from the bark of Sophora japonica eliminating the loss by sugar specific self-aggregation by M. Ueno, T. Arai, K. Kojima, H. K. Ogawa, I. Matsumoto and N. Seno (Tokyo, Japan) .......... =. #197 Selective clean-up method using an immunoaffinity column following radioimmunoassay of prostaglandin F ,, in biological fluids by Y. Murai, M. Ohno, M. Konishi and G. Kominami (Osaka, Japan) .........2.2..2.2.2.4.2.4.+.. 201 Isolation and characterization of isolectins from Erythrina variegata seeds by N. Yamasaki, M. Kimura, O. Yamaguchi and M. Araki (Fukuoka, Japan) .........2.2.2.2.2.2.. =. 207 Purification and characterization of carbohydrate-binding peptides from Lotus tetragonolobus and Ulex europeus seed lectins using affinity chromatography by Y. Konami, K. Yamamoto and T. Osawa (Tokyo, Japan) ................2. 2446442444. . 213 Determination of the carbohydrate-binding site of Bauhinia purpurea lectin by affinity chromatography oy mh. Veet, Y. Romami and 7. Oenwe (Tokyo, Japam) . . 2. te ttt he tee Se Evaluation of the mode of binding of immunoglobulin to activated agarose by W. H. Scouten, C. M. Adler, R. Rongen and L. Mallee (Waco, TX, USA) . Use of antibody fragments in immunoaffinity chromatography. Comparison of FV fragments, VH fragments and paralog pep- tides seae 2, Se C O ED ee ee ee wee Ree ee ew ee bh ev we ee Affinity chromatographic purification of immunoglobulin M antibodies utilizing immobilized mannan binding protein by J. R. Nevens, A. K. MalliaM., W . Wendt and P. K. Smith (Rockford, IL, USA)... .........2... =. 247 Affinity purification of antibodies using immobilized FB domain of protein A by B. Solomon, O. Raviv, E. Leibman and G. Fleminger (Tel-Aviv, Israel) . . . . 2... . 2... ee «257 Calcium-modulated conformational affinity chromatography. Application to the purification of calmodulin and $100 proteins by G. Fleminger, T. Neufeld, M. Star-Weinstock, M. Litvak and B. Solomon (Tel Aviv, Israel) . . ....... . 263 High-performance affinity chromatography system for the rapid, efficient assay of glycated albumin by K. Yasukawa (Kawasaki-shi, Japan), F. Abe (Tokushima-shi, Japan), N. Shida, Y. Koizumi, T. Uchida and K. Noguchi (Kawasaki-shi, Japan) and K. Shima (Tokushima-shi, Japan)... .............. 2... 271 Artificial immunoglobulin G-binding protein mimetic to staphylococcal protein A. Its production and application to affinity purification of immunoglobulin G es er a rr er SS ek} Oe ee we ee ee ae ew we ee ke ee lee REE Purification of sufficiently y-carboxylated recombinant protein C and its derivatives. Calcium-dependent affinity shift in immu- noaffinity and ion-exchange chromatography by K. Ohsawa, M. Kimura, K. Kurosawa-Ohsawa, M. Takahashi, M. Koyama, Y. Abiko, K. Hirahara, T. Matsuishi and eete el ee oe ke ek ee eae Oe RR a Interaction of amphipathic model lipopeptides with phospholipid bilayers by S. Ono, T. Kato, S. Lee, H. Aoyagi and M. Ohno (Fukuoka, Japan) ............... 4... . 293 Purification and partial characterization of phospholipase A, isoforms from human placenta by L. M. Jordan (Greensboro, NC, USA) and F. Russo-Marie (Paris, France) ...........2.2.2.+.+. =. 299 Preparation of immunoaffinity mini-columns for the analysis of platelet activating factor (PAF) in biological samples by M. Watanabe (Miyagi, Japan and Waltham, MA, USA), T. Watanabe, N. Hirasawa and S. Mue (Miyagi, Japan), S. Muramatsu, Y. Matsushita and H. Takahagi (Tokyo, Japan), P. Braquet and C. Broquet (Le Plessis Robinson, France), L. Levine (Waltham, MA, USA) and K. Ohuchi (Miyagi, Japan) ............. 2.402.486.4444. 309 Immunoenzymatic assay of dyes currently used in affinity chromatography for protein purification by P. Santambien, P. Girot, I. Hulak and E. Boschetti (Villeneuve La Garenne, France) ..........2... 315 Affinity purification and affinity characterization of carbohydrate-binding proteins in bovine kidney by K. Kojima, H. K. Ogawa, N. Seno and I. Matsumoto (Tokyo, Japan) ........2.2..2.2.4.42.2.+.. . 323 Affinity chromatographic purification of bovine lung endothelin receptor using biotinylated endothelin and avidin—agarose by H. Hagiwara, T. Nagasawa, K. M. Lodhi, M. Kozuka, T. Ito and S. Hirose (Tokyo, Japan) .......... 33i Affinity chromatography in purification of A, adenosine receptors by H. Nakata (Tokyo, Japan) CONTENTS Analysis of the interaction between «(1 —6)dextran-specific mouse hybridoma antibody and dextran B512 by affinity electropho- resis by Y. Mimura, K. Nakamura and K. Takeo (Ube, Japan) Characterization of the interaction between human plasma fibronectin and collagen by means of affinity electrophoresis by K. Nakamura, S. Kashiwagi and K. Takeo (Ube, Japan) Immobilized metal ion affinity electrophoresis. A study with several model proteins containing histidine byH . Goubran-Botros, E. Nanak andJ .A bdul Nour iiscenee France), G. en oe Germanya)nd M. A. Vijayalakshmi (Compiégne, France). se : Pai ak a ee eas or as SESS aD ee Ce Separation of monoclonal antibodies from antihapten antisera by two-dimensional affinity electrophoresis by K. Takeo, K. Nakamura and R. Suzuno (Ube, Japan) Determination of the association constant of monovalent mode protein-sugar interaction by capillary zone electrophoresis by S. Honda, A. Taga, K. Suzuki, S. Suzuki and K. Kakehi (Higashi-osaka, Japan) Process monitoring by flow-injection immunoassay. Evaluation of a sequential competitive binding assay by M. Nilsson, H. Hakanson and B. Mattiasson (Lund, Sweden) Mapping of viral epitopes with conformationally specific monoclonal antibodies using biosensor technology by M.-C. Dubs, D. Altschuh, M. H. V. Van Regenmortel (Strasbourg, France) Biospecific interaction analysis using surface plasmon resonance detection applied to kinetic, binding site and concentration analysis by L. G. Fagerstam, A. Frostell-Karlsson, R. Karlsson, B. Persson and I. Rénnberg (Uppsala, Sweden) Occurrence of an allosteric transition in the modification of papain with L-1-acetyl-2,3-dihydropyrrolo[2,3-b]indole-2-carbox- amide by A. Nagatomo, K. Sakai, T. Nose, Y. Shimohigashi and M. Ohno (Fukuoka, Japan) Specific assay for endotoxin using immobilized histidine, Limulus amoebocyte lysate and a chromogenic substrate by M. Nawata, S. Minobe, M. Hase, T. Watanabe, T. Sato and T. Tosa (Osaka, Japan) Discriminative affinity labelling of opioid receptors by enkephalin and morphiceptin analogues containing 3-nitro-2-pyridinesul- phenyl-activated thiol residues byY . Shimohigashi, K. Takada, H. Sakamoto and H. Matsumoto (Fukuoka, = T. Yasunaga and M. Kondo — Japan) and M. Ohno (Fukuoka, Japan) 6. ae ee ‘ Purification and reconstitution of y-opioid receptors in liposome by T. Fujioka, F. Inoue, S. Sumita, M. Kuriyama, T. Hanawa and T. Katagi (Nishinomiya, Japan) Use of various types of column reactors for flow-injection analysis by M. Tabata and T. Murachi (Kyoto, Japan), J. Endo (Shimane, Japan) and M. Totani (Tokyo, Japan) . . Non-isotopic receptor assay for benzodiazepines using a biotin-labeled ligand and biotin-immobilized microtiter plate by S. Tanaka, T. Takeuchi and G. A. Rechnitz (Honolulu, HI, USA) Author Index .