VOL. 548, NOS. 1+2 JOURNAL OF CHROMATOGRAPHY JULY 12, 1991 CONTENTS 10TH INTERNATIONAL SYMPOSIUM ON HIGH-PERFORMANCE LIQUID CHROMATO- GRAPHY OF PROTEINS, PEPTIDES AND POLYNUCLEOTIDES, WIESBADEN, OCTOBER 29-— 31, 1991 Foreword THEORY Investigations of liquid—liquid partition chromatography of proteins by M.-R. Kula, L. Elling and A. Walsdorf (Julich, Germany) . . . . Hydrophilic-interaction chromatography of peptides on hydrophilic and strong cation-exchange columns by B.-Y. Zhu, C. T. Mant and R. S. Hodges (Edmonton, Canada) Evaluation of kinetic models for biospecific adsorption and its implications for finite bath and column performance by M. A. McCoy and A. I. Liapis (Rolla, MO, USA) Multivalent ion-exchange model of biopolymer chromatography for mass overload conditions by P. Cysewski (Bydgoszcz, Poland), A. Jaulmes, R. Lemque, B. Sebille and C. Vidal-Madjar (Thiais, France) and G. Jilge (Mainz, Germany) “EP o Derte EL EES Cee a Application of the split-peak effect to study the adsorption kinetics of human serum albumin on a reversed-phase support by C. Vidal-Madjar, H. Place, L. Boulkanz and A. Jaulmes (Thiais, France) Solution properties of polyelectrolytes. VII. Non-ideal mechanisms in size-exclusion chromatogra- phy of synthetic polyions, peptides and proteins by E. Perez-Paya, L. Braco, C. Abad, V. Soria and A. Campos (Valencia, Spain) High-performance liquid chromatography of amino acids, peptides and proteins. CX. Principal com- ponent analysis of four sets of group retention coefficients derived from reversed-phase high- performance liquid chromatography of peptides by M. C. J. Wilce, M. I. Aguilar and M. T. W. Hearn (Clayton, Australia) . . High-performance liquid chromatography of amino acids, peptides and proteins. CXI. Retention behaviour of proteins with macroporous tentacle-type anion exchangers by M. T. W. Hearn, A. N. Hodder, F. W. Fang and M. I. Aguilar (Clayton, Australia) High-performance liquid chromatography of amino acids, peptides and proteins. CXII. Analysis of operating parameters affecting the breakthrough curves in fixed-bed chromatography of pro- teins using several mathematical models by A. Johnston, Q. M. Mao and M. T. W. Hearn (Clayton, Australia) . High-performance liquid chromatography of amino acids, peptides and proteins. CXIII. Predicting the performance of non-porous particles in affinity chromatography of proteins by Q. M. Mao, A. Johnston, I. G. Prince and M. T. W. Hearn (Clayton, Australia) SORBENTS Characterization of synthetic macroporous ion-exchange resins in low-pressure cartridges and col- umns. Evaluation of the performance of Macro-Prep 50 S resin in the purification of anti- Klenow antibodies from goat serum by L. Dunn, M. Abouelezz, L. Cummings, M. Navvab, C. Ordunez, C. J. Siebert and K. W. Talmadge (Richmond, CA, USA) Rages Vill CONTENTS Comparison of silica-based cyanopropyl and octyl reversed-phase packings for the separation of peptides and proteins byN . E. Zhou and C. T. Mant (Edmonton, Canada), J. J. Kirkland rica anaes DE, USA) and R. S. Hodges (Edmonton, Canada) ne yee a er eee ee ae ae 179 Silica versus polymer-based stationary phases for reversed-phase high-performance liquid chroma- tographic analyses of rat insulin biosynthesis. A comparison of resolution and recovery by S. Linde and B. S. Welinder (Gentofte, Denmark) 195 Ion-exchange high-performance liquid chromatography of nucleotides and polypeptides on new types of ion-exchange sorbents, based on polystyrene-coated silicas ag A.e se and V. A. Davankov (Moscow, USSR) and K. K. —— (Mainz, Germa- 207 High-performance liquid chromatography and ultrafiltration of whey proteins with inorganic porous materials coated with polyvinylimidazole derivatives by B. Chaufer, M. Rollin and B. Sébille (Thiais, France) . 215 AFFINITY CHROMATOGRAPHY Purification of Phleum pratense pollen extract by immunoaffinity chromatography and high-perform- ance ion-exchange chromatography by E. Bolzacchini, P. Di Gennaro, G. Di Gregorio, B. Rindone, P. Falagiani and G. Mistrello (Milan, Italy) and I. Sondergaard (Copenhagen, Denmark) . Sicit Pe a eS a 229 A ten-residue fragment of an antibody (mini-antibody) directed against lysozyme as ligand in immu- noaffinity chromatography by G. W. Welling, J. van Gorkum, R. A. Damhof and J. W. Drijfhout (Groningen, Nether- lands), W. Bloemhoff (Leiden, Netherlands) and S. irri -Wester Gini Nether- lands) fry : ge rea get Pe i ila aa 235 Effect of antigen size on optimal ligand density of immobilized antibodies for a high-performance liquid chromatographic support by J. W. Wheatley (Berkeley, CA, USA) . 243 Affinity of trypsin for amidine derivatives immobilized on dextran-coated silica supports by M. Ellouali, S. Khamlichi, J. Jozefonvicz and D. Muller (Villetaneuse, France) . 255 PREPARATIVE CHROMATOGRAPHY Multi-column preparative reversed-phase sample displacement chromatography of peptides by R. S. Hodges, T. W. L. Burke and C. T. Mant (Edmonton, Canada) 267 Analysis and purification of DNA restriction fragments by high-performance liquid chromatography by P. L. Mauri, P. G. Pietta and M. Pace (Milan, Italy) 281 High-performance liquid chromatographic purification of antiviral components in Neuramide by S. Giacobbe, N. Miraglia and B. Rindone (Milan, Italy), G. Folchitto (Ariccia, wide and G. Antonelli (Rome, Italy). a ee eee eee CA als oa ee ee eee 289 ANALYTICAL APPLICATIONS Glycosylation of extracellular superoxide dismutase studied by high-performance liquid chromato- graphy and mass spectrometry by M. Stromqvist (Umea, Sweden) and J. Holgersson and B. Samuelsson (Gothenburg, Sweden) eo peat alg dete 5 athe gs Of 293 Peptide maps at picomolar levels obtained by reversed-phase high-performance liquid chromatogra- phy and pre-column derivatization with phenyl isothiocyanate. Microsequencing of phenyl- thiocarbamy] peptides by F. J. Colilla (Madrid, aie S. P. Yadav and K. Brew (Miami, FL, USA) and E. Mendez (Madrid, Spain) Sk, era ae a a ee a ae Wicca ae ia eo VPA CONTENTS Fingerprinting of molecular components in individual human cerebrospinal fluid samples with a new micropurification system by F. Nyberg, S. Lyrenas and A. Danielsson (Uppsala, Sweden) ........... 3ii High-performance liquid chromatographic determination of peptides in biological fluids by automat- ed pre-column fluorescence derivatization with fluorescamine by V. K. Boppana, C. Miller-Stein, J. F. Politowski and G. R. Rhodes (King of Prussia, PA, Separation of acidic peptides by reversed-phase ion-pair chromatography. Analytical application toa series of acidic substrates of casein kinases by A. Calderan, P. Ruzza, O. Marin, M. Secchieri, G. Borin and F. Marchiori (Padova, 24. Gree ie eed! hoe >eon & oa) yg ee wee ee we Res, oe Gea Ne a Animal test or chromatography? Validated high-performance liquid chromatographic assay as an alternative to the biological assay for ornipressin by R. H. Buck, M. Cholewinski and F. Max] (Basle, Switzerland) . . ........ =. 335 Non-radioactive detection of MHC class II—peptide antigen complexes in the sub-picomole range by high-performance size-exclusion chromatography with fluorescence detection by H. Kalbacher and H. Kropshofer (Tibingen, Germany). ............ .. 343 High-performance liquid chromatographic separation of modified and native melittin following transglutaminase-mediated derivatization with a dansyl fluorescent probe by E. Perez-Paya, L. Braco and C. Abad (Valencia, Spain) and J. Dufourcq (Pessac, France) 351 Phenotyping of bovine milk proteins by reversed-phase high-performance liquid chromatography by S. Visser, C. J. Slangen and H. S. Rollema (Ede, Netherlands) ........... 361 Separation and quantitation of serum proinsulin and proinsulin intermediates in humans by S. Linde, M. E. Reader, S. G. Hartling, C. Binder and B. S. Welinder (Gentofte, Den- mark) OTHER SEPARATION METHODS Structure-stability relationship of Immobiline chemicals for isoelectric focusing as monitored by capillary zone electrophoresis by M. Chiari, C. Ettori, A. Manzocchi and P. G. Righetti (Milan, Italy) ....... . 381 Effects of temperature, carrier composition and sample load in asymmetrical flow field-flow fraction- ation by A. Litzén (Uppsala, Sweden) and K.-G. Wahlund (Lund, Sweden) Author Index . SHLASOOTESSSSSESSSESNESSESSSESESSS EES HESESESSNS FESSEDSESSO SET O TT ET OTOH Tee T Perr Tee Teee * 4 In articles with more than one author, the name of the author to whom correspondence should be addressed is indicated in the ** * article heading by a 6-pointed asterisk (*) * * * * * * ARRIGO ORI IRR RO OI OR GIGI GI aR ak kk kk