Official Publication of ALPHA, Scientists in Reproductive Medicine JOURNAL OF ASSISTED REPRODUCTION AND Volume 21, 2004 GENETICS fin Vitro Fertilization and Embryo Transfer Journalo f Assisted Reproduction and Genetics publishes original peer-reviewed papers on assisted reproductive technologies and early genetics in all areas of the human as well as animal EDITOR-IN-CHIEF experience. The utilization of genetic diagnosis in conjunction NORBER iLEICHER, Chicago, Illinois with assisted reproductive technologies is emphasized. (A detailed description of the journal's scope is included in the EDITORS—CLINICAL TRIALS Instructions to Authors.) DAVID BARAD. New York w York {ICHAI SNABES. ( JOURNAL OF ASSISTED REPRODUCTION AND GENETICS (ISSN: 1058-0468) is published monthly by ASSOCIATE EDITORS Springer, P. O. Box 322, 3300 AH Dordrecht, The Netherlands Annual subscription price $1044.00. 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LEON BURMEISTER, Io lowa (Statistical Evaluation) LEROY WALTERS, Washington, D.C. (Experimental Design) JOURNAL OF ASSISTED REPRODUCTION AND GENETICS (ISSN: 1058-0468) is published monthly by Springer, P. O. Box 322, 3300 AH Dordrecht, The Netherlands. Annual subscription price $1044.00. Air freight and mailing by Expediting, Inc., 200 Meacham Avenue, Elmont, N.Y. 11003. Periodicals postage paid at Jamaica, N.Y. 11431. Journal of Assisted Reproduction and Genetics is abstracted or indexed in Biological Abstracts, CAB Abstracts, Current Contents/Clinical Medicine, EMBASE/Excerpta Medica, Index Medi- cus/MEDLINE, Medical Documentation Service, Science Citation Index Expanded, SCOPUS, and The ISI Alerting Services. Advertising inquiries should be addressed to Springer, Advertising Dept., 233 Spring St., New York N.Y. 10013—tel:(212) 460-1572; fax:(212) 647-1898; e-mail: [email protected] © 2004 Springer Science+Business Media, Inc. No part of this work may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the Publisher, with the exception of any material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work. Journal of Assisted Reproduction and Genetics participates in the Copyright Clearance Center (CCC) Transactional Reporting Service. The appearance of a code line at the bottom of the first page of an article in this journal indicates the copyright owner’s consent that copies of the article may be made for personal or internal use. However, this consent is given on the condition that the copier pay a flat fee per copy per article (no additional per-page fees) directly to the Copyright Clearance Center, Inc., 222 Rosewood Drive, Danvers, Massachusetts 01923 (www.copyright.com), for all copying not explicitly permitted by Sections 107 or 108 of the U.S. copyright Law. The CCC is a nonprofit clearinghouse for the payment of photocopying fees by libraries and other users registered with the CCC. Therefore, this consent does not extend to other kinds of copying, such as copying for general distribution, for advertising or promotional purposes, for creating new collective works, or for resale, nor to the reprinting of figures, tables, and text excerpts. 1058-0468/04 Journal of Assisted Reproduction and Genetics CONTENTS Volume 21, Number 1 January 2004 Alpha Page Editorial N. Gleicher ‘ Vivgulating Dremylantation Geneltc Y CUGHOS C4 SSGD/ 5 Regulations for Preimplantation Genetic Diagnosis in France M. Plachot and J. Cohen Preimplantation Genetic Diagnosis (PGD) in France Y. J.R. Menezo, R. Frydman, and N. Frydman What Regulations for Preimplantation Genetic Diagnosis? D. L. Hill and M. Li Preimplantation genetic diagnosis of ¢ lisciplinary process. Personnel qualifications in interpretatior lin ART clinics Preimplantation Diagnosis (PGD)—A European Clinician’s Point of View W. Feichtinger Regulating Preimplantation Genetic Diagnosis—How to Control PGD N. Takeshita and H. Kubo Journal of Assisted Reproduction and Genetics CONTENTS Volume 21, Number 2 February 2004 27 Alpha Page 29 ~—sE ditorial N. Gleicher . Cn deolegy 31 The Difficult MESA: Findings From Tubuli Recti Sperm Aspiration U. Zenke, L. Jalalian, S. Shen, and P. J. Turek During difficult microsurgical epididymal sperm aspiration (MESA) procedures, sperm from the tubuli recti may provide an excellent alternative to testis sperm extraction —Asstsled Riypwreduclion 37 Comparison of Basal and Clomiphene Citrate Induced FSH and Inhibin B, Ovarian Volume and Antral Follicle Counts as Ovarian Reserve Tests and Predictors of Poor Ovarian Response in IVF M. Erdem, A. Erdem, R. Gursoy, and K. Biberoglu Clomiphene citrate challenge test (CCCT) and ovarian volume measurement are better then other hormonal and ultrasonographic tests in predicting the response to ovarian stimulation in IVF cycles 47 Cumulative Embryo Score, Not Endometrial Thickness, Is Best for Pregnancy Prediction in IVF C. Laasch and E. Puscheck Cumulative embryo score alone, and not combined with endometrial thickness, is a statistically significant predictor of pregnancy in IVE ( . . Sherl Communications 51 Longevity of Donor Serum J. E. Graves, H. L. Higdon III, J. E. Johnson, D. W. Blackhurst, and W. R. Boone Embryonic Chromosomal Abnormalities Obtained After Rescue Intracytoplasmic Sperm Injection of 1-Day-Old Unfertilized Oocytes T. Pehlivan, C. Rubio, A. Ruiz, J. Navarro, J. Remohi, A. Pellicer, and C. Simon Case. Liqort 59 Successful Spouse Pregnancy of Male Patients with Severe Apalstic Anemia and Chronic Myelogenous Leukemia Using Spermatozoa Banked Prior to Bone Marrow Transplantation and Using the ICSI Procedure: Case Reports S. W. Cho, S. H. Lee, M. K. Chung, H. A. Kim, H. M. Chung, Y. J. Lee, H. Kwon, T. K. Yoon, and K. Y. Cha The cover photo is from “Embryonic Chromosomal Abnormalities Obtained After Rescue Intracytoplasmic Sperm Injection of 1-Day-Old Unfertilized Oocytes” by Pehlivan et al. Journal of Assisted Reproduction and Genetics CONTENTS Volume 21, Number 3 March 2004 63 Alpha Page Cistsled . Liywrot UClCON 65 Use of Stimulated Serum Estradiol Measurements for the Prediction of Hyperresponse to Ovarian Stimulation in Vitro Fertilization (IVF) D. J. Hendriks, E. R. Klinkert, L.F- J. M.M. Bancsi, C. W. N. Looman, J. D. F Habbema, E. R. te Velde, and F. J. Broekmans Estradiol levels measured early in the course of IVF stimulation are not predictive for the occurrence of ovarian hyperresponse Mean Ovarian Diameter (MOD) as a Predictor of Poor Ovarian Response I. K. Vladimirov, D. M. Tacheva, and K. B. Kalino Mean ovarian diameter, measurien dth e largest ne of t vary, provgoiod dproegnosst ic information for poor Ovarian response In in Vv Cpinton 79 Why Do Older Women Have Poor Implantation Rates? A Possible Role of the Mitochondria A. K. Bartmann, G. S. Romdo, E. da Silveira Ramos, and R. A. Ferriani Mutations in mitochondrial DNA may be resp r poor implantation and aneuploidy in older wome ¢ . . Sherl Communication 85 Birth Weight Discordance in Spontaneous Versus Induced Twins: Impact on Perinatal Outcome J. Zadori, Z. Kozinszky, H. Orvos, M. Katona, S. G. Kadli, and A. Pal Case e Leypor 3 89 Pregnancy and Delivery After Stimulation with rFSH of a Galatosemia Patient Suffering Hypergonadotropic Hypogonadism: Case Report Y. J. R. Menezo, M. Lescaille, B. Nicollet, and E. J. Servy 91 Testicular Sperm Extraction (TESE) and Intracytoplasmic Sperm Injection (ICSI) in Hypogonadotropic Hypogonadism with Persistent Azoospermia After Hormonal Therapy M. Meseguer, N. Garrido, J. Remohi, A. Pellicer, and M. Gil-Salom Journal of Assisted Reproduction and Genetics CONTENTS Volume 21, Number 4 April 2004 95 Alpha Page Dh Gs volcyy 97 Human Cervical Mucus Can Act in Vitro as a Selective Barrier Against Spermatozoa Carrying Fragmented DNA and Chromatin Structural Abnormalities P.G. Bianchi, A. De Agostini, J. Fournier, C. Guidetti, N. Tarozzi, D. Bizzaro, and G.C. Manicardi Spermatozoa possessing high levels of DNA protamination and low levels of DNA damage are more proficient in crossing the human cervical mucus barrie! Assisted Viywreduclion 103 Monozygotic Twins and Triplets in Association with Blastocyst Transfer J.K. Jain, R. Boostanfar, C.C. Slater, M.M. Francis, and R.J. Paulson Blastocyst transfer was associated with a significantly higher rate of monozygotic twins when compared to day-3 embryo transfet Vale. Infertility 109 Parameters Affecting the Results in a Program of Artificial Insemination With Donor Sperm: A 12-Year Retrospective Review of More Than 1800 Cycles JL. Zuzuarregui, M. Meseguer, N. Garrido, C. Simon, A. Pellicer, and J.R emohi In artificial donor insemination, being under the age of 35, having a smooth ovarian stimulation and a total number of progressive motile sperm and inseminating greate! 5 million on two consecutive days improves results DNA Fragmentation, Mitochondrial Dysfunction and Chromosomal Aneuploidy in the Spermatozoa of Oligoasthenoteratozoospermic Males C.-H. Liu, H.-M. Tsao, T:-C. Cheng, H.-M. Wu, C.-C. Huang, C.-I. Chen, D.P.-C. Lin, and M.-S. Lee Spermatozoa from oligoathenoteratozoospermic patients contain higher rates ofD NA fragmentation, mitochondrial dysfunction and chromoson ineuploidy Cpinten 127. A Proposal to Change the Method for Determining the Outcome According to the SART Statistic—Pregnancy Rate per Retrieval J.H. Check 4 new method of interpreting the chance of pregnancy per oocyte retrieval is desc does not penalize in vitro fertilization centers that empnasize cryopt reservation of emb ( ( . . Sherl Comm UWNMMOUNTCH 129 Isolation and Expression Analysis of the Testis-Specific Gene, Human OPPO] T. Miyamoto, K. Sengoku, H. Hayashi, Y. Sasaki, N. Takuma, T. Yamashita, and M. Ishikawa Journal of Assisted Reproduction and Genetics CONTENTS Volume 21, Number 5 May 2004 135 Alpha Page . Cnidrocle4 GY 137 ~—-V~ itality of Oligozoospermic Semen Samples is Improved by Both Swim and Density Gradient Centrifugation Before Cryopreservation M. Counsel, R. Bellinge and P. Burton Post-thaw vitality in oligozoc swim-up and density gradient Impact of Semen Characteristics on the Success of Intrauterine Insemination Y. Zhao, N. Vlahos, D. Wyncott, C. Petrella, J. Garcia, H. Zacur and E. E. Wallach Sperm motility before density gradient se 1 mn progressior after density gradient separ ire _bsststed . Leyes Whe. techineleyy 149 Low-Level Gonosomal Mosaicism in Women Undergoing ICSI Cycles R. Voigt, A. K. Schréder, F. Hinrichs, K. Diedrich, E. Schwinger and M. Ludwig The incidence of low-level gonosomal n I ICSI and its presednoecs en ot have an influenc tcor f ICSI Reproductive Performance of Couples Discordant for Hepatitis B and C Following IVF Treatment I. R. Pirwany, S. Phillips, S. Kelly, W. Buckett and S. 1 Hepatitis B and C discordant couples hay i pregnancy rates followinIgV I reatment, compared with age and diagnos trols | comparable response to COH Feasibility of Human Telomerase Reverse Transcriptase mRNA Expression in Individual Blastomeres as an Indicator of Early Embryo Development C.-W. Wang, D.-S. Yao, S.-G. Horng, H.-C. Chiu, C.-K. Chen, C.-L. Lee, H.-Y. Huang, H.-S. Wang, Y.-K. Soong, and C. C. Pao Although the dif blastmeres is significant in statist The Effect of a Two-hour, Room Temperature Incubation of Human Spermatozoa in TEST- Yolk Buffer on the Rate of Fertilization in \ J. R. Jensen, J. H. Walker, A. A. Milki, L. Westphal, ai We report that semen incubated in T I ym temperature, before density gradient separation generates higher Mi Maillcicilins 175 Tissue Perfusion Essential for Spermatogenesis and Outc« Extraction (TESE) for Assisted Reproduction R. Herwig, K. Tosun, G.-M. Pinggera, E. Soelder, K.1 F. Frauscher, G. Bartsch, L. Wildt, and K. Illmense. Testicular perfusion predicts sperm qua Case. Vigort 181 Bilateral Ovarian Pregnancy After in Vitro Fertilization and Embryo Transfert in a Patient with Tubal Factor Infertility M. Han, J. Kim, H. Kim, G. Je and T. Hwang Journal of Assisted Reproduction and Genetics CONTENTS Volume 21, Number 6 June 2004 185 Alpha Page LA Vg ology 187 Expression of Integrin Fraction and Adhesion Molecules on Human Granulosa Cells and Its Relation with Oocyte Maturity and Follicular Steroidogenesis A. Clavero, J. A. Castilla, L. Martinez, N. Mendoza, J. Fontes, and V. Maldonado rhe expression pattern for integrin fractions and adhesion molecules on granulosa cells may be of predictive value in integrin the differentiation of the human follicle Ciststed . Viywreduction 197 Comparison of Clinical Outcome and Costs with CC + Gonadotropins and GnRHa + Gonadotropins During IVF/ICSI Cycles P. Kovacs, S. Matyas, A. Bernard, and S. G. Kaali During IVF/ICSI, stimulation with GnRHa + gonadotropins is clinically more effective and costs less than stimulation with C¢ gonadotropins Improved Outcome of Frozen-Thawed Blastocyst Transfer with Menezo’s two-step Thawing Compared to the Stepwise Thawing Protocol J. Ding, M. Pry, N. Rana, and W. P. Dmowski Menezo’s two-step thawing method greatly improved the implantation and pregnancy rates after frozen embryo transfer when blastocysts were cryopreserved with Menezo’s two-step slow-freezing technique Impact of Body Mass Index on In Vitro Fertilization Outcomes J. L. Frattarelli and C. L. Kodama An elevated BMI correlates positively with the number of follicles, days of stimulation and number of ampules of gonadotropins used in an IVF Cycle 74, nileclogy 217 Reduced Senescence and Retained Nuclear DNA Integrity in Human Spermatozoa Prepared by Density Gradient Centrifugation J. M. Morrell, O. Moffatt, D. Sakkas, G. C. Manicardi, D. Bizzaro, M. Tomlinson, H. Nilsson, and P. V. Holmes PureSperm™ density gradient preparation of human spermatozoa reduces sperm senescence and retains sperm nuclear DNA integrity, thereby potentially prolonging sperm fertilising ability in ART . « . . _ Cnimal Caperimentation 223 Coculture with a Human Granulosa Cell Line Enhanced the Development of Murine Preimplantation Embryos Via SCF/c-Kit System F. Taniguchi, T: Harada, M. Nara, I. Deura, M. Mitsunari, and N. Terakawa KGN cells may facilitate preimplantion embryo development through SCF/c-kit paracrine rf ( . . Sherl Communication 229 Spontaneous Pregnancies in a Turner Syndrome Woman with Y-Chromosome Mosaicism K. Landin-Wilhelmsen, I. Bryman, C. Hanson, and L. Hanson Journal of Assisted Reproduction and Genetics CONTENTS Volume 21, Number 7 July 2004 Alpha Page , _tnimal Caperimentation 233. Comparison of Maturation, Fertilization, Development, and Gene Expression of Mouse Oocytes Grown In Vitro and In Vivo D.-H. Kim, D.-S. Ko, H.-C. Lee, H.-J. Lee, W.-II. Park, S. S. Kim, J.-K. Park, B.-C. Yang, S.-B. Park, W.-K Chang, and H.-T. Lee The differences of maturation, fertilizatior of in vitro- and in vivo-grown mouse oocyt : Vhystolog Y 241 The Presence of Cytokines and Growth Factors in Hydrosalpingeal Fluid A. Strandell, Jane Thorburn, and A. Wallin The analyzed cytokines were abundantly pre ydrosalping fluid compawirth efolldicul ar fluid and serum, suggesting an increased exp n the tubal epithelium —Absssled Riywred UCMCH 249 Association of Inhibin B Serum Levels with Parameters of Follicular Response in a Randomized Controlled Trial Comparing GnRH Agonist Versus Antagonist Protocols for Ovarian Hyperstimulation B. Sonntag, L. Kiesel, E. Nieschlag, and H. M. Behre Early follicular phase Inhibin B serum levels ¢ te with follicular response in ART cycles applying the long GnRH agonist, but not GnRH The Practical Implications of a Raised Serum FSH and Age on the Risk of IVF Treatment Cancellation Due to a Poor Ovarian Response V. A. Akande, S. D. Keay, L. P. Hunt, R. S. Mathur, J. M. Jenkins, and D. J. Cahill Age and FSH serve as valuable indicators of IVF t nent cancellation. However, among older women, FSH has particular importance, while less s Does Seasonality Alter Intrauterine Insemination Outcomes: A 5-Year Study J. G. Proctor, D. W. Blackhurst, and W. R. Boone While seasonality alters sperm motility parat rs and these changes are not significant enough morphology, to alter pregnancy rates Selection of Embryos for Day-3 Transfer at the Pronuclear-Stage and Pronuclear-Stage Cryopreservation Results in High Delivery Rates in Fresh and Frozen Cycles D. G. Hammitt, C. A. Sattler, M. L. Manes, and A. P. Singh Selection of transfer embryos on day one I clear-stage ¢ preservation results in high fresh and frozen delivery rates and optimizes if . . Sherl Communication FSH Time-Concentration Profiles Before and After Administration of 0.25 mg Cetrorelix in the GnRH-Antagonist Multiple-Dose Protocol for Ovarian Hyperstimulation G. Griesinger, A. Alisch, D. Finas, K. Roiha, A. Schultze-Mosgau, A. K. Schréder, K. Dafopoulos, K. Diedrich, and R. Felberbaum Journal of Assisted Reproduction and Genetics CONTENTS Volume 21, Number 8 August 2004 283 Alpha Page —Assested Riyreductive i lechnelogy 285 Significance of Increased Endometrial Thickness in Assisted Reproduction Technology Treatments R. Yoeli, J. Ashkenazi, R. Orvieto, M. Shelef, B. Kaplan, and I. Bar-Hava Increased endometrial thickness (>14 mm) is not associated with decreased implantation o1 pregnancy rates in assisted reproduction 291 Prospective Randomized Comparison of Two Embryo Culture Systems: Pl Medium by Irvine Scientific and the Cook IVF Medium D. Ben-Yosef, A. Amit, F Azem, T. Schwartz, T; Cohen, N. Mei-Raz, A. Carmon, J. B. Lessing, Y. Yaron A comparison of two IVF culture media revealed that Pl Medium may be associated with a higher embryo cleavage rate and improved implantation rates than to the Cook IVF Medium 297 Exposure of Preimplantation Embryos to Platelet-Activating Factor Increases Birth Rate W. E. Roudebush, J. B. Massey, H. I. Kort, C. W. Elsner, A. A. Toledo, D. Mitchell-Leef, and D. B. Shapiro Platelet-activating factor (PAF) enhances live birth rates ‘ Vhiystoloy Y 301 =‘I n Vitro Effect of Cyclic Adenosine 3’, 5'-Monophosphate (cAMP) on Early Human Ovarian Follicles P. Zhang, H. Louhio, T. Tuuri, J. Sjéberg, J. Hreinsson, E. E. Telfer, and O. Hovatta Human ovarian follicles within cortical slices cultured with 8-bromo-cAMP showed promoted folliculogenesis and better follicle survival ¢ . *. Sherl Communications 307. ~—‘F reezing of Human Immature Oocytes Using Cryoloops with Taxol in the Vitrification Solution K. Fuchinoue, N. Fukunaga, S. Chiba, Y. Nakajo, A. Yagi, and K. Kyono 311 In Vitro Fertilization in Patients with Ovarian Endometrioma R. M. dos Reis, I. L. A. Corréa, A. Goncalves De Angelo, L. A. Manetta, M. D. de Moura, and R. A. Ferriani