IN VIVO IMMUNOLOGY Histophysiology of the Lymphoid System ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY Editorial Board: NATHAN BACK, State University of New York at Buffalo NICHOLAS R. DI LUZIO, Tulane University School of Medicine EPHRAIM KA TCHALSKI-KATZIR, The Weizmann Institute of Science DA VID KRITCHEVSKY, Wistar Institute ABEL LAJTHA, Rockland Research Institute RODOLFO PAOLETTI, University of Milan Recent Volumes in this Series Volume 147 INTRAOVARIAN CONTROL MECHANISMS Edited by Cornelia P. Channing and Sheldon J. Segal Volume 148 STRUCTURE AND FUNCTION RELATIONSHIPS IN BIOCHEMICAL SYSTEMS Edited by Francesco Bossa, Emilia Chiancone, Alessandro Finazzi-Agro, and Roberto Strom Volume 149 IN VIVO IMMUNOLOGY: Histophysiology of the Lymphoid System Edited by Paul Nieuwenhuis, A. A. van den Broek, and M. G. Hanna, Jr. Volume 150 IMMUNOBIOLOGY OF PROTEINS AND PEPTIDES-II Edited by M. Z. Atassi Volume 151 REGULATION OF PHOSPHATE AND MINERAL METABOLISM Edited by Shaul G. Massry, Joseph M. Letteri, and Eberhard Ritz Volume 152 NEW VISTAS IN GLYCOLIPID RESEARCH Edited by Akira Makita, Shizuo Handa, Tamotsu Taketomi, and Yoshitaka Nagai Volume 153 UREA CYCLE DISEASES Edited by A. Lowenthal, A. Mori, and B. Marescau IN VIVO IMMUNOLOGY Histophysiology of the Lymphoid System Edited by Paul Nieuwenhuis A. A. van den Broek University of Groningen Groningen, The Netherlands and M. G. Hanna, Jr. NCI-Frederick Cancer Research Facility Frederick, Maryland PLENUM PRESS • NEW YORK AND LONDON Library of Congress Cataloging in Publication Data International Conference on Lymphatic Tissues and Germinal Centers in Immune Reactions (7th: 1981: Groningen, Netherlands). In vivo immunology. (Advances in experimental medicine and biology; v. 149) Proceedings of the 7th germinal center conference, June IS-19, 1981, Groningen, Netherlands. Bibliography: p. Includes index. I. Immunology-Congresses. 2. Immunocompetent cells-Congresses. I. Nieuwenhuis, Paul. II. Broek, A. A. van den (Arie Adriaan van den). III. Hanna, Michael G., 1936- . IV. Title. V. Series. [DNLM: I. Lymphoid tissue- Physiology-Congresses. 2. Lymphocytes-Physiology-Congresses. 3. Antibody for- mation-Congresses. 4. Immunity, Cellular-Congresses. S. Cell movement- Congresses. WI ADSS9 v.149 I QW S68 IS9 1981iJ QRI80.3.IS3 1981 616.07'9 82-12283 ISBN 978-14684-9068-8 ISBN 978-14684-90664 (eBook) DOl 10.1 007/978-14684-90664 Proceedings of the Seventh International Conference on Lymphatic Tissues and Germinal Centers in Immune Reactions, held June IS-19, 1981, in Groningen, The Netherlands ©1982 Plenum Press, New York Softcover reprint oft he hardcover 1st edition 1982 A Division of Plenum Publishing Corporation 233 Spring Street, New York, N.Y. 10013 All rights reserved No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the Publisher PREFACE In 1966, the first Germinal Centre Conference was held in Bern, Switzerland, as a result of an initiative from C.C. Longdon of Oak Ridge, Tennessee and H. Cottier of Bern. Fifteen years later, in June of 1981, the 7th conference in this series was held in Groningen, the Netherlands. At this "International Conference on Lymphatic Tissues and Germinal Centers in Immune Reactions," instead of focusing on germinal centres only, the intricate relationships between various constituents of the lympho-myeloid complex consti- tuted the theme of the meeting. As Yoffee stated in 1966, "Perhaps we should consider not only the germinal centre itself but the surrounding tissue as well." Over the years, these conferences have brought together a small but dedicated group of scientists, sharing (1) a fundamental interest in the physiology of the lymphoid system proper, and (2) the recognition that, only in the in vivo situation can the final answers be obtained, however ingeniously in vitro experiments may mimic reality. In 1966, the conference lasted three days and included 57 contributions "which were discussed at length." The present conference accomodated 62 free papers and 64 poster presentations as well as some 24 additional abstracts selected from more than 200 submissions. Prominent among the selection criteria was the adage "in vivo veritas." This volume represents the presentations from the 1981 Germinal Centre Conference. At the meeting, the organizers restricted topics to those related to structure and function of the lymphoid system including the bone marrow. Thus, a compact meeting, where topics of interest to all participants were presented at plenary sessions, emerged. v vi PREFACE This formula of plenary sessions, as in previous meetings, seems to be largely responsible for the continuing success of these conferences. At this point, the organizers wish to acknowledge the very stimulating help of Dr. M.G. Hanna, Jr., of the NCI-Frederick Cancer Research Facility, Frederick, Maryland, USA. He was present at the birth of the first Germinal Centre Conference and since then has been the "auctor intellectualis" of every following meeting. It seemed only proper to end the meeting with a session_dedi- cated to "the still unsolved mystery of the germinal centre (con- tinued)," referring to the title of a paper presented by Dr. J.H. Humphrey, as invited speaker, at the fifth conference in Tiberias, 1975. To mark the occasion of his retirement and to acknowledge his many and outstanding contributions to this and previous Germinal Centre Conferences as well as to the immunological society as a whole, Prof. Humphrey was presented with the "7th Germinal Centre Conference Medal" which was especially designed for the occasion. Finally, some questions were answered and new questions arose at this meeting; all of which is part of the usual scientific process. It is our sincere wish, however, that at future meetings the con- geniality among participants, so characteristic of the last and previous meetings, will continue to accompany our progress in science. P. Nieuwenhuis A.A. van den Broek CONTENTS SESSION 1 BONE MARROW. B CELL DIFFERENTIATION, AND THE B CELL SYSTEM Chairman's Introduction: Bone Marrow and B Cell Differentiation 3 D.G. Osmond Higration of Erythropoietic and Prebursal Stem Cells from the Early Chicken Embryo to the Yolk Sac 11 Paavo Toivanen, Olli Lassila, Jussi Eskola, Claude Martin, Francoise Dieterlen-Lievre, and Douglas G. Gilmour Antigens Associated with Bursal and Thymic Reticular Epithelial Cells ••••• 19 I.G. Barr, M.R. Alderton, J.L. Brumley, R.L. Boyd, H.K. Muller, and H.A. Ward The Development of B Lymphocytes and Their Reactivity in Pig Fetuses •••• ••• 25 L. Jaroskova, F. Kovaru, H. Tlaskalova, I. Trebichavsky, L. Fornusek, M. Holub Regulation of B Cell Development in Mouse Bone Marrow • • • • • • • 31 P.P. Le Bouteiller and G.L. Asherson Distribution and Functional Properties of PNA+ and PNA- Cells in Central and Peripheral Lymphoid Organs of the Chicken • • • • • • • 39 K. Schauenstein, M. Rosenberg, A. Globerson, and N. Sharon vii viii CONTENTS Peanut Agglutinin (PNA) Binding as a Marker for Immature Human B Lymphocytes. Is Bone Marrow Not the Complete Bursa-Equivalent? •• 47 Ton Logtenberg and Gijsbert C. de Gast Functional Studies on Subpopulations of B-Lymphocytes and Bone Marrow Cells •••• • • • • • • • 53 A. Chayen, S. Marshall-Clarke, and R.M.E. Parkhouse Human Sessile B Lymphocyte Marker Defined by a Monoclonal Antibody •••• 61 H.K. Forster, F. Suter, F.G. Gudat, and J .P. Obrecht Marginal Zones: The Largest B Cell Compartment of the Rat Spleen • • • • • • • • • • • • • • • • 67 D.S. Kumararatne, I.C.M. MacLennan, H. Bazin, and D. Gray The Proliferative Activity of Antibody Forming Cells in the Mouse Bone Marrow • • • • 75 G. Koch, B.D. Lok, and R. Benner The Origin of Marginal-Zone Cells. • • • • • • • • •• 83 D.S. Kumararatne and I.C.M. MacLennan Functional Antigen Binding by the Defective B Cells of CBA/N Mice •• • • • • • • 91 H. Snippe, B. Merchant, E.F. Lizzio, and J.K. Inman The Role of Bursal Microenvironment in the Maturation of B Cells • • • • 97 Olli Vainio, Olli Lassila, and Auli Toivanen Fluorescence Activated Cell Sorter Analysis of Murine B Cell Development • • • • • •• 103 J.J. Haaijman and W.J.A. Boersma Pre B Cell Leukaemia in the Rat • • • 111 J. Rozing, L.M.B. Vaessen, L. Faber, M. van Oven, L. de-Vries-Box, B. de Jong, and P. Nieuwenhuis Differences in In Vivo Functional Capacities of Avian Precursor Band T Cells Following In Vitro Incubation ••••••••••• 119 W.T. Weber CONTENTS ix Lymphoid Antigens on Non-lymphoid Factor-Dependent Haemopoietic Cell Lines • • • • • • • • • • • • •• 127 J.M. Garland and T.M. Dexter SESSION 2 LYHPHOCYTE MIGRATION Chairman's Introduction: Experimental Approaches to Lymphocyte Traffic: Pitfalls of the Tracer Sample Hethod • • • • • • • • • • • • • • • • •• 139 W.L. Ford and M.E. Smith Two Populations of Hurine LPS-Responsive B Lymphocytes Differing in Recirculatory Capacity • • • • • • • • • • • • • • • • • • • • • • •• 147 Valerie J. \fallis and Honisha Chaudhuri A lfuthod for Studying the Dynamics of the Primary Migration of Human Lymphocytes Using Indium-III Oxine Cell Labelling 153 John Wagstaff, Chris Gibson, Nick Thatcher, and Derek Crowther LYT Phenotype and Lectin Binding Properties of Mouse Lymphocytes Which Enter Lymph Nodes 161 A.M. Carroll, Y. Reisner. and H. de Sousa Unilateral Localization of Hapten-Specific B Memory Cells in Lymph Node Draining A Footpad Injection of Antigen • • • • • • • • • • • • • • • • •• 167 Y. Baine, N.M. Ponzio, and G.J. Thorbecke Organ-Specific Homing of B Cell Hybridomas 179 P. De Baetselier, E. Gorelik, Z. Eshbar, Y. Ron, S. Katsav, M. Feldman, and S. Segal Isolation of Factors Causing Enhanced Lymphocyte Adherence to High Endothelial Venules In Vitro • • • • • • • • • • • • • • 187 Gregory D. Carey, Yee-llon Chin, and Judith J. Woodruff Lymphocyte-Endothelial Interactions and Histocompatibility Restriction ••••••••••• 193 A.S.G. Curtis and R.M. Renshaw x CONTENTS Selective Migration of Murine Lymphocytes and Lymphoblast Populations and the Role of Endothelial Cell Recogni tion • • • • • • • • • 199 Eugene C. Butcher, Georg Kraal, Susan K. Stevens, and Irving L. Weissman The Effect of Stimulated Macrophages on High Endothelial Venules and Germinal Centres in Lymph Nodes of Rat • 207 H.R. Hendriks, H.A.B. v. Hemert, and M. v.d. Heijden Homing Pattern of Newly Formed Splenic Lymphocytes •••••••••••••••••••••• 213 Reinhard Pabst, Ernst Nowara, and Karla Pots chick The Efficiency of Entry of Lymphoid Cells into Lymphoid and Non-lymphoid Tissues 219 C.A. Ottaway Morphological Aspects of the Local Immune Response to Influenza in Rats • • • • • • • • • • • 225 Zlatko R. Angelov and Ludmilla A. Angelova SESSION 3 THYMUS, T CELL DIFFERENTIATION, AND THE T CELL SYSTEM Chairman's Introduction: Thymus, T Cell Differentiation and the T Cell System • • • • • • • • • • • • • • • 237 E.J. Jenkinson Detection of MHC, THY-I, LYT-I and LYT-2 Antigens in the Developing Mouse Thymus • • • • • • •• 241 W. van Ewijk, E.J. Jenkinson, P.L. von Soest, and J.J.T. Owen . Role of Thymic ~ledullary Lymphocytes in the Intra-Thymic Maturation Process • • • • • • • •• 249 Martine Papiernik Sequential Development of THY-I+ Subpopulations in Regenerating Thymus After Bone Marrow Transplantation •••••••• • • • • • • • • • • •• 255 W.J.A. Boersma and J.J. Haaijman