A. Takada . A.Z. Budzynski (Eds.) Hemostasis and Circulation With 110 Illustrations Springer Japan KK A. TAKADA Department of Physiology, Hamamatsu University, School of Medicine, Hamamatsu, Shizuoka, 431-31 Japan A. Z. BUDZYNSKJ Department of Biochemistry, Temple University, School of Medicine, Philadelphia, USA ISBN 978-4-431-70096-8 ISBN 978-4-431-66925-8 (eBook) DOI 10.1007/978-4-431-66925-8 © Springer Japan 1992 Originally published by Springer-Verlag Tokyo in 1992. This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting,. reproduction on microfilms or in other ways, and storage in data banks. The use of registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Product liability: The publisher can give no guarantee for information about drug dosage and application thereof contained in this book. In every individual case the respective user must check its accuracy by consulting other pharmaceuticalliterature. PREFACE The First Polish-Japanese Symposium on Hemostasis and Circulation was held on August 8 and 9, 1991 at Hamamatsu Grand Hotel, Hamamatsu, Shizuoka ken, Japan. The meeting was officially supported by the Japanese Ministry of Education, Science and Culture. The financial support contributed by many industries and pharmaceutical companies made this meeting possible. The Organizing Committee of the Symposium was formed at Hamamatsu University School of Medicine under the chairmanship of Professor Shukichi Sakaguchi with Professor Akikazu Takada as a secretary. The First Polish-Japanese Symposium on Hemostasis and Circulation brought together scientists from three countries and provided a forum for promotion of scientific cooperation. The topics addressed at the Symposium are of interest and importance not only for participants but also for investigators throughout the world. A refreshing approach has been accomplished by joining problems of hemostasis with those of the circulatory blood, an interrelationship addressing pathophysiological circumstances. The Polish investigators have a long tradition in pursuing problems of hemostasis and thrombosis. Initially, Dr. H. Kowarzyk and then Drs. Buluk and E. Kowalski inspired and educated many investigators who working in Poland and abroad achieved international recognition. The recent political and economic changes in Po land are harbingers of progress and stabilization that will provide a better foundation for international scientific cooperation. A good example are several Polish investigators who came to Hamamatsu and participated in the first step in this direction. Finally, we would like to express our sincere thanks to members of the Department of Physiology of Hamamatsu University, especially Dr. Yumiko Takada, who helped to accomplish the successful symposium. August 21, 1991 Akikazu Takada Andrei Z. Budzynski Hamamatsu, Japan OVERVIEW Andrei Z. Budzynski and Akikazu Takada A variety of topics presented at the Symposium in 34 talks addressed current issues of hemostasis and circulation. These have been grouped into problems of interaction with thrombin, modulation of coagulation, platelet function, fibrinolysis, topics pertinent to circulation, and clinical aspects of hemostasis. Selected reports have been highlighted before to illustrate the scope of the Symposium. Thrombin catalyzes the conversion of fibrinogen to fibrin. The structure of a fibrin polymerization site in the gamma chain, that directs the formation of a clot, has been delineated after chemical crosslinking of a complementary peptide (Cierniewski and Budzynski). Specific degradation of fibrinogen in the blood wasdemonstrated to be the mechanism of blood anticoagulation by hementin, an enzyme occurring in the salivary gland of the giant South American leech (Budzynski). The cellular mechanism of plasma thrombomodulin regulation, involving among others cytokines and glucocorticoids, affects its level and may be of pathologic significance (Ohdama et al). Platelet functions are modulated by a variety of agents isolated from snake venoms, in particular by disintegrins, alpha-fibrinogenases, and thrombin-like enzymes (Teng and Huang). In an electron microscope study the storage of GPIIb/IIla in the alpha-granules and its migration and attachment to the cell surface skeleton, upon activation of platelets have been shown (Suzuki). Platelet aggregation occurs not only upon the action of agonists, but also as a result of shear stress at low or high veloeities of flow (Ikeda and Handa). As ethanol inhibits ADP-induced platelet aggregation, it has been shown that its effeet is mediated through serotonergic meehanism which is not assoeiated with the serotonin platelet receptor (Buczko). Regarding regulation of fibrinolysis, it was demonstrated that a peptide from the amino-terminal domain of Glu-plasminogen, eontaining -Lys-Glu- sequence, is important for the lysine binding site and enhanees plasminogen activation (Urano et al). Stimulated neutrophils seerete proteolytic enzymes affeeting platelet aggregation and clot lysis (Kopee). For example, single chain urokinase is inaetivated by neutrophil elastase (Kanayama et al); however, eathepsin B aetivates the soluble and eell reeeptor-bound form of the proenzyme (Kobayashi et al). Extraeorporeal eireulation in oxygenators, artificial dialysis devices, and during plasmapheresis exposes blood to thrombogenie surfaces; blood response is complex involving contaet activation of coagulation, complement aetivation, and eell adhesion (Matsuda). A novel direetion was attempts to assess the influenee of the eentral nervous system on eoagulation and fibrinolysis. For instance, induetion of a mental stress resulted in increase of eateeholamines and decrease of plasminogen activator inhibitor that led to activation of both platelets and fibrinolysis (Takada et al). Contents BASIC AND FUNCTIONAL STUDIES 1. PRIMARY POLYMERIZATION SITES IN D-DOMAIN OF HUMAN FIBRINOGEN C. S. Cierniewski and A.Z. Budzynski 3 2. MOLECULAR INTERACTION BETWEEN THROMBIN AND THROMBOMODULIN IN THE PROTEIN C PATHWAY K. Suzuki. 9 3. HEPARIN-BINDING PROPERTY OF HUMAN PROTEIN C T. Koide and Y. Kazama 16 4. A NEW THROMBOSIS MODEL WITH PHOTOCHEMICAL REACTION IN THE RAT FEMORAL ARTERY Y. Takiguehi. H. Matsuno. T. Uematsu. K. Wada and M. Nakashima 22 5. CONVERSION OF FIBRINOGEN TO THROMBIN AND ABSORPTION OF THROMBIN BY FIBRIN CLOTS--THE OBSERVATIONS AND A HYPOTHESIS C. Y. Liu. D.L. Wang and Y-T. Pan 28 6. LOW MOLECULAR WEIGHT HEPARIN IN THE TREATMENT OF DEEP VEIN THROMBOSIS OF INFERIOR LIMBS S. Lopaciuk 33 7. THE MECHANISM OF BLOOD ANTICOAGULA TION BY LEECH HEMENTIN A. Z. Budzynski 38 8. REGULATORY MECHANISMS OF THROMBOMODULIN EXPRESSION IN THE HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS. S. Ohdama. S. Takano, S. Miyake, K. Hirokawa and N. Aoki 42 9. LOCALIZATION OF HEPARIN-LlKE COMPOUNDS IN CULTURED AORTIC ENDOTHELIAL CELLS K. Takeuehi. K. Shimada, M. Nishinaga, S. Kimura and T. Ozawa 46 10. THE EFFECT OF HUMAN GRANULOCYTIC NEUTRAL PROTEASES ON HEMOSTASIS: EFFECTS OF CATHEPSIN G ON PLATELETS M. Kopee and K. Bykowska 52 PLATELETS 11. GL YCOPROTEIN IIb/IIIa AND MEMBRANE SKELETON IN RESTING AND ACTIVATED PLATELETS H. Suzuki. K. Tanoue and H. Yamazaki 59 12. MOLECULAR MECHANISM OF SHEAR STRESS-INDUCED PLATELET AGGREGATION Y. Ikeda. M. Handa , Y: Kawai and K. Watanabe 65 13. THE EFFECTS OF LIPID PEROXIDE ON BLOOD PLATELET SEROTONIN T. Hiramitsu and M.H. Pietraszek 71 14. LIPOPROTEIN(A) (LP(A»: ITS RELEVANCE TO DIABETIC ANGIOPATHY I Taminato, F.N. Hu. T. Tominaga, S. Sen, T. Yoshimi, Y. Suzuki. M. Nagao, Y. Takada and A. Takada 77 15. EFFECT OF ETHANOL ON BLOOD SEROTONERGIC MECHANISMS W. Buczko. B. Malinowska, E. Chabielska and D. Pawlak 82 16. SNAKE VENOM PROTEINS AFFECTING PLATELET FUNCTIONS C. M. Teng and T. F. Huang 88 17 . ALTERED PLA TELET RESPONSES TO THROMBOXANE A2 IN PA TIENTS WITH MYELOPROLIFERATIVE DISORDERS M. Okuma, F. Ushikubi. T. Ishibashi, S. Narumiya, K. Tomo and H. Takayama 94 FIBRINOLYSIS 18. EFFECTS OF VARIOUS N-TERMINAL PEPTIDES OF GLU-PLASMINOGEN ON KEEPING ITS TIGHT CONFORMATION OF GLU-PLASMINOGEN T. Urano, Y. Takada and A. Takada 103 19. INACTIVATION OF HUMAN TUMOR CELL PRO-UROKINASE BY GRANULOCYTE ELASTASE N. Kanayama, T. Terao, M. Schmitt and H. Graeff 109 20. CATHEPSIN B EFFICIENTLY ACTIV A TES THE SOLUBLE AND TUMOR CELL RECEPTOR-BOUND FORM OFTHE PROENZYME UROKINASE-TYPE PLASMINOGEN ACTIVATOR (PRO-UPA) H. Kobayashi. N. Kanavama, M. Schmitt, L. Goretzki, N. Chucholowski. J. Calvete, M. KrameT. W. A. Günzler, F. Jänicke, T. Terao and H. Graeff 115 CIRCULATION 21. HEMOSTA TIC DISTURBANCES IN P ATIENTS WITH OCCLUSIVE V ASCULAR DISEASE M. Bielawiec, M. Z. Wojtukiewicz, K. Krupinski. J. Kloczko, A. Bodzenta-Lukaszyk, A.Szpak and B. Dworak 123 22. THE INTIMAL NEOV ASCULARIZATION IN ATHEROSCLEROTIC PLAQUES OF HUMAN CORONARY ARTERIES K. Sueishi. M. Kumamoto. H. Sakura and Y. Ishii 129 23. OXYGEN FREE RADIALS INHIBIT Ca2+-PUMP ACTIVITY BY OXIDIZING SULFHYDRYL GROUPS IN RAT HEART SARCOLEMMAL MEMBRANE M. Kaneko, A. Kobayashi and N. Yamazaki 136 CLINICAL STUDIES 24. PERIPHERAL SEROTONERGIC MECHANISMS IN HEALTH AND DISEASE M. H. Pietraszek, Ko. Ohara, Y. Takada and A. Takada 145 25. INFLUENCES OF MENTAL STRESS ON FIBRINOL YSIS. PLATELET AGGREGATION AND AMINES IN PLASMA Y. Takada. M.H. Pietraszek, T. Urano and A. Takada 151 26. HALOPERIDOL KINETICS IN SENILE DEMENTIA M. Nishimoto. T. Itaya, Ko. Ohara, K. Miyasato and Ke Ohara 157 27. HAEMOSTATIC SYSTEM IN URAEMIC PATIENTS FOLLOWING DDAVP ADMINISTRATION M. Mysliwiec, J. S. Malyszko, J. Malyszko, M. H. Pietraszek, A. Azzadin and W. Buczko 163 28. BIOLOGICAL RESPONSES AT ARIT.. __ IAL SURFACES AND RECENT PROGRESS IN EXTRACORPOREAL CIRCULATION T. Matsuda 169 29. ANTITHROMBOGENIC BIOMATERIALS. POLY(VINYL CHLORIDE) WITH HEPARIN- AND/OR PGI2-IMMOBILIZED IN HYDRO GELS AND THROMBOMODULIN-IMMOBILIZED BEADS I. Maruyama, M. Akashi and T. Okadome 177 30. HETEROGENOUS RESPONSES TO V ASOACTIVE SUBSTA NCES OF CANINE SUPERFICIAL AND JUXTAMEDULLARY AFFERENT ARTERIOLES R. Furuya, K. Ohishi, A. Katoh, A. Hishida and N. Honda 182 31. NATRIURETIC PEPTIDE mRNA IN SHR AND WKY I. Tanaka, K. Komatsu, T. Funai. A. Ichiyama and T. Yoshimi 188 32. DETERMINATION OF RENAL ALLOGRAFTS BLOOD FLOW BY UL TRASONIC DOPPLER METHOD T. Ushiyama 194 33. PLASMA LEVELS OF THROMBOMODULIN AND ACTIVE PLASMINOGEN ACTIV ATOR INHIBITOR INCREASE IN DISSEMINATED INTRA V ASCULAR COAGULATION WITH MULTIPLE ORGAN FAlLURE H. Asakura. H. Jokaji. M. Saito. C. Uotani. I. Kumabashiri. E. Morishita. M. Yamazaki and T. Matsuda 200 34. SIGNIFICANCE OF PLASMINOGEN ACTIV ATORS AND PLASMINOGEN ACTIVATOR INHIBITORS IN HUMAN MALIGNANT LUNG TUMORS M. Nagayama. H. Hayakawa. A. Sato. T. Urano. Y. Takada and A. Takada 207