Habitat characteristics of two scorpion species, Liocheles australasiae (Fabricius, 1775) and Isometrus maculatus (De Geer, 1778) in Miyako Islands, Japan Kazusa Kawai February 2021 — No. 331 Euscorpius Occasional Publications in Scorpiology EDITOR: Victor Fet, Marshall University, ‘[email protected]’ ASSOCIATE EDITOR: Michael E. Soleglad, ‘[email protected]’ TECHNICAL EDITOR: František Kovařík, ‘[email protected]’ Euscorpius is the first research publication completely devoted to scorpions (Arachnida: Scorpiones). Euscorpius takes advantage of the rapidly evolving medium of quick online publication, at the same time maintaining high research standards for the burgeoning field of scorpion science (scorpiology). Euscorpius is an expedient and viable medium for the publication of serious papers in scorpiology, including (but not limited to): systematics, evolution, ecology, biogeography, and general biology of scorpions. 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For further details on the new ICZN amendment, see http://www.pensoft.net/journals/zookeys/article/3944/. Publication date: 11 February 2021 http://zoobank.org/urn:lsid:zoobank.org:pub:899DB2B0-E072-410F-9C0E-CE1D32D37391 Euscorpius - Occasional Publications in Scorpiology. 2021, No. 331 Habitat characteristics of two scorpion species, Liocheles australasiae (Fabricius, 1775) and Isometrus maculatus (De Geer, 1778) in Miyako Islands, Japan Kazusa Kawai The Japan Scorpion Laboratory, 4-7, Motomachi, Gifu-shi, Gifu-ken, 500-8185, Japan; email: [email protected] http://zoobank.org/urn:lsid:zoobank.org:pub:899DB2B0-E072-410F-9C0E-CE1D32D37391 Summary Scorpion surveys were conducted in the five islands: Miyakojima, Ikemajima, Kurimajima, Irabujima, and Shimojishima (Miyako Islands, part of Nansei/Ryukyu Islands of Japan). Eight individuals of Liocheles australasiae (Hormuridae) and 16 individuals of Isometrus maculatus (Buthidae) were observed in a total of 10 points on four islands, except Ikemajima. The localities of both species are recorded in this survey, focusing on altitude, distance from the nearest coast, and sunlight. The lowest temperatures in the habitat are also examined, and the cold tolerance of the species is discussed. Introduction The populations on Chichijima and Hahajima (Ogasawara Islands) are thought to be invasive (Takashima, Liocheles australasiae (Fabricius, 1775) (Hormuriae) and 1945). Geographic information suggests that the Ioto Isometrus maculatus (De Geer, 1778) (Buthidae) are the only population may also have been introduced. However, this two species of scorpions found in Japan, specifically in its species has not been seen in Chichijima and Hahajima in southern chain of islands, usually referred to as “Ryukyu recent years, and it is not clear whether it is still established Islands” in English literature. Both species have been there. historically recorded in the Ryukyu Islands (Iwakawa, 1906), We surveyed this species in the Miyako Islands and I. maculatus has been later introduced to the (Miyakojima, Irabujima, and Shimojishima). Ogasawara Islands (Bonin and Volcano Islands; Takashima, The subgrouping of Japanese Islands (Fig. 1) is given 1945). Both species have a very wide range of distribution below. outside Japan, with L. australasiae distributed from South NANSEI ISLANDS Asia to East Asia, Southeast Asia, Australia and Oceania Satsunan Islands, no scorpions recorded (Fet et al., 2000), and I. maculatus is thought to be the most Ryukyu Islands widely distributed scorpion in the world, be found in tropical Okinawa Islands, no scorpions recorded and subtropical areas worldwide (Fet et al., 2000; Lourenço & Sakishima Islands Cloudsley-Thompson, 2012). Miyako Islands The Japanese distribution area of L. australasiae is the Ikemajima Island, no scorpions recorded Ryukyu Islands (Iwakawa, 1906), and it has been Irabujima Island, L. australasiae (see Kawai, 2020a; this confirmed on 11 islands: Miyakojima, Irabujima, survey), I. maculatus (this survey) Shimojishima, Taramajima, Ishigakijima, Iriomotejima, Shimojishima Island, L. australasiae (see Kawai, 2020a), Kohamajima, Taketomijima, Kuroshima, Haterumajima, and I.maculatus (this survey) Yonagunijima (Shimojana, 1972, 2015; Takashima, 1942; Miyakojima Island, L. australasiae, I. maculatus (see Chigira & Tanaka, 2004; Tanaka, 2012; Karasawa & Shimojana, 1972; this survey) Kawazoe, 2005; Kawai, 2020a, 2020b) (Fig. 2). We surveyed Kurimajima Island, L. australasiae (this survey) this species in the Miyako Islands (Miyakojima, Kurimajima, Taramajima Island, L. australasiae (see Shimojana, 1999) and Irabujima). Yaeyama Islands Isometrus maculatus has been previously recorded from Ishigakijima Island, L. australasiae (see Shimojana, eight islands in Japan: Ishigakijima, Iriomotejima, Kamijijima, 1972), I. maculatus (see Takashima, 1948) Haterumajima, Yonagunijima, Chichijima, Hahajima, and Iriomotejima Island, L. australasiae (see Takashima, Ioto (Shimojana, 1972; Kawai, 2020b; Yokotsuka, 2011; 1942), I. maculatus (see Yokotsuka, 2011) Yamazaki et al., 2016; Muramatsu, 2007; Takashima, 1943, 1945, 1948, 1949; Kubo, 2001) (Fig. 3). 2 Euscorpius - 2021, No. 331 Figures 1–3: Figure 1. Location of Nansei Islands and Bonin Islands. Figure 2. Distribution of Liocheles australasiae (Fabricius, 1775) in the Nansei Islands. Figure 3. Distribution of Isometrus maculatus (De Geer, 1778) in the Nansei Islands. Kawai: Scorpions of Miyako Islands, Japan 3 Location Species Figure A Date/time Quantity Density Microhabitat D Kurimajima, L.australasiae Fig. 4 47 m 12/29/2020, 4 ♀ ad. 4 / fallen Under a bark of a 160 m 1 NE 17:30-18.00 tree fallen tree Irabujima, I.maculatus Fig. 5 70 m 12/29/2020, 3 ♀ ad., 1 / tree Under bark of 520 m SE - A 20:15-20:45 2 second Pinus luchuensis 2 instars and on the surface of a sign Irabujima, L.australasiae Fig. 6 74 m 12/29/2020, 1 ♀ ad, 2 / m² Under a bark of 380 m SE - B 20:15-20:45 1 ♀ sbad decayed wood and 3 in the grass Irabujima, S L.australasiae Fig. 7 59 m 12/29/2020, 1 ♀ ad 1 / m² Under a stone in a 730 m 4 12:00-12:15 cave Shimojishima, I.maculatus Fig. 8 10 m 12/29/2020, 1 ♀ ad 1 / tree Under bark of 35 m 5 NE 22:00-22:15 Pinus luchuensis Shimojishima, I.maculatus Fig. 9 13 m 12/29/2020, 1 ♂ ad 1 / fallen On a fallen tree 170 m 6 NW 22:30-22:45 tree Shimojishima, I.maculatus Fig.10 18 m 12/29/2020, 1 second 1 / branch On a branch in the 170 m SW 23:00-23:15 instar grass under 7 Pandanus odoratissimus Shimojishima, I.maculatus Fig. 11 7 m 12/30/2020, 7 ♀ ad 7 / fallen Under bark of a 960 m 8 center 11:30-11:45 tree fallen tree Miyakojima, L.australasiae Fig. 12 76 m 12/31/2020, 1 ♀ ad 1 / tree Under bark of 400 m 9 SE 01:15-01:30 Pinus luchuensis Miyakojima, I.maculatus Fig. 13 4 m 12/31/2020, 1 ♀ ad 1 / tree Under bark of 75 m 10 SW 02:10-02:45 Pinus luchuensis Table 1. The survey sites. Abbreviations: A – altitude, D – distance from nearest coast. Kohamajima Island, L. australasiae (see Chigira & Material and Methods Tanaka, 2004) Taketomijima Island, L. australasiae (Tanaka, 2012) In this survey, scorpions were searched for in Miyako Islands: Aragusukujima (Kamijijima) Island, I. maculatus (see Miyakojima, where both species are known to be distributed, Yamazaki et. al., 2016) and four surrounding islands (Ikemajima, Irabujima, Kuroshima Island, L. australasiae (see Karasawa & Shimojishima, and Kurimajima). Liocheles australasiae Kawazoe, 2005) has been previously recorded from Miyakojima, Irabujima, Haterumajima Island, L. australasiae, I. maculatus (see and Shimojishima but not from the remaining two islands. Kawai, 2020b) Isometrus maculatus has been previously recorded only from Yonagunijima Island, L. australasiae (see Kawai, Miyakojima but not from the remaining four islands. 2020a), I. maculatus (see Muramatsu, 2007) Sex determination for L. australasiae was done according to Senkaku Islands, no scorpions recorded the fact that genital operculum is divided into two in hormurid Daito Islands, no scorpions recorded males (Monod & Volschenk, 2004). The Isometrus maculatus OGASAWARA ARCHIPELAGO was considered a male if the pedipalp segments were long vs Ogasawara Islands (Bonin Islands) short in females (Kovařík, 2003). Chichijima Island, I. maculatus (see Takashima, 1949) The search continued from the evening of December 29, 2020 Hahajima Island, I. maculatus (see Takashima, 1943) to the early morning of December 31, 2020. On December 30, Volcano Islands there was a full moon. In the Miyakojima Island, December 29 Ioto Island, I. maculatus (see Kubo, 2001) had a maximum temperature of 23.0℃ (73.4℉), a minimum 4 Euscorpius - 2021, No. 331 Liocheles australasiae Quantity: 8 Sex: Female 8 Altitude Density Distance from the coast Point 1 47 m 4/tree 160 m Point 3 74 m 1/tree 380 m Point 4 59 m 1/m² 730 m Point 9 76 m 1/tree 400 m Average 64 m ― 418 m Table 2. Summary of records of Liocheles australasiae in this survey. Isometrus maculatus Quantity: 16 Sex: Male 1, Female 12, Juvenile 3 Altitude Density Distance from the coast Point 2 70 m 1/tree 520 m Point 5 35 m 1/tree 35 m Point 6 13 m 1/tree 170 m Point 7 18 m 1/tree 170 m Point 8 4 m 7/tree 960 m Point 10 4 m 1/tree 75 m Average 24 m ― 322 m Table 3. Summary of records of Isometrus maculatus in this survey. temperature of 20℃ (68℉), an average of 21℃ (70℉), and upon were found, including Porcellio scaber Latreille, 1804 was rainy. December 30 was also rainy with a maximum (Crustacea: Isopoda) (Fig. 4D) and Balta vilis (Brunner von temperature of 21℃ (69.8℉), a minimum temperature of Wattenwyl, 1865) (Insecta: Blattodea) (Fig. 8D). 11℃ (51.8℉), and an average of 16℃ (60.8℉). December 31 In addition, the lowest temperatures ever recorded on the was rainy and had a maximum temperature of 15℃ (59.0℉), island were examined, and the cold tolerance of these two a minimum temperature of 11℃ (51.8℉), and an average species was estimated. of 13℃ (55.4℉) (Table 4). Photographs were obtained with an Olympus Tough TG-6 camera. These photographs Discussion were slightly processed by the editing function in an Apple iPhone, only to optimize the brightness and contrast for print. Liocheles australasiae Illustrations were created using Microsoft Paint 3D software. Liocheles australasiae was found in Miyakojima, Kurimajima, The website of the Japan Meteorological Agency was referred and Irabujima Islands. It was the first record of this species to for the temperature. from Kurimajima. However, it was not found in Shimojishima, where it had been previously recorded, or in Ikemajima, Results where it has never been recorded. Eight individuals were found, all females. Only females of this species have been Scorpions were observed in a total of 10 locations on the four found in Japan thus far, likely due to the fact that this species Miyako Islands: Miyakojima, Kurimajima, Irabujima, and is capable of parthenogenesis (Yamazaki & Makioka, 2005). Shimojishima. In this survey, L. australasiae and I. maculatus Since males have been found in Australia, Papua New Guinea, were found in Miyakojima and Irabujima. Only L. australasiae and Thailand (Koch, 1977; Monod & Prendini, 2014), it is was found in Kurimajima, and only I. maculatus was found likely that Japan is not the country of origin, but it is unclear in Shimojishima. Liocheles australasiae has been previosuly when the species became established in Japan. There were no recorded from Shimojishima but was not found in this survey. individuals of this species giving birth, but there were adults Isometrus maculatus was searched for in Kurimajima but was whose bodies were slightly swollen, with late embryos faintly not found. In this study, Ikemajima was also surveyed, but visible; therefore, it is expected that they would have given neither species was found. Table 1 lists the altitude, date and birth approximately 1–2 months later. time of the survey, species recorded, number of individuals The altitude ranged from 47–76 m, with an average of 64 recorded, density (number of individuals recorded per tree), m, suggesting that this species is found in the environments that environment, and distance from the nearest port at each site. are located neither too low nor too high. In terms of density, Photographs of the environment and the scorpions found are approximately one individual per tree was recorded in most included in our figures. Although scorpion feeding was not places. However, the data also suggest that they are not actively observed during this survey, organisms that were likely preyed cannibalistic, as four individuals were found in a single tree at Kawai: Scorpions of Miyako Islands, Japan 5 Date Maximum Minimum Average Weather 12/29/2020 23℃ 20℃ 21℃ Rainy 12/30/2020 21℃ 11℃ 16℃ Rainy 12/31/2020 15℃ 11℃ 13℃ Cloudy Table 4. Meteorological data at Miyako Island on the day of the survey. Island Name Species Maximum Minimum Irabujima L. australasiae & I. maculatus 35.1℃ (8/20/1980) 5.1℃ (3/1/1987) Shimojishima L. australasiae & I. maculatus 36.1℃ (7/5/2016) 6.8℃ (2/14/2018) Miyakojima L. australasiae & I. maculatus 35.3℃ (7/16/1971) 6.9℃ (1/24/2015) Taramajima L. australasiae 35.4℃ (8/2/2009) 6.1℃ (12/28/2010) Ishigakijima L. australasiae & I. maculatus 36.1℃ (7/8/2012) 5.9℃ (2/19/1918) Iriomotejima L. australasiae & I. maculatus 35.7℃ (7/6/2014) 6.7℃ (1/28/1963) Haterumajima L. australasiae & I. maculatus 35.7℃ (8/5/2009) 7.8℃ (3/1/1987) Yonagunijima L. australasiae & I. maculatus 35.5℃ (7/23/2020) 7.7℃ (1/16/1967) Chichijima I. maculatus 34.1℃ (7/30/2006) 7.8℃ (2/8/1969) Table 5. Maximum and minimum temperatures at the habitats. the Kurimajima point of record. The distance from the nearest December to early January is considered the time when this coast ranged from 160 to 730 m, with an average of 418 m. species gives birth. The species was not present in the windbreaks on the coast, nor was it present in the center of the island. At Point 1, this The altitude of their habitat was low, averaging 24 m (4– species was located in the middle of the forest, and the ground 70 m range). The distance from the nearest coast ranged from was damp with little sunlight. Several P. scaber isopods were 35–960 m, with an average of 322 m. Excluding the value of found in the tree where this species was found, and a large 960 m (Point 8), the average altitude was 194 m, therefore number were found in the nearby leaf litter. This suggests that the species was found relatively close to the coast. Generally, this species was feeding mainly on P. scaber at the time of their density was one per tree; however, considering that the survey. In fact, when both species were brought back to seven scorpions were recorded from the same tree at Point 8, the laboratory and kept together, L. australasiae preyed upon it is likely that they are not actively cannibalistic. The females P. scaber. At Point 3, scorpions were found in the grass and remained still under the bark of the trees, even at night when rotting wood, and the ground was as damp as that at Point 1. they were active, but the single observed male was moving Point 4 was located in a cave, and the ground was moist. Point around the tree, probably looking for a female to mate with. 9 was a tree on the south side, which was a relatively drier At Point 2, I. maculatus was found on a tree and a sign along site, though on the northern side it was relatively more humid. a road to the south or west, respectively. On the day it was Based on the tree locations, this species appears to inhabit found, the area was wet from rain; however, during the day only humid places. it was dry from prolonged exposure to sunlight. At Points 1, 5, and 6, they were found in trees along roads to the south or Isometrus maculatus southeast, and at Point 8 they lived in decaying trees in sites This species was found in Miyakojima, Irabujima, and where there were no large trees around them. At Point 7, it was Shimojishima; this is the first record from Irabujima and found at a considerable height from the ground at a relatively Shimojishima. I. macultus was not found in Kurimajima or dry location. Thus, it appears that relatively dry habitats Ikemajima, from where it had not been recorded previously. are suitable for this species. Liocheles australasiae, which Sixteen individuals were found during this survey, of which prefer wet environments, and I. maculatus, which prefer dry only 1 was male, 12 were female, and 3 were juveniles whose environments, were not found in the same place, suggesting sex could not be determined. One female was carrying first- that they occur in distinctly different habitats. A Balta vilis instar juveniles on her back, suggesting that the juveniles had cockroach was found together with this species at Point 5. It is just been born. Another female was carrying second-instar likely that I. maculatus preys on B. vilis. juveniles on her back. The fact that second-instar juveniles On the day of the survey, the temperature had dropped were found in the same tree suggests that they were in the to a minimum of 11℃ (51.8℉). We reviewed the record low process of being separated from their parents. Several females temperatures on each island where the scorpion presence has had bellies swollen enough that the late embryos could be been confirmed so far (Table 5). The lowest temperature ever seen, indicating that they were close to giving birth. Late recorded on Irabujima, where both species are found, was 6 Euscorpius - 2021, No. 331 Figure 4: Figure 4A. Northeastern part of Kurimajima Island, location. Red point: place of discovery. Figure 4B. Environment. Figure 4C. A discovered female of Liocheles australasiae. Figure 4D. A discovered Porcellio scaber. Kawai: Scorpions of Miyako Islands, Japan 7 Figure 5: Figure 5A. Southeastern part of Irabujima Island, location. Red point: place of discovery. Figure 5B. Environment. Figure 5C–D. A discovered female of Isometrus maculatus with juveniles after the first ecdysis (the second instar) (C) and with newborns (the first instar) (D). 8 Euscorpius - 2021, No. 331 Figure 6: Figure 6A. Southeast part of Irabujima Island, location. Red point: place of discovery. Figure 6B. Environment. Figure 6C. A discovered female of Liocheles australasiae.