GenBank flat file: LOCUS DQ246664 319299 bp DNA linear VRT 03-NOV- 2005 DEFINITION Oncorhynchus mykiss SYPG1 (SYPG1), PHF1 (PHF1), and RGL2 (RGL2) genes, complete cds; DNaseII pseudogene, complete sequence; LGN-like, PBX2 (PBX2), NOTCH-like, TAP1 (TAP1), and BRD2 (BRD2) genes, complete cds; and MHCII-alpha and Raftlin-like pseudogenes, complete sequence. ACCESSION DQ246664 VERSION DQ246664 KEYWORDS . SOURCE Oncorhynchus mykiss (rainbow trout) ORGANISM Oncorhynchus mykiss Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Euteleostei; Protacanthopterygii; Salmoniformes; Salmonidae; Oncorhynchus. REFERENCE 1 (bases 1 to 319299) AUTHORS Palti,Y. and Hansen,J.D. TITLE Genomic sequence and annotation of a region that harbors major histocompatibility genes in rainbow trout (Oncorhynchus mykiss) JOURNAL Unpublished REFERENCE 2 (bases 1 to 319299) AUTHORS Palti,Y. and Hansen,J.D. TITLE Direct Submission JOURNAL Submitted (17-OCT-2005) National Center for Cool and Cold Water Aquaculture, ARS-USDA, 11861 Leetown Rd., Kearneysville, WV 25425, USA FEATURES Location/Qualifiers source 1..319299 /organism="Oncorhynchus mykiss" /mol_type="genomic DNA" /db_xref="taxon:8022" /clone_lib="Swanson 10X BAC library" gene <21292..>29844 /gene="SYPG1" mRNA join(<21292..21435,22024..22122,24025..24264,24519..24848, 26515..26659,26781..26925,27075..27311,27576..27777, 28094..28302,28612..28825,29224..>29844) /gene="SYPG1" /product="SYPG1" CDS join(21292..21435,22024..22122,24025..24264,24519..24848, 26515..26659,26781..26925,27075..27311,27576..27777, 28094..28302,28612..28825,29224..29844) /gene="SYPG1" /codon_start=1 /product="SYPG1" /protein_id="ABB52822" /translation="MQSFKESHSHESLLSPSSAAEALDLTLDEDAIIKPVHSSILGQE YCFEVTTASGTKCFACRSGAERDKWIENLQRAVKPNKDNSRRVDNVLKLWIIEARELP AKKRYYCELCLDDMLYARTTSKPRTDTVFWGEHFEFNNLPAVRNLRLHLYKETDKKRR KEKSTYLGLVSIPISSITGRQFVEQWYPVIQPSVLAKGQAGGKIINASLRLKSRYQTM SILPMELYKEFAEYVTNNYRTLCAVLEPLLSVKSKEEVACALVHILQSTGKAKDFLSD MAMCEVDRFIDREHLIFRENTLATKAIEEYLKLIGHKYLKDAIGEFIRALYESEENCE VDPMRTPPSVLPDHQANLRMCCELALCKIVNSHCVFPRELKEVFASWRVRCAERGRED IADRLISGSLFLRFLCPAVMSPSLFNLTQEYPDEQTSRTLSLISKVVQNLANFSKFGM KEEYMCFMNEFLEMEWGSMQQFLYEISNLDSVSNAGGFEGYIDLGRELSILHSLLWEV MAQLSKDAIIKLGPLPRLLNDISMALRNPHLQRQPSHQNQTDRVHERQTERLLSRPSF HRGVSSEFQNLMMKDLNSSIDITRLPSPTSGVSGGGVMPSRAHAQLSFQERDHPHRAS KDVFYVTRPPLARSSPAYCTSSSDITEPDHKASITGMGSFGGLSSQGGGGLGSGLSGQ LRAGGRHSAGSGGSSMSGGLRLSQLSQMGTTTDSLSQQQQQQAAALRYPLSFQNPLFH LATGTADGPHLHHQHSRAHLPPPLLLAPEPDPGGSHQAYIPQFAHGGFSRSEDLSTLR TGAGHLGQPAIIHSHSYSDDYSRADYARRQLSMHGQVWINLRSRTCLQSCSTFVQISL FVSPT" gene complement(<125842..>135286) /gene="PHF1" mRNA complement(join(<125842..126087,126545..126639, 127159..127245,127930..128011,128119..128197, 132494..132572,135202..>135286)) /gene="PHF1" /product="PHF1" CDS complement(join(125842..126087,126545..126639, 127159..127245,127930..128011,128119..128197, 132494..132572,135202..135286)) /gene="PHF1" /codon_start=1 /product="PHF1" /protein_id="ABB52823" /translation="MLRVQLRSAKFRQHWIAVVFAAVEYTVTDFSGDAEQHIYGYDTL YPCGSCTKLLRMGGVSEGDDVLARWSDGLLYLGNVKRVDGVKQCCLVRFEDNSEFWVL RKDIHSFSVGVEEVCCICDAPPLKEPLINCLKCRHGYHPECHTPSIEPEADSNSWICR QCVFAVATKFPSNVIYLECTDMTVSGEVAVVNRFVKIVFQRGGAIKRGRFARLMQIMK LRLPYQLSSLDWDPQHLTNQQQCYCYCAGPGE" gene <151383..>162236 /gene="RGL2" mRNA join(<151383..151508,153504..153587,153733..153878, 154091..154147,154314..154507,154818..154924, 155296..155511,156087..156316,157014..157098, 158294..158353,158716..158802,158925..158957, 159257..159378,160955..161048,161392..161616, 161848..161962,162058..>162236) /gene="RGL2" /product="RGL2" CDS join(151383..151508,153504..153587,153733..153878, 154091..154147,154314..154507,154818..154924, 155296..155511,156087..156316,157014..157098, 158294..158353,158716..158802,158925..158957, 159257..159378,160955..161048,161392..161616, 161848..161962,162058..162236) /gene="RGL2" /codon_start=1 /product="RGL2" /protein_id="ABB52824" /translation="MPACLPACLPACLPACLPACLACLACLPACLPACVSPLCVQQST VLEEKEDGVIYNVVVKQQHGAPNSATCVKAGTEEKLVQHLLHSFAMGDSSFINIFLST YRSFTSTKRVLDILIDRLQHPPGESTRSQTRQPFNRAVCMVFSMWLSEYPEDFRSLGE PGLLLRLAPLLPSDPSGTEIRGRLLRLAEELSEQALLPDSSSDRTIASPPADPTKFDP TNILGFPAGLIAEQLTKIETELFVRLVPYHCLGSLWSQRDKKGREGVCWSVRATVRQF NRLANAVMASCLWPTQLRSQQRACLLEKWISVAEVFMGEVTLGMYIVDHVRQRVPNNP IDRRQVFSDNAALCVFQQECRARKNFSSLYAIVSALQSNPVHRLRRTWLETDREAVRR YEELSNIFSEKDNYSQSRELLKEEGTSKFANLDGKMTNRRQIGSSAQGTVPYLGIFLT DLTMLDTAVKDRLENGYINFDKRRREFEVLAQIRLLQSSCKNCVFLTDEAFLQWYQSV PSLNEEESLNSSRTSLVADSDGLFDFPSPVCHFLSKFTKHMKSPSVSCLDVDSSPPTN DPIPSALTPSTPVKSHRRSVSCGNNPTNNNKGSEPDMRIVRIRMDLQDGNMYRSILVT SNDKTPTVISSALEKHSQDPKQASRYELIQLLPEGKELVIPATGNVFYAMTSSSVDFL LRRQGGNTPFRSQPISTETSATFPRIKAKGRRQLFPK" gene complement(164806..169585) /gene="DNaseII" /pseudo CDS complement(join(164806..165224,165383..165607, 168048..168206,168271..168382,168411..168613, 169012..169308,169495..169585)) /gene="DNaseII" /pseudo /codon_start=1 mRNA complement(join(<187423..187563,188211..188437, 188766..188925,189242..>189751)) /product="LGN-like" CDS complement(join(187423..187563,188211..188437, 188766..188925,189242..189751)) /codon_start=1 /product="LGN-like" /protein_id="ABB52825" /translation="MEVVMEYPGVKVETVEEEHLEDLIIIKEGDVERVVEGGTEPITG KSNDEDSSEEEDATARHGQIGKANTESGDGDKRKKDNQINQAKGETGTEEGVEHIKGM DELVMDRQKMECKKEGMVMVDITEKYKTTEHDLKTDKPSQGLQVGTNIHLPSDQLPKD TLSPEHAQKQAQRLTPYFPDALYDLVFTLQEGRRLNDQRCSFRGRRRCHSEPNTYIPA HRAHRVHFSSMTSLQKDEFFDLLATSQGRRLDDQRAELHDTPPPKPKANKKRSSVKDT KPKKSAPIAVQNEDLYNMILMSQAQGRLEEQRSAAPGPMDDEDFFSLLLSVQGGRMED QRTELPGILGT" gene <195665..>200086 /gene="PBX2" mRNA join(<195665..195713,197063..197307,197729..197919, 198662..198797,198898..199039,199736..199824, 200006..>200086) /gene="PBX2" /product="PBX2" CDS join(195665..195713,197063..197307,197729..197919, 198662..198797,198898..199039,199736..199824, 200006..200086) /gene="PBX2" /codon_start=1 /product="PBX2" /protein_id="ABB52826" /translation="MKPALFSVLCEIKEKTGLSMRNAQEEESQDPQLVRLDNMLLAEG VAGPEKGGGAAAAVSAATSSGGMSPDSSLEHSDYKSKLSQIRNIYHTEMEKYDQACSE FTTHVMNLLREQSRTRPVSPREIERMVAIIHRKFSSIQTQLKQSTCEAVMILRSRFLD ARRKRRNFSKQATEVLNEYFYSHLANPYPSEEAKEELAKQCSITVSQVCNWFGNKRIR YKKNIGKFQEEANIYAMKTAIVATQSEDSPHTPNSTGSGGFSLTGPDLFLGVPPMNGE QPVYMGAQTNGNWQGQNTPPSATSPGNDHSEESD" mRNA complement(join(<201034..201556,201672..202003, 202190..202287,203064..203165,203522..203657, 204158..204249,204438..204667,204777..205104, 206631..>206682)) /product="NOTCH-like" CDS complement(join(201034..201556,201672..202003, 202190..202287,203064..203165,203522..203657, 204158..204249,204438..204667,204777..205104, 206631..206682)) /codon_start=1 /product="NOTCH-like" /protein_id="ABB52827" /translation="MLVMWTAVLLGLSRCCQGHIHYCRDHYSNSYCDQGCESAACGWD GSDCHRHHSPLWAKGTLLLHTHVPLQHGTFSNSSLLWALSTLLQTPLKLRGTVPLDPS KDLFTFNPQQLENLLAQASSDDSNGSLLFLQVDNRPCSRLPSTCFPYAIEAANFLRAA TSSTRVSVPSHPELKAIISVRGVGEEIGGREEDPVEEKEDTNDGVTPPWIWAVIGVAT GLVLALVLIVVLVIRRDLDIGSDTDFTQSSMEDINRSICDHRTQEQKHYRSPPSHPQS PIQHGSVVLIRAVRSGLDRVVLELLRAGVPVNNTDHTGRSALHWACSVNHLSLTRTLT RYGAAVDLQDNKGETALFLSALHGCYDTARFLLLNGANQDLSDRRGRRALDVAREGMH HQVLELLLAHRVQRGPIPMEQANDMLWDEHAFLYSQWVNSAGLPGRSASFSGVIGHRD MSSPPPSDLSMGRVQCPSPQNWRPQLNQSATALVTPRIMGRPPRPISTLQEVTSEDED RERPQEVPRAATPHFLLPQPAPRQRSFSCTQNALQRRSSSQQPEPTYVALSEKIATEP IERVIVVPPTDAPTQSDRRSIVDSSDNPRRAAPEIEAASFKKAEQKARSEKLNNHMPD SNQTAL" gene <208782..>217102 /gene="TAP1" mRNA join(<208782..209099,209201..209508,209697..209827, 214116..214321,214649..214846,215129..215257, 215504..215692,215790..215963,216127..216286, 216517..216653,216875..>217102) /gene="TAP1" /product="TAP1" CDS join(208782..209099,209201..209508,209697..209827, 214116..214321,214649..214846,215129..215257, 215504..215692,215790..215963,216127..216286, 216517..216653,216875..217102) /gene="TAP1" /codon_start=1 /product="TAP1" /protein_id="ABB52828" /translation="MPKMNFSPLLFLCMDVCVVQTVRLAQLSPLLLHHPLITLWGGSL LRAGLHLFLTFKFPGSTPWMSSFEGLQSMGVLCFHCPLYISLLWACGQSILGQLWGWH SWQGLLQGYCVTVVACLYWTRYVPSLLTKPTKEPQKKTGASLEKLMGYMKPYFIRFGA VLSLVVISSLGEMAIPHYTGRMTDWIMNEDEPEAFTHAITVMSLITVMSAVCEFVCDL IYNITMSRIHTSIQGLVFQSVLKQEIAFFDTAQTGDLVSRITTDTNDMSESLSEKLSL LMWYFMRVTFLFIFMLNLSWKLSLFTAMGLPIIWVIPKISGKWYQELGAKVQKSLAQA NDVATETFSSMKTVRSFANEEGETERYRKRLEKTYSLNKEEAAAYAASTWTNSMSSLF LKVSILYYGGSLVTGGTVSSGDLVAFVLYELQFSSAVEAVMSYYPSVMRAIGGSEKIF EYVDRQPQVPPEGTLAPQNLEGHVEFKNVTFTYPTREDTPVLKGLSLELRPGQITALV GASGAGKSTCVSLLERFYLPQEGEILLDGQPLHSYKNQYLHDKISVVSQEPVLFARSV KENIKYGRDDATDEEMYRAAELANAHKFISDLPNGYDTDAGEKGGQVSGGQKQRIAIA RALIRKPRILVLDDATSNLDTESEHLVHQALLKDSNPCSVLLIAHKLSTVEKANHIVV LEEGKVLEEGSHVELLEKGGTYADLVNKQNTGFQRKEGEELKN" gene <223073..>228145 /gene="BRD2" mRNA join(<223073..223327,223542..223679,223843..223984, 224198..224355,224527..225036,225255..225383, 225727..225978,226412..226686,227211..227536, 227796..227921,228018..>228145) /gene="BRD2" /product="BRD2" CDS join(223073..223327,223542..223679,223843..223984, 224198..224355,224527..225036,225255..225383, 225727..225978,226412..226686,227211..227536, 227796..227921,228018..228145) /gene="BRD2" /codon_start=1 /product="BRD2" /protein_id="ABB52829" /translation="MEHHVSGPGKRMRKPSLLYEGFESPQLPHAPPPPPVQPPVRDPG RQGCMTNQLQFLQKAMIKSLWRHHFAWPFHEPVDAFRLNLPDYHKIIKQPMDMGTIKK RLENNYYRSASECMQDFNTMFTNCYIYNKPTDDIVLMAQSLEKVFLQKVAQMPQEEIE LPPPAPRGKPGKGGRKSNMWKAHQVPAVSQSAYSPSSSDTGDSDTPPQTMLPKSLPPT QLLSLHPTLPTAKKKGVKRKADTTTPSNMMTMPLSVGGGRMVGLGKGGHGQGLSHDPS TLCLTSLGDMDPPPSLVKGPMGPGGPVLLQPMIAGGGKTLARRGGSGRPIKPPKKDLP DSVQPKAPRRAKLSQQLRYCNSVLKDLLSKKHAAYAWPFYKPVDASALGLHDYHDIIK CPMDLSNIKRRMDSREYRDSQQFSADVRLMFSNCYKYNPPDHDVVGMARKLQDVFEFR FAKMPDEPLQQAPPKSRGMGGSMGTHAHGGGDSSSSSSSSSSSSESEPSSESESSPSS DSEEERAHRLAELQDQVCTQLRAVHEQLAALSQGPIVKNKKKREKKDKKEKKKKKKPE KRSRGRPSAEIGGEDWEMPTAKPMRTPKSKSSSRSSAPASSQGKRGPGKKKGSKNTKK SQAATMPYYASPTFSMLPHYDSEEEEETSPMSYDEKRQLSLDINKLPGEKLGRVVHII QSREPSLRDTNPEEIEIDFETLKPSTLRELERYVMTCLRKKPRKPYVKKGGPGKSREE LTLEKKRELERRLQDVSGQLNSGKKPQKPKAEKLSGVEPHAQPSRLSGSSSSSDSSSS SSSSSSDTSESDSG" gene 235220..246752 /gene="MHCII-alpha" /pseudo CDS join(235220..235236,236170..236341,237249..237510, 241720..241897,241996..242374,246552..246752) /gene="MHCII-alpha" /pseudo /codon_start=1 gene complement(266122..292969) /gene="Raftlin-like" /pseudo CDS complement(join(266122..266341,268551..269303, 269549..269677,270109..270296,274319..274437, 279732..279898,280065..280251,280606..280905, 283832..283951,284018..284303,285974..286082, 291119..291296,292885..292969)) /gene="Raftlin-like" /pseudo /codon_start=1 ORIGIN 1 acacctgtat ttggggagtt tctcccattc ttctctgcag atcctctcaa gctctgtcag 61 gttggatggg gagcattgct gcacagccat tttcaggtct ctccatagat gttagatcgg 121 gttcacgtcc gggttctggc tgggccactc aaggacattc agagacttgt cccaaagcca 181 ctcctgcgtt gtcttggctg tgtgcttagg gtcattgtcc tgttggaagg tgaaccttcg 241 ctccagcctg agatcctgag tactctggag caggtttttg tcaaggatct ctctgtattt 301 tgctcagttc atctttgcct tgatcttgac tagtctccca gtccctgccg ctgaaacaca 361 tcgccacagc atgatgctac caccatgctt caccttaggg atggtgccag gtttcctcca 421 gactttacct tatcgttggc attcaggcca aagagttcga tcttggttta atcagaccag 481 acaatgttgt ttctcatggt ctgagagtcc tttaggtgcc ttttggtaaa ctccaagcag 541 gctgtcatgt gccttttact gaggggcttc cgtctggcca ctttatcata aaggtctgat 601 tggtggaatg ctgcagagat agttgtcctt ctggaagatt ctcccatctc cacagaggaa 661 ctctagagct ctgtcagagt ggccatgggg ttcttggtca cctccctgac caaggccctt 721 ctcccctgat tgctcagttt ggccgggcga ccagctctag caagagtctt ggtgggtcca 781 aacttcttcc atttaagaat gatagaggcc actatgttct tggggacctt caatgctgca 841 gacatatttt ggtactttcc ccagatctgt gcctcgacac aatcctgtct cggagctcta 901 cggacaattc cttcgacctc aaggcttggt tttttctcta acatgtactg tcatttgagg 961 gattccaatt cagtccggtg cggtcttcca gacagtgcag ttgtgcttca agtgtgctct 1021 ctgtgtgatg acttgtacgt cggttgtgcc tacatggagt actgtcattt cgagtctcat 1081 atgcaaagtt tttaatgatt tataaatttg cacaaattta taaaaacctg ttttcctttt 1141 gtcattatgg ggtattttgt gcagattgct gaggattaaa aaaagaacac attttaaatc 1201 aaggctgtaa tgtaacaaaa tgtagaaaag gtcaaggggt ctgaatactt tccaaagaca 1261 ccatccagcc tggcctcata gactagatgt aaaagttaac aaaaatctgg tacactcaaa 1321 ttagtatgat atggtatgtt tggtatggtt acatcaggcc gaaggttatt taaggcaaaa 1381 attaaaggag ggtagttggt cggtgtggat ggttgagcga atagctactt tgcaactaca 1441 tagcatgtta gctaacactt cccctagccc tttcacctaa ctcctgacct taaccttaac 1501 ccctagccta gttaacatta gccacttagc taacgttagt gttagccacc tacttaacat 1561 tagccacaac aaactggaat tcttaacata tcatacgtat tgcaaatttg tagcatatat 1621 tatgaattgt aatatatcat acgaaatgga tgatggacat ccacatatta atacatgcca 1681 taccaaacat aacgatcata ctaatttgag tgtcccagat ttttgtttac tatgttatgt 1741 ctacccctga ctctgggttg atatttcagc tccagcattt attgattttc aattacagta 1801 aagctcgaca ttattcaaat tgctgttgac aaaatcaaaa taaccaaaat aatgtgaaca 1861 ataggtaaag aaagaaagaa tgctgttgat aatacgtgtg attttctgta aaatgtctgt 1921 gtaagtcaat aagtgcttct gttggtgctg catacggtca gtgacgcaca tacagtacag 1981 tggaggaatc gaagtgaaga ggcagaatgt atggatggag agaggcagta ccactgaagt 2041 gtatgctggt tggtttatta cctttgtact cggggtggaa cgggcgcgag acggggctgc 2101 actaggatgg attggcgcct cccctaagag aaatcaccgt atctcatcaa cacaggagaa 2161 gaggagagga acgatagaga aaaggaggag aggagaggct tgtgtgcact gaagaaagta 2221 aaaggtagaa gttgggtgtc gagggttgag gagcattagg gatactcaac gcccacagga 2281 cacacacaaa cacaaaggaa cacacaccac cattcgcgcc aacacaaacc cagcagcctg 2341 aaagcacaag agggcccaac atgtcaacca agtctgcatg ctgaggaagc aaaccaaggc 2401 tgttgataca cacatgcagc agtacagatg gtaagtagcc atcttgtgca agccagaaca 2461 tgaggcagct tgatgtacaa gtacaccctc tggacatgat gctactctat cgcagggcca 2521 tagcccccaa cctatctcct taacgctgag ccaagcagag acacattggg tcccgttttt 2581 agtctttgta atgactcggc ctgggatcga actcccaact ttccaatctc agggcggaca 2641 ctgacaacaa ggccactgag ttggtttggc agtaaagata gctgcatttt aattcaagct 2701 aaatgtcatt cattgatcca aagattgaaa agtcttgtcc agacaaatgg tatctctgat 2761 ctcctctgaa gctgaggggt tagaagttga ggcaaaaggt catgctactg ctaaacatgc 2821 agtaatcatt ggcaaaagta ataaacacca ctgttactgc aacaaggaat aaatagtatt 2881 aataaacaga gttaaacaat ataaatatgc atacacatgt aaaaaaaggt tgaatgacac 2941 ataacacact taaatacata cagggttata gagacagtag ataactgatg tgaaagagat 3001 aaccatgtaa agtgagacat gcattatatt cacaatgatg catggaacat ataacattaa 3061 gttggagagg atatgtacag tacaacatcc aaatgatgat ttatttgtta tgagtcattc 3121 caaggacagg ctgatccact gtactttcaa gagtgctacc actagttgct gcctacaggt 3181 gtaatatatt aattggaccc gtttctcaca gcaggaaaat aatcctgcac caacaggaat 3241 gtggataata attaatggat atgtttgtag gggttgatac atttttcaat agggcaaatc 3301 aagtcaaaca tttaaagtgg aaaatacaaa acgttagaag ctttataaac cctcaaatac 3361 aaacaagttt gcatttcctg ctgaacagga aaattctctg ttacaacaga ttgatcaaat 3421 taagatagaa catttgtata aacggtagta ataaaaacaa gcaatattat tacattaact 3481 aatttaatca agtttgacaa tgaaatccca ttaaaaccag atatttagta gagagggggt 3541 ggaggtattc tcaacataat gctgtaggaa tatgaatcca caaattccac gcaaaaagct 3601 gaaagccgag tgaactgccc actgttggga taataaagat aacgtgaacg tgaaccctat 3661 tagtcaccga cacccccacc tagacacccg gcattaaaca catgttgctg ccatggcgat 3721 ggacaagtga ttgtacggct cactgcaaat aatgacagat atttgaaacg caatcattaa 3781 aacatgccga catgctctcc ttgggcatag ccatgatggg aatgtaatgg tacacaaaca 3841 attggcccac aacaatttat ggcttcattt tataatctta ttgactataa ccttcagaac 3901 aatggagata cacagaaatg ttcgcgtggt tgcatgataa tggacaatta acatgagata 3961 aaagaaaacc tgggtggaag catgacatga aaataatgaa tgtcatcaga agttccatgt 4021 tggactgctg tgatttaata ctgtatctag gaagagaccc aaacagcttc atgttggata 4081 gcaaatctgc ctctttcagc ttggaaacaa aaccaatgtc aagatggatg cccagccttg 4141 gatgctccta cgaccacttg ggaagagcta ctcaaactat atctctcagc cctcagaaag 4201 aaccaataga tttcgattcc aatgctgtct gaggctgtag gccaagtcta tcatgacact 4261 ccctcaacta catgtcaacc acagactggc cacgttcgat cgagtgaaac cagtgcgagc 4321 gcaaaactct ggtctgctgc ttgtctggca actgaacaca acgccacgca tcgctggacg 4381 gctttacccc ctttcaacca cagtttctaa gcctttctgg ctaatctctc cctctccctc 4441 tccctccctc ccttcctctc cctcaccctc tctctctctc tctctggggg agctgtgtac 4501 tgaatggaga aataacagga gagacacatt ggatcactgc tcacttgagg aagcgtggct 4561 gaagccctga ctgttcgaca ccttgcaggg gtgctgcatt cagctgcatt cttatctggg 4621 acatagcggg agccctgtct gttcacaccg ctcaaacttt ctgcttttgt ttttatctct 4681 gcatgtcaga aaatgtctgt tgcctctatt gagtctgatt tgactcggat tgggataaag 4741 acatttggat tggggaagtg gcagggtggg actcagtgaa gcttgagcgt acatgtgtca 4801 tgttgtttgc aagagacatt gtggtttgac acatactcac tctctctcgc acattctttt 4861 acacacacaa acacacacac acacacacac acacagaaat ctggcatgtc tttgcagcat 4921 gactcatgat tgagggtggg cttgaccggt ttgacaggag atgatgggtg gctggctaaa 4981 agcatggacc gagagacatt gagtgtggat gctgaagacg ttgtcggcct gacaaatgaa 5041 ctcatcctca ggaccaaaac tgttcaatag tcgtgtatta tgtctgtctt gcatttgtgt 5101 tagactggta atacaacttg acgttgacat gctcagtctg aataattggg aggaaggagt 5161 ttggattgag gaagcggttg ggtgggactc atcttctggt ggaacaggga tttgatttaa 5221 acgaatgaaa gaagcttgag catacatctg tgatgtcgtt tgtaagagac attggggttt 5281 gacacactct gtctctcttt cacacacaca cacacacaga cacatacaca cacacacact 5341 gaaagctggt atgtctttgc agcatgactc atgcttgagg gcgggcctga cgagttcgat 5401 aggagatgag tggctggcta aaagcatgga ccgtgaaaca ttgagtgtgg atgctgaaga 5461 cgttgtcggc ctgacaaatg aactcatcct caggaccaaa actgttcaat agttgtgtat 5521 tatgtcttgc atttgtgttg gactggtaat acaacttgat ttaatacata tagttcacac 5581 aaagttgata gacgtgtctg agatgtacag ttactacaga cgctgatttg attacaaccc 5641 ggatgtggtc agacagaata taaacagatt gattgagaga cagacacaag atgtaaacag 5701 acgttctttg accttcaaag cacctaagcg gattgacaga caaatgagtt tcacccaaac 5761 ccaaggactt gtctggacga aataaaaggg acaaatcaac aaagagatga aaacaacata 5821 gagaaggata gagagataaa gacacaggcc atcctacaga catggcgacc ggaagaagaa 5881 aatcacagac ctgatcggcg aagaccctaa cgttagcttt tggcggctca accacggctc 5941 ctccccttat ctcatcctcc acagccataa gtctgagaga gagagagtga gagagagaga 6001 gagtgagagt tcaaacagtt gtgagagaat tgtgagtaaa ctagacaaga agtagctaag 6061 tgtaatacgt aaggcttata atgactgtcc aggtgcaagc tggatcgctc attgccctga 6121 aaaacgttga tttataccca ttacctggtc tatagtagtg ttgttaaaac ctctggtagc 6181 caaattataa tctattcaac caatgctgta actgtttaac atacactgct ctagtaatgc 6241 gaggaagcat ttttgaggca gttgaagtgc atgtttccat gttccccaag ggattcagca 6301 tgatgtgtct gtccaattca gggtgttttc caatgtaatc atgcctaatg atgtctagat 6361 tgtggtgttt tgccggttat cgtgttctga atggggttta attagcaata catcctcatg 6421 gaatagttga actgacacaa catgatagag attccaaatc cttgttacaa ccatttcaaa 6481 gagtcgtctt gcagggatat ttggaagctt attgctacag tctccatagg atatttggaa 6541 gcttattgct acagtctcca taggatattt ttcttgcctt gcaaattcac atttagacac 6601 gattactcag catcagaatt attttccaga gaagggaaca tggatcatta aatacttgcc 6661 tctggaattt caagatctgg gtcattcaag gcaatggttc tgatattttc ttcaacagac 6721 acaaaaggtg aaggatgaat taaaagtcac tgcaatcatg aggtcaaatc caagatggcg 6781 atcagctgtt tggtgatgga gattcaatga acaagggtac atatgacatc ctaactccta 6841 cctggtcaat atttctgagg taatgaaatt cttctgcttt gctcattata gatttcattg 6901 tcttcaatct tatcacatct actttccgcc tgaatgacat ggtggttttc atttaaagtc 6961 atacagaaaa gctgtgaaaa attgctgaca acgtggatga acttcagtgt taaactcctg 7021 agatgaacag aatgctgaga gaggactaat tacacaaaag gaatgactca ttttaataca 7081 gtgccttcaa aaagtattca cccccctttg actttttcac atttaaaatg gattacattg 7141 agattgtgtg tcactggctg gggttacccc ataatgtcaa agtggaatta tgtttttaaa 7201 catgttaaca aattaattaa aaattaaaag ctgaaatgtc ttgagtcaat aagtattcaa 7261 ccccttcgtt atggcaagcc taaataagtt caggagtaaa aatgtgctta agaagtcgca 7321 taataagtta catggactca ccgtgtgcaa taatagtgct taacaagact tttgaatgac 7381 tacctcatct ctgtacccca cacatacaga taattgtaag gtccctcagt tgagcagtga 7441 atttcaaaca cagattcaac cacaaagacc agggaggttt gtcaatgcct cacaaagagg 7501 ggcacctatt ggtagatggt aaaaaaaaag cagacattga atatcccttt gagcatggtt 7561 aagttattat tacactttgg acagtgtatc aatacaccca gtcactacaa agacacttcc 7621 taactcagtt gccggagaag aaggaaaccg ctcagggatt tcacgatgag gccagtgact 7681 ttaaaacagt tatagtttaa agactgtgat aagcgaaaac tgagactgga tcaacaacat 7741 aaaataaaac tgcaaaaaaa tgtgccaaag aaattaacaa ggcaaatata cacgggagtt 7801 gcttaccaag acaacattga atgttcctga atggcctagt tacatttgcc ttaaatcggc 7861 ttggaaatgc aagacttcaa aatagctgtc tagcaacaat caacaaccag cttgacagag 7921 cttgaatttt ttttataata atgtgcaaat gttgtgcaat ccaggtgttt cacagctgta 7981 atcgatgcca aaggtgacta acatacagta ttttgactca gggttgtgaa cacttatgta 8041 aattgcattt ctgtatttca atacaatttc taaaatagtt ttcactttgt cattgtgtgt 8101 agatgggtgt gattttttta aatacatttt ttattcagga tgtaacacaa caaaatgtgg 8161 aataagtcaa ggggtatgaa tactttctga aggaactgta taggacattg agcagtggca 8221 acagagaaag tgaggaccag acagccgatg tgattggtgc aaaaaaactg agccaataag 8281 ataagctaaa aacagaaata gcacagattt tagtcaaact attccaaaac gagtggttgg 8341 tttggacaga ggataacata tagtcatttt ccaccataat caagctgtca caattccgca 8401 gagaaagcat tctctgaata tctttatgct taacatacaa ctaaattgat gcttaacatg 8461 aataaataat gaaaatccac aggtgaagtt tcaaacattg gtgttagatg ttttattaca 8521 taaataatgt acagggattt atttgacaaa aataagaaag ggaaaaggaa tcaaacgtat 8581 gcactgactg aaacattggc actgatctaa gaaacaatga aatgtaacat gagtatacag 8641 tagaattcca aaaaaggcag acagtgattc acatgggtga tatgtaccaa aactaaagct 8701 gtatatctat ttacttcaag ttcaagtagt gtgcttgtca ggtcaagtct tacacgcagt 8761 caaaacaatg tgggtacttg tcaatatgag gtcagggaca ggtcatgata tctgacttgt 8821 ctgttacata gaaataaggc aggcgattgg gtgagtcaaa aggtcacatg atatgtagtc 8881 aattgtatgc acatcaacag taaactctca ctatgtgctg ctatttgcca tgtcaatcca 8941 aatgacttta ttgcacagtg acaagttata tatctctatg aaaactttaa aagtaatact 9001 gatgtaatat tatacatcta tacagactac caatattata cttgctacaa tgccaaacga 9061 gaggcaatct gatgatagtg aacaattctt gttccacatc gtatctcaaa aaatgactcc 9121 ctcaaagctc taactttagc aaacaatgac gccatttacc cttcggtttc ctatgactta 9181 ctgcagatca aaacttaaac caacgacata cagcaaaatt ataacagata tataaatctt 9241 aacactttag ataaatggca ccaatatcca tctggctaca tgattaacat tttcgttcaa 9301 agacgctata cagccatgtg taagtcaaag atataaatta tacattattt ttttacagta 9361 ctgtatatgc tgcatctatg gtatttaggg atttacagga tccatggtca tacatgcaga 9421 agtctcaata taatgtatgt taatatgggt tcatttgata ctgaaaggga cctgaaaaaa 9481 acctttaagt gtatgagttc tgtatgtata gtcatatgtt gtatctatgc atgtaaggtt 9541 tacatactca gtatctttgt gtgcctgaat gtaggcctac atacatatac ggattgcttc 9601 tgttcataaa tgctaaatgt agtctattat tgcacatctc gtaatgctaa aatattgcta 9661 tttcaataga ttaaaatagt taatttcatt ggtaatatca taacaaaatg tacatataga 9721 atcttagagt atccttattg gtatggcagt actggtgctt cagtggggta ttgataacat 9781 ttagaaaaag tgtgttactc tagcgtgtct gatattcttt gatatcatat aatctgtcat 9841 attcctctta agaaacataa ctgctactct agaggcacca agataaatca cacaaaaaaa 9901 aggctttcaa agaatatctg aagtcgtttc taagaagaat ggtgaatgag gtttgaaaaa 9961 caatgataga cgtttgggtt ttgtgaaatg tggaatgtat ggttaattat ccttttggag 10021 gacagtggat ataaccactt gagccactga aattgcttga aacatacttt caatgataaa 10081 taaaaaaaac gctaatagca tctcattcat tgacagcata tggatctgtc ttaaaacctt 10141 gcatccatcc actacttaca gaatcctcta gatgaccttg tcaactgtaa caatgccatt 10201 atccaaacta acttcaaagg ggaaggagaa cttgtcattt aaattaggtt tactctgttc 10261 tctttacaca tcatatgtta gggaaatact atcaaactgt ggttgcagtt tgtaaagatg 10321 gtattgttca tcctcatgtc aagctgttat ggtgatgaca acaagatgtc atgtactacc 10381 ttagtctttg tttcatgcat tttttatcaa aaacaccatt atagcctcat actgtatagg 10441 acagatagaa gtcatatgat gtcacctttc agtccattat tgtgcttttc aattcaatcg 10501 aggtgaatcc ctctcagttt acggttatcg gaataatcac cacagatgac gcaggtaaac 10561 tactgatgga aaaaaacaag catcatgttt gatatactgg caataaaatg gctgccaaat 10621 agtgcacaaa gggcctaaac atgtattttg ggttctttat gcaccaagtt atggggttat 10681 ggatgggatt gcttccatta agtacagcca ggaactgtct cggtcttcat tcgatttctt 10741 cttccctgga atggattaaa agtagcaaag ttccctgtga cacatatcta actatcaatt 10801 gcagattatt agaagtggtg aacaaaagca gaacatttac acaaatagtt tcctctctca 10861 agttcctcat ctcaagtcaa tttgtcaacg ttagtcagaa tgttcaatga acattttcct 10921 actgactggg tgaaaacaaa ttgcctaagg tatctgcata cagacatgaa tcattcatat 10981 gaatggattt cactggtgac ctctcacctt tgaaatgaat gtgcagttat tttgaagctg 11041 tcccattata tatatacact gctcaaaaaa ataaagggaa cactaaaata actgaatgaa 11101 tgaaatattc ttattaaata cttttttctt tacatagttg aatcacacac ctattatcaa 11161 tggaaatcaa atttatcaac cgatggaggt ctggatttgg agtcacactc aaaattcaag 11221 tggaaaacca cactacaggc tgatccaact ttgatgtaat gtgcttaaaa ctagtcaaaa 11281 tgaggctcag tattgtgtgt ggcctccacg tgcctgtatg acctccctac aacgcctggg 11341 catgctcctg atgaggtggc ggatggtctc ccgagggatc tcctcccaga cctggactaa 11401 agcatccgcc aactcctgga cagtctgtgg tacaacgtgg cgttggtgga tggagcgaga 11461 catgatgtcc cagatgtgct caattggatt caggtctggg gaacgggcgg gccagtccat 11521 agcatcaatg ccttcctctt gcagaaactg ctgacacact ccagccacat gaggcctagc 11581 attgtcttgc attaggagga acccagggcc aaccacacca gcatatggtc tcacaagggg 11641 tctgaggatc tcatctcggt acctaatggc agtcaggcta cctctggcaa gcacatggag 11701 ggctgtgcgg ccccccaaag aaatgccacc acacaccatg actgacccac cgccaaaccg 11761 gtcatgctgg atgatgttgc aggcagaaga actttctcca cggcgtctcc agactgtcac 11821 gtctgtcaca tgtgctcagt gtgaacctgc tttcatctgt gaagagcaca gggcgccagt 11881 ggcgaatttg ccaatcttgg tgttctctgg caaatgccaa acgtcctgca cggtgttggg 11941 ctgtaagcac aacccccacc tgtggacgtc gggccctcat accaccctca tggagtctgt 12001 ttctgaccgt ttgagcagac acatgcacat ttgtggcctg ctggaggtca ttttgcaggg 12061 ctctggcagt gctcctcctg ctcctacttg cacaaaggcg gaggtagcgg tcctgctgct 12121 gggttgttgc cctcctacgg cctcctccac gtctcctgat gtactggcct gtctcctggt 12181 agcgcctcca tgatctggac actacgctga cagacacagc aaaccttctt gccacatctc 12241 gattgatgtg cgatcctgga tgagctgcac tacctgagcc acttgtgtgg gttgtagact 12301 ccatctcatg ctaccactag agtgaaagca ccgccagcat tcaaaagtga ccaaaacatc 12361 agccaggaag cataggaact gagaagtggt ctgtggtcac cacctgcaga accactcctt 12421 tattgggggt gtcttgctaa atgcctataa tttccacctg ttgtctattc catttgcaca 12481 acagcatgtg aaatttattg tcaatcagtg ttgcttctta agtggacagt ttgatttcac 12541 agaagtgtga ttgacttgga gttacattgt gttgtttaag tgttcccttt tttttgagca 12601 gtatatatat atatatatat atagctcccg agtggcgcgg tggtctaagg cactgcatct 12661 cagtgcaaga ggcgtcacta cagtccctgg ttcgaattca ggctgtatca catctggtcg 12721 ggattgggag tcccatagga aagcacacaa ttgtccgagt ttgaccgggg taggccatca 12781 ttgcaaataa gaatttgttc ttaactgact tgcctagtga aataaaagtt acatttgact 12841 tttttaaatt aaaatatccc tgcatacatt tgagtgtaca ggttgaggcc tccccattat 12901 ttctccatac attaatgtat ctatttcata actatacatt atctagcctc tcaaatactg 12961 tatattcatc aatattcccc agatgatttc ccaaaattag cacctgccta agtaagattg 13021 ttggatattt cttggataaa cgtaatcaga caagagttct caacaataca ttaatttatt 13081 ttcgtctctc cattttagtt ctctctccac acctgtcttc tctttctctt aatctctaca 13141 tctccattct ccttctcctc caccacccct ctttttacct gttgattatt cctttaattt 13201 gggagtcttt ctccacctgc tgttgccgta gcctcctctc gctgtcctcc aggcgactct