Anim. Syst. Evol. Divers. Vol. 28, No. 4: 312-316, October 2012 http://dx.doi.org/10.5635/ASED.2012.28.4.312 Short communication First Record of a Hyalid Species, Protohyale pumila (Crustacea: Amphipoda: Hyalidae), from Korea Myung-Hwa Shin, Won Kim* School of Biological Sciences, Seoul National University, Seoul 151-747, Korea ABSTRACT A hyalid amphipod, Protohyale pumila(Hiwatari and Kajihara, 1981), collected from Jeju Island is reported for the first time in the Korean fauna. The species is characterized by the presence of a short apical spine on the palp article 4 of maxilliped, a distinctly large and blunt cusp on the propodus of male gnathopod 1, and a distal truncate process on the palm of male gnathopod 2. Herein, the present species is described and illustrated. Korean hyalid amphipods now are composed of four species belonging to three genera. Voucher specimens were deposited in the Marine Arthropod Depository Bank of Korea, Seoul National University. Keywords:Protohyale pumila, Hyalidae, Amphipoda, marine, Korea INTRODUCTION rostrum to the posterior end of urosomite 3. All specimens examined herein were deposited in the Marine Arthropod Members of the family Hyalidae are part of the littoral marine Depository Bankof Korea(MADBK,http://madbk.org)locat- amphipod group and mainly inhabit intertidal zones and ed at Seoul National University, Korea. shallow water. Most members of the group are found among algae living in warmer waters(Barnard and Karaman, 1991; Hiwatari, 2003). A few subgroups of the hyalid amphipods, SYSTEMATIC ACCOUNTS including the genus Protohyale Bousfield and Hendrycks, 2002, are known for having a simple-form preamplexing Order Amphipoda Latreille, 1816 notch of peraeon segment 2 in female. The dactylus of male Family Hyalidae Bulycheva, 1957 gnathopod 1 meshes correctly with and is used to grasp the Genus ProtohyaleBousfield and Hendrycks, 2002 precopularoty notch of the female(Hendrycks and Bousfield, 2001). Key to the species of the genus Protohyalefrom Korea Only one species, P. rubra(Thomson, 1879), has been 1. Propodus of gnathopod 1 in male and female dissimilar found in Korea until now. In the present study, Protohyale to each other; palm of male gnathopod 2 with distinct pumila(Hiwatari and Kajihara, 1981) is reported for the first truncate process ∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙ time as a member of the Korean amphipod fauna. ∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙P. pumila(Hiwatari and Kajihara, 1981) Specimens were collected among algae living in the inter- -Propodus of gnathopod 1 in male and female similar to tidal zone at Jeju Island, Korea and preserved in 95% ethanol. each other; palm of male gnathopod 2 without process ∙∙∙ A stereomicroscope(MZ8; Leica, Wetzlar, Germany) and a ∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙∙P. rubra(Thomson, 1879) compound microscope(BX-50; Olympus, Tokyo, Japan) were used for the observation. Dissection and mounting were 1*Protohyale pumila(Hiwatari and Kajihara, 1981) conducted following the methodology of Barnard and Kara- (Figs. 1--3) man(1991). Illustrations were made by using a drawing tube Hyale dollfusi: Iwasa, 1939: 280, Pl. 18, text-fig. 18; Steph- or camera lucida. Body length was measured from the tip of ensen, 1944: 69, fig. 24 [not Chevreux, 1911]. Korean name: 1*꼬마채찍해조숨이옆새우 cc This is an Open Access article distributed under the terms of the Creative *To whom correspondence should be addressed Commons Attribution Non-Commercial License(http://creativecommons.org/ Tel: 82-2-880-6695, Fax: 82-2-872-1993 licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, E-mail: [email protected] and reproduction in any medium, provided the original work is properly cited. pISSN 2234-6953 eISSN 2234-8190 Copyright The Korean Society of Systematic Zoology Protohyale pumilafrom Korea Fig. 1.Protohyale pumila(Hiwatari and Kajihara), male. Body. Scale bar==1mm. Hyale pumilaHiwatari and Kajihara, 1981: 35, figs. 1-4. Coxa of pereopod 2(Figs. 2C, 3A) widest in middle, with Protohyale(Boreohyale) pumila: Bousfield and Hendrycks, posteroproximal excavation; propodus bearing pair of lock- 2002: 77, fig. 36. ing spines, locking spines subequal in size, heavily striated. Basis of pereopods 3-5(Figs. 2D-F, 3B) broad, subcir- Material examined. Korea: 216��, 21♀♀, Jeju-do, Jo- cular, weakly serrate on posterior margin; merus and carpus cheon-eup, Hamdeog-ri, Hamdeog Beach, 29 May 2007. slender; propodus having pair of locking spines, proximal Description.Male:Body(Fig. 1) 6.98mm long, compressed. locking spine larger than distal one, locking spines weakly Lateral cephalic lobe slightly convex, anteroventral cephalic striated. excavation shallow. Eye black, large. Terminal part of anten- Peduncle of uropod 1(Fig. 3C) as long as rami, having na 1 reaching about one-fourth portion of antenna 2 flagel- large distolateral spine and marginal spines; inner and outer lum. Antenna 2 about 0.5 times as long as body length, pe- rami with three dorsal spines, respectively. duncle 5 slightly longer than peduncle 4. Palp article 4 of Peduncle of uropod 2(Fig. 3D) almost as long as rami, maxilliped slightly broad, bearing short apical spine and having marginal spines; inner and outer rami each with two short marginal setae. dorsal spines. Coxa of gnathopod 1(Fig. 2A) broad; basis without ante- Peduncle of uropod 3(Fig. 3E) about 1.5 times as long as rodistal lobe; carpus broad, bearing five spines on anterodis- ramus, bearing five inner marginal setae and two apicodor- tal margin, with expanded posterodistal lobe; propodus broad- sal spines; ramus with six apical spines. ly expanded, anterior margin unarmed, with posterior mar- Telson(Fig. 3F) completely cleft, both lobes having four ginal setae; palm convex, defined by large and blunt cusp, small setae on lateral margin. with one spine near defining corner; dactylus fitting palm. Female: Body 6.12mm long, slightly smaller than that of Coxa of gnathopod 2(Fig. 2B) subquadrate; basis bearing male. anterodistal lobe; ischium with subcircular lobe; merus slight- Basis of gnathopod 1(Fig. 3G) weakly swollen anterodis- ly extended; propodus very large, anterior margin convex; tally; propodus slender, subrectangular, middle part of pos- palm oblique, subequal as long as posterior margin, with dis- terior margin concave; palm oblique, simple; dactylus fitting tal truncate process having one spine and several setae, whole palm. of palm bearing spines; dactylus fitting palm. Gnathopod 2(Fig. 3H) similar to gnathopod 1, rather lar- Anim. Syst. Evol. Divers. 28(4), 312-316 313 Myung-Hwa Shin, Won Kim A B F D E C Fig. 2.Protohyale pumila(Hiwatari and Kajihara), male. A, Gnathopod 1; B, Gnathopod 2; C, Pereopod 2; D, Pereopod 3; E, Pereopod 4; F, Pereopod 5. Scale bars: A-F==0.5mm. 314 Anim. Syst. Evol. Divers. 28(4), 312-316 Protohyale pumilafrom Korea E F D C B I A G H Fig. 3.Protohyale pumila(Hiwatari and Kajihara), male. A, Locking spines of pereopod 2 propodus; B, Locking spines of pereopod 5 propodus; C, Uropod 1; D, Uropod 2; E, Uropod 3; F, Telson. Female. G, Gnathopod 1; H, Gnathopod 2; I, Preamplexing notch of peraeon 2. Scale bars: A, B==0.05mm, C, D, G, H==0.3mm, E, F==0.1mm, I==0.2mm. Anim. Syst. Evol. Divers. 28(4), 312-316 315 Myung-Hwa Shin, Won Kim ger. ACKNOWLEDGMENTS Pereon segment 2(Fig. 3I) bearing split-like preamplexing notch; posterior lobe strongly expanded forward, and over- The authors thank Prof. Wim Vader(Tromsø Museum, Uni- lapping with anterior lobe; without locking slit on surface. versity of Tromsø, Norway) for helpful comments. This study Distribution.Korea(Jeju Island), Japan. was supported by a grant from the Marine Biotechnology Remarks.Protohyale pumila(Hiwatari and Kajihara, 1981) Programme, funded by Ministry of Land, Transport and is similar to P. laie(Barnard, 1970) and Hyale latimanaHi- Maritime Affairs of the Korean Government. watari, 2003. Each of the three species has a defining large and blunt cusp on the palm of male gnathopod 1, and one spine on the defining corner. However, the case of P. laie REFERENCES and H. latimana, the anterodistal margin of the propodus of male gnathopod 1 is tumid in shape, whereas that of P. pu- Barnard JL, Karaman GS, 1991. The families and genera of milais not. In P. laieand H. latimana, there is no process on marine gammaridean Amphipoda(except marine gamma- the palm of the male gnathopod 2, whereas there is a distinct roids). Records of the Australian Museum, Supplement, 13: truncate process in P. pumila. In P. laie, the palm of male 1-866. Bousfield EL, Hendrycks EA, 2002. The talitroidean amphipod gnathopod 2 is much longer than the posterior margin, where- family Hyalidae revised, with emphasis on the North Pacific as that of each of P. pumila and H. latimana is almost as fauna: systematics and distributional ecology. Amphipaci- long as the margin. fica, 3:17-134. The characteristics of our specimens coincide with those Chevreux E, 1911. Campagnes de la Melita. Les amphipodes shown in Hiwatari and Kajihara’s original description based d’algerie et de tunisie. I. gammarina. Mémoires de la Société on specimens from Japan. Minor differences were observed Zoologique de France, 23:143-285. in the shape of the carpus of gnathopod 1 and the ischium of Hendrycks EA, Bousfield EL, 2001. The amphipod genus Allor- gnathopod 2. The shape of male specimens was more round- chestes in the North Pacific region: systematics and distri- ed compared to that of the original description. butional ecology. Amphipacifica, 3:3-37. Recently, the large genus Hyalein the family Hyalidae was Hiwatari T, 2003. Taxonomic studies on Hyale(Crustacea, Am- revised by Bousfield and Hendrycks(2002) based on North phipoda, Hyalidae) from the coast of Japan and adjacent Pacific fauna. They proposed five new genera from the genus waters. Publication of the Seto Marine Biological Labora- tory, 39:229-262. Hyale. Now, six genera, Hyale Rathke, 1837, Apohyale Hiwatari T, Kajihara T, 1981. Taxonomy of the family Hyalidae Bousfield and Hendrycks, 2002, Protohyale Bousfield and (Amphipoda, Crustacea) in Japan. II. A new species of the Hendrycks, 2002, PtilohyaleBousfield and Hendrycks, 2002, genus Hyale. Proceedings of the Japanese Society of Sys- RuffohyaleBousfield and Hendrycks, 2002, and Serejohyale tematic Zoology, 21:35-40. Bousfield and Hendrycks, 2002, are valid(Serejo, 2004). Of Hughes LE, Lowry JK, 2006. New species of Amphipoda(Cru- these, the genus Protohyale was split into four subgenera: stacea: Peracarida) from the Solitary Islands, New South Boreohyale, Diplohyale, Leptohyale and Protohyale. In the Wales, Australia. Zootaxa, 1222:1-52. revision by Bousfield and Hendrycks(2002), Hyale pumila Iwasa M, 1939. Japanese Talitridae. Journal of the Faculty of was transferred as Protohyale(Boreohyale) pumila. However, Science Hokkaido Imperial University, Series VI, Zoology, Hughes and Lowry(2006) did not follow the subgeneric status 6:255-296. of Bousfield and Hendrycks(2002), because the subgeneric Serejo CS, 2004. Cladistic revision of talitroidean amphipods (Crustacea, Gammaridea), with a proposal of a new classifi- taxonomy of Protohyale does not completely cover the cation. Zoologica Scripta, 33:551-586. Australian species. Therefore, they combined four subgenera Stephensen K, 1944. Some Japanese amphipods. Videnskabelige into the genus Protohyale. In this study, we placed our spe- Meddelelser fra Dansk Naturhistorisk Forening, 108:25-88. cies pumila in the genus Protohyale following the scheme of Hughes and Lowry. Received July 17, 2012 Revised September 4, 2012 Accepted September 9, 2012 316 Anim. Syst. Evol. Divers. 28(4), 312-316