Feasibility Analysis of Translocating Western Pearlshell Mussels into the Cherry Creek Native Trout Project Reach on the Flying D Ranch of Montana A Report to Turner Enterprises, Inc. by David Stagliano Montana Natural Heritage Program Helena, Montana April 2013 a program of the Montana State Library's Natural Resource Information System operated by the University of Montana Introduction The western pearlshell mussel (WEPE), Margaritifera falcata has experienced significant range reductions across Montana in the last 100 years and is now known from ~88 populations, of which, only ~20 are expected to be viable 100 years from now (Stagliano 2010). Many of these remaining populations are also at risk of extirpation due to stochastic events capable of wiping out these isolated small populations, and from lack of reproduction with non-native salmonid host species. Cherry Creek below the natural canyon barrier on the Flying D Ranch is one such stream that currently has a non-viable population (D-rank, Stagliano unpublished data 2010), and closer to the confluence with the Madison River, mussels remain sparse and non-viable (Stagliano 2008, unpublished data). The declining status of the WEPE has led to its designation as a Tier 1 Species of Conservation Need (SGCN) by MT Fish Wildlife and Parks (2006), a Species of Concern by the State of Montana (MTNHP 2008) and a Sensitive Species by the U.S. Forest Service Northern Region 1 (2011, S. Spaulding pers. comm.). The best approach to conserving aquatic species is to ensure that habitats are managed in ways that maintain healthy ecosystems and allow the full complement of native species to flourish. Turner Enterprises has come a long way to achieve this goal on the Flying D Ranch with the restoration of Cherry Creek to a native westslope cutthroat trout stream. To fully succeed in the goal of harboring all native aquatic species, the western pearlshell mussel, found naturally below the natural barrier but prefers the westslope cutthroat trout as its native host fish, needs to be included in the Cherry Creek restoration plan. Objectives. This project will: 1) evaluate the feasibility of translocating the WEPE into Cherry Creek above the natural fish barrier on the Flying D Ranch by evaluating habitat suitability throughout the ~10 mile reach of Cherry Creek in the context of stream habitat parameters known to contain populations of WEPE in Montana, 2) Measure and identify stream geomorphology parameters known to be favored by WEPE including stable stream reaches with point bars, willow/alder dominated riparian, minimal bedload movement and a Rosgen C3-C4 stream classification (i.e. gradient <2%, >1.2 sinuosity, >12 width/depth ratio, avg. substrate size 32-45mm) (Rosgen 1996), 3) Identify a suitable donor population in the Madison River watershed and have 30 specimens from that population tested by the FWP Fish Health Committee (following MTFWP 2010 protocols). If all objectives are met, we would be positioned to begin translocations in 2013 given positive habitat feasibility ranks and a certified disease-free, donor population. Methods. We used widely accepted Rosgen Level II methodology to evaluate, quantitatively measure and analyze stream geomorphology on Flying D Ranch Cherry Creek sections (Rosgen 1996). Parameters such as channel gradient and stability, point bar formation and sinuosity were measured by walking the reach at the high water mark and stretching a chain-man line as a distance measure, while a straight line measure was calculated from GPS readings taken at the top and bottom of the reach using a Garmin 60 CSX; the ratio of chain-man distance to straight line measures is the calculated sinuosity. Gradient was calculated with the laser level and stadium rod as the difference between stadium height reading at the wetted edge of the laser location minus the stage height reading at a distance of at least 20x wetted widths downstream divided by that reach distance. Channel cross sectional data was measured using a laser level at the crest of the riffles where the pebble count (100 particles counted) was performed. This data was entered on standardized Laser Level Channel Cross-Section, Pebble Count and Stream Classification forms to provide the necessary variables to assign a Rosgen Stream Classification. We then ranked suitability for WEPE translocation based on the stream classification, with reaches classified as C4 ranking highest. We also searched database records for previous mussel surveys in the Madison River basin and performed additional mussel surveys on streams that had few to no survey records in the basin. We used visual encounter surveys standardized for the statewide mussel surveys protocols (Stagliano 2010). Results We performed Rosgen Level II evaluations on ten Cherry Creek stream reach sections, seven above the natural barrier in the Butler Reach and three above the man-made barrier (Table 1, Map 1 and 2). Stream reach sites behind and upstream of the ranch house (sites #825-828) and just upstream of the natural barrier canyon (site #832) have limited potential as WEPE translocation sites due to the higher gradient and larger substrate classes (Rosgen B3) as indicated in their pebble counts (Table 1, Appendix A). The most suitable WEPE translocation sites are within the Butler Reach (4 sites) and one reach above the man-made barrier (#823) (Table 2, Map 1 & 2). Table 1. Rosgen II field measurements used in stream classification. Highest ranking preferred WEPE habitat site are bolded and shaded. Bankfull Riffle Entrench Width / Stream Rosgen Site Channel Estimated Pebble Suitability ment Depth Slope Stream ID Width Sinuosity Count Rank Ratio Ratio (%) Type (m) D50 116 6.7 2.1 24.0 1.20 1.9 90-128 B3/C3b 119 6.9 2.8 25.4 1.67 0.9 45-64 C4b 5th 819 7.0 2.2 26.0 1.50 1.1 64-90 C3b 5th 821 9.7 2.5 31.5 1.90 0.6 64-90 C3 4th 823 11.4 2.3 34.0 1.14 0.5 32-45 C4 3rd 825 6.6 2.2 20.1 1.20 1.7 90-128 B3/C3b 826 na na na na na na na 827 10.1 8.0 32.2 1.20 1.5 90-128 C3 829 8.8 2.1 22.0 1.20 1.8 90-128 B3/C3b 830 10.1 4.1 18.7 1.35 0.8 64-90 C4/C3 2nd 831 10.1 4.1 18.7 1.35 0.8 64-90 C4/C3 2nd 832 9.5 1.8 14.0 1.10 2.1 90-128 B3 833 10.2 2.9 29.0 1.24 0.7 45-64 C4 1st 834 10.2 2.9 29.0 1.24 0.7 45-64 C4 1st Table 2. Cherry Creek and Spanish Creek sites visited during the TEI Ranch Surveys 2012. Stream sites are organized from higher elevation --> downstream. Shaded sites are those ranked most suitable for WEPE translocations. TDS = Total Dissolved Solids Site Elevation Rosgen Temp Cond Site Description Photo Link Number Site Code latitude longitude Date/time (m) Class °C (µs/sec) pH TDS Cherry Creek above the ranch reach, boulders with http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 829 UM_CC829 45.5157 -111.4478 7/24/2012 7:37 1742 B3/C3 some embedded cobbles trapping sand 12.1 37 7.8 18 MusselSurveys/UM_CC829.JPG Cherry Creek above the active beaver pond, B3/C3 http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 827 UM_CC827 45.5188 -111.4453 7/24/2012 7:21 1735 C3 Rosgen reach boulder dominated 12.2 39 7.7 19 MusselSurveys/UM_CC827.JPG Cherry Creek behind the cow camp with an active http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 826 UM_CC826 45.5222 -111.4478 7/24/2012 7:12 1731 na beaver pond 12.7 38 7.7 20 MusselSurveys/UM_CC826_bp.JPG http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 825 UM_CC825 45.5226 -111.4459 7/24/2012 7:09 1706 B3/C3b Cherry Creek behind the cow camp 12.3 37 7.7 19 MusselSurveys/UM_CC825.JPG http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 823 UM_CC823 45.5508 -111.4466 7/23/2012 19:30 1674 C4 Cherry Creek above the man-made barrier 11.7 126 8.1 62 MusselSurveys/UM_CC823_ov.JPG Cherry Creek upstream of site 819 at upper end of http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 116 UM_CC116 45.5512 -111.4460 7/23/2012 14:02:001 P6M72 B3/C3b Bulter Reach 12.5 122 8.1 60 MusselSurveys/UM_CC116.JPG Cherry Creek on the Turner Ranch upper end of http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 819 UM_CC819 45.5542 -111.4443 7/23/2012 12:54 1671 C3b Butler Reach 12.5 124 8.1 62 MusselSurveys/UM_CC821_t.JPG Cherry Creek on the Turner Ranch upper end of http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 821 UM_CC821 45.5579 -111.4446 7/23/2012 14:54 1670 C3 Butler Reach 12.9 129 8.2 62 MusselSurveys/UM_CC821.JPG Cherry Creek upstream of site 833 at lower end of http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 119 UM_CC119 45.5630 -111.44447/23/2012 13:54:581 P66M8 C3b Bulter Reach 11.7 124 8.1 62 MusselSurveys/UM_CC119.JPG Cherry Creek in the Butler meadow reach http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 833 UM_CC833 45.5652 -111.4419 7/24/2012 10:53 1665 C3/C4 transistioning from a C3 to a C4 12.2 119 8.1 62 MusselSurveys/UM_CC833.JPG Cherry Creek in the Butler meadow reach, great C4 http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 834 UM_CC834 45.5642 -111.4417 7/24/2012 11:06 1664 C4 WEPE habitat 12.2 119 8.1 62 MusselSurveys/UM_CC833_u.JPG Cherry Creek in the lower butler meadow reach http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 830 UM_CC830 45.5659 -111.4405 7/24/2012 9:22 1663 C3/C4 transistioning from a C3 to a B3 11.5 115 8.2 57 MusselSurveys/UM_CC830.JPG Cherry Creek in the lower butler meadow reach http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 831 UM_CC831 45.5654 -111.4409 7/24/2012 9:28 1662 C3/C4 transistioning from a C3 to a B3 11.5 115 8.2 57 MusselSurveys/UM_CC831.JPG Cherry Creek exiting the Butler reach transistioning http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 832 UM_CC832 45.5674 -111.4391 7/24/2012 10:31 1660 B3 from a C3 to a B3 12.2 119 8.1 62 MusselSurveys/UM_CC832.JPG Cherry Creek below the natural barrier transistioning http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 835 UM_CC835 45.5947 -111.4907 7/24/2012 12:13 1388 C3 from a C3 to a B3 15.6 141 8.5 70 MusselSurveys/UM_CC835.JPG Cherry Creek below the natural barrier transistioning http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 836 UM_CC836 45.5938 -111.4890 7/24/2012 12:33 1386 B3 from a C3 to a B3 15.6 141 8.5 70 MusselSurveys/UM_CC836_d.JPG http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 816 UM_CC816 45.6172 -111.5432 7/23/2012 10:13 1363 C4 Cherry Creek below the county road bridge off Ranch 17.8 165 8.0 82 MusselSurveys/UM_CC816.JPG http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 837 UM_SPC837 45.4930 -111.3676 7/24/2012 14:10 1722 B3 Spanish Creek on the TEI Property 16.3 43 7.9 22 MusselSurveys/UM_SPC837.JPG http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 838 UM_SPC838 45.4986 -111.3240 7/24/2012 14:30 1668 C3 Spanish Creek on the TEI Property 16.5 44 7.9 24 MusselSurveys/UM_SPC838.JPG http://mtnhp.org/ThumbsPlus/Eco/Aquatics/ 839 UM_SPC839 45.4976 -111.3251 7/24/2012 14:43 1665 C3 Spanish Creek on the TEI Property 16.5 44 7.9 22 MusselSurveys/UM_SPC839.JPG Map 1. Cherry Creek on TEI Property from manmade barrier (site 115) downstream into Butler Reach 5th choice Relocation 3rd choice Relocation No Relocation B3 Channel Map 2. Cherry Creek continued from Map 1 (site 820) downstream thru Butler Reach to the canyon (Site 832) No Relocation Option entering Canyon 2nd choice Relocatation 4th choice Relocate 1st choice Relocation We surveyed 25 previously unvisited stream reaches in 11 streams within the Madison and Jefferson River Basins during the summer of 2012 trying to locate a donor WEPE population (Map 3). Despite considerable survey effort, we did not find a single live WEPE mussel. We identified recently dead WEPE shells in a new reach of O’Dell Spring Creek indicating the Map 3. Western Pearlshell surveys performed in 2012 potential of a live population (D-rank viability), but the two other streams containing WEPE shells, the Ruby and Jefferson Rivers, only had relic shells (>10 years old)and are not likely to contain live populations. Of fifty-one stream reaches in the Madison River basin that had been surveyed for WEPE prior to 2012 (Appendix B), only one of those reaches was ranked as having a viable WEPE population (Duck Creek, B-rank)(Map 4). We did not return to Duck Creek in 2012 to collect mussels for the fish health screening, but plan to revisit this site in 2013 to Map 4. Madison Basin WEPE surveys performed prior to 2012 evaluate Whirling Disease Status with a backpack shocking grab sample of rainbow trout and a WEPE collection to submit for health screen analysis as required for translocation (FWP 2010). Previous whirling disease testing on 136 total trout from Duck Creek in 2004, 2005 and 2007 showed 0% infected, except for 1 outlier in 2007 (1 out of 43 infected at a Grade 5)(P. Clancy, pers. comm.). Pat said “When they got a real outlier like this they attributed it to a fish getting mixed into the wrong aquarium at Pony (they had occasional problems with jumpers), a mix up in processing prior to sending to the lab, or a mix-up at the lab.” Thus, Duck Creek could be considered WD- and our Madison River basin donor WEPE population with an additional WD negative trout sample and a clean Fish Health Mussel Screen in 2013. Conclusion Completion of extensive habitat analysis within the Cherry Creek Native Trout Project Reach, allows us to have the scientific evidence along with our professional opinion to confidently determine that at least five reaches of Cherry Creek on the Turner Flying D Ranch contain the preferred stream habitat, as well as suitable densities of their native host fish (WSCT) capable for allowing the western pearlshell mussel to survive, reproduce, and persist once translocated to this stream. As evidenced by our 2012 and previous surveys, WEPE populations are in decline throughout the Madison River basin and another secure (viable) population in Cherry Creek would considerably aid conservation efforts for this species. Literature Cited Montana Fish, Wildlife and Parks 2010. Environmental Assessement: Western pearlshell mussel translocation project, Blackfoot River Basin, MT. Preparers: R. Pierce, L. Knotek and D. Stagliano. http://fwpiis.mt.gov/content/getItem.aspx?id=43287 Rosgen, D. 1996. Applied river morphology manual. Wildlands Hydrology, Pagosa Springs, CO Stagliano, D.M. and R.G. Pierce 2011. Western Pearlshell Mussel Translocation Project in the Blackfoot River Watershed: How not to treat mussels prior to relocation. Presented at the 44th Annual MT Chapter AFS Meeting, Great Falls, MT Stagliano, D. 2010. Freshwater Mussels in Montana: Comprehensive Results from 3 years of SWG Funded Surveys. Report to MT Fish Wildlife and Parks, Helena, MT 41 pp. + appendices