Endocytosis From Cell Biology to Health, Disease and Therapy NATO ASI Series Advanced Science Institutes Series A series presenting the results of activities sponsored by the NATO Science Committee, which aims at the dissemination of advanced scientific and technological know/edge, with a view to strengthening links between scientific communities. The Series is published by an international board of publishers in conjunction with the NATO Scientific Affairs Division A Life Sciences Plenum Publishing Corporation B Physics London and New York C Mathematical and Kluwer Academic Publishers Physical Sciences Dordrecht, Boston and London D Behavioural and Social Sciences E Applied Sciences F Computer and Springer-Verlag Systems Sciences Berlin Heidelberg New York G Ecological Sciences London Paris Tokyo Hong Kong H Cell Biology Barcelona Budapest I Global Environmental Change NATo-peo DATABASE The electronic index to the NATO ASI Series provides full bibliographical references (with keywords and/or abstracts) to more than 30000 contributions from international scientists published in all sections of the NATO ASI Series. Access to the NATO-PCO DATABASE compiled by the NATO Publication Coordination Office is possible in two ways: - via online FILE 128 (NATO-PCO DATABASE) hosted by ESRIN, Via Galileo Galilei, 1-00044 Frascati, Italy. - via CD-ROM "NATO-PCO DATABASE" with user-friendly retrieval software in English, French and German (© WTV GmbH and DATAWARE Technologies Inc. 1989). The CD-ROM can be ordered through any member of the Board of Publishers or through NATO-PCO, Overijse, Belgium. Series H: Cell Biology Vol. 62 Endocytosis From Cell Biology to Health, Disease and Therapy Edited by Pierre J. Courtoy Cell Biology Unit Louvain University Medical School and Intemationallnstitute of Cellular and Molecular Pathology UCL 75.41 75. avo Hippocrate 1200 Brussels Belgium With the assistance of Alice Dautry-Varsat Jean Gruenberg Lucinda Mata Mark Marsh Antoinette Ryter 80 van Deurs Springer-Verlag Berlin Heidelberg New York London Paris Tokyo Hong Kong Barcelona Budapest Published in cooperation with NATO Scientific Affairs Division Proceedings of the NATO Advanced Research Workshop on Endocytosis, held at Paris, France, from October 1-5, 1990 Additional material to this book can be downloaded from http://extra.springer.com. ISBN-13: 978-3-642-84297-9 e-ISBN-13: 978-3-642-84295-5 DO I: 10.1007/978-3-642-84295-5 This work is subject to copyright. All rights are reserved, whether the whole or part ot the material is concemed, specifically the rights of translation, reprinting, re-use of illustrations, recitation, broadcasting, reproduction on microfilms or in any other way, and storage in data banks. Duplication of this publication or parts thereof is per mitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permisSion for use must always be obtained from Springer-Verlag. Violations are liable for prosecution under the German Copyright Law. © Springer-Verlag Berlin Heidelberg 1992 Softcover reprint of the hardcover 1s t edition 1992 Typesetting: Camera-ready by authors 31/3145-543210 - Printed on acid-free paper CONTENTS v Contents Foreword XIII I. STRUCTURE, DYNAMICS AND FUNCTIONS OF THE ENDOCYTIC APPARATUS 1.1. Surface events. Mobility .. and clustering 1 . Dynamic cytomatrix-membrane interactions investigated with Nanovid microscopy M. De Brabander, R. Nuydens and H. Geerts 3 Analysis of receptor dynamics, as studied with surface replicas H. Robenek 9 Surface distribution and pathway of internalization of C3b receptors (CRt) in human neutrophils J.-P. Paccaud and J.-L. Carpentier 17 1.2. Clathrin-coated pits and clathrin-independent endocytosis 25 The dynamics of clathrin coats in living cells measured by advanced fluorescence microscopy J. Davoust and P. Cosson 27 Assembly proteins and adaptors in clathrin-coated vesicles E. UngewickeU, R. Linder and S. Schroder 43 Molecular biology of the adaptins M.S. Robinson 51 Clathrin-independent endocytosis B. van Deurs, SR. Hansen and K. Sandvig 57 1.3. Dynamics of endosomes 63 New insights into the endocytic pathway C.R. Hopkins and K.M. Miller 65 The compartments of the endocytic pathway G. Griffiths 73 VI Protein exchange within the lysosome and the pre-lysosome compartment. A mechanism for maintaining organelle functionality ? B. Storrie and Y. Deng 85 Endosomal pH regulation and the maturation model for lysosome biogenesis R.F. Murphy, M. Roederer, D.M. Sipe, e.C. Cain and R.B. Wilson 91 1.4. Cell-free analysis of endocytic traffic 97 . Effect of AT PyS on the formation of coated vesicles broken 10 HeLa cells M. Pypaert and G. Warren 99 A TP is required for receptor-mediated endocytosis both in vivo and in vitro S.L. Schmid, L.L. Carter and E. Smythe 105 Regulation of endocytic membrane traffic J. Gruenberg, J.-P. Gorvel and M. Bomsel 113 The interaction of late endosomes with lysosomes in a cell-free system J.P. Luzio and B.M. Mullock 123 Roundtable. Cell-free systems K. Howell 131 1.5. Molecular processing by endosomes 133 Regulation of AT P-dependent endosome acidification R. Fuchs, S. Schmid, I. Mellman and H. Klapper 135 Degradation of insulin and glucagon in isolated liver endosomes. Functional relationships with ATP-dependent endosomal acidification and partial characterization of degradation products. B. Desbuquois, F. Authier, J.P. Clot, M. Janicot and F. Fouque 141 Signaling of receptor tyrosine kinases across the endosomal membrane J.J.M. Bergeron and B.I. Posner 151 VII 1.6. Recycling pathways 159 Membrane recycling in the epithelium of seminal vesicle intersects the secretory pathway L.R. Mata and E.l. Christensen 161 Endocytic pathways W. Stoorvogel, H.J. Geuze and G.J. Strous 169 Intracellular traffic of the growth factor interleukin-2 and its receptors in human T lymphocytes M. Ferrer, A. Hemar, V. Duprez and A. Dautry-Varsat 175 Two recycling pathways of epidermal growth factor-receptor complexes in A431 cells A. Sorkin, E. Korni/ova and S. Krolenko 181 Oligosaccharide reprocessing of plasma membrane glycoproteins R. Tauber, W. Kreisel and W. Reutter 187 1.7. Biogenesis of lysosomes 195 Lysosomal enzyme transport between Golgi and endosomes T. Ludwig, S. Meresse and B. Hoflack 197 The nineteen amino acid-cytoplasmic tail of lysosomal acid phosphatase contains an endocytosis signal necessary and sufficient for targeting to lysosomes Ch. Peters, M. Braun, B. Weber, M. Wendland, B. Schmidt, R. Pohlmann, A. Waheed and K. von Figura 205 Biochemical characterization, structure and transport of lysosomal integral membrane proteins B. Segui-Real, M.A. Vega, J.G. Barriocanal, L. Yuan, J. Alcade and I. V. Sandoval 213 Mannose phosphate-independent membrane association of lysosomal enzymes occurs after passage of the Golgi complex G.J. Strous, H.J. Geuze, H.M.F.G. Aerts, J.M. Tager and S. Rijnboutt 221 VIII 1.8. Heterogeneity of lysosomes and autophagy 229 LGPlOD10, a lysosomal membrane protein S. Wattiaux-De Coninck, M.M. Gonze, L. De Waele, F. Mainjerme, P. Van Der Smissen, P.J. Courtoy, J. Thirion, J.J. Letesson and R. Wattiaux 231 Receptor-mediated endocytosis in liver endothelial cells. Evidence of lysosomal heterogeneity T. Berg, S. Magnusson, E. Stang and N. Roos 239 Autophagic-endocytic interactions in hepatocytes P.O. Seglen, P.B. Gordon, I. Holen, H. Hoyvik, AL. Kovacs, P.E. Stromhaug and T.O. Berg 247 1.9. The biogenesis of epithelial polarity 255 Plasma membrane biogenesis and vesicle traffic in polarized hepatocytes. What can perturbations tell us ? A.L. Hubbard and V.A. Barr 257 Plasma membrane polarity of rat hepatocytes in primary culture M. Maurice, A.M. Durand-Schneider, I.C. Bouanga and G. Feldmann 267 Sorting of endogenous proteins in intestinal epithelial cells L.A. Ginsel, J. Klumperman, J.M. Tager and 1.A. Fransen 273 Lipid sorting by the formation of microdomains of sphingolipids. A model and its consequences G. van Meer, W. van 't Hoi, I. van Genderen and A. Sandra 281 1.10. Transcytosis 289 Analytical subcellular fractionation of receptor-mediated transcytosis in rat hepatocytes P.J. Courtoy, M. Leruth-Deridder, J.P. Vaerman and P. Baudhuin 291 Temperature dependence of transcytotic pathways in rat liver H. Klapper, J. Grai and R. Fuchs 301 Fc receptor-mediated endocytosis in polarized and non polarized cells I. Mellman, H. Miettinen, P. Male and W. Hunziker 309 IX . Endocytosis in MDCK cells R.G. Parton 317 Protein handling from apical and basolateral surfaces rat III and rabbit renal proximal tubules E.I. Christensen and S. Nielsen 325 II. ENDOCYTOSIS IN HEALTH, DISEASE AND THERAPY 2.1. Endocytosis in the immune response 333 Antigen processing in B lymphocytes C. Watts, P.A. Reid, M.A. West and H.W. Davidson 335 Endocytosis and biosynthetic transport of murine MHC class II molecules in antigen-presenting cells J. Davoust, P. Cosson, J.M. Escola, J. Henry, M. Humbert, G. Raposo and J. Salamero 341 Endocytosis. Molecular mechanism and role in the immune response F.M. Brodsky, L.E. Guagliardi, C.J. Galloway, B. Koppelman, S.L. Acton, D.H. Wong and I. Niithke 343 Endocytosis of CD4 in lymphoid and non-lymphoid cells A. Pelchen-Matthews, J.E. Armes and M. Marsh 355 Role of trans epithelial transport in triggering a mucosal immune response and in delivery of mucosal antibodies into secretions J.P. Kraehenbuhl, P. Michetti, R. Hirt, C. Perregaux, J. Mekalanos and M. Neutra 363 2.2. Phagocytosis and intracellular pathogens 367 Overview on the relations of pathogenic microorganisms with their host cell A. Ryter 369 Intracellular growth of Mycobacterium avium in macrophages. Consequences on membrane traffic and exchange of contents between endosomes, lysosomes and phagosomes C. de Chastellier, C. Frehel and T. Lang 375 x Morphology, composItIOn and functions of the endocytic compartment housing Leishmania amazonensis in infected macrophages J.C. Antoine and E. Prina 381 Entry into eukaryotic cells and intracellular movement of Shigella flexneri P.J. Sansonetti, N. High, T. Vasselon, P. Clerc, ML. Bernardini, A. Allaoui, H. d'Hauteville and J. Mounier 389 2.3. Entry of viruses and toxins 397 On the role of endocytosis in the entry of animal viruses M. Marsh and A. Pelchen-Matthews 399 Endocytic uptake of ricin and shiga toxin K. Sandvig, K. Prydz and B. van Deurs 405 Endocytosis of Clostridium spiroforme binary toxin by Hep2 cells P. Boquet and M.R. Popoff 413 2.4. Lysosomal diseases 425 Correlation between residual activity of a lysosomal hydrolase and the severity of the resulting -storage disease K. Sandhoff, E. Conzelmann, S. Michel and P. Leinekugel 427 Lysosomes as suicide bags R. Wattiaux, S. Wattiaux-De Coninck, M. Jadot, I. Hamer, V. Bielande and V. Beauloye 433 Lysosomal storage disorders and toxicity induced by amino glycoside antibiotics and polyanionic peptides B.K. Kishore, P. Maldague and P.M. Tulkens 439 2.5. Endocytosis in non-mammalian cells 447 Endocytosis in the emergence of eukaryotic life ~~D~ ~ Endocytosis in plants. Problems and perspectives D.G. Robinson, R. Hedrich, B. Herkt, W. Diekmann and M. Robert-Nicoud 459