AD Award Number: DAMDl7-01-2-0005 TITLE: Research and Operational Support for the Study of Militarily Relevant Infectious Diseases of Interest to the United States and Royal Thai Government PRINCIPAL INVESTIGATOR: MG Suebpong Sangkharomaya, M.D. COL Sorachai Nitayaphan, M.D., Ph.D. CONTRACTING ORGANIZATION: Armed Forces Research Institute of Medical Sciences Royal Thai Army Medical Component Bangkok 10400 Thailand REPORT DATE: January 2005 TYPE OF REPORT: Annual PREPARED FOR: U.S. Army Medical Research and Materiel Command Fort Detrick, Maryland 21702-5012 DISTRIBUTION STATEMENT: Approved for Public Release; Distribution Unlimited The views, opinions and/or findings contained in this report are those of the author(s) and should not be construed as an official Department of the Army position, policy or decision unless so designated by other documentation. 20050826 082 Form Approved REPORT DOCUMENTATION PAGE OMB No. 074-0188 Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining '*he data needed, and completing and reviewing this collection of information, Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Washington Headquarters Services, D rectorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302, and to the Office of Management and Budget, Paperwork Reduction Protect (0704-0188), Washington, DC 20503 1. AGENCY USE ONLY 2. REPORT DATE 3. REPORT TYPE AND DATES COVERED (Leave blank)I January 2005 Annual (1 Jan 2003 - 31 Dec 2004) 4. TITLE AND SUBTITLE 5. FUNDING NUMBERS Research and Operational Support for the Study of DAMD17-01-2-0005 Militarily Relevant Infectious Diseases of Interest to the United States and Royal Thai Government 6. AUTHOR(S) MG Suebpong Sangkharomaya, M.D. COL Sorachai Nitayaphan, M.D., Ph.D. 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) 8. PERFORMING ORGANIZATION Armed Forces Research Institute of REPORT NUMBER Medical Sciences Royal Thai Army Medical Component Banokok 10400 Thailand E-Mail: snitayap@mozart .inet. co. th 9. SPONSORING / MONITORING 10. SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) AGENCY REPORT NUMBER U.S. Army Medical Research and Materiel Command Fort Detrick, Maryland 21702-5012 11. SUPPLEMENTARY NOTES 12a. DISTRIBUTION / AVAILABILITY STATEMENT 12b. DISTRIBUTION CODE Approved for Public Release; Distribution Unlimited 13. ABSTRACT (Maximum 200 Words) Cooperative agreement # DAMD 17-01-2-0005 was implemented January 1, 2001 to provide funding support for Royal Thai Army at Armed Forces Research Institute of medical Sciences (AFRIMS) engaged in research activities in collaboration with US Army. Administrative, logistical and scientific personnel required to support the ongoing US Army AFRIMS research efforts, and utilities and maintenance required to support the US Army AFRIMS research effort. 14. SUBJECT TERMS 15. NUMBER OF PAGES 81 Not Provided 16. PRICE CODE 17. SECURITY CLASSIFICATION 18. SECURITY CLASSIFICATION 19. SECURITY CLASSIFICATION 20. LIMITATION OF ABSTRACT OF REPORT OF THIS PAGE OF ABSTRACT Unclassified Unclassified Unclassified Unlimited NSN 7540-01-280-5500 Standard Form 298 (Rev. 2-89) Prescribed by ANSI Std. Z39-18 298-102 FOREWORD Opinions, interpretations, conclusions and recommendations are those of the author and are not necessarily endorsed by the U.S. Army. V Where copyrighted material is quoted, permission has been obtained to use such material. __ Where material from documents designated for limited distribution is quoted, permission has been obtained to use the material. V_ Citations of commercial organizations and trade names in this report do not constitute an official Department of Army endorsement or approval of the products or services of these organizations. ,-7 In conducting research using animals, the investigator(s) adhered to the "Guide for the ___ Care and Use of Laboratory Animals," prepared by the Committee on Care and use of Laboratory Animals of the Institute of Laboratory Resources, National Research Council (NIH Publication No. 86-23, Revised 1985). __ For the protection of human subjects, the investigator(s) adhered to policies of applicable Federal Law 45 CFR 46. / In conducting research utilizing recombinant DNA technology, the investigator(s) adh red to current guidelines promulgated by the National Institutes of Health. In the conduct of research utilizing recombinant DNA, the investigator(s) adhered to the NIHjiuidelines for Research Involving Recombinant DNA Molecules. In the conduct of research involving hazardous organisms, the investigator(s) adhered to the CDC-NIH Guide for Biosafety in Microbiological and Biomedical Laboratories. PI - Signature Date MG Suebpong Sangkharomaya, MD. Principal Investigator Royal Thai Army, AFRIMS Bangkok 10400, Thailand TABLE OF CONTENTS FRONT COVER 1 SF 298 - REPORT DOCUMENTATION PAGE 2 FOREWORD 3 TABLE OF CONTENTS 4 1. INTRODUCTION 11 A. General 11 B. Statement of work 11 C. US ARMY AFRIMS research efforts at Department of Entomology 11 D. US ARMY AFRIMS research efforts at Department of Immunology 11 E. US ARMY AFRIMS research efforts at Department of Enteric Diseases 11 F. US ARMY AFRIMS research efforts at Department of Veterinary Medicine 12 G. US ARMY AFRIMS research efforts at Department of Virology 12 H. US ARMY AFRIMS research efforts at Department of Retrovirology 12 I. Space and Utilities Required 12 II. BODY 13 A. Department of Entomology, AFRIMS FY04 Research Accomplishments 13 1. Title of research project: Use of GIS to assess relative risk in Different biotopes within endemic scrub typhus areas 13 a. Investigators 13 b. Objectives 13 c. Methods 13 d. Results 13 e. Future plans 14 2. Title of research project: Use of a Geographic Information (GIS) to implement and evaluate the efficacy of targeted Vector control as a means of reducing malaria transmission 14 a. Investigators 14 b. Objectives 15 c. Methods 15 d. Results 16 e Future plans 18 3. Title of research project: Development of a chigger-challenge model for the evaluation of candidate scrub typhus vaccines 18 a. Investigators 18 b. Objectives 19 c. Methods 19 d. Results 20 e. Future plans 20 45. Title of research project: Production of Plasmodiium vivax sporozoites to support a human challenge model 20 a. Investigators 20 b. Objectives 21 c. Methods 21 d. Results 22 e. Future plans 23 B. Department of Immunology AFRIMS FY04 Research Accomplishments 23 1. Title of research project 23 a. Investigators 25 b. Objectives 25 c. Methods 25 d. Results 25 e. Future plans 30 C. Department of Enteric Diseases, AFRIMS FY04 Research Efforts 31 5 1. Title of research project: Surveillance of Antimicrobial Resistance of Enteric Pathogens in Indigenous Populations in Multiple Site within Thailand 31 a. Investigator 31 b. Objectives 31 c. Methods 31 d. Results 31 e. Future plans 31 2. Title of research project: Development and Standardization of Realtime PCR Assays for Detection and Characterization of Enteric Pathogens 31 a. Investigators 31 b. Objectives 32 c. Methods 32 d. Results 32 e. Future plans 32 3. Title of research project: Travelers' Diarrhea among US Forces Deployed for Operation Cobra Gold 32 a. Investigator 32 b. Objectives 32 c Methods 32 d Results 33 e. Future plans 33 4. Title of research project: Characterization of Enteric Pathogens 33 Isolated from Children in Phnom Penh a. Investigator 33 b. Objectives 33 c Methods 33 d Results 33 e. Future plans 33 D. Department of Veterinary Medicine AFRIMS FY04 Research Accomplishments 34 6 1. Title of research project: Antimalarial Drugs Efficacy Testing in the Rhesus Monkey (Macaca mulatta)/Plasmodium cynomolgi Relapsing Malaria Model 34 a. Investigators 34 b. Objectives 34 c. Methods 34 d. Results 34 e. Future plans 34 2. Title of research project: Care and Maintenance of Rhesus (Macaca mulatta) and Cynomolgus (Macaca fascicularis) Monkeys and Management of Breeding Colonies 34 a. Investigators 34 b. Objectives 34 c. Methods 34 d. Results 34 e. Future plans 34 3. Title of research project: Care and Maintenance of Laboratory Rodents and Rabbits, Maintenance of Rodent Breeding Colonies, and Quality Assurance/Quality Surveillance Program 36 a. Investigators 36 b. Objectives 36 c. Methods 36 d. Results 36 e. Future plans 36 4. Title of research project: A Plasmodium berghei-Mouse Model for Screening Antimalarial Drugs 36 a. Investigators 37 b. Objectives 37 c. Methods 37 e. Results 37 f. Future plans 37 7 5. Title of research project: Characterization and Validation of Anopheles dirus Sporozoite-Induced Mouse Malaria Models (ICR Mouse/Plasmodium berghei and P. Yoelli) 38 a. Investigators 38 b. Objectives 38 c. Methods 38 d. Results 38 e. Future plans 38 E. Department of Virology, AFRIMSFY04 Research Accomplishments 38 1. Title of research project: The Dengue Hemorrhagic Fever Project III: Continued Prospective Observational Studies of Children with Suspected Dengue 38 a. Investigators 39 1. Principal Investigators 39 2. Associate Investigators 39 b. Objectives 39 c. Study Specific Objectives 39 d. Methods 40 e. Results 41 f. Future plans 41 2. Title of research project: A Recombinant Hepatitis E Vaccine Efficacy Study In Nepalese Volunteers 42 a. Investigators 42 1. Principal Investigators 42 2. Associate Investigators 42 b. Objectives 42 c. Methods 42 d. Future plans 43 3. Title of research project: Prospective Study of Dengue Virus Transmission and Disease in Primary Schools and Villages In Kamphaeng Phet, Thailand 43 a. Investigators 43 b. Objectives 44 8 c. Study Specific Hypotheses 44 d. Methods 45 e. Results 46 f. Future plans 51 4. Title of research project: A Phase I/II Trial of a Tetravalent Live Attenuated Dengue Vaccine in Flavivirus Antibody NaYve Children 51 a. Background 51 b. Objectives 51 c. Methods 51 d. Principal Investigators 51 e. Results 52 f. Future Plans 52 5. Title of research project: A Phase 1/11 Trial of a Tetravalent Live Attenuated Dengue Vaccine in Flavivirus Antibody NaYve Infants 52 a. Background 53 b. Objectives 53 c. Methods 53 d. Principal Investigators 53 e. Results 54 f. Future Plans 54 6. Title of research project: Training and Workshops 54 a. Background: 54 b. Goals 55 c. Activities 55 7. Title of research project: Influenza Surveillance in Southeast Asia 55 a. Background 55 b. Goals 56 9 c. Methods 56 d. Progress 56 F. Department of Retrovirology, AFRIMS FY04 Research Accomplishments 56 1. Title of research project: Screening and evaluation of potential Volunteers for a preventive HIV-a vaccine trial in Thailand a. Investigators 56 b. Objectives 57 c. Methods 57 d. Results 57 e. Future plans 57 2. Title of research project: A Phase III Trial of Aventis Pasteur Live Recombinant ALVAC-HIV (vCP1521) Priming with VaxGen gpl20 B/E (AIDSVAX® B/E) Boosting in HIV- uninfected Thai Adults (RV144, HSRRB Log No. A-11048, BB-IND 8795) 57 a. Investigators 57 b. Objectives 57 c. Methods 58 d. Results 58 f. Future plans 58 Il. APPENDICES: 59 A. PERSONNEL ASSIGNED UNDER AGREEMENT 59 B. Publications 2004 65 C. Abstracts 2004 10