Artículo Article www.biotaxa.org/RSEA. ISSN 1851-7471 (online) Revista de la Sociedad Entomológica Argentina 77(3): 1-13, 2018 Diversity of Ceratopogonidae (Diptera: Culicomorpha) from the Reserva Natural Integral Punta Lara (Buenos Aires, Argentina) CAZORLA, Carla G.1*, MARINO, Pablo I.1, DÍAZ, Florentina2 & CAMPOS, Raúl E.3 1 División Entomología, Museo de La Plata, Paseo del Bosque s/n (B1900FWA) La Plata, Buenos Aires, Argentina, and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). *E-mail: [email protected] 2 Centro de Estudios Parasitológicos y de Vectores (CEPAVE), CONICET-UNLP. La Plata, Buenos Aires, Argentina. 3 Instituto de Limnología “Dr. Raúl A. Ringuelet”. CONICET-UNLP. La Plata, Buenos Aires, Argentina. Received 08 - V - 2018 | Accepted 21 - VIII - 2018 | Published 27 - IX - 2018 https://doi.org/10.25085/rsea.770301 Diversidad de Ceratopogonidae (Diptera: Culicomorpha) de la Reserva Natural Integral Punta Lara (Buenos Aires, Argentina) RESUMEN. Se presenta el primer relevamiento de Ceratopogonidae de la Reserva Natural Integral Punta Lara, en la provincia de Buenos Aires. Este área protegida representa uno de los relictos más australes de la selva en galería, donde el conocimiento de la fauna de insectos es aún limitado. Las capturas se realizaron en ambientes de pastizal y selva en galería, con trampas Malaise y trampas de luz, tipo CDC y embudo. Un total de 4.195 especímenes pertenecientes a nueve géneros y 39 especies de Ceratopogonidae fueron identificados. Se brinda un listado de las especies presentes en el área, y se proveen datos de su distribución geográfica. Veintitrés especies se citan en la Reserva por primera vez, mientras que Dasyhelea mediomunda Minaya, Forcipomyia (Forcipomyia) taragui Marino, Spinelli & Cazorla, Forcipomyia (Microhelea) fuliginosa (Meigen), Stilobezzia (Eukraiohelea) elegantula (Johannsen) y Stilobezzia (Stilobezzia) punctulata Lane se registran por primera vez en la provincia de Buenos Aires. PALABRAS CLAVE. Área protegida. Jejenes. Pastizal. Primer registro. Selva en galería. ABSTRACT. We present the results of the first survey on the Ceratopogonidae from the “Reserva Natural Integral Punta Lara”, in Buenos Aires province. This protected area represents the southernmost relict of gallery forest where the knowledge of the insect fauna is still limited. Catches were conducted in areas of grassland and marginal forest with Malaise traps, CDC light traps and funnel light traps. A total of 4,195 specimens belonging to nine genera and 39 species of Ceratopogonidae were identified. A list of the species present in the protected area is provided, along with their distributional data. Twenty-three species are recorded from the reserve for the first time, while Dasyhelea mediomunda Minaya, Forcipomyia (Forcipomyia) taragui Marino, Spinelli & Cazorla, Forcipomyia (Microhelea) fuliginosa (Meigen), Stilobezzia (Eukraiohelea) elegantula (Johannsen) and Stilobezzia (Stilobezzia) punctulata Lane are recorded for the first time in Buenos Aires province. KEYWORDS. Biting midges. First record. Gallery forest. Grassland. Protected area. INTRODUCTION a large and diverse family of small flies. They are common inhabitants of fresh and saline lentic and lotic The Ceratopogonidae or biting and predaceous ecosystemsonallcontinentsexceptAntarctica(Borkent midges, commonly known in Argentina as "jejenes", are & Wirth, 1997). There are more than 1,100 known Copyright Cazorla, C.G. et al.- This is an open access article distributed under the terms of the Creative Commons Attribution Licence (CC BY 4.0) 1 Revista de la Sociedad Entomológica Argentina 77(3): 1-13, 2018 MATERIAL AND METHODS species in the Neotropical region, while in Argentina at Study site least 255 species belonging to 32 genera have been recorded, 47 of which were found in the province of The Reserva Natural Integral Punta Lara (34º 47’ 18” Buenos Aires (Borkent & Spinelli, 2007; Spinelli et al., S; 58º 00’ 01” W) has an extension of 6,000 ha in 2010; Marino et al., 2011; Ronderos et al., 2011, northeast of Buenos Aires province (Fig. 1), on the coast 2017; Borkent, 2016; Cazorla, 2016). of the estuary of the Río de La Plata within the localities From a biogeographical point of view, Argentina of Ensenada and Berazategui. The plant communities spans over both the Andean and Neotropical regions, that make up the Reserve are forest, wetlands and including part of the South American transition zone grasslands. The forest extends along streams and river (Morrone, 2014). In the Neotropical region, the banks, which earned it the name of marginal forest. The Chacoan sub–region includes among others the wetlands are composed of riverside scrubland, lagoons, Chacoan dominion, which is divided in Argentina into streams, clipper grassland, lilygrass and channels. The two provinces: Chaco and Pampean provinces. The grassland occupies much of the Reserve with a marked Reserva Natural Integral Punta Lara (RNIPL) is located heterogeneity of environments determined by in the Pampean province. Moreover, from an ecological geomorphological and edaphic differences (Herrera & point of view the RNIPL occupies two ecoregions: Delta Torres Robles, 2012). e Islas del Paraná and Pampa (Dinerstein et al., 1995). The RNIPL was declared main area of the Biosphere Data collection Reserve "Pereyra Iraola" (UNESCO, 2017) and it is a Catches of adult Ceratopogonidae were conducted protected natural area within a system of protected between July 2014 and February 2017 with CDC light areas of the province. It was created in 1949 and its traps, funnel light traps, and Malaise traps. Traps were main roles are the conservation of the biodiversity of the installed in different points within the marginal forest and southernmost relict of the gallery forest, the in the neighboring open grassland. CDC light traps were environmental education and interpretation, and the installed in the marginal forest and worked for fifteen scientific research. continuous hours from sunset, once a month during one Despite being the most diverse animal group, year (September 2014 to October 2015); Malaise and arthropods have been and remain minimally funnel traps were installed in the marginal forest as well represented in biodiversity inventories; insects, as the as in the grassland, and worked during two continuous most species–rich and ecologically varied and complex weeks in each point, every month along one year components of animal biodiversity, cannot be (February 2016 to March 2017), although collector cup disregarded in assessing impacts of the environmental removal was carried out every seven days. In the changes (New, 2012). Knowledge about the insect laboratory, specimens were separated by genus with the fauna of the RNIPL is limited. Most systematic and aid of a stereo microscope. To identify them, at least one ecological studies date back to the year 2000, such as male and one female of each species were mounted on the ones concerning the orders Coleoptera (e.g.: slides with Canada balsam according to Borkent & Fernández, 1995; Von Ellenrieder & Fernández, 2000), Spinelli (2007), and identified based on diagnostic keys Odonata (e.g.: Rodrigues Capítulo, 1988, 1996; and original descriptions. The remaining specimens Rodrigues Capítulo & Muzón, 1990; Von Ellenrieder, were preserved in ethyl alcohol 70%. For the recorded 2000), and Heteroptera (e.g.: Schnack et al., 1986, species the citation of the original description is 1989; Von Ellenrieder & Perez Goodwyn, 2000). As presented, followed by the previous records from regards to the Diptera, only three families have been Buenos Aires province. The known distribution of the briefly studied in this area: Ceratopogonidae (Spinelli et species refers to the political division of Argentina into al., 1989), Chironomidae (Donato & Paggi, 2005; Siri et provinces where the species have been recorded in the al., 2008; Mauad et al., 2013) and Culicidae (e.g.: literature (Table I), plus the detailed localities in the case García et al., 1994, 1995; Maciá et al., 1995, 1997; of Buenos Aires province. The order of taxa follows the Campos & Zavortink, 2010). In order to manage and Neotropical catalog of Ceratopogonidae (Borkent & evaluate the effectiveness of protected areas to Spinelli, 2007). Studied specimens are deposited in the preserve biodiversity, it is important to have a Entomological collection of the Museo de La Plata, comprehensive knowledge of the species under Buenos Aires, Argentina (MLP). protection (Vieira et al., 2013). This study presents an updated list of species of Ceratopogonidae from the RESULTS Reserva Natural Integral Punta Lara, in the province of Buenos Aires, Argentina. We list all taxa determined to species level, belonging to subfamilies Forcipomyiinae, Dasyheleinae and Ceratopogoninae. 2 CAZORLA, C.G. et al. Ceratopogonidae from Reserva Natural Integral Punta Lara Family Ceratopogonidae Atrichopogon (Atrichopogon) balseiroi Spinelli, 1982 Subfamily Forcipomyiinae Lenz, 1934 Atrichopogon balseiroi Spinelli, 1982: 206 (male, female; Argentina). Genus Atrichopogon Kieffer Known distribution: Argentina: Buenos Aires: Berisso and RNIPL (new record). Atrichopogon (Atrichopogon) albinensis Ingram & Material examined: 1♂, marginal forest, 3-4/II/2015, Macfie, 1931 CDC light trap; 2♀ 1♂, marginal forest, 15/V-6/VI-2015, Atrichopogon albinensis Ingram & Macfie, 1931: 228 funnel light trap, 11♀ 1♂, same data except 22/II-1/III/ (male, female; Argentina); Ronderos et al., 2011: 1186. 2017. Cazorla, C. G. & Campos, R. E. cols. Known distribution: Argentina: Buenos Aires: Bahía Blanca, Isla Martín García and RNIPL (new record). Atrichopogon (Atrichopogon) comechingon Material examined: 2♀, marginal forest, 10-11/XII/ Spinelli & Marino, 2006 2014, CDC light trap; 1♀, marginal forest, 22/II-1/III/ Atrichopogon (Atrichopogon) comechingon Spinelli & 2017, funnel light trap; 3♀ 1♂, same data except 7-14/ Marino, in Spinelli et al., 2006: 306 (male, female; VIII/2016, Malaise trap; 2♀, grassland, 22-29/VI/2016, Argentina); Marino et al., 2011: 200. Malaise trap; 1♀, same data except 27/VII-3/VIII/2016; Known distribution: Argentina: Buenos Aires: RNIPL 1♂, same data except 17-24/X/2016; 4♀, same data (new record) and Sierra de la Ventana; Córdoba, La Pampa, La Rioja, San Luis and Río Negro. except 24-31/VIII/2016; 1♀, same data except 2-9/III/ Material examined: 2♀, marginal forest, 9-11/IX/2014, 2016, funnel light trap. Cazorla, C. G. & Campos, R. E. CDC light trap; 1♀, same data except 6-7/IX/2014; 1♂, cols. same data except 12-13/III/2015; 1♀, marginal forest, 12-18/II/2016, Malaise trap. Cazorla, C. G. & Campos, R. E.cols. Fi g. 1. Geogra phical l ocation o f Reserva Natura l Integra l Pu nta Lara a nd s ampling site s i n B uenos Aire s province, Argentina. 3 Revista de la Sociedad Entomológica Argentina 77(3): 1-13, 2018 Atrichopogon (Lophomyidium) delpontei Cavalieri & Chiossone, 1972 Atrichopogon (Atrichopogon) homofacies Atrichopogon delpontei Cavalieri & Chiossone, 1972: Spinelli, 1989 121 (male, female; Argentina); Spinelli & Balseiro, 1982: Atrichopogon homofacies Spinelli, in Spinelli et al., 226. 1989: 734 (male, female; Argentina); Ronderos et al., Atrichopogon (Lophomyidium) delpontei: Marino et 2011: 1186. al., 2017: 2082. Known distribution: Argentina: Buenos Aires: Isla Known distribution: Argentina: Buenos Aires: Berisso Martín García and RNIPL. and RNIPL (new record), Formosa, Chaco and Santa Fe. Material examined: 2♀ 2♂, marginal forest, 9-10/IX/ Brazil 2014, CDC light trap; 2♀ 1♂, same data except 3-10/ Material examined: 1♀ 1♂, marginal forest 9-10/IX/ VIII/2016, Malaise trap; 1♀ 2♂, same data except 10-17/ 2014, CDC light trap; 1♀ 1♂, same data except 10-11/ VIII/2016; 10♀ 9♂, grassland, 12-19/X/2016, Malaise XII/2014; 1♀, marginal forest, 18-25/I/2017, funnel light trap; 2♂, same data except 8-15/II/2017. Cazorla, C. G. trap; 1♀, same data except 25/I-1/II/2017; 1♀, & Campos, R. E. cols. grassland, 14-21/XII/2016, funnel light trap; 2♀ 3♂, marginal forest, 30/XI-7/XII/2016, funnel light trap. Atrichopogon (Atrichopogon) seudoobfuscatus Cazorla, C. G. & Campos, R. E. cols. Spinelli, 1982 Atrichopogon seudoobfuscatus Spinelli, 1982: 208 Atrichopogon (Psilokempia) domizii Spinelli, 1982 (male, female; Argentina). Atrichopogon domizii Spinelli, 1982: 206 (male, Known distribution: Argentina: Buenos Aires: Berisso female; Argentina). and RNIPL (new record). Atrichopogon (Psilokempia) domizii: Spinelli et al., Material examined: 2♂, marginal forest, 9-10/X/2014, 2015: 18. CDC light trap; 1♀, same data except 6-7/X/2014; 1♀ Known distribution: Argentina: Buenos Aires: Berisso, 1♂, same data except 10-11/XII/2014; 1♂, grassland, Campana and RNIPL (new record). 7-14 XII/2016, Malaise trap; 1♂, marginal forest, 23-30/ Material examined: 1♂, marginal forest, 10-11/XII/ XI/2016, funnel light trap. Cazorla, C. G. & Campos, R. 2014, CDC light trap. Cazorla, C. G. & Campos, R. E. cols. E.cols. Genus Forcipomyia Meigen Atrichopogon (Atrichopogon) talarum Spinelli, 1982 Forcipomyia (Euprojoannisia) platensis (Brèthes, Atrichopogon talarum Spinelli, 1982: 204 (male, 1914) female; Argentina). Euprojoannisia platensis Brèthes, 1914: 156 (female; Knowndistribution:Argentina:BuenosAires:Berisso Argentina). and RNIPL. Forcipomyia (Euprojoannisia) platensis Brèthes: Wirth Material examined: 3♀ 1♂, marginal forest, 10-11/ & Cavalieri, 1975: 125 (combination); Spinelli, 1983a: XI/2014, CDC light trap; 1♂, same data except 9-10/ 127. XII/2014; 1♂, same data except 12-13/III/2015; 1♀ 1♂, Known distribution: Argentina: Buenos Aires: Berisso, same data except, 23-30/XI/2016, funnel light trap; 2♀ Escobar, General Urquiza, RNIPL (new record) and 3♂, same data except 30/X-7/XI/2016; 8♀ 9♂, same Tigre. dataexcept22/II-1/III/2017,Malaisetrap;1♀,grassland, Material examined: 1♀, marginal forest, 12-13/III/ 7-14/XII/2016, Malaise trap; 1♀ 1♂, same data except 2015, CDC light trap; 1♀, same data except 7-8/V/2015; 11-18/I/2017,funnellighttrap.Cazorla,C.G.&Campos, 4♀, grassland, 4-21/XII/2016, Malaise trap; 2♀, same R. E. cols. data except 16-23/XI/2016. Cazorla, C. G. & Campos, R. E.cols. Atrichopogon(Atrichopogon) sp. nov. A Material examined: 2♀, marginal forest 6-7/X/2014, Forcipomyia (Forcipomyia) pictoni Macfie, 1938 CDC light trap; 38♀ 20♂, same data except 10-11/XI/ Forcipomyia pictoni Macfie, 1938: 161 (male; 2014; 8♀ 2♂, same data except 7-8/I/2015; 3♂, same Trinidad). data except 3-4/2015, 1♀, grassland, 4-12/V/2016, Forcipomyia (Forcipomyia) pictoni: Wirth, 1974: 6; Malaise trap; 2♀, same data except 12-19/X/2016; 1♀ Ronderos et al., 2011: 1186. Known distribution: Argentina: Buenos Aires: Isla 1♂, marginal forest, 23-30/XI/2016, funnel light trap. Martín García and RNIPL (new record), Corrientes, Cazorla, C. G. & Campos, R. E. cols. Formosa, La Rioja and Misiones. Belize, Brazil, Honduras, Costa Rica, Jamaica, Mexico, Panama, Trinidad, United States of America and Venezuela. 4 CAZORLA, C.G. et al. Ceratopogonidae from Reserva Natural Integral Punta Lara Material examined: 1♀, marginal forest, 22/II-1/III/ 1999a: 448 (male, female; Argentina). 2017, funnel light trap. Cazorla, C. G. & Campos, R. E. Known distribution: Argentina: Buenos Aires: RNIPL cols. (new record), Corrientes and Misiones. Paraguay and Uruguay. Forcipomyia(Forcipomyia)pinamarensisSpinelli, First record in Buenos Aires province. 1983 Material examined: 1♀, marginal forest, 10-11/XI/ Forcipomyia (Forcipomyia) pinamarensis Spinelli, 2014, CDC light trap; 1♀, same data except 3-4/II/ 2015; 1983a: 121 (male, female; Argentina). 5♀ 1♂, same data except 12-13/III/ 2015; 1♀, marginal Known distribution: Argentina: Buenos Aires: forest, 13-20/2016, Malaise trap; 1♀ 3♂, marginal forest, Campana, Pinamar and RNIPL (new record). Brazil, 22-II-1/III/2017, funnel light trap. Cazorla, C. G. & Costa Rica, Panama, Uruguay and Venezuela. Campos, R. E. cols. Material examined: 16♀ 4♂, marginal forest 9-10/IX/ 2014,CDClighttrap;2♀,samedataexcept6-7/X/2014; Forcipomyia (Lepidohelea) edmistoni Wirth & 4♂, same data except 10-11/XII/2014; 1♀ 1♂, same Spinelli, 1993 dataexcept13-20/IV/2016,Malaisetrap;7♀,samedata Forcipomyia (Lepidohelea) edmistoni Wirth & Spinelli, except funnel light trap; 33♀ 8♂, grassland, 9-16/2016, 1993: 624 (larva, pupa, male, female; USA); Spinelli & Marino, 1998: 39 (Argentina record). Malaise trap; 10♀ 8♂, same data except 27/IV-4/V/ Known distribution: Argentina: Buenos Aires: RNIPL. 2016; 9♀ 34♂, marginal forest, 22/II-1/III/2017, funnel United States of America. light trap; 1♂, same data except Malaise trap. Cazorla, Material examined: 1♂, marginal forest, 9-1/IX/2014, C.G. & Campos, R. E. cols. CDC light trap; 1♀, same data except 10-11/XI/2014; 1♀, same data except 10-11/XII/2014; 1♀, same data Forcipomyia (Forcipomyia) poulaineae Ingram & except 7-14/2016, Malaise trap; 2♀ 1♂, same data Macfie, 1931 except 30/XI-7/XII/2016. Cazorla, C. G. & Campos, R. E. ForcipomyiapoulaineaeIngram & Macfie, 1931: 227 cols. (male; Argentina). Forcipomyia (Forcipomyia) poulaineae: Spinelli & Forcipomyia (Metaforcipomyia) williamsi Marino & Balseiro,1982:226;Spinelli1983a:124;Ronderosetal., Spinelli, 1999 2011: 1187;Marino et al., 2011: 202. Forcipomyia (Metaforcipomyia) williamsi Marino & Knowndistribution:Argentina:BuenosAires:Buenos Spinelli, 1999b: 5 (female, male; Argentina). Airescity,Berisso,IslaMartínGarcía,RNIPLandSierra Known distribution: Argentina: Buenos Aires: Berisso, de la Ventana; Entre Ríos. Brazil. Isla Martín García and RNIPL. Material examined: 2♀, grassland, 27/III-4/V/2016, Material examined: 5♀, marginal forest, 6-7/X/2014, Malaisetrap;3♀3♂,samedataexcept24-31/VIII/2016; CDC light trap. Cazorla, C. G. & Campos, R. E. cols. 1♂,marginalforest,13-20/VII/2016,funnellighttrap;1♀, samedataexcept3-10/VIII/2016,Malaisetrap.Cazorla, Forcipomyia (Microhelea) fuliginosa (Meigen, C.G. & Campos, R. E. cols. 1818) Ceratopogon fuliginosus Meigen, 1818: 86 (female; Forcipomyia(Forcipomyia) rioplatensisMarino & Germany). Spinelli, 2002 Forcipomyia fuliginosa Goetghebuer, 1933: 130 Forcipomyia squamitibia Lutz: Spinelli, 1983a: 128 (combination). (female, male; Argentina). Forcipomyia (Microhelea) fuliginosa (Meigen): Wirth, Forcipomyia (Forcipomyia) rioplatensisMarino & 1972: 567. Spinelli,2002:314(male,female;Argentina);Spinelliet al.,2005:109;Ronderosetal.,2011:1187;Marinoetal., Forcipomyia crudelisKnab, 1914: 66. 2011: 202. Ceratopogon tropica(Kieffer), 1917: 297. Knowndistribution:Argentina:BuenosAires:Berisso, Known distribution: Argentina: Buenos Aires: RNIPL (new record) and Misiones. Brazil, Colombia, Costa Isla Martín García, RNIPL, San Vicente, Sierra de la Rica, Trinidad, Uruguay and United States of America. Ventana, and Córdoba. Uruguay. Widespread in Palearctic, Oriental and Afrotropical Material examined: 4♀ 1♂, grassland, 12-19/X/2016, regions. Malaise trap; 1♀ 3♂, same data except 1-8/II/2017. First record in Buenos Aires province. Cazorla, C. G. & Campos, R. E. cols. Material examined: 2♀, marginal forest, 10-11/XI/ Forcipomyia(Forcipomyia)taraguiMarino,Spinelli 2014, CDC light trap; 3♀, same data except 10-11/XII/ & Cazorla, 2002 2014; 1♀, same data except 7-8/I/2015; 1♀, same data Forcipomyia(Forcipomyia)taraguiMarinoetal,2002: except 3-4/II/2015; 1♂, same data except 12-13/III/2015. 17 (new name for guarani). Cazorla, C. G. & Campos, R. E. cols. Forcipomyia(Forcipomyia)guaraniMarino&Spinelli, 5 Revista de la Sociedad Entomológica Argentina 77(3): 1-13, 2018 Forcipomyia (Saliohelea) leei Wirth & Dasyhelea suareziSpinelli & Ronderos, 1987 Ratanaworabhan, 1978 Dasyhelea suarezi Spinelli & Ronderos, 1987: 11 Forcipomyia (Saliohelea) leei Wirth & (male, female; Argentina). Ratanaworabhan, 1978: 498 (male, female, pupa; Knowndistribution:Argentina:BuenosAires:Berisso Colombia);Marino et al., 2011: 202. and RNIPL (new record). Known distribution: Argentina: Buenos Aires: RNIPL Material examined: 1♂, marginal forest, 7-8/I/ 2015, (newrecord)andSierradelaVentana.Brazil,Colombia, CDC light trap. Cazorla, C. G. & Campos, R. E. cols. Costa Rica, Puerto Rico and United States of America. Materialexamined:1♀,marginalforest,8-9/VI/2015, Subfamily Ceratopogoninae Newman, 1834 CDC light trap. Cazorla, C.G. & Campos, R.E. cols. Tribe Culicoidini Kieffer, 1911 Forcipomyia(Thyridomyia)nana(Macfie, 1939) Lasiohelea nanaMacfie, 1939: 171 (female; Brazil). Genus Culicoides Latreille Forcipomyia (Thyridomyia) nana (Macfie): Wirth, 1956: 247 (combination);Spinelli et al., 2010: 130. Culicoides (Cotocripus) caridei (Brèthes, 1912) Known distribution: Argentina: Buenos Aires: RNIPL. Cotocripus caridei Brèthes, 1912: 451 (female; Brazil, Colombia, Costa Rica, Dominica, Ecuador, Argentina). Guatemala, Jamaica, Mexico, Panama, Trinidad and Culicoides caridei (Brèthes): Lane, 1945: 366; Spinelli Virgin Islands. et al., 1989: 735; Ronderos & Spinelli, 1990: 83; Material examined: 1♂, marginal forest, 3-4/II/2015, Ronderos et al., 2011: 1187. CDC light trap. Cazorla, C. G. & Campos, R. E. cols. Centrorrhynchus setiferLutz, 1913: 64. Dasyhelea carideiLane & Ortíz, 1950: 460. Subfamily Dasyheleinae Lenz, 1934 Knowndistribution:Argentina:BuenosAires:Berisso, Coronel Pringles, La Plata (Gonnet), Isla Martín García, Genus Dasyhelea Kieffer Magdalena and RNIPL; Jujuy. Uruguay. Materialexamined:60♀72♂,marginalforest,30/XI-7/ Dasyhelea mediomunda Minaya, 1978 XIII/2016,funnellighttrap;67♀,4♂,grassland,7-14/XII/ Dasyhelea mediomunda Minaya, 1978: 79 (male, 2016,funnellighttrap;5♀,samedataexcept14-21/XIII/ female, pupa; Peru). 2016;1♀,samedataexcept26/V-6/VI/2016.Cazorla,C. Known distribution: Argentina: Buenos Aires: RNIPL G.& Campos, R. E. cols. (new record), Chubut, Neuquén and Rio Negro. Chile. First record in Buenos Aires province Culicoides (Haematomydium) debilipalpis Lutz, Material examined: 1♂, grassland, 9-16/III/2016, 1913 Malaise trap. Cazorla, C. G. & Campos, R. E. cols. CulicoidesdebilipalpisLutz1913:60(female,Brazil); Ronderos et al., 2011: 1188. Dasyhelea necrophila Spinelli & Rodríguez, 1999 Culicoides ichesiRonderos & Spinelli, 1995: 77. Dasyhelea necrophila Spinelli & Rodríguez, 1999: 59 CulicoideskhalafiBeck, 1957: 104. (male, female, pupa, larva; Argentina); Ronderos et al., Culicoides lahilleiSpinelli & Wirth, 1986: 62. 2011: 1187; Marino et al., 2011: 203. Knowndistribution:Argentina:BuenosAires:Berisso, Known distribution: Argentina: Buenos Aires: Bahía Isla Martín García and RNIPL; Corrientes, Entre Ríos, Blanca, Berisso, La Plata (Gonnet), Isla Martín García, Jujuy, Misiones, Salta and Tucuman. Belize, Brazil, RNIPL (new record) and Sierra de la Ventana. Colombia, Costa Rica, Honduras, Guatemala, Panama, Material examined: 1♂, grassland, 9-16/III/2016, Peru,Trinidad,Uruguay,VenezuelaandUnitedStatesof Malaise trap. Cazorla, C. G. & Campos, R. E. cols. America. Material examined: 6♀ 1♂, marginal forest, 30/XI-7/ Dasyhelea serrana Díaz & Spinelli, 2014 XII/2016, Malaise trap. Cazorla, C. G. & Campos, R. E. Dasyhelea serrana Díaz & Spinelli, in Díaz et al., cols. 2014: 2153 (male, female, pupa; Argentina). Known distribution: Argentina: Buenos Aires: RNIPL Culicoides(Hoffmania)insignisLutz, 1913 (new record) and Sierra de la Ventana; Río Negro. Culicoides insignis Lutz 1913: 51 (male, female, Material examined: 1♂, marginal forest, 10-11/XIII/ pupa; Brazil); Spinelli & Balseiro, 1982: 226; Spinelli et 2014, CDC light trap. 1♂, grassland, 27/III-4/IV/2016, al., 1989: 735;Ronderos et al., 2011: 1188. Malaise trap; 5♂, same data except 7-14/XIII/2016; Culicoides inamollaeFox & Hoffman, 1944: 110. 1♀1♂, same data except 1-8/III/2017, Malaise trap. Culicoides painteriFox, 1946: 257. Cazorla, C. G. & Campos, R. E. cols. Knowndistribution:Argentina:BuenosAires:Berisso, Isla Martín García and RNIPL; Chaco, Corrientes, Entre Ríos, Formosa, Misiones, Jujuy, Salta and Tucumán. 6 CAZORLA, C.G. et al. Ceratopogonidae from Reserva Natural Integral Punta Lara Brasil, Cayman Islands, Colombia, Costa Rica, Cuba, Material examined: 5♀ 1♂, marginal forest, 10-11/XII/ Dominica, Dominican Republic, Ecuador, French 2014, CDC light trap; 3♀, same data except 22/II-1/III/ Guiana, Guadeloupe, Haiti, Honduras, Jamaica, Mexico, 2017, funnel light trap; 5♀, 3♂, 29/I-5/II/2016, grassland, Panama, Paraguay, Puerto Rico, St. Lucía, Trinidad, Malaise trap; 1♂, same data except 19-26/XII/2016, Venezuela, United States of America and Uruguay. funnel light trap; 1♀, same data except Malaise trap. Material examined: 1♀, marginal forest, 10-11/XI/ Cazorla, C. G. & Campos, R. E. cols. 2014, CDC light trap; 6♀ 1♂, same data except 10-11/ XII/2014; 1♀, same data except 3-4/II/2015; 1♀, same Genus Stilobezzia Kieffer data except 12-13/III/2015; 1♀, same data except 6-7/ V/2015; 1♂, marginal forest, 12-18/II/2016, funnel light Stilobezzia (Eukraiohelea) elegantula (Johannsen, trap; 1♀, grassland, 26/V-6/VI/2016, Malaise trap; 1♂, 1907) same data except, 14- 21/XII/2016, funnel light trap. Bezzia elegantula Johannsen, 1907: 109 (female; Cazorla, C. G. & Campos, R. E. cols. United States of America). Probezzia elegantula: Malloch, 1914a: 137. Culicoides (Psychophaena) venezuelensis Ortíz & Parabezzia elegantula Malloch, 1915: 359. Mirsa, 1950 Parabezzia (Eukraiohelea) elegantula Johannsen, Psychophaena pictipennis (Philippi), 1865: 628 1934: 345. (female; Chile). Eukraiohelea elegantula Johannsen, 1943: 781. Culicoides venezuelensis Ortíz & Mirsa, 1950: 137 Stilobezzia (Eukraiohelea) elegantula Wirth, 1953: 62. (male, female; Venezuela); Wirth, 1955: 234 (= Stilobezzia (Eukraiohelea) maculitibia Lane & Forattini, pictipennis Philippi 1865, non Steager, 1839; = ortizi 1956: 207. Fox); Spinelli & Balseiro 1982: 226; Spinelli & Wirth, Stilobezzia subsessilisKieffer, 1917: 311. 1986: 64; Spinelli et al., 1989: 735; Ronderos et al., 2011: Known distribution: Argentina: Buenos Aires: RNIPL 1189; Marino et al., 2011: 203. (new record), Chaco and Misiones. Brazil, Culicoides ortiziFox, 1952: 366. Mexico, Panama, Paraguay, (?) Puerto Rico and United Knowndistribution:Argentina:BuenosAires:Berisso, States of America. Isla Martín García, RNIPL and Sierra de la Ventana; First record in Buenos Aires province. Catamarca, Chaco, Córdoba, Corrientes, Entre Ríos, Material examined: 1♀, marginal forest, 10-11/XII/ Formosa, Jujuy, Mendoza, Misiones, Río Negro, Salta, 2014, CDC light trap. Cazorla, C. G. & Campos, R. E. SantiagodelEsteroandTucumán.Brazil,Bolivia,Chile, cols. Colombia, Costa Rica, Ecuador, Panamá, Paraguay, Uruguay and Venezuela. Stilobezzia (Stilobezzia) bicolor Lane, 1947 Material examined: 1♀, marginal forest, 10-11/XI/ Stilobezzia (Stilobezzia) bicolor Lane, 1947: 208 2014,CDClighttrap;15♀13♂,samedataexcept10-11/ (male, Brazil); Spinelli, 1983b: 410. XII/2014; 2♂, same data except 7-8/I/ 2015; 23♀ 2♂, Known distribution: Argentina: Buenos Aires: Berisso and RNIPL (new record). Brazil and Panama. samedataexcept3-4/II/2015;1♀2♂,samedataexcept Material examined: 1♀, marginal forest, 12-13/III/ 12-13/III/2015; 2♀ 1♂, same data except 6-7/V/2015; 2015, CDC light trap. Cazorla, C. G. & Campos, R. E 5♀ 7♂, same data except, 22/II-1/III/2017, funnel light cols. trap; 27 ♀ 1♂, same data except 23-30/XI/2016; 18♀ 1♂, same data except 30/XI-7/XII/2016; 2♀; same data Stilobezzia (Stilobezzia) fiebrigi Kieffer, 1917 except 28/XII- 4/VI/2017; 5♀ 7♂, same data except 22/ Stilobezzia fiebrigi Kieffer, 1917: 309 (female, II-1/III/2017; 1♀, grassland, 9-16/III/2016, Malaise trap; Paraguay). 1♀, same data except 14-21/XII/2016, funnel light trap; Stilobezzia (Stilobezzia) fiebrigi Forattini & Rabello, 1♂, same data except 8-15/II/2016, Malaise trap. 1956: 45; Spinelli & Balseiro, 1982: 226; Spinelli, 1983b: Cazorla, C. G. & Campos, R. E. cols. 410. Known distribution: Argentina: Buenos Aires: Berisso, Tribe Ceratopogonini RNIPL and Tigre, Corrientes, Entre Ríos and Santiago del Estero. Brazil and Paraguay. Genus Alluaudomyia Kieffer Material examined: 1♀ 1♂, marginal forest, 10-11/XI/ 2014, CDC light trap; 1♀ 6♂, same data except 10-11/ Alluaudomyia schnacki Spinelli, 1983 XII/2014; 1♀ 2♂, same data except 7-8/I/2015; 1♂, same Alluaudomyia schnacki Spinelli, 1983b: 403 (larva, data except 12-13/III/2015; 1♀, same data except 6-7/ pupa, male, female; Argentina); Spinelli et al., 1989; IV/2015; 1♀, same data except 30/XI-7/XII/2016, funnel Marino et al., 2011: 203; Díaz et al., 2015: 89. light trap; 1♀, same data except 12-18/II/2016; 1♀ 1♂, Known distribution: Argentina: Buenos Aires: Sierra same data except 22/II-1/III/2017; 1♀, same data except de la Ventana, Berisso, Escobar and RNIPL; Chubut, 30/XI-7/XII/2016. Cazorla, C. G. & Campos, R. E. cols. Corrientes, Entre Ríos, Formosa. Chile and Uruguay. 7 Revista de la Sociedad Entomológica Argentina 77(3): 1-13, 2018 Stilobezzia (Stilobezzia) glauca Macfie, 1939 Germany). Stilobezzia glauca Macfie, 1939: 204 (male; Brazil). Bezzia nobilis (Winnertz): Kieffer, 1901: 153 Stilobezzia fluminensis Lane, 1947: 210. (combination; in key). Stilobezzia (Stilobezzia) glauca Wirth & Grogan, 1981: Bezzia(Bezzia) nobilis: Wirth, 1983: 673 (review of 81; Cazorla, 2016: 188. New Worldnobilisgroup);Spinelli & Wirth, 1989: 112. Known distribution: Argentina: Buenos Aires: RNIPL, Bezzia acanthodesMacfie, 1940: 192. and Misiones. Brazil, Colombia, Mexico, Peru and Known distribution: Argentina: Buenos Aires: Berisso, United States of America. Magdalena and RNIPL (new record); Río Negro. Material examined: 1♀, marginal forest, 7-8/I/2015, Bahamas, Bermuda, Brazil, British Guiana, Canada, CDC light trap; 1♀ 1♂, same data except 3-4/II/2015. Chile, Cuba, Haiti, El Salvador, Honduras, Jamaica, Mexico, United States of America, Uruguay, Virgin Cazorla, C. G. & Campos, R. E. cols. Islands and Paleartic Region. Material examined: 1♂, marginal forest, 10-11/XII/ Stilobezzia (Stilobezzia) kiefferi Lane 1947 2014, CDC light trap; 1♀, same data except, 25/I-1/II/ Stilobezzia kiefferi Lane, 1947: 205 (female; Brazil). 2017, funnel light trap; 2♂, same data except, 30/I-7/II/ Stilobezzia (Stilobezzia) punctipes Wirth, 1953: 79. Stilobezzia (Stilobezzia) kiefferi Lane & Forattini, 1961: 2016; 4♀ 1♂, 28/XII- 4/I/2017; 11♂, same data except 85; Cazorla, 2016: 188. 22/II-1/III/2017. Cazorla, C. G. & Campos, R. E. cols. Known distribution: Argentina: Buenos Aires: RNIPL. Brazil, Jamaica, Mexico, Panama and United States of Bezzia punctipennis (Williston, 1896) America. Ceratopogon punctipennis Williston, 1896: 278 Material examined: 1♂, 10/XI/2014, CDC light trap; (female; St. Vicent). 2♀, same data except 10/XII/2014. Cazorla, C. G. & Bezzia punctipennis (Williston): Kieffer, 1906: 58 (combination); Spinelli & Wirth, 1989: 119. Campos, R. E. cols. Known distribution: Argentina: Buenos Aires: Berisso, Stilobezzia (Stilobezzia) punctulata Lane, 1947 Magdalena and RNIPL (new record). Brazil, Dominica, El Stilobezzia punctulata Lane, 1947: 204 (female; Salvador, Haiti, Honduras, Jamaica, Mexico, St. Vincent and United States of America. Brazil). Stilobezzia (Stilobezzia) punctulata Lane & Forattini, Material examined: 1♀, marginal forest, 10-11/XII/ 1958: 220. 2014, CDC light trap; 1♀, same data except 12-13/III/ Known distribution: Argentina: Buenos Aires: RNIPL 2015; 1♂, same data except 28/XII-4/I/2017, funnel light (new record), Chaco and Corrientes. Brazil, Mexico, trap; 1♀, same data except 30/XI-7/XII/2016, Malaise Panama and Peru. trap; 1♀, same data except 25/I- 1/II/2017, funnel light First record in Buenos Aires province. trap; 1♀, same data except 22/II-1/III/2017, funnel light Material examined: 1♂ 1♀, marginal forest, 10-11/XII/ trap; 1♀, grassland, Malaise trap. Cazorla, C. G. & 2014, CDC light trap. Cazorla, C. G. & Campos, R. E. Campos, R. E. cols. cols. GenusPalpomyiaMeigen Tribu Heteromyiini Wirth, 1962 Palpomyia subaspera(Coquillett, 1901) GenusClinoheleaKieffer CeratopogonsubasperCoquillett,1901:606(female; USA). Clinohelea townsendiLane, 1944 Palpomyia subasper Malloch, 1914b: 22 Clinohelea townsendi Lane, 1944: 256 (female; (combination). Brazil);Spinelli & Duret, 1993: 48. Palpomyiasubaspera (Coquillett): Grogan & Wirth, Clinohelea townesiLane, 1944: 254. 1975: 10 (lectotype designation; redescr.). Known distribution: Argentina: Buenos Aires: RNIPL Palpomyia essigi Wirth, 1952: 225 (male, female; (new record) and Tigre; Corrientes. Brazil, Colombia, USA). Ecuador, French Guiana, Panama, Paraguay and Peru. Palpomyiamaculicrus Ingram & Macfie, 1931: 230 Materialexamined:3♀,marginalforest,12-18/II/2016, (female; Argentina). Malaise trap. Cazorla, C. G. & Campos, R. E. cols. Known distribution: Argentina: Buenos Aires: RNIPL (new record) and San Isidro; Chubut and Río Negro. Tribu Palpomyiini Enderlein, 1936 Canada, Chile, Cuba, Haiti, Mexico, Paraguay and United States of America. GenusBezziaKieffer Materialexamined:2♀,marginalforest,12-18/II/2016, Malaise trap. Cazorla, C. G. & Campos, R. E. cols. Bezzia(Bezzia)nobilis(Winnertz, 1852) Ceratopogon nobilis Winnertz, 1852: 79 (female; 8 CAZORLA, C.G. et al. Ceratopogonidae from Reserva Natural Integral Punta Lara DISCUSSION theprovinceofBuenosAiresisincreasedto39.Twenty- threeofthesespeciesarerecordedintheRNIPLforthe ThelaststudyofthefamilyCeratopogonidaecarried first time, while five species are recorded for the first out in the Reserva Natural Integral Punta Lara dates timeinBuenosAiresprovince:Dasyheleamediomunda, back almost thirty years and listed ten known species Forcipomyia (F.) taragui, Forcipomyia (M.) fuliginosa, and seven undetermined species (Spinelli et al., 1989). Stilobezzia (E.) elegantula and Stilobezzia (S.) In the present study, we present an updated list where punctulata. Most of these species have a Neotropical thenumberofnamedspeciesforthisprotectedareaof distribution; therefore, their presence in this area Table I. Distribution of Ceratopogonidae species collected in the Reserva Natural Integral Punta Lara, by province. ①①: First record in Reserva Natural Integral Punta Lara, ②②: First record in Buenos Aires province. 9 Revista de la Sociedad Entomológica Argentina 77(3): 1-13, 2018 represents the southernmost limit of their distribution, Cavalieri, F., & Chiossone, I.F. (1972) Notas sobre el género while Dasyhelea mediomunda presents Andean Atrichopogon Kieffer, 1906, de la Argentina (Diptera, Ceratopogonidae). I. Consideraciones generales y distribution, so this new record represents the descripcióndeAtrichopogondelponteisp.nov.Revistade easternmost limit of its distribution. Besides, it is noted la Sociedad Entomológica Argentina,3344, 119-129. that the previously published first records of Stilobezzia Cazorla,C.G.(2016)Newrecordsof"jejenes"onthesubgenus (S. ) glauca and Stilobezzia (S .) kiefferi from Buenos Stilobezzia of Stilobezzia from the Neotropical Region Aires province (Cazorla, 2016) were made based on (Diptera: Ceratopogonidae). Revista de la Sociedad material from the same series of samples. Entomológica Argentina,7755((33--44)), 186-190. Forcipomyia and Atrichopogon were the genera with Coquillett, D.W. (1901) New Diptera in the U.S. National higher number of species, 11 and 9 respectively. Museum. Proceedings of the United States National Regarding the genera Culicoides, Monohelea Museum,2233, 593-618. Kieffer, Forcipomyia (subgenera Caloforcipomyia, Díaz, F., Spinelli, G.R., Donato, M., & Ronderos, M.M. (2014) A taxonomic revision of the Patagonian species of the Forcipomyia, Synthyridomyia and Trichohelea) and Dasyhelea mutabilis species group with a phylogenetic Palpomyia, some material remains undetermined and is analysisofNewWorldspecies(Diptera:Ceratopogonidae). being studied, as they could be new taxa. In addition, Journal of Natural History,4488, 2117-2175. an undetermined species of Atrichopogon has resulted Díaz, F., Marino, P.I., Ronderos, M.M., & Spinelli, G.R. (2015) to be a new one, which was previously found by DetaileddescriptionoflarvaeandpupaeoftwoNeotropical Cazorla, Marino and Díaz in Sierra de la Ventana, species of Alluaudomyia Kieffer (Culicomorpha: province of Buenos Aires, and it is currently under Ceratopogonidae).Zoologischer Anzeiger,225577, 87-95. description. Dinerstein,E.,Olson,D.M.,Graham,D.J.,Webster,A.L.,Primm, S.A.,Bookbinder,M.P.,&Ledec,G.(1995)Unaevaluación delestadodeconservacióndelasecoregionesterrestres ACKNOWLEDGEMENTS de América Latina y el Caribe. WWF and World Bank, Washington D.C. We want to express our gratitude to Juan Pablo Carricart, park ranger of the RNIPL and its staff for their Donato, M., & Paggi, A. (2005) A new Neotropical species of the genus Metriocnemusvan der Wulp (Chironomidae: kind help and assistance during the collecting. We also Orthocladiinae)fromEryngiumL.(Apiaceae)phytotelmata. thank Dr. Gustavo Spinelli for the loan of the Malaise Zootaxa,11005500, 1-14. trap and his willingness to our consults, as well as Lic. Fernández,L.A.(1995)PresenciadelsubgéneroHydatotrephis Josefina Otero for her assistance with the figure. This MacLeay en la región Neotropical y descripción de study was supported by grants PIP 0305 and PIP 0399 Enochrus(Hydatotrephis)bachmannisp.nov.(Coleoptera: from Consejo Nacional de Investigaciones Científicas y Hydropilidae).Neotropica,4411((110055––110066)), 51-54. Técnicas. Forattini, O.P., & Rabello E.X. (1956) As formas imaturas de Culicoides guyanensis Floch e Abonnenc, 1942 e de algumas espécies de Stilobezzia (Diptera, Ceratopogonidae). Revista Brasileira de Entomologia, 66, LITERATURE CITED 43-50. Fox, I. (1946) A review of the species of biting midges Beck, E.C. (1957) Two new species of Culicoides from Florida of Culicoides from the Caribbean region (Diptera: (Diptera: Heleidae). Florida Entomologist , 4400, 103-105. Ceratopogonidae). AnnalsoftheEntomologicalSocietyof Borkent, A. (2016) World Species of Biting Midges (Diptera: America,3399, 248-258. Ceratopogonidae). Available in: http://www.inhs.uiuc.edu/ Fox, I. (1952) Six new Neotropical species of Culicoides research/FLYTREE/CeratopogonidaeCatalog.pdf (Diptera: Ceratopogonidae). AnnalsoftheEntomological Borkent, A., & Spinelli, G.R. (2007) Neotropical Society of America,4455, 364-368. Ceratopogonidae(Diptera:Insecta).AquaticBiodiversityin Fox,I.,&HoffmanW.A.(1944)NewNeotropicalbitingsandflies Latin America (ABLA) (ed. Adis, J., Arias, J.R., ofthegenusCulicoides(Diptera:Ceratopogonidae).Puerto Rueda–Delgado,G.,&Wantzen,K.M.),pp1–198.Pensoft, RicoJournalofPublicHealthandTropicalMedicine, 2200, Sofia–Moscow. 108-111 Borkent, A., & Wirth, W.W. (1997) World species of biting García, J.J., Campos, R.E., & Maciá, A. (1994) Prospección midges (Diptera: Ceratopogonidae). Bulletin of the de enemigos naturales de Culicidae (Diptera) de la selva American Museum of Natural History,223333, 1-257. marginaldePuntaLara(Prov.deBuenosAires,República Brèthes,J.(1912)Descripcióndeunnuevogéneroyespecie Argentina).RevistadeLaAcademiadeCienciasExactasy nuevadeChironomidae(Dipt.).AnalesMuseoNacionalde Naturales de Colombia,1199, 209-215. Historia Natural de Buenos Aires,1155, 451-453. García,J.J.,Campos,R.E.,&Maciá,A.(1995)Observaciones Brèthes,J.(1914)DescriptiondesixCecidomyidae(Dipt.)de ecológicas sobre Mansonia indubitans y Ma. titillans BuenosAires.AnalesMuseoNacionaldeHistoriaNaturalde (Diptera: Culicidae) y sus enemigos naturales en Punta Buenos Aires,2266, 151-156. Lara, Argentina. Revista de la Sociedad Entomológica Argentina, 5544((11--44)), 43-50. Campos,R.E.,&Zavortink,T.J.(2010)Descriptionofthelarva and pupa and redescription of the adults of Isostomyia Goetghebuer,M.(1933)CeratopogonidaeetChironomidaedu paranensis (Brèthes) (Diptera: Culicidae). Zootaxa, 22668899, Congo Belge. Revue de Zoologie et de Botanique 27-36. Africaines,2244, 129-151. 10