Journal of the Royal Society of Western Australia, 95: 77-81, Distribution of Westralunio carteri Iredale 1934 (Bivalvia: Unionoida: Hyriidae) on the south coast of southwestern Australia, iricludingnew. records of the species M W KLUNZINGER1*, S J BEATTY1, D L MORGAN1, A J LYMBERY1, A M PINDER2 & D J CALE2 1 Freshwater Fish Group & Fish Health Unit, Murdoch University, Murdoch, WA 6150, Australia. 2 Science Division, Department of Environment and Conservation, Woodvale, WA 6026, Australia. * Corresponding author [email protected] Westralunio carteri Iredale 1934 is the only hyriid in southwestern Australia. The species was listed as 'Vulnerable' by the IUCN, due to population decline from dryland salinity, although the listing was recently changed to 'Least Concern'. The Department of Environment and Conservation lists the species as 'Priority 4', yet it lacks special protection under federal or state legislation. Accuracy in species accounts is an important driver in determining conservation status of threatened species. In this regard, discrepancies in locality names and vagary in museum records necessitated the eastern distributional bounds of W. carteri to be clarified. Here we present an updated account of the species' distribution and describe two previously unknown populations of W. carteri in the Moates Lake catchment and Waychinicup River, resulting in an eastern range extension of 96-118 km from the Kent River, formerly the easternmost river where W. carteri was known. For mussel identification, samples (n = 31) were collected and transported live to the laboratory for examination and internal shell morphology confirmed that the species was W. carteri. Due to an acute salinity tolerance of 3.06 g/L (LD^), the species is unlikely to exist in catchments east of the Waychinicup River where salinities are known to be as high as 45 g/L. The current study clarifies the eastern distribution of W. carteri and will allow monitoring of its range decline to be made in the future. KEYWORDS: freshwater mussel, geographic distribution, Moates Lake, species conservation, Waychinicup River, Westralunio carteri INTRODUCTION a highly seasonal rainfall and climate pattern similar to that of the Mediterranean, with hot dry summers and Freshwater mussels (Bivalvia: Unioniformes) are found cool wet winters (Pen 1999). Many of the formerly in freshwater environments on every continent except freshwater streams, rivers, lakes and wetlands have Antarctica (Bogan & Roe 2008). The life history of these become affected by dryland salinity through a legacy of bivalves is generally characterised by their parasitic land clearing dating back to the late 1800s (Wood 1924; larvae that usually require fish as hosts (Bauer & Beresford el al. 2001). Wachtler 2001; Strayer 2008). Parasitic larvae of the Unionoidea (Hyriidae, Margaritiferidae and Unionidae) Westralunio carteri Iredale, 1934 is the only unioniform are known as 'glochidia', which are morphologically to inhabit the South West Coast Drainage Division of distinct from the 'lasidia' or 'haustoria' of Etherioidea Australia (McMichael & Hiscock 1958; Walker 2004). (Etheriidae, Iridinidae and Mycetopodidae) (Bauer & Kendrick (1976) first noted the disappearance of W. Wachtler 2001), although Graf & Cummings (2006) carteri from the Avon River, and proposed that this placed the glochidia of the Hyriidae in the Etherioidea resulted from secondary salinisation of its formerly based on phylogenetic evidence. freshwater habitat. Later, results of a biodiversity study (Pinder et al. 2004) suggested that W. carteri had The Southern Hemisphere is dominated by the disappeared from parts of its range within the Hyriidae with 76-83 species occurring in Australasia and agricultural zone, strengthening Kendrick's (1976) South America (Walker et al. 2001; Graf & Cummings assessment which resulted in the species being listed as 2006; Bogan & Roe 2008). Australia currently has 18 'Vulnerable' on the International Union for the nominal species (McMichael & Hiscock 1958; Ponder & Conservation of Nature's Red List of Threatened Species Bayer 2004; Walker 2004), although recent genetic (IUCN 1996 in Kohler 2011), but was not listed under investigations have confirmed the existence of additional state or federal legislation. However, the species has species, which have not yet been described (Baker et al. recently been assessed as 'Least Concern' '...as it is 2003, 2004; Hughes et al. 2004). widespread in Western Australia, is a habitat generalist, The high concentration of endemic flora and fauna and is resistant to organic pollution. Although previously unique to southwestern Australia contributes to its listing assessed as Vulnerable (under version 2.3 of the Red List) as one of the world's biodiversity hotspots (Myers et al. under the revised Categories and Criteria this species no 2000). The region is comprised of 19 drainage basins with longer qualifies for a threatened listing.' (Kohler 2011). Westralunio carteri has recently been shown to have an acute salinity LD5(| of 3.06 g/L (Klunzinger et al. 2010). © Royal Society of Western Australia 2012 77 Journal of the Royal Society of Western Australia, 95(2), July 2012 Stewart (2009) proposed two aquatic bioregions for the MATERIALS AND METHODS South Coast Region of Western Australia based largely on salinity and differences in macroinvertebrate The south coast of Western Australia has been heavily compositions, presumably with different salinity cleared since the 1950s and the land is used primarily for tolerance thresholds. In the list of individual species used dryland cropping with some plantation forestry and for the assessment, 'Westralunio spp.' was reportedly some national parks and nature reserves (Mayer et al. found in greater abundance in the Eastern South Coast 2005). We surveyed regional water bodies for W. carteri where salinities are much higher than in the Western by tactile searches in wadeable areas of river and lake South Coast. Inland freshwater lakes of the south coast habitats from 76 sites on the south coast from the Kent were also surveyed for macroinvertebrates by Edward et River to the Pallinup River during March 2011 and al. (1994), but no W. carteri was reported in the study area January 2012 (Figure 1). Where mussels were found, from Kent River to Esperance. patch densities were determined by counting the number of mussels in localised quadrats (1 m2). Records from the Western Australian Museum, Perth (WAM) and the Australian Museum, Sydney (AMS) for Benthic habitat characteristics, including the dominant W. carteri on the south coast east of the Kent River are substrate type and presence/absence of plant debris were sparse and locality descriptions are vague, but include also noted. Specimens were randomly collected from the following: AMS_126151, King George Sound (ca three sites (n = 32) and transported live to the laboratory 1877); WAM_34290, South Stirlings, Calvin River (ca in a 15 L plastic bucket of river water. Shells were 1963); WAM_34289, Esperance Town Beach (ca 1978), but measured as per McMichael & Hiscock (1958) and no coordinates for these localities are available. In the Walker (2004), subsequently euthanased in 0.01% context of accurate assessment of species conservation, benzocaine solution, and dissected to determine sex and here we present updated and new records for W. carteri reproductive status. Gills were examined for the presence on the south coast of Western Australia. (females) or absence (males) of specialised brooding Figure 1 Distribution of Westralunio carteri Iredale 1934 in the south coast region of southwestern Australia. Thick black lines represent river basin boundaries and the dashed line represents the boundary between the eastern and western south coast bioregions as proposed by Stewart (2009). 78 Klunzinger et al.: Westralunio carteri in southwestern Australia chambers within the demibranchs of the gills with patch density rapidly decreasing beyond the mouth ('marsupia') and whether they contained glochidia by the of the Goodga River (Figure 2). The greatest densities of presence or absence of thickened, orange to red coloured mussels were found in slower flowing areas around river marsupia as described for other hyriids (Jones et al. 1986; bends near the banks, nestled amongst submerged tree Byrne 1998). Internal shell morphology was examined roots. Where mussels were found, the substrate was using McMichael & Hiscock (1958) as a guide. Shells and dominated by coarse sand with some woody debris and soft tissues were then preserved in 100% ethanol for leaf litter. Above the Goodga River gauging station, future study. benthic habitats consisted primarily of soft sludge and detritus. Species distributions from Edward et al. (1994), Pinder et al. (2004) and this study were mapped using ArcGIS(cid:8482) Very few mussels were found in the small area of Desktop 10. Linear hydrography and river basin Waychinicup River, but four individuals were located boundary data were obtained under license from the near a submerged log in fine sand and silt. All of the Western Australian Department of Water. Coastal and sites that contained W. carteri had characteristic tannin- administrative boundaries as well as population centres stained water and were fringed by well-vegetated river were mapped from the 'Global Map Australia 1M 2001' banks lined with thick shrubs, and shading trees. dataset (Geoscience Australia 2004). DISCUSSION RESULTS To our knowledge, this study represents the first account Freshwater mussels were found in the lower Kent River of W. carteri from the Moates Lake catchment and and new populations were discovered in the Moates Waychinicup River of the south coast, which together are Lake catchment in Two Peoples Bay Nature Reserve, probably the only remaining populations within the located -30 km east of Albany and in the Waychinicup Albany Coast Basin, although neighbouring streams such River, -22 km east of Moates Lake on the south coast of as the Angove River may also support the species. We Western Australia, but no mussels were found in any of suspect that the current existence of W. carteri on the other waterways sampled (Figure 1). All freshwater Esperance Town Beach (WAM_34289, ca 1978) is unlikely mussels were identified as W. carteri. Marsupia were given that mean salinities of rivers near Esperance are present in the inner demibranchs of females, a greater than 5 g/L, and have been since the late 1970s characteristic feature of the Hyriidae. None of the females (Mayer et al. 2005). Westralunio carteri was recorded from examined contained glochidia. the King George Sound locality in the late 1800s, but the Westralunio carteri was found to occupy a 2.4 km exact location is not available from Australian Museum stretch of Goodga River, but was not recorded above the records. Although a record for the species exists in the fishway at gauging station AWRC 602199 (Figure 2). In 'Calvin River', there is no river by that name in Western Moates Lake, the species was only found within a 300 m2 Australia and was probably made in error. The record area extending beyond the mouth of the Goodga River, probably refers to the Kalgan River, but this has not been but no live mussels were found beyond the river mouth confirmed. If indeed the species did exist in this locality, (Figure 2). Densities of W. carteri ranged from 1 to 15/m2, the new information from this study possibly represents Figure 2 Distribution of Westralunio carteri Iredale 1934 in the Moates Lake catchment of southwestern Australia. Mussel density symbols represent the number of mussels per lm2 quadrat. 79 Journal of the Royal Society of Western Australia, 95(2), July 2012 a range extension of ~8-30km east from Kalgan River. 2003; Morgan & Beatty 2006). If host fishes could not However, our recent surveys failed to record the species deliver attached glochidia upstream of the gauging in the Kalgan River, thereby placing the nearest station and if aging mussels above the weir were unable population 96-118 km to the west in the Kent River. to expose their glochidia to potential hosts, they could have died out from recruitment failure. If we knew the Waychinicup River represents the easternmost limit of longevity of adult W. carteri in this system, had the distribution of endemic freshwater fishes (Morgan et information on suitable host fishes, knew tolerance al. 1998, 2011), which is probably true for W. carteri as thresholds for NH3 and whether we could find juvenile well. Very few, if any perennial rivers east of W. carteri, we would be able to test these hypotheses. Waychinicup are fresh (Mayer el al. 2005; Stewart 2009), Although host fishes in these systems are unknown, with a majority of the rivers in the region having Nannoperca vittata (Castelnau 1873) and Pseudogobius salinities above the known acute tolerance of 8.2-14.6 g/L olprum (Sauvage 1880) which are known to inhabit both for several endemic freshwater fishes (Beatty et al. 2011), Goodga and Waychinicup Rivers (Morgan & Beatty 2006; and 3.06 g/L for W. carteri (Klunzinger et al. 2010), so the Morgan el al. 1998, 2011) are likely candidates given that likelihood of finding freshwater mussels in salinised they are known to support glochidia of W. carteri rivers in the Eastern South Coast is probably low. The elsewhere in its range (Klunzinger et al. in press). The species data from Binder el al. (2004), presented in Figure Goodga River contains Australia's only Critically 1, lends support to this presumption. Although Stewart Endangered fish, Galaxias truttaceus (Cuvier & (2009) reported ‘Westmlunio spp/ occurring in many of Valenciennes 1846) as well as Galaxias maculatus (Jenyns the salinised rivers of the south coast, the species 1842) (Morgan 2003; Stewart 2011; Morgan & Beatty identification was probably erroneous and was most 2006; Morgan et al. 1998, 2006, 2011), both of which may likely Fluviolanatus subtorta (Dunker 1857), based on be hosts for glochidia. photos provided by the former author (B Cook pers. comm. 2010). This trapezoid has migrated inland from The accuracy of species distributions is important for estuaries to salinised rivers of the region such as Avon conservation assessments, particularly at the broad River (Kendrick 1976) and Blackwood River (Pen 1999). regional scale (Anderson & Martinez-Meyer 2004). This We have also observed this species and Mytilus (Mytilus) study adds to the current state of knowledge on the planatus Lamarck 1819, a marine/estuarine mytilid, biogeography of W. carteri, namely extent of occurrence, inhabiting the salinised Kalgan River nearly 40 km which is a key criteria used for conservation assessment inland from the estuary. under the Western Australian Wildlife Conservation Act 1950, the federal Environment Protection and Biodiversity Besides salinity, sedimentation may be causing Conservation Act 1999 and the IUCN Red List of localised declines based on our observations of the Threatened Species. Results from this study will species elsewhere, which is supported by Jones & Byrne undoubtedly be useful in future conservation assessments (2010) who found that sedimentation is a limiting factor of the status of the species. Factors influencing the for eastern Australian hyriids. Reservoir dewatering and occurrence of this species in relation to conservation rainfall reductions appear to be having a negative effect criteria should be the focus of ongoing investigations. on W. carteri populations (M W Klunzinger et al. unpubl. data). The region's climate is changing, most notably since the 1970s with rainfall, surface flow and groundwater reductions, amplifying the effect of dryland ACKNOWLEDGEMENTS salinity and increasing the vulnerability of freshwater This work was funded by a grant from the Holsworth fishes to population loss with the trend expected to Wildlife Research Endowment and an International PhD continue into the future (Morrongiello et al. 2011). Scholarship from Murdoch University. A License to Take Due to limited time and resources during the survey, Fauna for Scientific Purposes (Regulation 17 of the we did not collect an adequate number of mussels to Wildlife Conservation Act 1950) was obtained from the draw any conclusions from shell measurements about Department of Environment and Conservation and a population structure. Because growth rates, host fishes Research Permit (under exemption from the Fish and reproductive biology of W. carteri in these systems Resources Management Act 1994) from the Department have not been quantified, we are reluctant to infer age, of Fisheries. We especially thank James Keleher and Iain reproductive status or recent recruitment patterns. Future Foster for their help during field sampling. We thank assessments of the population structure in these systems two anonymous reviewers for constructive criticism should utilise a more rigorous sampling design to during review of earlier versions of this publication and maximise the probability of collecting the full range of Pierre Horwitz for helpful comments and suggestions size classes and in greater quantity for statistical power. during revision of the manuscript. The absence of W. carteri above the gauging station weir in Goodga River could be due to the build-up of detritus and fine sediments which have been known to REFERENCES cause hypoxia/anoxia and a build-up of un-ionised ammonia (NH3) during summer months and non-flow or Anderson R P & Martinez-Meyer E 2004. 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