wnnmnflininSnlnifiWtimwnnnnTnT Arquivos do Museu Nacional, Rio de Janeiro, v.64, n.4, p.321-324, out./dez.2006 ISSN 0365-4508 DESCRIPTION OF A NEW SPECIES OF PHYLLODYTES WAGLER, 1830 (ANURA, HYLIDAE) FROM THE ATLANTIC RAIN FOREST OF THE STATES OF MINAS GERAIS AND BAHIA, BRAZIL 1 (With 2 figures) CARLOS ALBERTO GONÇALVES CRUZ 2’3 RENATO NEVES FEIO 4 MONICA CARO LI NA DA SILVA CARDOSO 2’5 ABSTRACT: A new species of the genus Phyllodytes is described from the Atlantic Rain Forest of northeastern Minas Gerais and Southern Bahia, Brazil. The new species is distinguished from all other species of the genus by the color pattern of dorsum (body, arms, and legs pale brown with irregular distinctive cream stains) and larger size (SVL 39.7-4-3.5mm in males, 4-8.5mm in female). Key words: Anura. Hylidae. Phyllodytes maculosus sp.nov. Atlantic Rain Forest. Taxonomy. RESUMO: Descrição de uma nova espécie de Phyllodytes Wagler, 1830 (Anura, Hylidae) da Floresta Atlântica dos Estados de Minas Gerais e Bahia, Brasil. Uma nova espécie do gênero Phyllodytes é descrita da Floresta Atlântica no nordeste de Minas Gerais e sul da Bahia. A nova espécie distingue-se das demais espécies do gênero pelo padrão de colorido dorsal (corpo e membros de cor marrom pálido com manchas irregulares de cor creme) e por seu tamanho maior (CRA 39.7-4-3.5mm nos machos, 4-8.5mm na fêmea). Palavras-chave: Anura. Hylidae. Phyllodytes maculosus sp.nov. Floresta Atlântica. Taxonomia. INTRODUCTION MATERIAL AND METHODS Treefrogs of the genus Phyllodytes are distributed Specimens examined are deposited in the in Trinidad and eastern Brazil (Peixoto etal, 2003). collections: EI (Eugênio Izecksohn Collection, Currently, 11 species are recognized: P. auratus Universidade Federal Rural do Rio de Janeiro, (Boulenger, 1917) occurs in Trinidad and P. Brazil), MNRJ (Museu Nacional, Rio de Janeiro, acuminatus Bokermann, 1966, P. brevirostris Brazil), MZUSP (Museu de Zoologia, Universidade Peixoto & Cruz, 1988, P. edelmoi Peixoto, de São Paulo, Brazil), USNM (United States National Caramaschi & Freire, 2003, P. gyrinaethes Peixoto, Museum, Smithsonian Institution, Washington, Caramaschi & Freire, 2003, P. kautskyi Peixoto & D.C., USA), WCAB (Werner C.A. Bokermann Cruz, 1988, P. luteolus (Wied-Neuwied, 1824), P. Collection, currently in the MZUSP), and referred melanomystax Caramaschi, Silva & Britto-Pereira, in Caramaschi etal (2004) and Peixoto etal (2003). 1992, P. punctatus Caramaschi & Peixoto, 2004, P. Abbreviations used in the accounts below are: SVL tuberculosus Bokermann, 1966, and P. wuchereri (snout-vent length), HL (head length), HW (head width), (Peters, 1873), occurs in eastern Brazil (Caramaschi IND (internarial distance), END (eye-nostril distance), et al, 2004; Frost, 2006). ED (eye diameter), IOD (interorbital distance), TD Herein, we describe a new species of Phyllodytes (tympanum diameter), HDL (hand length), THL (thigh from the northeastern of Minas Gerais and length), TL (tibia length), and FL (foot length). All Southern of Bahia. This new species is related to P. measurements are in millimetres. Webbing formula kautskyi notation follows Savage & Heyer (1967, 1997). 1 Submitted on May 16, 2006. Accepted on October 9, 2006. 2 Museu Nacional/UFRJ, Departamento de Vertebrados, Quinta da Boa Vista, São Cristóvão, 20940-040 Rio de Janeiro, RJ, Brasil. Fellow of Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). 3 E-mail: [email protected]. 4 Universidade Federal de Viçosa, Museu de Zoologia João Moojen de Oliveira, 36571-000, Viçosa, MG, Brasil. E-mail: [email protected]. 5 E-mail: [email protected]. 322 C.A.G.CRUZ, R.N.FEIO & M.C.S.CARDOSO RESULTS kautskyi, P. luteolus, and P. melanomystax (all have a immaculate dorsal color). The remaining species Phyllodytes maculosus sp.nov. of the genus show some different kind of dorsal (Figs.1-2) pattern: P. auratus and P. wuchereri have longitudinal stripes; P. gyrinaethes has variable Holotype - BRAZIL: MINAS GERAIS: Municipality marbled dorsal pattern; P. punctatus and P. of Bandeira (15°49’S, 40°30’W; 837m above sea tuberculosus present distinctive brown dots. levei), MNRJ 41669, adult d , F.R.Melo, F.S.Lima, Phyllodytes maculosus sp.nov. is close to P. kautskyi and M.B.Faria, 15/III/2003. by the size (SVL 39.7-43.5mm in males, 48.5mm in female of the new species and SVL 38.0-42.0mm Paratypes - BRAZIL: MINAS GERAIS: Municipality in males, 43.5mm in female of P. kautskyi). The of Bandeira, MNRJ 41670, adult9 , F.R.Melo, 28/ other species are smaller (SVL in males ranging XII/2004. BAHIA: Municipality of Mascote, Fazenda from 18.2mm in P. punctatus to 35.0mm in P. São José (15°34’S, 39°17W), MNRJ 40251, adult auratus). The rounded snout in dorsal view d , B.V.S.Pimenta and R.V.Lopes, 25-28/XI/2005. separates P. maculosus sp.nov. from P. acuminatus, Diagnosis - A large species (SVL 39.7-43.5mm in P. auratus, P. luteolus, and P. wuchereri (acuminate males, 48.5mm in female) characterized by: (1) snout). Moreover, in profile, the vertical snout dorsum of body, arms, and legs pale brown with distinguishes P. maculosus sp.nov. from P. irregular distinctive cream stains; (2) a dark brown acuminatus, P. auratus, P. brevirostris, P. edelmoi, stripe extending on the eyes, tympanum, and P. gyrinaethes, P. luteolus, P. tuberculosus, and P. reaching the inguinal region; (3) snout short, wuchereri (snout acute or protruding). However, P. rounded in dorsal view, vertical in profile, with a kautskyi, P. melanomystax, and P. punctatus also small apical tubercle; (4) a slight groove extending present vertical snout in profile. Phyllodytes from the nostril to the tympanum and under the maculosus sp.nov., P. acuminatus, P. gyrinaethes, eye; (5) two anterior large and six small odontoids P. luteolus, P. tuberculosus, and P. wuchereri present on each side of the mandible; (6) dorsal skin rugose. on each side of the mandible two large anterior Comparisons with other species - The dorsal color odontoids differing from P. auratus, P. edelmoi, P. pattern (pale brown with irregular distinctive cream kautskyi, and P. punctatus (one larger odontoid), stains) distinguishes Phyllodytes maculosus sp.nov. whereas P. brevirostris and P. melanomystax have from P. acuminatus, P. brevirostris, P. edelmoi, P. no large odontoid. Fig.l- Phyllodytes maculosus sp.nov. (holotype, MNRJ 41669, SVL 21.6mm). (A) Dorsal and (B) ventral views. Arq. Mus. Nac., Rio de Janeiro, v.64, n.4, p.321-324, out./dez.2006 REDESCRIPTION OF A NEW SPECIES OF PHYLLODYTES FROM THE ATLANTIC RAIN FOREST OF MG AND BA, BRAZIL 323 Description of holotype - Body robust; head wider Forearms and arms robust; hand large (Fig.2C), than long; snout short, rounded in dorsal view, 30.3% of SVL; fingers, in order of length, I<II<IV<III; with a small apical tubercle, and vertical in profile round subarticular tubercles present, two-lobed in (Fig.2A-B); nostril on the tip of the snout, elliptical, the second segment of finger IV; few small directed anterolaterally; canthus rostralis poorly supranumerary tubercles; palmar tubercle well defined; loreal region slightly vertical, not concave; developed, ovoid; thenar tubercle large, elliptical; eyes directed anterolaterally; eye diameter 36.7% finger discs as large as tympanum; webbing of head length; interorbital space flat, 34.5% of formula I - II 1,5 - 3 III 2,5 - 2+IV; fingers extensively head width; tympanum distinct, rounded, fringed in both sides. diameter of tympanum 51.8% of eye diameter; Legs robust; tibia slightly longer than thigh; sum supratympanic fold well marked, covering the of thigh and tibia lengths 91.4% of SVL. Foot upper margin of the tympanum; vocal sac (Fig.2D) with distinct round subarticular tubercles subgular, poorly developed; tongue large, rounded, and numerous round supranumerary tubercles; fixed behind; two groups of vomerine teeth on a inner metatarsal tubercle ovoid, spatulate, straight line behind the choanae; each side of the projecting laterally; outer metatarsal tubercle mandible with two large anterior odontoids, the small, rounded; toes, in order of length, first slightly larger, followed by a series of six small I<II<V<III<IV; toe discs slightly smaller than finger others; pupil horizontal, iris projecting an discs; webbing formula 12-3 II 1,5 - 3 III 1,5-3 inconspicuous meniscus on pupil. IV 2.5- 1,5 V. Fig.2- Phyllodytes maculosus sp.nov. (holotype, MNRJ 41669). (A) Dorsal and (B) lateral view of head; ventral views of (C) hand and (D) foot. Arq. Mus. Nac., Rio de Janeiro, v.64, n.4, p.321-324, out./dez.2006 324 C.A.G.CRUZ, R.N.FEIO & M.C.S.CARDOSO Dorsal surface of body, forearms, and tibiae rugose; ACKNOWLEDGEMENTS a slight groove extending from the nostril to the tympanum, passing under the eye; venter We thank W. Ronald Heyer (USNM) and Hussam granulated without distinct series of tubercles on Zaher (MZUSP) for allowing us to examine specimens belly; ventral surface of thighs with several distinct under their care; Ulisses Caramaschi (MNRJ) for tubercles dispersed at thigh insertion. A row of critically reviewing the manuscript; Paulo Roberto distinct tubercles on the outer margins of forearms Nascimento (MNRJ) made the line drawings of the and tarsus. holotype; Fabiano R. Melo (Universidade do Estado de Minas Gerais/Carangola) and Bruno V.S. Pimenta Color in preservative - Dorsum, ílanks, and dorsal (MNRJ) collected the type specimens; Conselho surface of limbs pale brown with irregular Nacional de Desenvolvimento Científico e Tecnológico distinctive cream stains; laterally, a dark brown (CNPq) for financial support to CAGC and MCSC stripe extending on the eye, tympanum, and fellowship. Specimen MNRJ 40251 was collected reaching the inguinal region. Ventral body and during field surveys of the project “Corredor de limbs surfaces cream; palmar and plantar surfaces Biodiversidade da Costa do Cacau” supported by the brownish. Criticai Ecosystem Partnership Fund (CEPF) and the Color in life - Dorsum of body greenish cream with United States Agency for International Development pale brown stains; snout and limbs light-green; (USAID), coordinated by the Instituto de Estudos laterally, a dark brown stripe Crossing the eye, Sócio-Ambientais do Sul da Bahia (IESB). This work tympanum, and reaching the inguinal region; is part of the Project “Inventário de áreas prioritárias venter white; iris copper. para conservação da biodiversidade nos vales dos rios Jequitinhonha e Mucuri”, supported by the Project Variation - The type specimens present little on the Conservation and Sustainable Use of Brazilian variation in morphological aspects and color pattern. Biological Diversity/Brazilian Ministry of the The dorsal background color varies from tan to light Environment (PROBIO/MMA), coordinated by the grey. The dorsal pattern is not well defined in the Conservation International do Brasil (CI-Brasil). specimen MNRJ 40251. Measurements of the type series are presented in table 1. REFERENCES Etymology - The specific name refers to distinctive dorsal color pattern. Maculosus is a latin vernacular CARAMASCHI, U.; PEIXOTO, O.L. & RODRIGUES, M.T., name meaning “covered with stains”. 2004. Revalidation and redescription of Phyllodytes wuchereri (Peters, 1873) (Amphibia, Anura, Hylidae). Remarks - The geographical distribution of Arquivos do Museu Nacional, 62(2): 185-191. Phyllodytes kautskyi referred by Peixoto & Caramaschi (2002) to the State of Bahia, Brazil, is FROST, D.R., 2006. Amphibian Species of the World: actually related to P. maculosus sp.nov. (MZUSP an Online Reference. Version 3.0 (17 August 2006). 63672,63673). Electronic Database available at: <http:// research.amnh.org/herpetology/amphibia/index>. American Museum of Natural History, New York, USA. TABLE 1. Measurements in millimetres of the specimens of Acessed on: 6 Sept. 2006. the type-series of Phyllodytes maculosus sp.nov. PEIXOTO, O.L. & CARAMASCHI, U., 2002. Phyllodytes MNRJ 41669 MNRJ 40251 MNRJ 41670 kautskyi (NCN). Herpetological Review, 33(4):318. d, holotype d, paratype 9 , paratype SVL 44.2 40.2 49.5 PEIXOTO, O.L., CARAMASCHI, U. & FREIRE, E.M.X., HL 14.7 13.6 15.5 2003. Two new species of Phyllodytes (Anura: Hylidae) HW 17.4 15.8 18.6 from the State of Alagoas, northeastern Brazil. IND 2.1 2.1 2.6 Herpetologica, 59(l):235-246. END 4.8 4.3 4.9 SAVAGE, J.M. & HEYER, W.R., 1967. Variation and ED 5.4 4.5 5.5 distribution in the tree-frog genus Phyllomedusain Costa IOD 6.0 6.1 6.4 Rica, Central America. Beitràge zur Neotropischen TD 2.8 2.9 2.7 Fauna, 5(2): 111-131. HDL 13.4 12.0 13.7 THL 19.7 19.1 21.3 SAVAGE, J.M. & HEYER, W.R., 1997. Digital webbing TL 20.7 20.8 22.1 formulae for anurans: a refinement. Herpetological FL 27.0 26.8 29.0 Review, 28(3): 131. Arq. Mus. Nac., Rio de Janeiro, v.64, n.4, p.321-324, out./dez.2006