Conférence nationale GdR Phycotox & GIS Cyano 2017 Résumés des présentations et des posters 14 - 16 Mars 2017, Gif-sur-Yvette (France) GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette Comité scientifique d’organisation Romulo ARAOZ ......................................... CNRS – Gif-sur-Yvette Hélène HEGARET ...................................... IUEM – LEMAR - Brest Philipp HESS ............................................. IFREMER – Nantes DYNECO/PHYC Christophe LAPLACE-TREYTURE ........... IRSTEA - Bordeaux Delphine LATOUR ..................................... UNIVERSITE de Clermont-Ferrand Benjamin MARIE ....................................... MNHN – Paris Joël ROBIN ................................................ ISARA – Lyon Raffaele SIANO .......................................... IFREMER – Brest DYNECO/Pelagos Anne THEBAULT ....................................... ANSES – Maisons-Alfort. Remerciements: Nous souhaitons remercier le CNRS de Gif-sur-Yvette d’avoir accueilli cette conférence dans ses locaux. Que soit aussi remercié le CNRS, Irstea, l’Ifremer, l’ANSES et les sociétés Novakits et Bionef pour leur soutien financier. Enfin, nous remercions toutes les personnes qui se sont impliquées de près ou de loin dans l’organisation de cette conférence et toutes celles qui apporteront leur aide précieuse pour en assurer le bon déroulement le jour J. Couverture : schéma réalisé par Romulo Araoz (CNRS – Gif/Yvette), mise en page par Françoise Peyriguer (Irstea – Antony). GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette Program Tuesday 14 March 2017 13:30-14:00 Welcome coffee and inscription 14:00-14:15 Introductory speech 14:15-15:00 Invited conference: The Integrated Pest Management Model as a Framework for Aquaculture HAB Mitigation Gary Wikfors 15:00-16:00 THEME: Effects of HABs on professional sectors and society at large: from legal and socio-economic perspectives Chairmans: Christophe Laplace-Treyture & Rodolphe Lemée 15:00-15:15 Coordinating the implementation of the interagency Global CFP Strategy Mireille Chinain, Marie-Yasmine Dechraoui Bottein, Philipp Hess 15:15-15:30 Pinnatoxins, an emergent class of fast-acting marine toxins, are potential threat for neurodevelopment and global health Sophie Creuzet, Aurélie Couesnon, Vincent Dive, Armen Zakarian, Jordi Molgó, Denis Servant 15:30-15:45 Eaters of oyster: From the risk of contamination by toxic algae to social representations and practices of consumption Elisabeth Guillou, Adeline Raymond 15:45-16:00 Thinking out of the box: finding new ways of regulating marine toxins Marie Bonnin, Hélène Hégaret, Philippe Soudant, Philippe Fotso, Caroline Fabioux, Philipp Hess 16:00-16:30 Coffee break 16:30-18:15 THEME: Tropics Chairmans: Christophe Laplace-Treyture & Rodolphe Lemée 16:30-16:45 Ciguatera incidence in the Caribbean Islands Jose Ernesto Mancera 16:45-17:00 Microalgae from French Polynesian lagoons: novel source of bioactive compounds? Mireille Chinain, Sebastien Longo, Kevin Henry, André Ung, Taiana Darius, Jérôme Viallon, Martine Rodier, Bruno Delesalle, Alain Lo-Yat, Gilles Le Moullac, Romulo Araoz 17:00-17:15 Distribution and abundances of benthic toxic dinoflagellates in French West Indies Aurélie Boisnoir, Pierre-Yves Pascal, Sophie Marro, Rodolphe Lemée 17:15-17:30 Taxonomic diversity of the dinoflagellate genus Ostreopsis in French Polynesia: preliminary results from Marquesas Islands (Pacific Ocean) Nicolas Chomérat, Clémence Gatti, Gwenael Bilien, André Ung, H. Taiana Darius, R. Wayne Litaker, Patricia Tester, Mireille Chinain 17:30-17:45 Taxonomic characterization of Gambierdiscus species in Reunion Island, Indian Ocean Alina Tunin-Ley, Nicolas Chomérat, Gwenael Bilien, Jean Turquet 1 GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette 17:45 18:00 Environmental control and dynamics of blooms of the benthic toxic dinoflagellate Ostreopsis cf. ovata in the Mediterranean Sea Cécile Jauzein, Rodolphe Lemée 18h00 18h15 Ostreopsis blooms at the global scale: influence of macroalgal communities Luisa Passeron Mangialajo, Rodolphe Lemée, Anna Fricke, Daniela Catania, Sarah Kulins, Cécile Jauzein, Nick Shears 18:15-19:00 GdR Phycotox Annual general meeting 19:00-20:00 Welcome cocktail Wednesday 15 March 2017 09:00-09:45 Invited conference: The diatom genus Pseudo-nitzschia in the Gulf of Naples (Mediterranean Sea): exploring diversity and life history traits Marina Montresor 09:45-10:45 THEME: Ecology of HAB Chairmans: Delphine Latour & Mathilde Schapira 09:45-10:00 Genetic, morphological and ecological evidence of new oomycete parasites of the toxic diatom genus Pseudo-nitzschia Garvetto Andrea, Claire Gachon, Elisabeth Nézan, Raffaele Siano 10:00-10:15 Effects of salinity variation on the physiology and domoic acid production by two strains of Pseudo-nitzschia australis Nour Ayache, Amandine Caruana, Fabienne Hervé, Zouher Amzil 10:15-10:30 Interspecific and intraspecific variability in physiology among four Pseudo-nitzschia species Aurore Sauvey, Pascal Claquin, Juliette Fauchot 10:30-10:45 Nutrient ratios influence variability in Pseudo-nitzschia species diversity and particulate domoic acid production in the Bay of Seine (France) Juliette Fauchot, Maxine Thorel, Mathilde Schapira, Romain Le Gendre, Philippe Riou, Didier Goux, Bertrand Le Roy, Virginie Raimbault, Anne-Flore Deton-Cabanillas, Pauline Bazin, Valérie Kientz-Bouchart, Pascal Claquin 10:45-11:15 Coffee break 11:15-12:30 THEME: Diversity and theoretical ecological niches of HAB, synthesis and transformation of metabolites, analytical challenges Chairmans: Delphine Latour & Mathilde Schapira 11:15-11:30 Benthic Planktothrix and their antifungal natural products Claire Pancrace, Jouni Jokela, Nathalie Sassoon, Christelle Ganneau, Marie Desnos- Ollivier, Matti Wahlsten, Anu Humisto, Alexandra Calteau, Sylvie Bay, David P. Fewer, Kaarina Sivonen, Muriel Gugger 11:30-11:45 From cyanobacteria to mammals: role of ergothionein, glutathione and its analogs in stressors signalling and detoxication Kinsley Narainsamy, Christophe Junot, Corinne Cassier-Chauvat, Franck Chauvat 2 GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette 11:45-12:00 Latest advances in two functional methods to detect ciguatoxins (CTXs): the Receptor Binding Assay (RBA) & the Cell Based Assay (CBA-N2a) Taiana Darius, Jérôme Viallon, Jennifer Mc Call, Taina Revel, Ransom Hardison, William Holland, Patricia Tester, Wayne Litaker, Mireille Chinain 12:15-12:30 Rapid characterization of microcystin-producing cyanobacteria in freshwater lakes by TSA-FISH (Tyramid Signal Amplification - Fluorescent In Situ Hybridization) Isabelle Biegala, Luc Brient 12:30-14:00 Lunch 14:00-:14:45 Invited conference: The Užice Case Jussy Meriluoto, Zorica Svirčev 14:45-16:15 THEME: Human and animal toxicology Chairmans: Benjamin Marie & Ronel Biré 14:45-15:00 Mixtures of lipophilic phycotoxins: exposure data and in vitro toxicity assessment Jimmy Alarcan, Ronel Biré, Ludovic Le Hegarat, Valérie Fessard 15:00-15:15 Ostreopsis cf. ovata blooms in the NW Mediterranean: Exploring the links between toxins variability and impacts on human health Elisa Berdalet, Rafael Abós-Herràndiz, Marta Estrada 15:15-15:30 Maitotoxin-4, a novel MTX analog produced by Gambierdiscus excentricus Francesco Pisapia, Manoëlla Sibat, Christine Herrenknecht, Korian Lhaute, Greta Gaiani, Pierre-Jean Ferron, Valérie Fessard, Catherine Roullier, Santiago Fraga, Silvia Nascimento, Wayne Litaker, Philipp Hess 15:30-15:45 Transgenerational effects of Microcystin-LR on Daphnia magna Claudia Wiegand, Rafael Ortiz-Rodríguez, Thanh Dao 15:45-16:00 Gambierol enhances quantal acetylcholine release from motor nerve terminals of vertebrate neuromuscular junctions Jordi Molgó, Sébastien Schlumberger, Makoto Sasaki, Haruhiko Fuwa, M. Louzao, Luis Botana, Evelyne Benoit 16:00-16:15 Receptor-based lateral flow test for fast and early detection of neurotoxins acting on nicotinic acetylcholine receptors Romulo Araoz, Denis Servent 16:15-16:45 Coffee break 16:45-17:15 POSTER SESSION: My poster in 3 minutes Chairman: Valérie Fessard 17:15-19:30 POSTER SESSION 18:30-19 :30 Réunion du comité de pilotage pour préparer la conférence ICHA 2018 à Nantes 19:30 Diner cocktail 3 GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette Thursday 16 March 2017 09:00-09:45 Invited conference: Differential Effects of Algal Toxins on Grazing by the Marine Copepod Acartia tonsa Patricia Tester, Jiarong Hong, Rebecca Waggett, Joseph Katz, Allen Place, Erik Davenport 09:45-10:45 THEME: Effects of HAB on marine organisms and ecosystems, trophic transfer, etc. Chairmans: Juliette Fauchot & Cécile Bernard 09:45-10:00 Interest of bivalves for the biosurvey of environmental contamination by cyanobacterial toxins Emilie Lance, Zouher Amzil, Alexandra Lepoutre, Luc Brient, Myriam Bormans 10:00-10:15 Impacts of Alexandrium minutum on the reproduction of the oyster Crassostrea gigas: from gametogenesis to larval settlement Justine Castrec, Philippe Soudant, Marianne Alunno Bruscia, Bruno Petton, Isabelle Queau, Marc Suquet, Dominique Ratiskol, Jacqueline Le Grand, Christian Mingant, Nelly Le Goic, Hélène Hégaret, Caroline Fabioux 10:15-10:30 Impact of the environmental toxin BMAA on the immune system of the zebra mussel Dreissena polymorpha. Alexandra Lepoutre, Nadia Milliote, Mélissa Palos Ladeiro, Marc Bonnard, Elisabeth Faassen, Alain Geffard, Emilie Lance 10:30-10:45 Metabolomics for cyanobacteria ecotoxicology: insights from the Toxcyfish project Benjamin Marie, Benoit Sotton, Séverine Le Manach, Alain Paris 10:45-11:15 Coffee break 11:15-11:30 Short term effects of the allelochemicals released by the toxic dinoflagellate Alexandrium minutum upon the diatom Chaetoceros sp. Marc Long, Kevin Tallec, Philippe Soudant, Fabienne Le Grand, Géraldine Sarthou, Dianne Jolley, Hélène Hégaret 11:30-11:45 Phenolic compounds from the seagrasses Zostera marina and Zostera noltei affect negatively the physiology of the neurotoxic dinoflagellate Alexandrium catenella Mohamed Laabir 11:45-12:00 Paralytic Shellfish Toxin accumulation in oyster is predicted by gene expression Audrey Mat, Christophe Klopp, Laura Payton, Céline Jeziorski, Morgane Chalopin, Zouher Amzil, Damien Tran, Hélène Hégaret, Philippe Soudant, Caroline Fabioux, Arnaud Huvet 12:00-12:15 The Paralytic Shellfish Toxin, Saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells via a caspases-dependent pathway Jean-Luc Rolland, Célina Abi-Khalil, Darren Finkelstein, Genevieve Conejero, Justin Du Bois, Delphine Destoumieux-Garzon 12:15-12:30 Continued feeding with Gambierdiscus polynesiensis causes increasing accumulation of ciguatoxin over 16 weeks in an herbivorous reef fish Rachel Clausing, Barbara Losen, Taiana Darius, Philipp Hess, Manoella Sibat, Jerome Viallon, Mireille Chinain, Marie-Yasmine Dechraoui Bottein 12:30-12:45 Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea 4 GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette gigas Floriane Boullot, Justine Castrec, Adeline Bidault-Toffin, Natanael Dantas, Laura Payton, Mickael Perrigault, Damien Tran, Zouher Amzil, Pierre BOUDRY, Philippe Soudant, Hélène Hegaret, Caroline Fabioux 12:45-14:15 Lunch 14:15-15:00 Invited conference: Strategies to reduce toxins in shellfish Ana Garcia Cabado 15:00-16:15 THEME: Diversity and theoretical ecological niches of HAB, synthesis and transformation of metabolites, analytical challenges Chairmans: Joël Robin & Elisa Berdalet 15:00-15:15 Monitoring and modelling cyanobacteria biomass in a small urban lake Denis Plec, Lucas Jardim Porto, Bruno Lemaire, Philippe Dubois, Mohamed Saad, Cécile Bernard, Laure Huguenard, Brigitte Vinçon-Leite 15:15-15:30 Temperature and stratification of the artificial lake and the proliferation of cyanobacteria, case of the lake of the Eguzon dam - France Zoubida Qsair, Laurent Touchart, Pascal Bartout 15:30-15:45 Genetic diversity of the toxic bloom-forming cyanobacterium Microcystis aeruginosa: a meta-analysis at large spatial and temporal scales Marion Sabart, Benjamin Misson, Marlène Jobard, Gaofei Song, Li Renhui, Delphine Latour 15:45-16:00 Wise use of metabolomics in the field of marine chemical ecology Eva Ternon, Anne-Sophie Pavaux, Olivier Thomas 16:00-16:15 Metabolomics of ancient populations of DINoflAgellates in phosphorous-limited conditions Raffaele Siano, Florence Mondeguer, Marie Latimier, Julien Quéré, Manoella Sibat, Laure Guillou, Philipp Hess 16:15 End of the symposium 5 GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette Table of contents Theme 1: Diversity and theoretical ecological niches of HAB, synthesis and transformation of metabolites, analytical challenges ..........................................................12 Rapid characterization of microcystin-producing cyanobacteria in freshwater lakes by TSA- FISH (Tyramid Signal Amplification - Fluorescent In Situ Hybridization), Isabelle Biegala [et al.] ......................................................................................................13 Latest advances in two functional methods to detect ciguatoxins (CTXs): the Receptor Binding Assay (RBA) & the Cell Based Assay (CBA-N2a), Taiana Darius [et al.] ........................................................................................................14 From cyanobacteria to mammals: role of ergothionein, glutathione and its analogs in stressors signalling and detoxication, Kinsley Narainsamy [et al.]. ..............................................................................................15 Benthic Planktothrix and their antifungal natural products, Claire Pancrace [et al.] ......................................................................................................16 Monitoring and modelling cyanobacteria biomass in a small urban lake, Denis Plec [et al.]. ............................................................................................................17 Temperature and stratification of the artificial lake and the proliferation of cyanobacteria, case of the lake of the Eguzon dam - France, Zoubida Qsair [et al.] ........................................................................................................18 Genetic diversity of the toxic bloom-forming cyanobacterium Microcystis aeruginosa: a meta-analysis at large spatial and temporal scales, Marion Sabart [et al.]. ......................................................................................................19 MEtabolomics of ancient populations of DINoflAgellates in phosphorous-limited conditions, Raffaele Siano [et al.] ........................................................................................................20 Wise use of metabolomics in the field of marine chemical ecology, Eva Ternon [et al.]. ...........................................................................................................21 Theme 2: Ecology of HAB ....................................................................................................22 Genetic, morphological and ecological evidence of new oomycete parasites of the toxic diatom genus Pseudo-nitzschia, Garvetto Andrea [et al.] ....................................................................................................23 Effects of salinity variation on the physiology and domoic acid production by two strains of Pseudo-nitzschia australis, Nour Ayache [et al.] ..........................................................................................................24 Nutrient ratios influence variability in Pseudo-nitzschia species diversity and particulate domoic acid production in the Bay of Seine (France), Juliette Fauchot [et al.] .....................................................................................................25 6 GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette The diatom genus Pseudo-nitzschia in the Gulf of Naples (Mediterranean Sea): exploring diversity and life history traits, Marina Montresor ........................................................................................................................ 26 Interspecific and intraspecific variability in physiology among four Pseudo-nitzschia species, Aurore Sauvey [et al.] ........................................................................... 27 Theme 3: Effects of HAB on marine organisms and ecosystems, trophic transfer, etc. 28 Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas, Floriane Boullot [et al.] .....................................................................................................29 Impacts of Alexandrium minutum on the reproduction of the oyster Crassostrea gigas: from gametogenesis to larval settlement, Justine Castrec [et al.] ......................................................................................................30 Continued feeding with Gambierdiscus polynesiensis causes increasing accumulation of ciguatoxin over 16 weeks in an herbivorous reef fish, Rachel Clausing [et al.]. ....................................................................................................31 Phenolic compounds from the seagrasses Zostera marina and Zostera noltei affect negatively the physiology of the neurotoxic dinoflagellate Alexandrium catenella, Mohamed Laabir ...............................................................................................................32 Interest of bivalves for the biosurvey of environmental contamination by cyanobacterial toxins, Emilie Lance [et al.] ..........................................................................................................33 Impact of the environmental toxin BMAA on the immune system of the zebra mussel Dreissena polymorpha, Alexandra Lepoutre [et al.] ...............................................................................................34 Short term effects of the allelochemicals released by the toxic dinoflagellate Alexandrium minutum upon the diatom Chaetoceros sp.. Marc Long [et al.] .............................................................................................................35 Metabolomics for cyanobacteria ecotoxicology: insights from the Toxcyfish project, Benjamin Marie [et al.] .....................................................................................................36 Paralytic Shellfish Toxin accumulation in oyster is predicted by gene expression, Audrey Mat [et al.]. ..........................................................................................................37 The Paralytic Shellfish Toxin, Saxitoxin, enters the cytoplasm and induces apoptosis of oyster immune cells via a caspases-dependent pathway, Jean-Luc Rolland [et al.] ..................................................................................................38 Differential Effects of Algal Toxins on Grazing by the Marine Copepod Acartia tonsa, Patricia Tester [et al.] .......................................................................................................39 7 GdR Phycotox – GIS Cyano 2017, Gif-sur-Yvette Theme 4: Human and animal toxicology ..............................................................................40 Receptor-based lateral flow test for fast and early detection of neurotoxins acting on nicotinic acetylcholine receptors., Romulo Araoz [et al.]........................................................................................................41 Mixtures of lipophilic phycotoxins: exposure data and in vitro toxicity assessment, Jimmy Alarcan [et al.] ......................................................................................................42 Ostreopsis cf. ovata blooms in the NW Mediterranean: Exploring the links between toxins variability and impacts on human health, Elisa Berdalet [et al.] ........................................................................................................43 The Užice Case, Jussi Meriluoto [et al.] .......................................................................................................................... 44 Gambierol enhances quantal acetylcholine release from motor nerve terminals of vertebrate neuromuscular junctions, Jordi Molg´o [et al.] ...........................................................................................................45 Maitotoxin-4, a novel MTX analog produced by Gambierdiscus excentricus, Francesco Pisapia [et al.]. ................................................................................................46 Transgenerational effects of Microcystin-LR on Daphnia magna, Claudia Wiegand [et al.]. ..................................................................................................47 Theme 5: Effects of HABs on professional sectors and society at large: from legal and socio-economic perspectives ................................................................................................ 48 Thinking out of the box: finding news ways of regulating marine toxins, Marie Bonnin [et al.] .........................................................................................................49 Coordinating the implementation of the interagency Global CFP Strategy, Mireille Chinain [et al.]. ....................................................................................................50 Pinnatoxins, an emergent class of fast-acting marine toxins, are potential threat for neurodevelopment and global health, Sophie Creuzet [et al.]. ......................................................................................................51 Strategies to reduce toxins in shellfish, Ana Garcia-Cabado .........................................................................................................52 Eaters of oyster: From the risk of contamination by toxic algae to social representations and practices of consumption, Elisabeth Guillou [et al.] ...................................................................................................53 The Integrated Pest Management Model as a Framework for Aquaculture HAB Mitigation, Gary Wikfors ....................................................................................................................54 8