Challenges in co-extraction of marine algal active ingredients: An exemple of co-extraction of alginates and fucoidans Franck Hennequart, Director Research & Innovation, ALGAIA St Lô Algaia™ portfolio Red Seaweed Extracts Brown Seaweed Extracts Tailor made solutions Carrageenans Alginates Customized Solutions Sold under Carra™ trade name Sold under Satialgine™, AlgoGel™ and Cecalgum™ Sold under Algogel™ tradename Produced in Chile at our strategic partner Gelymar Produced at Algaia’sFrench factory in Lannilis Produced in France R&D services with partners Specialty Seaweed extracts AlgaiaServices Under Development Biomass, analytical, bioactivity etc… with specific partners or through JDA Provided from our R&D center in Saint Lô(France) To be produced at Algaia’sFrench facility in Lannilis 2 Algaia™ at a glance Production facility at Lannilis Next to Brest ▪ 80,000 m2 land space – 15,000 m2 occupied ▪ Over 40 years experience in Alginate production ▪ ISO 9001 since 1994 ▪ FSSC 22000 certification R&D center at Saint Lô Next to Caen Lannilis ▪ 1000 m2 state-of-the-art R&D facility ✓ Extraction laboratory ✓ Analytical laboratory ✓ Application laboratory ✓ Pilot room (in development) Headquarter in Paris Strategic Alliance with Gelymar Located in Chile Paris ▪ 4th largest Carrageenan producer globally ▪ ISO 9001 Saint Lô ▪ FSSC 22000 certification ▪ Pharma cGMP production line 3 Algaia™ at a glance Laminaria digitata ➢ France, 2nd processor country of seaweed in volumes in Europe (about 80,000 Ton/year) ➢ Algaia: 40,000 Tons of fresh seaweed/ year Laminaria hyperborea 4 Algaia™ at a glance Innovative Natural Upon Cargill’s Ingredients acquisition : Alginates Green . Satialgine™ range globally Specialties Biorefinery . Algogel™ range AlgogelCustomized™ Brand Name . Cecalgum™ range Functional Systems Carrageenans in Europe Red Seaweed & other natural extracts Carra™ Brand Name Brown Seaweed Algaia’s Services Biomass Potential Analytical R&D services Enhance functionalities 5 Bioactivity Process development Industries we serve… 6 ALGAIA Services – AAnnaallyysseess & Bioactivities Saccharides Fatty acid profile • Total sugars • Omega 6 • Identification and quantification of polysaccharides • Omega 3 • Characterisation in the composition • Phospholipids • Study of the size range • … Antioxidant compounds • Phenolic compounds Genomic identification • Vitamins • Microalgae strains • Pigments • Seaweeds Proteins • …. • Total Proteins • Amino Acids Ionic Profile • Anions Pigment profile • Cations • Chlorophylls • Heavy metals • Fucoxanthin • Isotopic ICPMS • Astaxanthin • Phycocyanin • …. 7 ALGAIA Services – Analyses & BBiiooaaccttiivviittiieess Prebiotic Anti-microbial • Resistance to gastric enzymes • Antiviral • Selective fermentation of intestinal bacteria strains • Antibacterial • Antifungal Anti-cholesterol • Influence on activity of selected genes involved in cholesterol absorption • Influence on activity of selected genes involved in cholesterol refining • Intestinal Absorption of cholestérol in vitro Anti-cancer Anti-inflammatory • Influence on selected genes involved in apoptosis • Enzymatic assays • Proliferation • Cytokines, interleukines • Adhesion • …. • Migration and invasion • High flow screening Cardiovascular Cosmetic Plant biostimulation • Atherosclerosis • Anti-ageing • Germination • Heart failure • Anti-UV • Defense elicitors • hypertension • Antioxidant • Plant nutrition / Stress • Obesity • Depigmentation • Plant defense gene expression • Diabetes • Cicatrisation 8 ALGAIA Services – boost R&D and markets Culture/Wild stock of Macro & Users of algal extracts Producers of algal extracts Micro algae Active formulae • Analyses of batch composition • Analyses of batch composition • Studies on compatibility of in production in extract production extracts in formulated products • Study on batch variability • Study on batch variability • Studies on products stability • Statistic studies on influence of • Statistic studies on influence of based on algal extracts key cultivation parameters key production parameters • Test on bioactivities of • Study on the valorisation of the • Tests on extract bioactivities formulated products production • Valorisation of by-products • Identification of active algal • Support on potential markets of extracts interest • Development of novel algal • Support on wild biomass extracts assessment and management • Support in cultivation system setup Get into sustainable commercial development as soon as possible ! 9 Biorefinery / Cracking ALGAIA – First Biorefinery studies Lessonia nigrescens Lessonia trabeculata Macrocystis pyrifera Oligo-elements Proteins Dietary Fibers Alginates Fucoidans Pigments Lipids Laminarans Polyphenols Iodine 10