student Research Forum Centenary College of Louisiana Student Research Forum April 22&23,2010 Posters Mickle Hall (2nd floor) 11:00 A.M. April 22,2010 Natural Sciences Carlile Auditorium (Mickle Hall first floor) 12:55-3:30 P.M. April 23,2010 Social Sciences Kilpatrick Auditorium (Smith Building) 1:25-3:00 P.M. April 23,2010 Humanities Hurley Music Building, Rm. 201 1:55-3:10 PM. April 23,2010 CENTENARY COLLEGE I9TH ANNUAL STUDENT RESEARCH FORUM •v JnobuJJi '■4^ «s *■ » rfousesH mino? orts^stiriiqA e«rteoS RtHal^ (wOhfiii * KUOttll ' ! 1 OfOtiiLiq»i:-j|.: 2d0fidiD8 inulGlI nriutMiiitAoMhtO (vioQ t?iB tkH ibbild ' HH(»£ m.nifi^'' 26s)tt0i98 lebo8 (ii^Hiii/i riAof^ *l; '.- V MUimuntfR . Ii(.riH jtniMMviaUyiWl ' ’ J*I n. : »■>= .. K'^'f Centenary College of Louisiana Student Research Forum Sponsored by Centenary Book Bazaar AND THE Provost and Dean of the College Judges Dr. David Bieler. . Associate Professor of Geology Dr. Greg Butcher. .... Assistant Professor of Neuroscience Dr. Scott Chirhart. . Associate Professor of Biology Dr. Christopher Ciocchetti . Associate Professor of Philosophy Dr. Daniel Henderson. .Assistant Professor of Exercise Science Dr. Robert Prickett. . Assistant Professor of Education Dr. Helen Sikes. J^rofessor of Accounting and Einance Dr. Ross Smith. . Associate Professor of Music From the Director The annual Student Research Forum provides Centenary students an opportunity to showcase their talents and experiences. They are to be commended for their efforts and quality work. Thank you research participants and advisers for doing the projects and presenting your work in this forum. Judges, thank you for your time and effort. Special thanks is expressed to the Centenary Book Bazaar for their financial support. We greatly appreciate it! Sincere thanks is given to the Provost and Dean of Centenary, Dr. Daniel Keck, for all of his financial support and encouragement. Thank you Mrs. Jeannie Clements, Office of the Provost, and Ms. Sherry Heflin, Office of Marketing and Communications, for all of your work on planning the forum, designing/producing the brochure and the T-shirts, room arrangements, refreshments, etc. You are the best and much of our success is credited to you! Dr. Matthew Weeks, Director 19th Annual Student Research Fomm centenary college I9TH ANNUAL STUDENT RESEARCH FORUM 3 Schedule POSTER Presentations/Judging * Presentation and judging will take place on Thursday, April 22nd at 11:10am in Mickle Hall on the second floor. In preparation of the judging, presenters should have their posters in place and ready at their assigned locations no later than 11:00 A.M. Dallas Krentzel.Biochemistry Kelly Reed.Biology Kelly Reed (2).Biology Taylor Pahls.Biology Joshua Phillips.Biology Elise McMahen.Biology Rustin Green.Biology Brittany Bienvenu.Biology Jennifer Flenniken.Biology Ahmad Azzawe.Biology, Environmental Science Marco Rajo.Neuroscience John Cefalu.Neuroscience Sunil Kaimootil.Biophysics Daniel Martin.Health & Exercise Science ORAL Presentations/Judging * Presentation and judging will take place on Friday, April 23rd. Locations and starting time vary by division. Natural Sciences in Carlile Auditorium 12:55 P.M. Provost Daniel Keck. .Introduction/Welcome 1:00 P.M. Kathryn Hardey & Bradlee Robertson.Computer Science 1:10 P.M. Nolan Baker. .Computer Science 1:20 P.M. David Moss. .Geology 1:30 P.M. Amanda Krentzel. .Neuroscience 1:40 P.M. Mark Moehle. .Neuroscience 1:50 P.M. Kathryn Craigo. .Chemistry 2:00 P.M. Matthew Blanchard. .Chemistry 2:10 PM. Rebecca McMahen. .Chemistry 4 CENTENARY COLLEGE I9TH ANNUAL STUDENT RESEARCH FORUM Schedule 2:20 P.M. Ruth Litwinozicz.Biology 2:30 P.M. Jonathan Carrere.Biology 2:40 P.M. Marci McMahen.Biology 2:50 P.M. Brandi Candler.Biochemistry 3:00 P.M. Robert Grand.Biochemistry 3:10 P.M. Everett Grimley.Biophysics 3:20 P.M. John Cefalu.Neuroscience *Natural Sciences Oral Presentations end about 3:30 P.M. * Social Sciences in Kilpatrick Auditorium 1:25 P.M.Provost Daniel Keck.Introduction/Welcome 1:30 P.M. Jessica Marquart.Business 1:40 P.M. Michael Jarboe.Business 1:50 P.M. Stephanie Beauvais.Finance 2:00 P.M. Quinn Larwood.Economics 2:10 P.M. Kenneshea Allums.Psychology 2:20 P.M. Anne Zapczynski.Psychology 2:30 P.M. John Webb.Psychology 2:40 P.M. Nicholas Akins.Sociology 2:50 P.M. Kenneshea Allums.Sociology *Social Science Oral Presentations end about 3:00 P.M. * Humanities in Hurley Music Building, Room 201 1:55 P.M. Provost Daniel Keck.Introduction/Welcome 2:00 P.M. Sarah Savage.History 2:10 P.M. Spencer Bostwick.History 2:20 P.M. Janeane Gorcyca.History 2:30 P.M. Christene Paxton.Religious Studies 2:40 P.M. Marissa Teauseau.Religious Studies 2:50 P.M. Rachel Stevens.Religious Studies 3:00 P.M. Rachel McConnell-Switzer.Religious Studies ‘^Humanities Oral Presentations end about 3:10 P.M. * CENTENARY COLLEGE I9TH ANNUAL STUDENT RESEARCH FORUM 5 Posters Posters Investigation of the Conserved RD Pocket in the CKI PROTEIN KINASE FAMILY ^DALLAS KRENTZEL^, WITH NOOR AZZAWE, MICHAELA BERG, CAITLIN CAVARRA, JOHN CEFALU, JORDAN DAY, ROB GRAND, SARAH KURUVILLA, RUTH LITWINOWITZ, COLIN MCRAE, MARCO RAJO, KELLY REED, TYLER SMITH, GARRETT VICK, HEATHER WENSLER Researcher Advisors: Dr. Cynthia J. Brame^ & Dr. Lucy C. Robinson^ ^Department of Biology Centenary College of Louisiana ^Louisiana State University Health Sciences Center Shreveport, LA Members of the CKI subfamily of protein kinases regulate cell differentiation and proliferation, chromosome segregation, and circadian rhythm. Like all protein kinases, CKls catalyze transferral of phosphate from ATP to a protein substrate. One of the family-specific features of CKI kinases is a long activation loop and an RD pocket, an area of positively charged residues on the surface of the enzyme. In some kinase families, phosphorylation within the activation loop may activate the enzyme via binding of the phosphorylated residue to the RD pocket. We hypothesize that the RD pocket is involved in inhibiting CKI kinases by binding phosphorylated residues from the activation loop. Here we have used a model CKI, YCK2, from the '^&2&{Saccharomyces cerevisiae. Each of the three positively charged residues comprising the RD pocket was mutated to a negatively charged glutamate and a neutral glutamine via site-directed mutagenesis. We predicted that substitution of neutral residues would slightly decrease YCK2 activity while substitution of negative residues will dramatically decrease YCK2 activity. Plasmids containing the mutant alleles were cloned, isolated, and sequenced to confirm introduction of the mutations. The mutant YCK2 alleles were then expressed in yeast. Effect on function was assessed by complementation tests and microscopy. Effects of Various Fertilizers on Earthworm (Lumbricus terrestris) Metabolism and Soil pH KELLY REED Research Advisor: Dr. Beth Leuck Department of Biology Centenary College of Louisiana I compared the effects of fertilizer with high nitrogen content to fertilizer with lower nitrogen content on earthworm CO2 production and soil acidity. I hypothesized that the fertilizer with high nitrogen content would decrease the pH of the soil and that this drop in pH would decrease the earthworms’ metabolic rate. Furthermore, I hypothesized that there would be no difference in earthworm metabolism and soil acidity between the organic fertilizer and the soil with no fertilizer. Earthworms were divided into four groups and were exposed to a high nitrogen content fertilizer, a lower nitrogen content fertilizer, organic fertilizer, or no fertilizer for a period of six days. Soil pH was measured 6 CENTENARY COLLEGE I9TH ANNUAL STUDENT RESEARCH FORUM Posters on day one and day six of the experiment, and three replications were carried out. Although the data was not significantly different, the group exposed to the high nitrogen fertilizer had the lowest CO emission followed by the group 2 exposed to lower nitrogen fertilizer, the group exposed to organic fertilizer, and the control group, which had the greatest CO emission. Soil acidity decreased 2 for each group except those in the organic fertilizer. Thymoquinone(TQ) and Cisplatin(CDDP) IN A Non-small Cell Lung Cancer(NSCLC) Xenograft Model *KELiy REED, SYED H. JAFRI, MD, JONATHAN GLASS, MD, RUNHUA SHI, MD/PHD, SONGLIN ZHANG, MD/PHD, MISTY PRINC, & HEATHER KLEINER-HANCOCK, PHD Louisiana State University Health Sciences Center Shreveport, LA Lung cancer is the most common cause of cancer-related deaths in the United States. Cisplatin is a common chemotherapy drug used in the treatment of non-small cell lung cancer (NSCLS). Thymoquinone (TQ) is a naturally occurring antioxidant found in \he.Nigella sativus plant; there is evidence to suggest that TQ has various anti-cancer effects. In this study, thymoquinone was used in combination with cisplatin in a mouse xenograft model using a NSCLC cell line NCI-H460. The mice were divided into six groups each receiving subcutaneous injections of 5mg/kg of TQ, 20mg/kg of TQ, 2.5mg/ kg of cisplatin, a combination of 2.5mg/kg cisplatin and 5mg/kg TQ, 2.5mg/ kg cisplatin and 20mg/kg TQ, and a control group that received no injections of cisplatin and TQ. The mice were injected three times a week and the study was carried out for three weeks. TQ alone at 5mg4g and 20mg/kg did not significantly reduce tumor size. However, cisplatin at 2.5mg/kg reduced tumor size significantly as did the combinations of cisplatin and TQ. The combination of 2.5mg/kg cisplatin and 5mg/kg TQ reduced tumor size by 59% and the 2.5mg/kg cisplatin and 20mg/kg TQ caused a 79% reduction in tumor size. CENTENARY COLLEGE I9TH ANNUAL STUDENT RESEARCH FORUM 7 Posters Does phosphorylation of an activation loop SERINE INHIBIT CKI ACTIVITY? *TAYL0R PAHLS, AHMAD AZZAWE, CHRIS BRYAN, BETHANY JOSEPH, MK ORSULAK, AND JOSHUA PHILLIPS Researcher Advisors: Dr. Cynthia J. Brame^ & Dr. Lucy C. Robinson^ ^Department of Biology Centenary College of Louisiana ^Louisiana State University Health Sciences Center Shreveport, LA The CKI protein kinase family is vital to biological function because these protein kinases regulate chromosome segregation, circadian rhythms, cell division, and cell differentiation. These proteins have a long activation loop, and phosphorylation of this loop may regulate protein activity. We hypothesi ed that 2 the amino acid serine located within the activation loop (S243) is an inhibitory phosphorylation site. Several mutations were created at this spot via site-directed mutagenesis using YCK2 as a model of the CKI protein family. The serine was mutated to alanine, aspartate, and tyrosine. Our hypothesis predicts that the alanine substitution will increase YCK2 activity because alanine has no hydroxyl group and therefore cannot be phosphorylated. We further predict that aspartate substitution will increase YCK2 activity because the negatively charged carboxyl group found on aspartate allows it to mimic phosphorylated serine. Finally, we predict that the tyrosine substitution will have no effect on Yck2 activity because tyrosine has a hydroxyl group that can be phosphorylated. We cloned the three mutant alleles and expressed them in the ye.'asiSaccharomyces ceremiae to examine YCK2 function and localization. The Effect of Antidepressants on Food Intake and Weight of Mice (Mus musculus) JOSHUA PHILLIPS Research Advisor: Dr. Beth Leuck Department of Biology Centenary College of Louisiana Antidepressants like Zoloft and Buspar may be excreted in human urine and end up in the water system because they are not treated using current sewage methods. These drugs are selective serotonin reuptake inhibitors which inhibit serotonin transporter proteins in the brain, prolonging the amount of time serotonin remains in a synapse. Since serotonin is an anorexigenic neurotransmitter, it can lead to decreased appetite causing a reduction in food intake and weight. I hypothesized that if a mouse is given an antidepressant, then its food intake and weight will decrease. In addition, a cocktail of antidepressants will cause a greater decrease than an individual antidepressant. Mice were exposed to a low dosage of a placebo, Zoloft, Buspar, and a cocktail of Zoloft and Buspar. The food intake, weight, and oxygen consumption of the mice were measured. The mice’s food intake was unaffected, but the weight and oxygen consumption of the mice given antidepressants increased. Since the food intake was approximately the same for all groups, the mice given antidepressants may have used the extra energy from their high oxygen consumption for anabolism to put on more weight. These results warrant further testing of pharmaceuticals in the environment. CENTENARY COLLEGE I9TH ANNUAL STUDENT RESEARCH EORUM