BRAIN RESEARCH PROTOCOLS R Brain Research Protocols Vol. 4 (1999) 441-443 CONTENTS OF BRAIN RESEARCH PROTOCOLS VOLUME 4 VOL. 4 NO. 1 APRIL 1999 Radiological determination of the posterior limits of the temporal lobe for volumetric analysis C.P. Doherty, G.E. Meredith, M. Farrell, J. Toland and H. Staunton (Ireland) Quantitative analysis of the effects of lithium on the reverse tolerance and the c-Fos expression induced by methamphetamine in mice M. Namima, K. Sugihara, Y. Watanabe, H. Sasa, T. Umekage and K. Okamoto (Japan) Extraction and quantification of thyroid hormones in selected regions and subcellular fractions of the rat brain G. Pinna, L. Hiedra, H. Prengel, O. Broedel, M. Eravci, H. Meinhold and A. Baumgartner (Germany) Non-isotopic in situ hybridization of CD44 transcript in formalin-fixed paraffin-embedded sections S.O. Suzuki and T. Iwaki Japan) Combination of immunohistochemical and in situ hybridization methods to reveal tyrosine hydroxylase and oxytocin and vasopressin mRNAs in magnocellular neurons of obese Zucker rats S.O. Fetissov and F. Marsais (USA, France) A method to monitor motor cortical excitability in human stroke through motor evoked potentials R. Traversa, P. Cicinelli, M. Filippi, M. Oliveri, M.G. Palmieri, P. Pasqualetti and P.M. Rossini (Italy) Temporally structured impulse activity in spontaneously discharging somatosensory cortical neurons in the awake cat: recognition and quantitative description of four different patterns of bursts, post-recording GFAP immunohistology and computer reconstruction of the studied cortical surface A.A. Miasnikov, H.H. Webster and R.W. Dykes (Canada) Accurate quantification of the mRNA of NMDARI splice variants measured by competitive RT-PCR A. Winkler, B. Mahal, W. Zieglginsberger and R. Spanagel (Germany) Rational design of oligonucleotide probes to avoid optimization steps in in situ hybridization M. Erdtmann-Vourliotis, P. Mayer, U. Riechert, M. Hiindel, J. Kriebitzsch and V. Héllt (Germany) Quantitation of nicotinic acetylcholine receptor subunits «4 and 82 messenger RNA in postmortem human brain using a non-radioactive RT-PCR and CCD imagirg system K. Utsugisawa, H. Tohgi, M. Yoshimura, Y. Nagane and M. Ukitsu (Japan) Metabolism in single rat brain slices measured by magnetic resonance spectroscopy C. Gasparovic, D. King and D.M. Feeney (USA) 97 Corrigendum to ‘A sensitive bioassay for enzymes that synthesize retinoic acid’ [Brain Res. Protocols 1 (1997) 232-236] P. McCaffery and U.C. Drager (USA) 103 VOL. 4 NO. 2 JULY 1999 Protocols Dual intracellular recording of neocortical neurons in a neuron—glia co-culture system K. Nakanishi, M. Nakanishi and F. Kukita (Japan) Whole-mount procedures for simultaneous visualization of nerves, neurons, cartilage and bone E. Rosa-Molinar, B.J. Proskocil, M. Ettel and B. Fritzsch (USA) Extracellular perfusion of rat brain nuclei using microdialysis: a method for studying differential neurotransmitter release in response to neurotoxins N.C. Reynolds Jr., W. Lin, C.M. Cameron and D.L. Roerig (USA) Semi-quantitative reverse-transcriptase polymerase chain reaction: an approach for the measurement of target gene expression in human brain L. Chen, D.M. Segal and D.C. Mash (USA) Neuronal apoptosis studied by a sequential TUNEL technique: a method for tract-tracing A. Hara, M. Niwa, T. Iwai, M. Nakashima, Y. Bunai, T. Uematsu, N. Yoshimi and H. Mori (Japan) A simple method to obtain retinal cell preparations highly enriched in specific cell types. Suitability for lipid metabolism studies M.E. Guido, D.F. Bussolino, G.A. De Arriba Zerpa, S. Deza, S.J. Pasquaré, N.M. Giusto and B.L. Caputto (Argentina) An improved method for dissociation and aggregate culture of human fetal brain cells in serum-free medium A. Barnea and J. Roberts (USA) Recording of long-term potentiation in single dorsal horn neurons in vivo in the rat F. Svendsen, L.J. Rygh, J. Gjerstad, A. Fiska, K. Hole and A. Tjolsen (Norway) A simple in vitro model of ischemia based on hippocampal slice cultures and propidium iodide fluorescence J.H. Laake, F.-M. Haug, T. Wieloch and O.P. Ottersen (Norway, Sweden) High resolution quantitation of microvascular plasma perfusion in non-ischemic and ischemic rat brain by laser-scanning confocal microscopy D.C. Morris, Z. Zhang, K. Davies, J. Fenstermacher and M. Chopp (USA) 442 Contents Volume 4 Measurement of cholecystokinin release in vivo in the rat spinal dorsal horn H. Gustafsson, G. De Araujo Lucas, E. Schétt, C.-O. Stiller, P. Alster, Z. Wiesenfeld-Hallin and E. Brodin (Sweden) Advantage of double labeled in situ hybridization for detecting the effects of glucocorticoids on the mRNAs of protooncogenes and neural peptides (TRH) in cultured hypothalamic neurons L.-G. Luo and I.M.D. Jackson (USA) Effects of astrocytes on neuronal attachment and survival shown in a serum-free co-culture system X.F. Wang and M.S. Cynader (Canada) Continuous infusion of proinflammatory cytokines into the brain to study brain cytokine induced local and systemic immune effects B. Schéning, P. Elepfandt, W.R. Lanksch, H.-D. Volk and C. Woiciechowsky (Germany) Application and modulation of a permanent hydrogen peroxide-induced oxidative stress to cultured astroglial cells J. Hirrlinger, B. Hamprecht and R. Dringen (Germany) (H]-Girisopam, a novel selective benzodiazepine for the 2,3-benzodiazepine binding site E.J. Horvath, C. Salamon, A. Bakonyi, M.LK. Fekete and M. Palkovits (Hungary) VOL. 4 NO. 3 DECEMBER 1999 Interactive Report Organotypic slice cultures for analysis of proliferation, cell death, and migration in the embryonic neocortex T.F. Haydar, L.L. Bambrick, B.K. Krueger and P. Rakic (USA) Protocols Organotypic culture system of chicken retina A. Hoff, H. Himmerle and B. Schlosshauer (Germany) A model of cochlear function assessment during reversible ischemia in the Mongolian gerbil T. Mom, P. Avan, P. Bonfils and L. Gilain (France) Detection of ecto-ATPase activity in synaptic plasma membranes for studying extracellular ATP-induced signal transduction V.S. Zinchuk, T. Okada and T. Kobayashi (Japan) Detection of reactive oxygen species in primary cultures of cerebellar granule cells A. Atlante and S. Passarella (Italy) A method for detection of a cytokine and its mRNA in the central nervous system of the developing rat T. Suzuki, A. Ogata, K. Tashiro, K. Nagashima, M. Tamura, K. Yasui and J. Nishihira (Japan) Evaluation of free radical production, mitochondrial membrane potential and cytoplasmic calcium in mammalian neurons by flow cytometry F.X. Sureda, C. Gabriel, J. Comas, M. Pallas, E. Escubedo, J. Camarasa and A. Camins (Spain) A novel method to determine the localization of high and low-affinity GABA transporters to the luminal and antiluminal membranes of brain capillary endothelial cells Y. Zhang and G.-Q. Liu (China) Recovery of high-integrity mRNA from brains of rats killed by high-energy focused microwave irradiation M. Mayne, P.N. Shepel and J.D. Geiger (Canada) Long-term neural recording characteristics of wire microelectrode arrays implanted in cerebral cortex J.C. Williams, R.L. Rennaker and D.R. Kipke (USA) Rapid assay for one-run determination of purine and pyrimidine nucleotide contents in neocortical slices and cell cultures M. Pissarek, R. Reinhardt, C. Reichelt, A. Manaenko, G.-J. Krauss and P. Illes (Germany) Application of the polymerase chain reaction to the RNase protection assay for 5-HT,, receptor mRNA levels measurement in rat brain tissues C. Drogou, M. Chennaoui, L. Forestier, B. Grimaldi, G. Fillion and Y. Guezennec (France) Quantitative investigation of mitochondrial function in single rat hippocampal slices: a novel application of high-resolution respirometry and laser-excited fluorescence spectroscopy A. Kudin, S. Vielhaber, H. Beck, C.E. Elger and W.S. Kunz (Germany) A rapid and efficient method for preparation of genomic DNA suitable for analysis of both high and low molecular weight DNA fragmentation during neuronal apoptosis V. Solovyan and A. Salminen (Finland, Ukraine) Quantitation of GABA transporter 3 (GAT3) mRNA in rat brain by competitive RT-PCR M. Fickova, N. Dahmen, C. Fehr and C. Hiemke (Germany) Anatomical and functional imaging of the auditory cortex in awake mustached bats using magnetic resonance technology K. Kamada, J.J. Pekar and J.S. Kanwal (USA) A global hypoxia preconditioning model: neuroprotection against seizure-induced specific gravity changes (edema) and brain damage in rats M.R. Emerson, S.R. Nelson, F.E. Samson and T.L. Pazdernik (USA) Detection of MRNA species in bulbospinal neurons isolated from the rostral ventrolateral medulla using single-cell RT-PCR A.M. Comer, H.M. Gibbons, J. Qi, Y. Kawai, J. Win and J. Lipski (New Zealand) Neurochemical determination of the location of NMDA and GABA receptors on rat striatal cholinergic neurons Y. Tkarashi, M. Yuzurihara, A. Takahashi, H. Ishimaru and Y. Maruyama (Japan) The in vivo minigene approach to analyze tissue-specific splicing O. Stoss, P. Stoilov, A.M. Hartmann, O. Nayler and S. Stamm (Germany) Contents Volume 4 Multiplex semi-quantitative reverse transcriptase—polymerase chain reaction of low abundance neuronal mRNAs R. Pernas-Alonso, F. Morelli, U. di Porzio and C. Perrone-Capano (Italy) Quantification of neurotrophin-3 mRNA in the rat hippocampal subregions using the RT-PCR-based coamplification method W. Tokuyama, T. Hashimoto, Y.X. Li, H. Okuno and Y. Miyashita (Japan) Measurement of total nitric oxide metabolite (NO, ) levels in vivo B. Lei, N. Adachi, T. Nagaro and T. Arai (Japan) A method for the determination of activated receptor phosphorylation state following in vivo drug treatment M.A. Bernstein and S.P. Welch (USA) Author Index Contents of Brain Research Protocols Volume 4