Zootaxa 4303 (4): 451–481 ISSN 1175-5326 (print edition) Article ZOOTAXA http://www.mapress.com/j/zt/ Copyright © 2017 Magnolia Press ISSN 1175-5334 (online edition) https://doi.org/10.11646/zootaxa.4303.4.1 http://zoobank.org/urn:lsid:zoobank.org:pub:742CEFD6-6343-41A0-AD5D-F72F1AFE135B Black fungus gnats (Diptera: Sciaridae) of Queensland, Australia. Part I. Genera Chaetosciara Frey, Corynoptera Winnertz, Cratyna Winnertz, Epidapus Haliday, Keilbachia Mohrig, Lobosciara Steffan, Phytosciara Frey and Scatopsciara Edwards WERNER MOHRIG1, ELLEN KAUSCHKE2 & ADAM BROADLEY3,4 1Werner Mohrig, Puddemin 6, 18574 Poseritz/Ruegen, Germany. E-mail: [email protected] 2Ellen Kauschke, Justus-Liebig-University Giessen, Institute of Zoology, Stephanstrasse 24, 35390 Giessen, Germany. E-mail: [email protected] 3Adam Broadley, Department of Agriculture and Water Resources, PO Box 1006, Tullamarine, VIC 3043, Australia. E-mail: [email protected] 4Corresponding author Abstract This study is the first of a taxonomic series on the Sciaridae of Australia, starting with the sciarid fauna of Queensland. Twelve species described herein are new to science. These are Chaetosciara recondita Mohrig & Kauschke sp. n., Craty- na adulterina Mohrig & Kauschke sp. n., Cr. flagriola Mohrig & Kauschke sp. n., Cr. flavothoracica Mohrig & Kauschke sp. n., Cr. livida Mohrig & Kauschke sp. n., Cr. longipeda Mohrig & Kauschke sp. n., Cr. pullata Mohrig & Kauschke sp. n., Epidapus excelsus Mohrig & Kauschke sp. n., Keilbachia adstrictatula Mohrig & Kauschke sp. n., Scatopsciara brevicolla Mohrig & Kauschke sp. n., Sc. dubiosa Mohrig & Kauschke sp. n., and Sc. validovenosa Mohrig & Kauschke sp. n. Seven species are new reports for Australia. These are Cratyna flagria Mohrig, Cr. vera Mohrig, Phytosciara bella Mohrig, Ph. conturbata Mohrig, Ph. pseudoornata Mohrig and Corynoptera gladiota Mohrig, previously described from Papua New Guinea, and Lobosciara trilobata Vilkamaa & Hippa, described from Sulawesi. Key words: Chaetosciara, Corynoptera, Cratyna, Epidapus, Keilbachia, Lobosciara, Phytosciara, Scatopsciara, Diver- sicratyna, Pictosciara, new species, Australia, Queensland Introduction The Sciaridae of Australia have attracted relatively less attention from entomologists compared to other groups of nematoceran flies, except some sciarid pest species that infest cultivated mushrooms and plants (Greenslade & Clift 2004). The first taxonomic work on Australian Sciaridae was published by Skuse nearly 130 years ago, when he described 59 species: 57 as Sciara, one as Trichosia and one as Zygoneura (Skuse 1888; 1890). Schmitz & Mjöberg (1924) described Austrosciara termitophila from a dampwood termite nest and Loudon (1978) described a Lycoriella pest of mushrooms as new (L. agarici); this was subsequently determined to be a synonym of L. sativae (Johannsen). The Catalog of the Diptera of the Australasian and Oceanian Regions lists 63 species (mostly as Sciara) for Australia/Tasmania, without any generic revision (Steffan 1989). The Australian sciarid fauna is obviously much richer than presented in the regional literature. Our revision of 27 male types from the Skuse collection revealed that 14 species belonged to the genus Bradysia Winnertz, one species to the genus Corynoptera Winnertz, four species to the genus Austrosciara Schmitz & Mjöberg, two species to the genus Pseudolycoriella Menzel & Mohrig, one species to the genus Pseudozygomma Mohrig, one species to the genus Sciara Meigen and one species to the genus Scythropochroa Enderlein (Broadley et al. 2016). Herein we present a regional faunistic study on sciarids of the wet tropics of North-East Queensland and this is one of the first steps in a revisionary work on the Australian Sciaridae. The rain forest of North Queensland is one of the oldest wet forests in the world and is closely connected to Accepted by K. Heller: 12 Jun. 2017; published: 11 Aug. 2017 451 Licensed under a Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0 comparative biotopes in Papua New Guinea. The sciarid fauna of Papua New Guinea was studied recently by Mohrig (1999, 2004, 2013, 2016), that of New Caledonia by Vilkamaa et al. (2011, 2012a, 2012b, 2012c, 2012d, 2014, 2015) as well as by Köhler & Menzel (2013) and that of New Zealand by Mohrig & Jaschhof (1999) and Köhler and Mohrig (2016). These reports can be used as a preliminary framework with which to undertake a comparative analysis concerning respective environments on the Australian mainland. New Guinea acts as a faunistic bridge between the northern Australasian region and Asia on one side (Brunetti 1912; Edwards 1928, 1929, 1931; Menzel & Smith 2009) and to New Caledonia and Oceania on the other (Steffan 1969). Materials and methods Localities. The following taxonomic study is based on sciarid material collected from North Queensland. Most were selected from Malaise traps that James Seymour (James Cook University, Cairns) used to sample two different mesophyll vine forest areas near Port Douglas and Kuranda in 1997. The remainder were caught in 2000 by sweep net near Cairns and in the Tablelands by Werner Mohrig. The first sampling area for Malaise traps was Mt Lewis, located 37 km WSW of Port Douglas (16°35´S, 145°16´E) within state forest boundaries adjacent to the Daintree River National Park, with an altitude of about 1200 m. The second area was the Black Mountain Road, off the Kennedy Highway before Kuranda, 28 km from Cairns. Preparation. The specimens were stored in 70% ethanol and embedded in Canada balsam after dehydration in 96% ethanol (at least 10 min), followed by treatment in beechwood creosote (30 min). From creosote they were then transferred with needles to the middle of a microscope slide. The hypopygium was dissected and transferred separately with the help of a needle into a small drop of Canada balsam, placed next to the body on the slide. Under the control of a stereomicroscope the hypopygium was arranged with the ventral side upwards and covered with a 5 x 5 mm cover slip. After that a larger drop of Canada balsam was put on the body and covered with a 10 x 10 mm cover slip. In some cases one wing was dissected and mounted separately, too. Figures. Illustrations were obtained on the basis of multilayer digital images using a Keyence VHX-2000 digital microscope and respective software, including size measurements. Photographs obtained were modified using Adobe Photoshop software. Print details were improved by hand drawing and simultaneous microscopic control (Olympus microscope). After final scanning and corrections using Photoshop software they were finalized for publication. The scale bars in the figures only refer to the male genitalia and the habitus pictures. Determination. The terminology used herein follows Menzel & Mohrig (1997) and Mohrig et al. (2013). Morphological details are illustrated in Broadley et al. (2016) and Menzel & Mohrig (2000). A key to genera of Sciaridae in the Australasian region does not currently exist but keys to genera of the Palaearctic region may be useful [Menzel & Mohrig (1997), in English; Menzel & Mohrig (2000), in German] since most of the Australian species belong to genera that are found in the northern hemisphere. Holotypes are stored in the Private Collection of Werner Mohrig, Puddemin, Germany (PWMP), some paratypes and specimens for comparison are in the Australian National Insect Collection, Canberra (ANIC) as well as in the Private Collection of Adam Broadley, Melbourne (PABM). Museums ANIC Australian National Insect Collection, CSIRO, Canberra, Australia PABM Private Collection of Adam Broadley, Melbourne, Australia PKHH Private Collection of Kai Heller, Heikendorf, Germany PWMP Private Collection of Werner Mohrig, Puddemin, Germany SDEI Senckenberg German Entomological Institute Müncheberg, (Senckenberg Deutsches Entomologisches Institut), Müncheberg, Germany Abbreviations: l/w-index = length/width of the basal node of 4th flagellomere; c/w = ratio of C and w within the space between R and M; x/y = wing vein bM/wing vein r-m. 5 1 452 · Zootaxa 4303 (4) © 2017 Magnolia Press MOHRIG ET AL. Results Genus Chaetosciara FREY, 1942 Type species: Sciara fenestralis Lengersdorf sensu Frey, 1942 [Notul. Ent. 22: 33] = [Sciara estlandica Lengersdorf, 1929]. Literature: Mohrig (1999): 198–200; Menzel & Mohrig (2000): 508–515; Rudzinski (2006): 463–467. Chaetosciara recondita Mohrig & Kauschke sp. n. (Fig. 1 A–D) Type locality: Australia, Queensland, Mt Lewis, wet forest, 37 km WSW of Port Douglas, 16°35´S, 145°16´E. Holotype: Male, 13.vi.1997, Malaise trap, leg. J. Seymour (PWMP). Description. Male. Head. Brown. Eye bridge 4 facets wide. Flagellomeres brown with a net-like surface, dense pale hairs shorter than the width of the basal node, necks whitish; 4th flagellomere with l/w index of 2.0. Palpus rather long, three-segmented; basal segment large, with 6–7 bristles and a patch of long sensillae. Thorax. Brown. Scutum with rather short and fine hairs, some lateral hairs longer; scutellum with short hairs and 4 longer marginal bristles. Postpronotum bare. Wing pale, R = 2/3 R, joining C before the M-fork; R in the distal third with 1 5 ventral macrotrichia; y longer than x and with 1–2 macrotrichia; CuA-stem short; posterior veins without macrotrichia. Haltere short, brownish. Legs yellowish; fore tibiae with a few longer bristles within the ground hair, at the apex with a large and dense patch of pale bristles; spurs of the middle and hind tibiae equal, yellowish and longer than the diameter of the apex. Claws toothless. Abdomen. Hypopygium brownish, the intergonocoxal space with a weak membranous lobe that is short and pyramid-like in shape; gonocoxites on ventral apex with a long, strong bristle, the inner ventral margin with rather long hairs; gonostylus shorter than gonocoxites, externally rounded, internally somewhat flattened; apically with 4 (sometimes 5) short dark spines, subapically with a short whiplash-like hair. Tegmen rounded, with an area of fine teeth and a thin finger-like protuberance subapically. Aedeagus rather long and robust. Body length: 3 mm. Comments. The species is characterized by short and robust flagellomeres with a distinct net-like surface, pale hairs and whitish necks. The hypopygium has a weak membranous intergonocoxal lobe and the gonostylus is ovoid in shape with 4–5 short dark spines at the apex. The tegmen is apically rounded with a thin finger-like structure. A similar species is not known yet. Distribution. Australia, Queensland. Genus Corynoptera WINNERTZ, 1867 Type species: Corynoptera perpusilla Winnertz, 1867: 177; [preocc., nec Corynoptera perpusilla (Walker, 1848); = Corynoptera fatigans (Johannsen, 1912)]. Literature: Tuomikoski (1960): 42–73; Mohrig & Jaschhof (1999): 44–87; Menzel & Mohrig (2000): 205–260; Hippa et al. (2010): 1–197. Corynoptera gladiota Mohrig, 2004 (Fig. 2 A–C) Literature: Mohrig (2004): 162, fig. 29 a–c. Material: 2 males, 2.vii.2000, Australia, Queensland, surroundings of Cairns, Mount Malloy near Kuranda, wet tropics, leg. W. Mohrig (PWMP). Comments. The species is characterized by flagellomeres with a rough surface and very long bristles, much longer than the diameter of the basal node, a nearly comb-like row of bristles on the apex of the fore tibia, and a gonostylus with 3 short robust apical spines as well as a long spine on a large lobe in the basal half, near the base of the gonostylus. Specimens from Queensland are identical with C. gladiota Mohrig from Papua New Guinea SCIARIDAE OF QUEENSLAND I Zootaxa 4303 (4) © 2017 Magnolia Press · 453 FIGURE 1. Chaetosciara recondita Mohrig & Kauschke sp. n. A. Hypopygium; B. Ventral base of hypopygium; C. Flagellomeres 4–5; D. Apex of fore tibia. 454 · Zootaxa 4303 (4) © 2017 Magnolia Press MOHRIG ET AL. FIGURE 2. Corynoptera gladiota Mohrig, 2004. A. Gonostylus; B. Flagellomeres 4–5; C. Apex of fore tibia. in all morphological details. The species belongs to the C. parvula group, which is species-rich and mainly distributed in the Holarctic region. Distribution. Australia, Queensland; Papua New Guinea. Genus Cratyna Winnertz, 1867 Type species: Cratyna atra Winnertz, 1867: 167, fig. 7. SCIARIDAE OF QUEENSLAND I Zootaxa 4303 (4) © 2017 Magnolia Press · 455 Literature: Tuomikoski (1960): 31–41 (as Plastosciara); Hippa et al. (1998): 1–86 (as Pseudozygoneura); Menzel & Mohrig (1998): 362 (as Cratyna Winnertz); Mohrig (1999): 167–182; Menzel & Mohrig (2000): 185–191; Mohrig (2004): 141– 144; Vilkamaa & Hippa (2005): 457–480; Mohrig & Menzel (2014): 138–143; Shin et al. (2014): 344–354; Huang et al. (2015): 77–95 (as Pseudozygoneura). The genus currently contains the following subgenera: Cratyna Winnertz, 1867 s. str. (distributed worldwide), Diversicratyna Menzel & Mohrig, 1998 (mainly Holarctic), Peyerimhoffia Kieffer, 1903 (Holarctic), Pictosciara Mohrig, 2004 (Southern hemisphere) and Spathobdella Frey, 1948 (Holarctic). Cratyna (Diversicratyna) adulterina Mohrig & Kauschke sp. n. (Fig. 3 A–B) FIGURE 3. Cratyna (Diversicratyna) adulterina Mohrig & Kauschke sp. n. A. Hypopygium; B. Apex of fore tibia. 456 · Zootaxa 4303 (4) © 2017 Magnolia Press MOHRIG ET AL. Type locality: Australia, Queensland, Mt Lewis, 37 km WSW of Port Douglas, 16°35´S, 145°16´E, wet tropics. Holotype: Male, 8.viii.1997, Malaise trap, leg. J. Seymour (PWMP). Description. Male. Head. Yellowish-brown. Eye bridge 4 facets wide. Antennal flagellomeres with short dense hair, rather short necks; 4th flagellomere with l/w index of 2.2, neck ¼ of the length of the basal node, brownish and darkened at the tip; with hairs shorter than the diameter of the basal node. Palpus short, 2-segmented; basal segment with 1–2 bristles. Thorax. Brown with yellow spots on the scutum and pleural sclerites. Scutum with rather short hairs and some longer lateral bristles; scutellum with short hairs and with 4 longer marginal bristles. Postpronotum with a few short hairs. Wing brownish, R = R, joining C at the level of the M-fork; R with 1 5 ventral macrotrichia in the distal third; y = x, without macrotrichia; posterior veins without macrotrichia. Haltere rather long, brown. Legs yellowish, tarsi darkened; legs rather long and thin; fore tibia with a dense patch of brownish bristles at the inner apex; spurs of middle and hind tibiae equal and much longer than the diameter of the apex; claws without teeth. Abdomen. Tergites brown, sclerites yellow. Hypopygium brownish; gonocoxites somewhat longer than gonostylus, without a distinct long robust bristle on the ventral apex, the inner ventral margin with rather sparse hairs; gonostylus narrowed to the apex, with a long apical tooth and 4 shorter hyaline spines (two above and two below the tooth). Tegmen laterally curved, without any inner structure. Aedeagus short. Body length: 3.4 mm. Comments. Cratyna adulterina sp. n. is similar to Cratyna unispinula (Mohrig & Menzel, 1992) from Germany. It is characterized by a 2-segmented palpus, a rather long-stalked haltere and a narrow gonostylus with a long apical tooth and four hyaline spines. The subgenus Diversicratyna was established for Palaearctic species and differs mainly from other Cratyna subgenera by a narrow elongate gonostylus with a rather strong apical tooth, surrounded by a few shorter spines. The zoogeographic distribution of the subgenus is not clear yet and requires further investigation, particularly in relation to the fauna of the Oriental region. Distribution. Australia, Queensland. Cratyna (Cratyna) flagria Mohrig, 1999 (Fig. 4 A–D) Literature: Mohrig (1999): 179–180, fig. 23 a–c. Material: 1 male, 13.vi.1997, Queensland, Mt Lewis, 37 km WSW of Port Douglas, 16°35´S, 145°16´E, wet tropics; 1 male, 8.viii.1997, same locality, Malaise trap, leg. J. Seymour (PWMP; PABM). Comments. The species is characterized by flagellomeres with short basal nodes and two irregular rows of very long, cecidomyiid-like bristles and necks nearly as long as the basal node. Palpus 2-segmented, short and small. Gonocoxite short, intergonocoxal space closed, gonostylus larger than gonocoxite, elongate-oval and compact, with 5 short hyaline apical/subapical spines. Tegmen with a funnel-like apical structure. Distribution. Australia, Queensland; Papua New Guinea. Cratyna (Cratyna) flagriola Mohrig & Kauschke sp. n. (Fig. 5A–C) Type locality: Queensland, near Cairns, Palm Cove, mangrove forest at the coastline. Holotype: Male, 3.vii.2000, caught by net, leg. W. Mohrig (PWMP). Description. Male. Head. Yellowish-brown. Eye bridge 3–4 facets wide. Antennal flagellomeres with long necks and very long cecidomyiid-like bristles, arranged in three nearly circular whorls; 4th flagellomere with l/w index of 1.5, bristles about 3 times longer than the diameter of the basal node, neck as long as the basal node, pale but darkened at the tip; palpus short, three-segmented; basal segment larger, with 3 bristles and a patch of long sensillae. Thorax. Ochrous, with darker spots on lateral sclerites. Scutum with rather fine hairs and some longer lateral bristles; scutellum with 4 longer marginal bristles. Postpronotum bare. Wing pale, R =3/4 R, joining C 1 before the M-fork; R with ventral macrotrichia in the apical third; y longer than x and with 2–3 macrotrichia; 5 posterior veins without macrotrichia. Haltere short, brownish. Coxae and femora yellowish, tibiae and tarsi SCIARIDAE OF QUEENSLAND I Zootaxa 4303 (4) © 2017 Magnolia Press · 457 FIGURE 4. Cratyna (Cratyna) flagria Mohrig. A. Hypopygium; B. Male in toto; C. Flagellomeres 3–6; D. Apex of fore tibia. 458 · Zootaxa 4303 (4) © 2017 Magnolia Press MOHRIG ET AL. FIGURE 5. Cratyna (Cratyna) flagriola Mohrig & Kauschke sp. n. A. Hypopygium; B. Pedicel and flagellomeres 1–4; C. Apex of fore tibia. SCIARIDAE OF QUEENSLAND I Zootaxa 4303 (4) © 2017 Magnolia Press · 459 darkened; legs rather long; fore tibia with a large patch of dark bristles at the inner apex; spurs of middle and hind tibiae equally long and much longer than the diameter of the apex; claws toothless. Abdomen. Brown. Hypopygium brown; gonocoxite short and as long as gonostylus, with a long, fine ventral bristle, at the inner ventral margin rather sparsely haired; gonostylus large and ovoid, on the inner side with two pairs of hyaline spines, widely separated from each other. Tegmen somewhat broader than long, rounded, with a weak inner semicircular structure and a short finger-like protuberance. Aedeagus short. Body length: 2.6 mm. Comments. This new species is characterized by long necks and long, circular arranged whorls of bristles on the flagellomeres, a 3-segmented palpus and an ovoid gonostylus with 2 pairs of hyaline spines. The unique cecidomyiid-like flagellomeres are typical for several species found in the Malay Archipelago (Hippa et al. 1998, as Pseudozygoneura), Papua New Guinea (Mohrig 1999) and Central America (Mohrig 2003). The species is similar to Cr. flagriantennata Mohrig from Papua New Guinea (Mohrig 1999). It differs because of a broader space between the two pairs of spines at the apex of the gonostylus, a three-segmented palpus and a yellow body colour. Cratyna (Cratyna) flavothoracica Mohrig & Kauschke sp. n. (Fig. 6 A–B) Type locality: Australia, Queensland, Mt Lewis, 37 km WSW of Port Douglas, 16°35´S, 145°16´E, wet tropics. Holotype: Male, 8.viii.1997, Malaise trap, leg. J. Seymour (PWMP). Paratype: 1 male, same data as holotype (PWMP). FIGURE 6. Cratyna (Cratyna) flavothoracica Mohrig & Kauschke sp. n. A. Hypopygium; B. Flagellomeres 3–5. 460 · Zootaxa 4303 (4) © 2017 Magnolia Press MOHRIG ET AL.