Prasanna B.D.  Sathyanarayana N. Gummadi Praveen V. Vadlani E ditors Biotechnology and Biochemical Engineering Select Proceedings of ICACE 2015 Biotechnology and Biochemical Engineering Prasanna B.D. Sathyanarayana N. Gummadi (cid:129) Praveen V. Vadlani Editors Biotechnology and Biochemical Engineering Select Proceedings of ICACE 2015 123 Editors Prasanna B.D. Praveen V.Vadlani Department ofChemical Engineering Department ofChemical Engineering National Institute ofTechnology Karnataka Kansas State University Mangalore, Karnataka Manhattan, KS India USA Sathyanarayana N.Gummadi BhupatandJyoti Mehta School of Biosciences Indian Institute of Technology Madras Chennai, Tamil Nadu India ISBN978-981-10-1919-7 ISBN978-981-10-1920-3 (eBook) DOI 10.1007/978-981-10-1920-3 LibraryofCongressControlNumber:2016947027 ©SpringerScience+BusinessMediaSingapore2016 Thisworkissubjecttocopyright.AllrightsarereservedbythePublisher,whetherthewholeorpart of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission orinformationstorageandretrieval,electronicadaptation,computersoftware,orbysimilarordissimilar methodologynowknownorhereafterdeveloped. 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Preface The Department of Chemical Engineering, National Institute of Technology Karnataka, Surathkal, India was started in the year 1965 with the assistance of UNESCO, MHRD-Government of India, and the Government of Karnataka and is consistentlyrankedasoneofthetopchemicalengineeringdepartmentsinIndiaby theNationalBoardofAccreditation.Thedepartment hascompleted50yearsofits glorious service to the cause of Chemical Engineering Education, Research and Industrial collaboration, and celebrated Golden Jubilee Year in 2015. In order to commemorate this important milestone, International Conference on Advances in Chemical Engineering (ICACE)—2015 was organized during December 20–22, 2015. Chemical engineering is a branch of science that combines physical sciences (physics and chemistry) and life sciences (molecular and synthetic biology) with applied mathematics and economics to synthesize and transform chemicals, mate- rials, and energy from various feedstocks. Essentially, chemical engineers con- ceptualize novel and innovative processes and implement large-scale industrial operations to produce chemicals and materials suited to every need of the society, including food, chemicals, energy, environment, and information. ICACE-2015 conference provided a platform for deliberating advances in chemical engineering aswellasalliedfields,throughinvitedlecturesdeliveredbyresearchersofnational and international repute, and paper and poster presentation by participants from academia, research organizations, and industries. ICACE-2015 had solicited technical and research submissions related to Industrial Biotechnology and Biochemical Engineering. Bioprocessing of renew- ableresourcestousefulproductsisasustainableapproachandhasthewherewithal tosolvesomeofthechallengingissuesfacinghumanity.Wehadanoverwhelming response to our call for paper submission, and all the submitted papers were screened and selected by the technical committee on the basis of originality, sig- nificance,coherenceofdataandclarity.Altogether53paperswerereceived;outof which 37 papers were selected for oral presentation in the conference. The papers v vi Preface presentedduringtheconferencewereagainsubjectedtopeer-reviewandplagiarism check. Based on the outcome, 26 papers have been selected for publication in this book. We would like to thank Dr. Keyur Raval for helping us in the peer-review process. Our heartfelt thanks to Dr. Kalaivani, Mrs. Jaya Mary, and Mr. Basavaraj Nainegli for their editorial assistance. We are highly grateful to NITK adminis- tration,TEQIP,andtechnicalcommitteemembersfortheircooperation.Theefforts by faculty, staff, students, and alumni of NITK Surathkal in organizing ICACE-2015 are greatly appreciated. We are grateful to all those who have con- tributed directly and indirectly for the success of this event. We hope that all the readers will find this book volume useful and productive. Karnataka, India Prasanna B.D. Chennai, India Sathyanarayana N. Gummadi Manhattan, USA Praveen V. Vadlani Contents Selection of Medium and Optimization of Process Parameters for Melanin Biosynthesis from Pseudomonas stutzeri HMGM-7 ... .... 1 Harsha Thaira, Shraddha S. Bhosle, Rajmohan Balakrishnan and Keyur Raval Unstructured Kinetic Modeling of Glutathione Production by Saccharomyces cerevisiae NCIM 3345 .... .... .... .... ..... .... 11 Abhinandan Dhavale, Atul Vhanmarathi, Shrinivas Deshmukh and Seema Dabeer Statistical Optimization of Lactic Acid Extraction from Fermentation Broth Using Emulsion Liquid Membrane..... .... 21 Avinash Thakur, Parmjit Singh Panesar and Manohar Singh Saini Optimization of Microwave Assisted Extraction of Pectin from Helianthus annuus Head Using Response Surface Methodology ... .... ..... .... .... .... .... .... ..... .... 35 B.K. Aarthi, V. Aswini, M. Lakshmi Priya, M. Nirosha and M. Shanmugaprakash Production and Characterization of Hydrophobins from Fungal Source .... .... ..... .... .... .... .... .... ..... .... 47 Basavaraj Hungund, Chaitanya Habib, Vaibhav Hiregoudar, Sona Umloti, Saiprasad Wandkar and Gururaj Tennalli Sustainable Utilization of Food Industry Waste and by-Products for the Production of Prebiotic Isomaltooligosaccharides (IMO).... .... .... .... .... .... ..... .... 55 Anindya Basu and S.G. Prapulla Enhancing the Bioproduction of Cellulase by Aspergillus nidulan via Medium Optimization .... .... .... ..... .... 65 P. Saravanan and R. Muthuvelayudham vii viii Contents Modeling and Theoretical Analysis of Isomaltooligosaccharide (IMO) Production Using Fed-Batch Process .... ..... .... .... .... .... .... ..... .... 73 Sarma Mutturi and Anindya Basu Optimization of Na CO Pre-treatment by RSM Approach 2 3 for Releasing Reducing Sugars from Cocoa Pod Shells. .... ..... .... 85 Vinayaka B. Shet, Nisha, Manasa Bhat, Manasa, Leah Natasha S. Mascarenhas, Louella C. Goveas, C. Vaman Rao and P. Ujwal Partitioning of Nitralase Enzyme from Pseudomonas putida in Polymer/Salt Aqueous Two Phase System . .... .... .... ..... .... 93 V. Lokesh Ramana, Iyyaswami Regupathi, B.S. Rashmi and S. Nainegali Basavaraj AqueousTwo-Phase(Acetonitrile–PotassiumCitrate) PartitioningofBovineSerumAlbumin:Equilibrium andApplicationStudies............................................... 101 Badekar Sagar Dilip, Regupathi Iyyaswami, Basavaraj S. Nainegali and B.S. Rashmi Mixed Surfactant Based Reverse Micelle Extraction of Lactose Peroxidase from Whey.. .... .... .... .... .... ..... .... 111 Sivananth Murugesan, Prudhvi Ambakam, Akshay Naveen, Aarathi Makkada, Nithin Solanki and Regupathi Iyyaswami Evaluation of Bio-surfactant on Microbial EOR Using Sand Packed Column.. ..... .... .... .... .... .... ..... .... 121 A. Rajesh Kanna, Sathyanarayana N. Gummadi and G. Suresh Kumar Extraction of Polyphenols from Orange Peel by Solvent Extraction and Microbial Assisted Extraction and Comparison of Extraction Efficiency.. .... ..... .... .... .... .... .... ..... .... 129 Prabha Hegde, Pushpa Agrawal and Praveen Kumar Gupta Enzymatic Concentration of n−3 Polyunsaturated Fatty Acids from Indian Sardine Oil.... .... .... .... .... ..... .... 137 Charanyaa Sampath, N. Anita, B.D. Prasanna and Iyyaswami Regupathi High-Throughput Screening of Cell Repellent Substrate Chemistry for Application in Expanded Bed Adsorption Chromatography... .... .... ..... .... .... .... .... .... ..... .... 145 Vikas Yelemane, Martin Kangwa and Marcelo Fernández-Lahore Concentration of C-Phycocyanin from Spirulina platensis Using Forward Osmosis Membrane Process.. .... .... .... ..... .... 153 Shoaib A. Sharief and Chetan A. Nayak Contents ix Design and Fabrication of Miniature Bubble Column Bioreactor for Plant Cell Culture .. .... .... .... .... .... ..... .... 163 K. Sandesh, P. Ujwal, Blecita D. Mascarenhas, Gayatri Dhamannavar, Narmada Kumar and Dakshayini Industrial Applications of Caffeine Degradation by Pseudomonas sp. .... .... ..... .... .... .... .... .... ..... .... 171 Swati Sucharita Dash, Sree Ahila Retnadhas, Nameeta Rao and Sathyanarayana N. Gummadi Highly Sensitive Determination of Ascorbic Acid, Dopamine and Uric Acid Using Mesoporous Nitrogen Containing Carbon . ..... .... .... .... .... .... ..... .... 179 Anju Joshi and C.N. Tharamani Nano-aptamer Based Quantitative Detection of Chloramphenicol. .... .... ..... .... .... .... .... .... ..... .... 187 Richa Sharma, K.V. Ragavan, K.S.M.S. Raghavarao and M.S. Thakur Influence of Substrate Concentration, Nutrients and Temperature on the Biodegradation of Toluene in a Differential Biofilter Reactor... .... .... .... .... .... ..... .... 197 Suganya Baskaran, Shri Vaishnavi Perumal Selvakumar, Roshni Mohan, Rhea Mariam John, Swaminathan Detchanamurthy, Meyyappan Narayanan and Peter Alan Gostomski Importance of Biomass-Specific Pretreatment Methods for Effective and Sustainable Utilization of Renewable Resources.. .... 207 Yadhu N. Guragain and Praveen V. Vadlani Medium Optimisation for Maximum Growth/Biomass Production of Arthrobacter sulfureus for Biodesulphurisation..... .... 217 E. Asha Rani, M.B. Saidutta and B.D. Prasanna Effect of Electrodeposited Copper Thin Film on the Morphology and Cell Death of E. Coli; an Electron Microscopic Study .. ..... .... 227 Arun Augustin, Harsha Thaira, K. Udaya Bhat and K. Rajendra Udupa Optimization of a Glucocorticoid Encapsulated PLGA Nanoparticles for Inflammatory Diseases .... .... .... .... ..... .... 233 Sriprasad Acharya and Bharath Raja Guru About the Editors Dr. Prasanna B.D. is Assistant Professor in the Department of Chemical Engineering, National Institute of Technology Karnataka. He has produced more than 25 research publications in the field of biotechnology and two patent appli- cations. His research areas include fermentation process development: enzymes, probiotics and nematodes; downstream process technologies; and oenology. Prof. Sathyanarayana N. Gummadi is Professor in the Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of TechnologyMadras.Hisresearchprimarilyfocusesonunderstandingthemolecular and physiological basis of survival of microbes under extreme conditions and exploiting their industrial importance, and on understanding the molecular and biochemical basis of phospholipid translocation in cellular membranes. Dr. Praveen V. Vadlani is the Gary and Betty Lortscher Renewable Energy AssociateProfessorattheDepartmentofGrainScienceandIndustry,KansasState University, USA. He has more than 30 research publications to his credit and is currently working in the advanced chemical engineering field. xi