biophysical journal Published monthly for the Biophysical Society by The Rockefeiier University Press. Edited by the Biophysical Society in cooperation with the Division of Biological Physics of The American Physical Society ee Editorial Board Editor Victor Bloomfield Associate Editors Elias Greenbaum David Levitt David D. Thomas Tian Y. Tsong Associate Editor for Education Norma M. Allewell Richard W. Aldrich Giuseppe iInesi Leonard J. Banaszak Ken Jacobson Joseph M. Beechem John E. Johnson David Beveridge Howard Kutchai Rodney L. Biltonen David Needham Barbara Brodsky Kathryn F. La Noue Robert G. Bryant Ira W. Levin Donald M. Crothers Lee Makowski Louis J. DeFelice John L. Markley Ken A. Dill C. Robert Matthews Thomas G. Ebrey J. Andrew McCammon Donald S. Faber Wilma K. Olson Sidney Fleischer Linda S. Powers Yale E. Goldman M. Thomas Record, Jr. David Gorenstein Gabor Szabo Wayne L. Hubbell Nancy Thompson Sek Wen Hui Douglas H. Turner Volume 63 Rockefeller ° University New York 1992 copyright © 1992 by biophysical society biophysical journal july 1992 volume 63 number 1 Editorial Stuay of mechanisms of electric field-induced DNA transfection ill. Electric parameters and other Editorial. Victor Bloomfield conditions for effective transfection. T. D. Xie and Tian Y. Tsong Channels and Receptors Transport methods for probing the barrier domain Implications of epidermal growth factor (EGF) of lipid bilayer membranes. Tian-xiang Xiang, induced egf receptor aggregation. Carla Wofsy, Xueling Chen, and Bradley D. Anderson Byron Goldstein, Kirk Lund, and H. Steven Wiley Pulmonary surfactant protein SP-C causes Gating of mammalian cardiac gap junction packing rearrangements of channels by transjunctional voltage. dipalmitoylphosphatidyicholine in spread riong-Zhan Wang, Jian Li, Larry F. Lemanski, and monolayers. Jesus Pérez-Gil, Kaushik Nag, Richard D. Veenstra Svetla Taneva, and Kevin M. W. Keough Gramicidin channe! selectivity. Molecular A membrane model for cytosolic calcium mechanics calculations for formamidinium, oscillations. A study using Xenopus oocytes. guanidinium, and acetamidinium. Brian Turano, M. Saleet Jafri, Sandor Vajda, Pedro Pasik, and Michael Pear, and David Busath Boaz Gillo Cadmium block of calcium current in frog Dynamic studies of proton diffusion in mesoscopic sympathetic neurons. Frank Thévenod and heterogeneous matrix. |. Concentrated solutions Stephen W. Jones of sucrose. M. Gutman, E. Nachiiel, and Conditional probability analysis for a domain S. Kiryati model of Ca?*-inactivation of Ca** channels. Dynamic studies of proton diffusion in mesoscopic Joel Keizer and Laura Weiss Maki heterogeneous matrix. Il. The interbilayer space between phospholipid membranes. M. Gutman, E. Nachliel, and S. Kiryati Cell Surface Phenomena Simulation of cell rolling and adhesion on surfaces Electric and Magnetic Field Effects in shear flow: general results and analysis of Dual-frequency dielectrophoretic levitation of selectin-mediated neutrophil adhesion. Canola protoplasts. Karan V. |. S. Kaler, Daniel A. Hammer and Sachin M. Apte Jing-Ping Xie, Thomas B. Jones, and Micromanipulation of adhesion of phorboi 12- Reginald Paul myristate-13-acetate-stimulated T lymphocytes to Brain stimulation using electromagnetic sources: planar membranes containing intercellular theoretical aspects. Leon Heller and adhesion molecule-1. Aydin Tézeren, David B. van Hulsteyn Linda H. Mackie, Michael B. Lawrence, Electrorotation and levitation of cells and colloidal Po-Ying Chan, Michael L. Dustin, and particles. Kenneth R. Foster, Friedrich A. Sauer, Timothy A. Springer and Herman P. Schwan Membranes and Bilayers Photobiophysics Chain-length dependence of lipid bilayer Simulations of the temperature dependence of properties near the liquid crystal to gel phase energy transfer in the PSI core antenna. transition. Michael R. Morrow, Yiwei Jia, John M. Jean, Melanie M. Werst, John P. Whitehead, and Dalian Lu Chi-Kin Chan, and Graham R. Fleming Contents Spectroscopy, Imaging, Other Techniques turbidity observations: clot structure and assembly are kinetically controlled. A semi-empirical approach for the simulation of John W. Weisel and Chandrasekaran Nagaswami circular dichroism spectra of gramicidin A in a model membrane. M. Carmen Bajid, Monomer diffusion and polymer alignment in Lorenzo Braco, and Concepcién Abad domains of sickle hemoglobin. Michael R. Cho and Frank A. Ferrone Resonance Raman spectroscopic and kinetic consequences of a nitrogen - - - sulphur Muscle and Contractility enzyme-substrate contact in a series of dithioacylpapains. P. J. Tonge, B. Gour-Salin, Effect of small release on force during P. Lachance, A. C. Storer, and P. R. Carey sarcomere-isometric tetani in frog muscle Binding kinetics of an anti-dinitrophenyl fibers. A. Horowitz, H. P. M. Wussling, and G. H. Pollack monocional Fab on supported phospholipid monolayers measured by total internal reflection Indo-1 binding to protein in permeabilized with fluorescence photobleaching recovery. ventricular myocytes alters its spectral and Ca Mary Lee Pisarchick, Diane Gesty, and binding properties. Leif Hove-Madsen and Nancy L. Thompson Donald M. Bers A quasi-elastic light scattering study of smooth Proteins muscle myosin in the presence of ATP. Xufeng Wu, Paul S. Blank, and Francis D. Carlson Folding kinetics of designer proteins. Application of the diffusion-collision model to a de novo Excitation-contraction coupling in intact frog designed four-helix bundle. Kanthi K. Yapa and skeletal muscle fibers injected with mmolar David L. Weaver concentrations of fura-2. S. Hollingworth, A. B. Harkins, Nagomi Kurebayashi, M. Konishi, Supramolecular Assemblies and S. M. Baylor Computer modeling of fibrin polymerization kinetics correlated with electron microscope and * Brief communication Biophysical Journal Volume 63 December 1992 biophysical journal august 1992 volume 63 number 2 contents New Techniques Dimerization kinetics of the IgE-class antibodies by divalent haptens. ll. The interactions between Atomic force microscopy of three-dimensional intact IgE and haptens. membrane protein crystals. Ca-ATPase of Reinhard Schweitzer-Stenner, Arieh Licht, and sarcoplasmic reticulum. Jean-Jacques Lacapére, Israel Pecht David L. Stokes, and Didier Chatenay Measuring local surface charge densities in Photobiophysics electrolyte solutions with a scanning force microscope. Hans-Juirgen Butt Virtual intermediates in photosynthetic electron transfer. Julian S. Joseph and William Bialek ..._ 397 Biophysical Theory Oxygen diffusion-concentration product in Long tail kinetics in biophysics?. John F. Nagle rhodopsin as observed by a pulse ESR spin Protein dynamics and 1/f noise. labeling method. Witold K. Subczynski, T. Gregory Dewey and James G. Bann Geofrey E. Renk, Rosalie K. Crouch, James S. Hyde, and Akihiro Kusumi Proteins and Biopolymers Optical and physical properties of wet-spun films Muscle and Contractility of Na-hyaluronate. Evidence of a phase Evidence for structurally different attached states transition. M.R. Flowers, R. L. Marlowe, of myosin cross-bridges on actin during S. A. Lee, N. Lavalle, and A. Rupprecht contraction of fish muscle. Jeffrey J. Harford and Formation of a square-planar Co(l) B,. John M. Squire intermediate. Implications for enzyme catalysis. Myosin light chain 2 modulates calcium-sensitive Michael D. Wirt, Irit Sagi, and Mark R. Chance ... cross-bridge transitions in vertebrate skeletal Protein dynamics. Vibrational coupling, spectral muscle. Joseph M. Metzger and RichardL.Moss 460 broadening mechanisms, and anharmonicity Theory of excitation-contraction coupling in effects in carbonmonoxy heme proteins studied cardiac muscle. Michael D. Stern by the temperature dependence of the Soret band lineshape. Antonella Di Pace, Antonio Cupane, Maurizio Leone, Eugenio Vitrano, and Membranes Lorenzo Cordone Nonmonotonic alterations in the fluorescence 'H Nuclear magnetic resonance investigation of anisotropy of poiar head group labeled cobalt(II) substituted carbonic anhydrase. fluorophores during the lamellar to hexagonal Lucia Banci, L. B. Dugad, Gerd N. La Mar, phase transition of phospholipids. Xianlin Han Kelly A. Keating, Claudio Luchinat, and and Richard W. Gross Roberta Pierattelli Spin-labeling studies of the conformation of the Hydrogen peroxide plays a key role in the Ca?*-regulatory protein calmodulin in solution and oxidation reaction of myoglobin by molecular bound to the membrane skeleton in erythrocyte oxygen. A computer simulation. ghosts: Implications to transmembrane Tetsu-ichi Wazawa, Ariki Matsuoka, signaling. Mary Anne Yacko and Gen-ichi Tajima, Yoshiaki Sugawara, D. Allan Butiertield Ken-ichi Nakamura, and Keiji Shikama Formation of a new stable phase of Dimerization kinetics of the IgE-class antibodies phosphatidyiglycerols. Richard M. Epand, by divalent haptens. |. The Fab-hapten Brent Gabel, Raquel F. Epand, Arindam Sen, interactions. Reinhard Schweitzer-Stenner, Sek Wen Hui, Arturo Muga, and Arieh Licht, and Israel Pecht Witold K. Surewicz Contents Determination of fluid and gel domain sizes in Classification of projection images of crystalline two-component, two-phase lipid bilayers. An arrays of the mitochondrial, voltage-dependent electron spin resonance spin label study. anion-selective channel embedded in M. B. Sankaram, D. Marsh, and T. E. Thompson aurothioglucose. Xiao-Wei Guo and Influence of the membrane surface on glycolipid Carmen A. Mannella conformation and dynamics. An interpretation of Conformational model for ion permeation in NMR results using conformational energy membrane channels: A comparison with multi-ion calculations. BeAtrice G. Winsborrow, models and applications to calcium channel Jean-Robert Brisson, lan C. P. Smith, and permeability. Sergej L. Mironov Harold C. Jarrell 23Na and 'H NMR studies on melittin channels Direct correlation of structure changes and activated by tricyclic tranquilizers. Hisao Tanaka, thermal events in hydrated lipid established by Kohichi Matsunaga, and Hiroshi Kawazura simultaneous calorimetry and time-resolved x-ray Mode of action of iberiotoxin, a potent blocker of diffraction. Hesson Chung and Martin Caffrey ... the large conductance Ca?*-activated K* Intermediates and kinetics of membrane fusion. channel. Sebastian Candia, Maria L. Garcia, and Joe Bentz Ramon Latorre Melittin-induced changes in lipid multilayers. A solid-state NMR study. Ross Smith, Electrophysiology Frances Separovic, Fiona C. Bennett, and Techniques for obtaining analytical solutions to Bruce A. Cornell the multicylinder somatic shunt cable model for Spatial variations in membrane properties in the passive neurones. J. D. Evans, G. C. Kember, intact ratlens. G. J. Baldo and R. T. Mathias ... and G. Major Oxygenation-deoxygenation cycle of erythrocytes Analysis of electric field stimulation of single modulates submicron cell membrane cardiac muscle cells. Leslie Tung and fluctuations. Shmuel Tuvia, Shlomo Levin, and Jean-René Borderies Rafi Korenstein Magnetic and electric characteristics of the electric fish Gymnotus carap6. Oswaldo Baffa Channels and Sonia Lopes Cérrea Properties of the mitochondrial peptide-sensitive cationic channel studied in planar bilayers and patches of giant liposomes. Michel Thieffry, Jacques Neyton, Marc Pelleschi, Florence Févre, and Jean-Pierre Henry * Brief communication Biophysical Journal Volume 63 December 1992 biophysical journal september 1992 volume 63 number 3 contents Channels and Receptors flow immunofluorescence. Marjorie L. Longo, Dihydropyridine-sensitive skeletal muscle Ca Alan Waring, and Joseph A. N. Zasadzinski channels in polarized planar bilayers. 3. Effects of Conformation and elasticity of the isolated red phosphorylation by protein kinase C. Jianjie Ma, blood cell membrane skeleton. Karel Svoboda, Luis M. Gutiérrez, M. Marlene Hosey, and Christoph F. Schmidt, Daniel Branton, and Eduardo Rios Steven M. Block Na channels that remain open throughout the Quenching of fluorescein-conjugated lipids by cardiac action potential plateau. Yuan-mou Liu, antibodies. Quantitative recognition and binding Louis J. DeFelice, and Michele Mazzanti of lipid-bound hapiens in biomembrane models, A method for estimation of drug affinity constants formation of two-dimensional protein domains and to the open conformational state of calcium molecular dynamics simulations. M. Ahlers, channels. E. N. Timin and S. Hering D. W. Grainger, J. N. Herron, K. Lim, H. Ringsdorf, and C. Salesse Prolines are not essential residues in the ‘‘barrel- stave” model for ion channels induced by Muscie and Cell Motility alamethicin analogues. Hervé Duclohier, Gérard Molle, Jean-Yves Dugast, and Cooperativity of thiol-modified myosin filaments. Gérard Spach ATPase and motility assays of myosin function. Douglas D. Root and Emil Reisler Electrophysiology Quantitation of liquid-crystalline ordering in F-actin Pools of water in anhydrobiotic organisms. solutions. Chris M. Coppin and Paul C. Leavis 794 A thermally stimulated depolarization current Time-resolved x-ray diffraction study of the study. Fabio Bruni and A. Carl Leopold troponin-associated reflexions from the frog Simplified method for setting the phase angle for muscle. Yuichiro Maéda, David Popp, and use in Capacitance measurements in studies of Alexander A. Stewart exocytosis. Kenneth Zierler Nucleic Acids Membranes and Bilayers The Nucleic Acid Database. A comprehensive Modulation of lateral transport of membrane relational database of three-dimensional components by spatial variations in diffusivity and structures of nucleic acids. Helen M. Berman, solubility. Anthony de Beus and Josef Eisinger Wilma K. Olson, David L. Beveridge, Computer detection of the rapid diffusion of John Westbrook, Anke Gelbin, Tamas Demeny, fluorescent membrane fusion markers in images Shu-Hsin Hsieh, A. R. Srinivasan, and observed with video microscopy. Walter D. Niles, Bohdan Schneider Qing Li, and Fredric S. Cohen Further evidence for closed, nonspherical Photobiophysics aggregates in the cubic /, phase of lysolecithin and Kinetic analysis of the activation of transducin by water. Gdran Lindblom, Lennart B.-A. Johansson, photoexcited rhodopsin. Infiuence cf the lateral Goran Wikander, Per-Olof Eriksson, and diffusion of transducin and competition of Gosta Arvidson guanosine diphosphate and guanosine Lipid bilayer surface association of lung surfactant triphosphate for the nucleotide site. protein SP-B, amphipathic segment detected by Franz Bruckert, Marc Chabre, and T. Minh Vuong 616 Contents Proteins and Biopolymers heme is a planar oscillator. Zygmunt Gryczynski, Todd Tenenholz, and Enrico Bucci Direct observation of defect structure in protein crystals by atomic force and transmission electron Investigation of the structural determinants of the microscopy. Genevieve Devaud, intrinsic fluorescence emission of the trp repressor Paul S. Furcinitti, John C. Fleming, using single tryptophan mutants. Mary K. Lyon, and Kenneth Douglas Catherine A. Royer Quaternary structure and geminate recombination Conformational dynamics and intersubunit energy in hemoglobin: flow-flash studies on a$°8, and transfer in wild-type and mutant lipoamide a85°. Debkumar Bandyopadhyay, dehydrogenase from Azotobacter vinelandii. Douglas Magde, T. G. Traylor, and A multidimensional time-resolved polarized Vijay S. Sharma fluorescence study. Philippe |. H. Bastiaens, Arie van Hoek, Jacques A. E. Benen, Strong electrostatic loop-helix interactions in Jean-Claude Brochon, and bundle motif protein structures. Kuo-Chen Chou Antonie J. W. G. Visser and Chong Zheng Vibrationally enhanced tunneling as a mechanism Cell Surface Phenomena for enzymatic hydrogen transfer. William J. Bruno and William Bialek How do selectins mediate leukocyte rolling in venules? Aydin Toézeren and Klaus Ley Influence of sickle hemoglobin polymerization and Interrupting autocrine ligand-receptor binding: membrane properties on deformability of sickle erythrocytes in the microcirculation. comparison between receptor blockers and ligand Cheng Dong, Richard S. Chadwick, and decoys. Kimberly E. Forsten and Alan N. Schechter Douglas A. Lauffenburger Penetration of macromolecules into contracted Cell Biophysics blood clot. Maxim Yu. Matveyev and Serguej P. Domogatsky Cytoplasmic calcium stimulates exocytosis in a plant secretory cell. Robert Zorec and Fluorescence Mark Tester Rates of energy transfer between tryptophans and hemes in hemoglobin, assuming that the * Brief communication Biophysical Journal Volume 63 December 1992 biophysical journal october 1992 volume 63 number 4 contents SSOE NES TREE: Biophysical Theory Molecular origin of the internal dipole potential in lipid bilayers: calculation of the electrostatic Confinement as a determinant of macromolecular potential. Chong Zheng and Garret Vanderkooi structure and reactivity. Allen P. Minton Effects of the anesthetic dibucaine on the kinetics of the gel-liquid crystalline transition of Channels dipalmitoyiphosphatidyicholine multilameliar Limitations of the dual voltage clamp method in vesicies. William W. van Osdol, Qiang Ye, assaying conductance and kinetics of gap junction Michael L. Johnson, and Rodney L. Biltonen channels. Ronald Wilders and Habo J. Jongsma 942 Study of mechanisms of electric field-induced Intra and extracellular surface charges near Ca** DNA transfection IV. Effects of DNA topology on channels in neurons and neuroblastoma cells. cell uptake and transfection efficiency. T. D. Xie, Andrea Becchetti, Annarosa Arcangeli, L. Sun, H. G. Zhao, J. A. Fuchs, and Tian Y. Tsong 1026 Maria Riccarda Del Bene, Massimo Olivotto, Acyl chain conformational ordering in 1,2 and Enzo Wanke dipalmitoylphosphatidylethanolamine. Integration Coupling of water and potassium ions in K* of FT-IR and 2H NMR results. Mark Allen Davies, channels of the tonoplast of Chara. Wigand Hubner, Alfred Blume, Fabrice Homblé and Anne Aliénor Véry and Richard Mendelsohn A-type potassium channel clusters revealed using Adenine nucleotide translocase greatly increases a new statistical analysis of loose patch data. the partition of trinitrophenyl-ATP into reduced Samuel S.-H. Wang and Stuart Thompson Triton X-100 micelles. Peter J. Tummino Shaking stack model of ion conduction through and Ari Gafni the Ca**-activated K* channel. Cholesterol in model membranes. A molecular Mark F. Schumaker dynamics simulation. Olle Edholm and Anna M. Nyberg Electrophysiology The exocytotic fusion pore modeled asa lipidic Effect of firing rate on the calcium permeability in pore. C. Nanavati, V. S. Markin, adult neurons during spontaneous action A. F. Oberhauser, and J. M. Fernandez potentials. Michele Mazzanti, Aurelio Galli, and Fatty-acid chain tilt angles and directions in Arnaldo Ferroni dipalmitoyl phosphatidylcholine bilayers. John Katsaras, Daniel S-C. Yang, Evaluation of cellular mechanisms for modulation of calcium transients using a mathematical model and Richard M. Epand of fura-2 Ca?* imaging in Aplysia sensory Phosphatidyicholine: cholesterol phase neurons. Hal Blumenfeld, Leonard Zablow, and diagrams. Jenifer L. Thewalt and Myer Bloom Bernardo Sabatini Membranes and Bilayers Membranes Interfacial models of nerve fiber cytoskeleton. Hydration at the membrane protein-lipid V. V. Malev, D. B. Gromov, Ya. Yu. Komissarchik, interface. Cojen Ho and Christopher D. Stubbs and M. S. Brudnaya E/M dips. Evidence for lipids regularly distributed Muscle and Cell Motility into hexagonal super-lattices in pyrene-PC/DMPC binary mixtures at specific concentrations. Ciliary motion modeling, and dynamic multicilia Daxin Tang and Parkson Lee-Gau Chong interactions. Shay Gueron and Nadav Liron .... Contents Muscle and Contractility Proteins Orientational distribution of spin-labeled actin Rotational diffusion and intermolecular collisions oriented by flow. &. Michael Ostap, of a spin labeled a-helical peptide determined by Toshio Yanagida, and David D. Thomas electron spin echo spectroscopy. Time-resolved tryptophan emission study of Siobhan M. Miick and Glenn L. Millhauser cardiac troponin |. Ronglih Liao, Chein-Kao Polarization sensitive coherent anti-Stokes Raman Wang, and Herbert C. Cheung scattering spectroscopy of the amide | band of Studies on the structure of actin gels using time proteins in solutions. Andrey Yu. Chikishev, correlation spectroscopy of fluorescent beads. Gerald W. Lucassen, Nikolai |. Koroteev, Hong Qian, Elliot L. Elson, and Carl Frieden 1000 Cees Otto, and Jan Greve A mosaic multiple-binding model for the binding of Proteins and Biopolymers caldesmon and myosin subfragment-1 to actin. Yi-der Chen and Joseph M. Chalovich Measuring the microelastic properties of biological material. N. J. Tao, S. M. Lindsay, and S. Lees Photobiophysics Supramolecular Assemblies Kinetic model of primary energy transfer and lon channels in icosahedral virus: a comparative trapping in photosynthetic membranes. analysis of the structures and binding sites at their T6nu Pullerits and Arvi Freiberg fivefold axes. Susana G. Kalko, Raul E. Cachau, On the long-wavelength component of the light- and Abelardo M. Silva harvesting complex of some photosynthetic bacteria. Monika R. Fischer and Arnold J. Hoff . . * Brief communication Biophysica! Journal Volume 63 December 1992