1 ASIAN-PACIFIC AQUACULTURE 2018 Innovation For Aquaculture Sustainability and Food Safety Taipei International Convention Center April 23-26, 2018 • Taipei, Taiwan Organized by: World Aquaculture Society - Asian Pacific Chapter Hosted by: National Taiwan Ocean University Gold Sponsor Conference Sponsors 2 Welcome to Asian Pacific Aquaculture 2018 Dear delegates and guests, On behalf of the Asia-Pacific Chapter of the World Aquaculture Society, I would like to extend to you a warm welcome to #APA18. For our annual meeting in Taipei this year, we have decided to join forces with the National Taiwan Ocean University, selecting “Innovation for Aquaculture Sustainability and Food Safety” as our conference title. Collaborating with NTOU was an obvious choice given their importance in the Taiwanese education landscape; also, the timing is perfectly in line with increased WAS activities and visibility in Taiwan. We expect a large number of new members from a Chinese speaking background – not only from Taiwan, but also from all around Asia-Pacific. The conference title has been chosen with care by the Steering Committee, and it succinctly reflects both the needs and the changes already taking place in our industry. I firmly believe that innovation will drive most of the upcoming changes not only in our industry but also in education programs in the decades to come. Do we have any choice but to embrace new ways of working to adapt to a rapidly changing world? Do we have any choice but to integrate new technologies and adjust our business models to adhere to increasingly scientifically based and stringent regulations? We are constrained by limited natural resources, yet we must produce more to feed the ever-expanding global population. Our industry must support the transformation of food production systems such that hunger can be eradicated globally as preconized by the UN Sustainable Development Goals. Our farms must ensure that ecosystems are protected to allow long-term prosperity of aquatic ecosystems. Our private and public stakeholders must ensure that the products we grow are of high quality and are tested rigorously to safeguard the consumer’s health. Our conference focus might be on sustainability and food safety, but advancements in other fields of aquaculture will not be neglected – for example breeding, feed formulation, and species specific technical presentations. I am convinced we will have an outstanding conference, and I would take this opportunity to express my utmost appreciation to our conference and program managers who have been working hard to ensure a well-organised event. I am also confident that the tradeshow will once again be a tremendous success for all companies showcasing their new products to the industry. There is no doubt that technology adoption and integration will be the key to sustainability and progress of our industry. This conference will serve as a platform for like-minded professionals to interact, exchange valuable ideas and gain perspective of the multifaceted challenges our industry is facing. My final words will go out to our future members of the World Aquaculture Society. Enjoy the excellent Taiwanese hospitality and take your time to learn more about us. Together with the board, we look forward to seeing you join our team and supporting the development of aquaculture in the Asia-Pacific region! I wish you a fruitful and successful conference in Taiwan! Dr. Ching-Fong Chang & Dr. Guillaume Drillet Co-Chairs 3 TABLE OF CONTENTS Welcome ...................................................................... 2 Asian-Pacific Aquaculture 2018 Abstracts .......... 5 To find abstracts for a specific author or subject, use the pdf search features built into Adobe Acrobat. DISCLAIMER ASIAN-PACIFIC AQUACULTURE 2018 prints abstracts in this Abstract Book exactly as they are submitted without editing or confirmation of material contained in the abstract. ASIAN-PACIFIC AQUACULTURE 2018 has no responsibility for the information contained in the abstracts. ASIAN- PACIFIC AQUACULTURE 2018is not responsible for authors or contact information contained in the abstracts. ASIAN-PACIFIC AQUACULTURE 2018 does not have any liability for problems or damages caused by the use of the information in the abstracts published in the Abstract Book. Any one using these abstracts needs to verify the information in the abstracts on their own before utilizing such information and will have full liability for results of using such information. 4 ABSTRACTS 5 DIETARY SUPPLEMENTATION OF LOCAL MEDICINAL HERBS FOR HYBRID GROUPER Epinephelus fuscoguttatus X E. lanceolatus JUVENILE Dayang Nur Jazlyn Abang Zamhari* and Annita Yong Seok Kian Borneo Marine Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia [email protected] Use of herbs in aquaculture is getting popular nowadays. Malaysia is rich in many traditional herbs in which its full potential has not been fully discovered yet. Therefore, present study was conducted to screen some of the local medicinal herbs and introducing them in the feed for hybrid grouper juvenile. Methanolic extracts of four fresh local medicinal herbs, betel (Piper betle), turmeric (Curcuma longa) and “tuhau” (Etlingera coccinea) were screened for antioxidant and antimicrobial activities in-vitro . Antioxidant activity was tested using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging activity while disc diffusion for antibacterial and fungus block dipping for antifungal activity. Four Vibrio species were used for antibacterial activity while Fusarium moniliforme was used for antifungal activity. Results from the screening showed that betel extract provided best results for all tested activities followed by turmeric and tuhau. Thus, a feeding trial was then conducted to investigate the effects of herbs supplementation in the diets on the growth, feed utilization and apparent digestibility coefficient of hybrid grouper juvenile for 8 weeks. Fish with initial body weight of 8.91 ± 0.02g were fed with experimental diets supplemented with 0.5% herbs which are D-betel, D-turmeric, D-tuhau. Experimental diet without supplementation of herbs was served as control. Fish were fed until apparent satiation level twice a day. Results showed highest growth in fish fed D-turmeric, followed by D-tuhau while fish fed D-betel showed the lowest growth. However, the growth of fish fed D-turmeric and D-tuhau supplementation has no significant difference than the fish fed with the control diet (p > 0.05) (Table 1). Better feed utilization was also observed in fish fed D-turmeric and D-tuhau compared to D-betel. Supplementation of turmeric and tuhau improved the fish’s body condition and body composition however there were no significant difference than the control (p > 0.05). The apparent digestibility coefficient (ADC) of protein and lipid of fish fed D-turmeric and D-tuhau were significantly higher than the control (p < 0.05). Meanwhile, fish fed D-betel showed lower ADC of protein and lipid suggests that presence of betel perhaps its active compounds or inclusion level may interrupt the nutrient digestion and absorption of the fish. Overall, the supplementation of turmeric and tuhau gave positive effects in terms of growth, feed utilization and apparent digestibility coefficient to the fish in all tested parameters. 6 DIETARY INFLUENCE OF YEAST AS A SOURCE OF NUCLEOTIDES ON TILAPIA Oreochromis niloticus Nermeen M. Abu-Elala*, Nehal A. Younis, Huda O. AbuBakr, Melina A. Bonato, Liliana L. Borges Department of Fish Diseases and Management, Faculty of Veterinary Medicine Cairo University Giza 12211, Cairo, Egypt [email protected] The objective of this study was to evaluate the effect of yeast as nucleotide source on growth performance, oxidative stress, immune response, expression of some immune related genes and mortality of Tilapia (Oreochromis niloticus) challenged with Aeromonas hydrophila and Lacococcus gravaeie. A total of 270 O. niloticus (50.7±0.8 g of BW) were distributed in a completely randomized design, with 3 treatments: Control; 0.2% of Saccharomyces cerevisiae as a source of nucleotides (YNU - Hilyses®, ICC Brazil); 0.2% of YNU, with 90 fish each treatment (three replicate/tanks). The fishes were fed with 3% of its total biomass during 3 months; the acclimation period was 2 weeks. The growth parameters measured were body weight (BW, g/ind.), body weight gain (BWG, g/ind.) and feed conversion ratio (FCR) at 1 and 2 months. Also the clinicophatological, oxidant parameters, relative quantitative PCR of immune gene expression, phagocytic activity (%) and index, lysozyme activity (µg/mL) were evaluated at 2 months. After 2 months, the fishes were challenged against Gram positive bacteria L. gravaeie and Gram negative A. hydrophila and the mortality rates were observed during 1 week. The data were analyzed by Tukey test (p≤0.05) using SPSS version 21. YNU supplementation improved the growth performance in relation the control group (P<0.05). Similary, YNU in both levels increased catalase and G-redutase activities, and expression of immune related genes parameters (P<0.05). Also, the phagocytic and lysozyme activity were increased (P<0.05). After the challenge with the both bacteria’s, mortality decreased (P<0.05) in both groups with YNU (Table 1). In general, the inclusions of 0.2 and 0.4% of YNU have proven to improve the growth performance of Nile tilapia, boost the immune response and increase the resistance against diseases. 7 DIETARY INFLUENCE OF YEAST CELL WALL ON THE GROWTH PERFORMANCE, OXIDATIVE STRESS PARAMETERS AND IMMUNITY OF CULTURED Oreochromis niloticus Nermeen M. Abu-Elala*, Nehal A. Younis, Huda O. AbuBakr, Melina A. Bonato, Liliana L. Borges Department of Fish Diseases and Management, Faculty of Veterinary Medicine Cairo University Giza 12211, Cairo, Egypt [email protected] To evaluate the effect of dietary supplement (Immunowall®) on growth performance, clinicopathological parameters, oxidative stress, expression of some immune related genes, innate immunity and mortality of Tilapia (Oreochromis niloticus) challenged with Aeromonas hydrophila and Lacococcus gravaeie. A total of 270 O. niloticus (50.7±0.8 g of BW) were distributed in a completely randomized design with 3 treatments: Control; 0.1% of Saccharomyces cerevisiae yeast cell wall (YCW- ImmunoWall®, ICC Brazil); 0.2% of YCW, with 90 fish each treatment (three replicate/tanks). The body weight (BW, g/ind.), body weight gain (BWG, g/ind.) and feed conversion ratio (FCR) at 1 and 2 months were measured. Besides, the clinicophatological, oxidant parameters, relative quantitative PCR of immune gene expression, phagocytic activity (%) and index, lysozyme activity (µg/mL) were evaluated at 2 months. After 2 months, the fishes were challenged against L. gravaeie and A. hydrophila and the mortality rates were observed during 1 week. The data were analyzed by Tukey test (p≤0.05) using SPSS version 21. There was no significant difference (P>0.05) among treatments for performance results. However, YCW improved clinicophatological results as well as, PCR expression of IL1-β, phagocytic and lysozyme activities (P<0.05). There was the reduced of G-redutase enzymatic activities and 0.2% of YCW supplementation increased catalase enzyme. After the challenge with the both bacteria’s, mortality decreased (P<0.05) in both groups with YCW. The inclusions of 0.1 and 0.2% of YCW were able to improve clinicophatological response and innate immunity. YCW supplementation decreased oxidative enzymes activity and mortality rates when the fishes were challenged. 8 AN INSIGHT INTO THE AQUACULTURE SECTOR MANAGEMENT IN NIGERIA Adi Uchenna Michael*, Xiongzhi Xue Coastal and Ocean Management Institute Xiamen University Xiamen, Fujian Province, 361102 +8613159229582, [email protected] Aquaculture is no doubt a characteristic homogenous to coastal states and have over the years become a vital aspect in planning towards sustainability and economic development of coastal states in particular. Nigeria being a country with a wealth of resources (biotic and abiotic) within its inland, coastal and offshore regions has not only drawn utility from these resources but have also had a fair amount of daunting challenges arising from a questions on how, why, where and who is to take responsibility for effective management of this resources as these challenges overlap different institutions in the country. This study aims to know the key aquaculture management institutions in Nigeria for the past five (5) years, their operational framework, their jurisdiction of authority, administrative challenges, administrative successes, lags in the management of the this sector and answer the question of why effective sustainable management should be adopted especially for the aquaculture sector which has shown remarkable potentials in recent times. 9 PREVALENCE, RISK FACTORS AND TRANSMISSION OF VIRAL NERVOUS NECROSIS IN A HATCHERY PRODUCING HYBRID GROUPER FRY Atirah K.A.1, Amal M.N.A.1,2,*, Zamri-Saad M.2,3, Azila A.4, Sufian M.5, Nik-Haiha N.Y.5 1 Laboratory of Marine Biotechnology, Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia 2 Department of Biology, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. 3 Department of Veterinary Laboratory Diagnosis, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia. 4 National Fish Health Research Centre, Fisheries Research Institute, 11900 Batu Maung, Penang, Malaysia. 5 Marine Fish Aquaculture Research Division, Fisheries Research Institute, Tanjung Demong, 22200 Besut, Terengganu, Malaysia. *[email protected] Grouper is one of the high value and demanded cultured marine fish especially in the Southeast Asia countries. However, the supply of groupers from aquaculture is often limited due to betanodavirus. This study investigates the prevalence, f risk factors and transmission of viral nervous necrosis (VNN) in a hatchery producing hybrid grouper ( giant grouper m Epinephelus lanceolatus x tiger grouper E. fuscoguttatus) fry. The experiment was conducted in a marine fish hatchery located in Terengganu, Malaysia. The eggs and sperm of giant grouper (GG) and tiger grouper (TG) brood stocks, respectively, were taken for VNN detection using PCR. After fertilization, the fry were observed for VNN status at 5, 10, 20, 30, 40, 60, 90 and 120 days post hatching, while the water quality during the study period were recorded. The detected VNN were proceed for molecular characterization. The prevalence of VNN in brood stocks throughout 12 months of sampling showed low detection of VNN. VNN only detected in April 2016 with a percentage of 50% for GG and 33.33% for TG brood stocks. However, there were correlations between the presence of VNN in GG, TG and hybrid grouper (HG) juveniles with water quality parameters. Moreover, iron and ammonia consistently showed significant relationship with the presence of VNN in GG, TG and HG. VNN transmission study and molecular characterization analysis suggested possible horizontal and vertical transmission of VNN between the groupers in this hatchery (Table 1). This study provides significant information for control and prevention of VNN to farmers in this country that producing HG fry at hatchery level. 10 GROWTH RATE OF Kappaphycus striatum var cottonii AT DIFFERENT POSITIONS OF THE CULTIVATION LINES TO THE DIRECTION OF WATER CURRENT Jainal P. Akrim*, Didang Shara U. Jumah, Rafsanjani M. Samimi, Cherry T. Nian Aquaculture Department, College of Fisheries Mindanao State University-Tawi-Tawi College of Technology and Oceanography Sanga-Sanga, Bongao, Tawi-Tawi, Philippines [email protected] The study was conducted to determine the effects of different positions in cultivation lines towards the direction of water current on growth rate of Kappaphycus striatum var. cottonii for a duration of 45 days at 04°30’936”N and 119°22’068”E of Bungin Pondohan, Sitangkai, Tawi-Tawi. The experiment has three treatments; the parallel (T1), perpendicular (T2) and diagonal (T3) positions towards the direction of water current with 5 replicates in each treatment following the randomized complete block design (RCBD). Results showed that higher mean weight of 473 g and growth increment of 8.3 g/day were obtained from diagonal positions (T3), although not significantly different from mean weights and growth increments parallel (T1) and perpendicular (T2) of 431 g and 7.4 g/day, and 408 g and 6.8 g/day, respectively. However, results showed statistically similar growth rates of Kappaphycus striatum var cottonii among all treatments. Thus, the positions of cultivation lines with respect to the direction of the current have no significant effect on the growth of the said seaweed species. Hence, the treatments used in this study are viable in growing Kappaphycus striatum var. cottonii in the selected culture site.