Ascough, P. L., Church, M. J., Cook, G. T., Einarsson, Á., McGovern, T. H., Dugmore, A. J., and Edwards, K. J. (2014) Stable isotopic (δ13C and δ15N) characterization of key faunal resources 1 from Norse period 2 settlements in North Iceland. Journal of the North Atlantic, 7(7). pp. 25-42. Copyright © 2014 Eagle Hill Publications A copy can be downloaded for personal non-commercial research or study, without prior permission or charge Content must not be changed in any way or reproduced in any format or medium without the formal permission of the copyright holder(s) When referring to this work, full bibliographic details must be given http://eprints.gla.ac.uk/95036 Deposited on: 20 February 2015 Enlighten – Research publications by members of the University of Glasgow http://eprints.gla.ac.uk 1 Stable isotopic (δ13C and δ15N) characterization of key faunal resources from Norse period 2 settlements in North Iceland 3 4 Philippa L. Ascough1,*, Mike J. Church2, Gordon T. Cook1, Árni Einarsson3, Thomas H. 5 McGovern4, Andrew J. Dugmore5, Kevin J. Edwards6,7. 6 7 1SUERC, Scottish Enterprise Technology Park, Rankine Avenue, East Kilbride G75 0QF, UK 8 2Department of Archaeology, Durham University, South Road, Durham DH1 3LE, UK. 9 3 Mývatn Research Station, Skútustaðir, Iceland and Institute of Biology and Environmental Sciences, University of 10 Iceland, Reykjavik, Iceland. 11 4Hunter Bioarchaeology Laboratory, Hunter College CUNY, NYC 10021, USA 12 5Institute of Geography, School of GeoSciences, University of Edinburgh, Drummond Street, Edinburgh EH9 8XP, UK 13 6Departments of Geography & Environment and Archaeology, University of Aberdeen, Elphinstone Road, Aberdeen 14 AB24 3UF, UK 15 7St Catherine’s College, University of Oxford, Manor Road, Oxford OX1 3UJ, UK 16 *corresponding author email: [email protected] 17 18 Abstract: 19 During the Viking Age, Norse peoples established settlements across the North Atlantic, colonizing 20 the pristine and near-pristine landscapes of the Faroe Islands, Iceland, Greenland and the short-lived 21 Vinland settlement in Newfoundland. Current North Atlantic archaeological research themes 22 include efforts to understand human adaptation and impact in these environments. For example, 23 early Icelandic settlements persisted despite substantial environmental impacts and climatic change, 24 while the Greenlandic settlements were abandoned ca. AD 1450 in the face of similar 25 environmental degradation. The Norse settlers utilized both imported domestic livestock and natural 26 fauna, including wild birds and aquatic resources. The stable isotope ratios of carbon and nitrogen 27 (expressed as δ13C and δ15N) in archaeofaunal bones provide a powerful tool for the reconstruction 28 of Norse economy and diet. Here we assess the δ13C and δ15N values of faunal and floral samples 29 from sites in North Iceland within the context of Norse economic strategies. These strategies had a 30 dramatic effect upon the ecology and environment of the North Atlantic islands, with impacts 31 enduring to the present day. 32 33 Keywords: Stable isotopes, Iceland, North Atlantic, zooarchaeology, diet reconstruction 34 1 Introduction 2 The Viking settlement of the North Atlantic commenced around AD 800, and was characterized 3 by rapid expansion of the Norse over a wide geographical area, including Scotland, the Faroe 4 Islands, Iceland, and Greenland (e.g. Sharples and Parker Pearson 1999, Vésteinsson et al. 2002, 5 Arge et al. 2005, Dugmore et al. 2005). In a relatively short time, settlements were established in a 6 broad set of ecological and climatic zones, and agriculture was established in many previously 7 pristine environments (Vésteinsson 1998, Dugmore et al. 2005, McGovern et al. 2007). Macro-scale 8 settlement outcomes varied markedly, from long-term sustainability in the Faroes and Iceland, to 9 abandonment of Greenlandic settlements in the mid 15th century AD (Dugmore et al. 2007a, 2012). 10 This variation is also evident on smaller geographical scales; in Iceland the overall continuity of 11 settlement is overlain by differences in the history and longevity of individual farm sites (Dugmore 12 et al. 2007b). Understanding the mechanisms for this variation is a key component in the 13 reconstruction of Viking histories in the North Atlantic, but this aim is frequently confounded by 14 the complexity of social, economic and environmental interactions that influenced the behaviour of 15 inhabitants at a site. 16 One recurring and crucial research question is: what economic strategy was in place at a 17 particular settlement? Understanding economic practices, particularly in terms of diet and animal 18 husbandry, is essential to the reconstruction of human-environment interactions. Over recent years, 19 the utility of stable isotope analysis in this regard has become increasingly apparent (e.g. Ambrose 20 1986, Schwarcz and Schoeninger 1991, Arneborg et al. 1999, Richards and Hedges 1999, Barrett 21 and Richards 2007, Richards et al. 2006, Ascough et al. 2012; Arneborg et al., 2012). In this study, 22 we investigate the use of stable isotope ratios of carbon and nitrogen, expressed as δ13C and δ15N, as 23 a tool to reconstruct economic practice at early Viking period sites within the region of 24 Mývatnssveit, northern Iceland (Fig. 8.1). 25 Norse North Atlantic communities used both agricultural and wild resources to build a broad- 26 spectrum, effective and flexible subsistence system that was initially based on traditional economic 27 knowledge from the Norse homelands and then adapted to local settings (Dugmore et al. 2005, 28 2012). The agricultural component involved cows, sheep, goats, pigs, horses and dogs, plus, where 29 possible, arable agriculture. The wild component varied but could include freshwater and marine 30 fish, birds, and marine mammals. Individual farms generally operated as part of a multi-farm 31 cooperative system, involving exchange of materials and products with communal management of 32 practices, such as upland grazing. The economic system was not static but responded to changing 33 environmental conditions and social pressures. 1 Measurements of δ13C and δ15N are a valuable tool in archaeological palaeodietary 2 reconstruction. These measurements represent an integration of δ13C and δ15N isotope values in 3 food consumed over the time a tissue (e.g. bone collagen) was formed (Tieszen 1978, Hobson and 4 Clark 1992, Hedges et al. 2007). There is also a diet-tissue offset, meaning that δ13C and δ15N 5 increase within an organism with each trophic level up a food chain by typically ~1-2‰ for δ13C, 6 and 3-5‰ for δ15N. An increase in trophic level has also been observed in the δ15N of neonatal and 7 suckling animals relative to the tissues of the mother in both modern and archaeological populations 8 (e.g. Fuller et al. 2006, Ascough et al. 2012). Although the typical source-consumer δ13C offset is 9 minimal, it should be noted that the bone collagen diet-tissue δ13C offset appears to show species 10 and diet-dependant variations (e.g. Hare et al., 1991), with a recent survey suggesting an offset of 11 +3.6‰ for mammalian collagen (Szpak et al. 2012b). If the isotopic values of possible dietary 12 components are sufficiently different, then the proportion of each component that was consumed by 13 an organism can be assessed by analysis of its body tissues. δ13C and δ15N measurements of 14 archaeological samples are usually made using bone collagen and have proved particularly useful in 15 discriminating between terrestrial and marine components in the diet of human populations, as there 16 is a large and consistent difference between both carbon and nitrogen isotope values in marine and 17 terrestrial organisms (Arneborg et al. 1999, Richards et al. 2006, Sveinbjörnsdóttir et al. 2010). 18 Commonly, this involves modelling the proportion of different theoretical dietary components. The 19 accuracy of such isotope-based diet reconstruction depends heavily on how accurately the source 20 isotopic compositions for each resource group represent the resources actually consumed. This 21 means that the selection of appropriate end-member values for such a model is critical (Dewar and 22 Pfeiffer 2010). Importantly, both the resources included in the economic strategy of the inhabitants 23 of the archaeological site and the isotope values of these resources, must be known. 24 Values of δ13C and δ15N show wide geographical variation, meaning that the values for a 25 species in one region cannot necessarily be used in palaeodietary reconstruction for another region. 26 Geographic variations occur due to a range of environmental and anthropogenic variables, 27 summarised in Rubenstein and Hobson (2004). Terrestrial δ13C decreases with increasing latitude 28 and increases with altitude due to temperature effects, while in C plant-based ecosystems, dry 3 29 habitats are enriched in δ13C compared to wet habitats due to differences in water use efficiency 30 (Lajtha and Marshall 1994). In marine environments, δ13C decreases with latitude, leading to 31 northern oceans being enriched in δ13C compared to southern oceans and benthic systems are 32 enriched in δ13C compared to pelagic systems. These effects are ascribed to temperature 33 differences, surface-water CO concentration offsets and differences in plankton biosynthesis or 2 34 metabolism (Kelly 2000). Terrestrial plant tissue δ15N varies according to the method of nitrogen 1 fixation, the influence of anthropogenically and naturally added fertilizers, land-use practices 2 resulting in differential loss of 14N and the enrichment of wet habitats in δ15N relative to dry 3 habitats (Kelly 2000). Marine δ15N geographic patterns are less well understood, although δ15N in 4 northern oceans appears more enriched compared to southern oceans (Kelly 2000). In addition to 5 the above variables, the isotope values of any resource (e.g., cattle) at a single location will show 6 considerable variability due to factors such as individual feeding preferences, age, sex or illness 7 (Hobson and Schwartz 1986, Hobson 1999, Bocherens and Drucker 2003). 8 This paper compiles stable isotope (δ13C and δ15N) values for a range of resources available to 9 early Norse settlements in northern Iceland, within the region of Mývatnssveit, surrounding Lake 10 Mývatn (Figure 8.1). This includes both domestic animals and wild resources, from four 11 archaeological sites: Undir Sandmúla (McGovern 2005), Sveigakot (Vésteinsson 2002), Hofstaðir 12 (Lucas 2010) and Hrísheimar (Edvardsson and McGovern 2007). The region has been the focus of 13 an international research effort to investigate human-environment interaction over the past twenty 14 years (McGovern et al. 2007). The dataset presented here includes the first investigation of 15 archaeological bird bone δ13C and δ15N for the study region. This is significant, given the extensive 16 evidence for exploitation of bird populations surrounding Mývatn by the Norse inhabitants of 17 Mývatnssveit (McGovern et al. 2007). In addition, analysis of bird remains from archaeological and 18 paleontological contexts have contributed significantly to better understanding of the ecology of a 19 number of bird species (e.g. Chamberlain et al. 2005, Fox-Dobbs et al. 2006, Emslie et al. 2007), 20 meaning the results may have value beyond archaeological investigations. 21 The aim of the research is firstly to compile a new and more comprehensive assessment of the 22 isotope values and their ranges for resources used in the Norse economy of the study area. 23 Secondly, it aims to investigate the potential for using isotope analysis of archaeofaunal remains in 24 informing researchers about animal husbandry practices in the study area. Animal husbandry is a 25 key component within North Atlantic archaeology but little research has addressed the direct 26 reconstruction of animal diet through stable isotope analysis. This paper therefore assesses the 27 isotopic values of archaeofauna from sites in Mývatnssveit to determine whether it is possible to use 28 these data to detect differences in husbandry practices in differing environments and between sites 29 of differing status or function. In omnivores, such as pigs, both δ13C and δ15N can vary significantly 30 between animals obtaining nutrients through free-range pannage, versus those that are stalled and 31 fed upon domestic waste including animal protein. This is particularly evident if the domestic waste 32 includes marine or freshwater resources. In herbivores, δ13C values tend to show less variability in 33 areas where plant communities are dominated by C vegetation (as in Iceland). However, plant δ15N 3 34 values can vary widely, depending upon local environment. Of particular interest to the current 1 study is that long-term intensive use of animal manure distinctly raises plant δ15N values relative to 2 unmanured areas (Bol et al. 2005, Commisso and Nelson 2006, 2007, Bogaard et al. 2007, Fraser 3 et al. 2011, Kanstrup et al. 2011, 2012). This elevation is considerable and has been shown to be as 4 high as 10(cid:125) in cereal grains (Kanstrup et al. 2012). High δ15N values in domestic animals may 5 therefore indicate enhancement of production via manuring practices or feeding of stalled animals 6 over winter. It is important to note that natural variation in plant δ15N values can also be 7 considerable and baseline values are required. The data presented here also include values of 8 modern vegetation from zones unaffected by modern agriculture in Mývatnssveit. 1 1. Methods 2 1.1. Sample material 3 Modern sample material 4 Stable isotope values used in this study represent the δ13C and δ15N of both modern and 5 archaeological biota from Mývatnssveit. These values include a range of new analyses and 6 previously published measurements. Modern vegetation was obtained from four locations close to 7 Mývatn (Haganes, Kálfaströnd, Framengjar and Hrúteyjarnes) and from two locations ca. 5 km 8 from the lake in the vicinity of the archaeological site of Sveigakot (Sveigakot and Seljahjallagil – 9 see Figure 8.1). At Framengjar and Hrúteyjarnes, multiple vegetation samples were collected along 10 a short transect to assess isotope variability in terrestrial plants at these locations in more detail. 11 Leaves were sampled from living vegetation, air-dried at 30 °C, followed by freeze-drying. Samples 12 were stored in pre-cleaned glass vials or plastic bags prior to subsequent analysis. Living biota from 13 within and around Mývatn, including freshwater fish, were obtained as described in Ascough et al. 14 (2010). Wildfowl were procured from local gyrfalcon (Falco rusticolus) nests, or from gillnets in 15 Lake Mývatn. Some were collected as roadkill adjacent to Mývatn as soon as practical after death. 16 Full sample details are given in Tables 8.1, 8.2 and 8.3. 17 18 Archaeofaunal sample material 19 The dataset of Norse period archaeofauna included in this study were obtained from four 20 sites of varying status in the Mývatnssveit region. Broadly, Hofstaðir is interpreted as a high-status 21 farm, while specialist activities, such as industry, appear to have taken place at the farms of 22 Hrísheimar and Undir Sandmúla. Finally, Sveigakot represents a lower-status farm site. The 23 holdings at Hofstaðir, Hrísheimar and Sveigakot are located at 250-350 meters above sea level, 24 while the territory of Undir Sandmúla is located slightly higher, at ~ 400 m a.s.l. All samples 25 retrieved date to the 9th to 11th centuries AD. The age of samples obtained was established through a 26 combination of tephrochronology and radiocarbon (14C) dating. Archaeofaunal samples included in 27 the dataset are the bones of domesticated mammals (cow, sheep, goat, pig, horse and dog) and wild 28 species (birds and freshwater fish). These materials were obtained during excavations for two main 29 projects: the Leverhulme Trust-funded “Landscapes circum-landnám” (Edwards et al. 2004), and 30 the NSF-funded “Long Term Human Ecodynamics in the Norse North Atlantic: cases of 31 sustainability, survival, and collapse” (McGovern 2011). Full sample details are given in Tables 8.1 32 and 8.2. 33 34 1.2. Laboratory methods 1 Pretreatment of dried vegetation involved homogenization of each sample by grinding using an 2 agate mortar and pestle. A sub-sample (c. 2-3 mg) of the ground material was then taken for 3 analysis. Bone samples of modern organisms were de-fatted prior to collagen extraction by 4 extraction with 2:1 (v/v) chloroform/methanol solution, followed by sonication for 60 minutes. The 5 extraction was repeated until the solvent remained clear. Collagen was extracted from bone samples 6 according to a modified Longin (1971) method. The sample surface was cleaned by abrasion with a 7 Dremmel® tool, after which the bone was crushed and placed in 1M HCl at room temperature 8 (~20°C). The bone was left in the HCl for up to 96 hours, after which dissolution of the bone 9 mineral component was complete. The solution was then decanted and the collagen washed in 10 reverse osmosis water. The collagen was placed in reverse osmosis water and the solution pH 11 adjusted to 3.0 by addition of 0.5 M HCl. The collagen was solubilized by gentle heating at ~80ºC. 12 After cooling, the resulting solution was filtered through Whatman GF/A glass fibre paper and then 13 freeze-dried to recover the collagen. A sub-sample (c. 0.5-1 mg) of the dried collagen was 14 transferred into tin capsules for measurement of elemental abundance and stable isotope ratios. 15 Sample elemental abundances of %C and %N, to calculate CN ratios, were measured using a 16 Costech elemental analyser (EA) (Milan, Italy) and fitted with a zero-blank auto-sampler. 17 Vegetation samples were measured at the University of St. Andrews Facility for Earth and 18 Environmental Analysis and bone collagen samples were measured at the Scottish Universities 19 Environmental Research Centre. The sample CN ratio was used to screen collagen samples for 20 purity; samples with ratios of 2.9-3.6 were included in the dataset (c.f. DeNiro 1985). Following 21 combustion in the EA, the δ13C and δ15N of vegetation samples was measured using a 22 ThermoFinnegan Deltaplus XL and the δ13C and δ15N of collagen was measured using a Thermo 23 Fisher Scientific Delta V Advantage isotope ratio mass spectrometer (IRMS) (Thermo 24 FisherScientific Inc. GmbH, Bremen, FRG). The EA and IRMS were linked via a ConFlo III 25 (Werner et al. 1999). Isotope values thus obtained are reported as per mil (‰) deviations from the 26 VPDB and AIR international standards for δ13C and δ15N. Samples were measured with a mix of 27 appropriate laboratory standards and blanks, from which measurement precision (1σ) for δ13C was 28 determined to be better than ± 0.2‰ and measurement precision (1σ) for δ15N was better than ± 29 0.3‰. Statistical differences in isotope values between archaeological sites for each archaeofaunal 30 species were assessed using one-way analysis of variance (ANOVA) and post hoc Tukey tests. 31 32 2. Results and Interpretations 33 34 2.1. Modern vegetation and biota from Mývatnssveit 1 2 2.1.1. Modern terrestrial vegetation 3 The raw δ13C values of modern terrestrial vegetation were adjusted by +1.57‰ (Feng and 4 Epstein 1995, McCarroll and Loader 2004, McCarroll et al. 2009) to account for the decrease in 5 atmospheric δ13C since c. AD 1880 due to human burning of fossil fuels (the Suess effect (Keeling, 6 1979; Keeling et al., 1979)). The corrected δ13C values ranged from −30.0 to −25.3‰ and the δ15N 7 values ranged from −9.0 to +6.5‰ (Table 8.2; Fig. 8.2). These values accord with previous 8 measurements by Wang and Wooller (2006) and Gratton et al. (2008) of plant δ15N values for a 9 range of locations in Iceland. The δ13C values of all sites falls within the same broad range. In 10 contrast, the δ15N values of samples from Haganes, Kálfaströnd and Hrúteyjarnes (+0.4 to +6.5‰; 11 average: +2.9‰) is higher than that of samples from Framengjar, Sveigakot and Seljahjallagil (−9.0 12 to +1.1‰; average: −3.7‰). The sampling sites of Hrúteyjarnes and Framengjar in particular were 13 selected due to the lack of modern grazing animals at these locations, meaning that the elevated 14 δ15N values at Hrúteyjarnes are unlikely to be due to the effect of manuring via these species. An 15 alternative explanation for higher plant δ15N values at Haganes, Kálfaströnd and Hrúteyjarnes is 16 higher δ15N of bioavailable soil nitrogen (as NH + or NO -) at these sites. One potential source of 4 3 17 this is the transportation of nitrogen from the lake to the shore in the bodies of chironomids (non- 18 biting midges). Gratton et al. (2008) estimated that, on average, 17 kg N ha-1 d-1 (kilograms of 19 nitrogen per hectare, per day) were transported from Lake Mývatn to the terrestrial environment in 20 this way and that midge abundances decreased logarithmically with distance from shore. In contrast 21 to our results, Gratton et al. (2008) did not find elevated δ15N values in plants close to Mývatn. A 22 further potential source of elevated plant δ15N values close to the lake is that of guano from nesting 23 bird populations. Bird guano has been shown to elevate plant δ15N values considerably in 24 experimental studies (Szpak et al. 2012a). 25 The results of stable isotope measurements on modern vegetation show that there is a wide 26 range in δ13C and δ15N values in plants in the Mývatn area. While δ13C is variable at all sites, δ15N 27 values appear to differ significantly between locations. The expected δ15N values of modern 28 herbivores consuming plants exclusively from Framengjar, Sveigakot and Seljahjallagil would 29 therefore be ~0-2‰, whereas the expected δ15N values of animals consuming plants at Haganes, 30 Kálfaströnd and Hrúteyjarnes would be ~6-8‰. These values are based on the average δ15N value 31 of plants at these locations, meaning that the actual range in animal δ15N values at any location is 32 likely to be larger than the values quoted above. Despite this, the overall δ15N value of a population 33 at Hrúteyjarnes, for example, would be expected to be higher than an equivalent population at 34 Framengjar. 1 2 2.2. Modern freshwater biota and birds 3 The range in δ13C and δ15N values within modern freshwater biota in Mývatn, with respect to 4 internal spatial lake variability, is discussed in detail in Ascough et al. (2011). However, the overall 5 δ13C and δ15N values of lake biota also have relevance for the isotope values of wild resources 6 (freshwater fish and birds) that were exploited by the Norse inhabitants of Mývatnssveit. The range 7 in isotope values for individual species fits the established food web of Mývatn presented in 8 Einarsson et al. 2004, where the trophic pathways from detritus up to waterfowl and fish are 9 illustrated. The overall δ13C value of modern freshwater biota is higher than that of terrestrial 10 plants, meaning that the δ13C values of fish and birds obtaining carbon from the lake will generally 11 be higher than that of terrestrial herbivores (cf. Ascough et al. 2012). In contrast, the δ15N values of 12 aquatic plants and invertebrates are within the range of that represented in terrestrial vegetation 13 samples. Excluding an extreme δ15N value of −16‰ (discussed in Ascough et al. 2011), the δ15N 14 value range is −4.3 to +6.1‰. This means that the δ15N of organisms consuming freshwater 15 resources will overlap with that of organisms consuming terrestrial plants in Mývatnssveit 16 (Ascough et al. 2012). An important point concerning the δ13C and δ15N values of modern 17 freshwater biota is that values for both these isotopes show large variability within the lake. This 18 variation may therefore be reflected in the δ13C and δ15N values of organisms consuming lake biota. 19 The δ13C values of modern bird bones from around Mývatn ranged from −23.2 to −7.9‰ 20 and the δ15N values for these samples ranged from +1.3 to +16.4‰ (Table 8.2, Fig. 8.6). The very 21 wide range in these values reflects the broad diet of the sampled birds. While some species have a 22 diet of terrestrial material (e.g., the whimbrel), the majority of other species incorporate freshwater 23 and marine resources in their diets. The broad range in freshwater biota δ13C and δ15N values 24 discussed above is hence represented in the δ13C and δ15N values of bird tissues. In addition, some 25 birds represented in the sample group are piscivorous (Slavonian grebe), hence will be at higher 26 trophic levels than other species. In addition, most are migratory, spending part of the year in 27 marine environments. This means that the δ13C and δ15N values of their tissues represent an 28 integration of many different dietary resources from a variety of locations. One important point here 29 regards differences in tissue turnover rates; the isotopic values of tissues with rapid turnover (e.g. 30 muscle) reflect recent diet, whereas tissues with slower turnover (e.g. bone collagen) reflect longer- 31 term dietary averages (Hobson and Clark 1992). Therefore, the bone collagen δ13C and δ15N values 32 of migratory birds measured in this study may not exactly reflect the values of the tissues consumed 33 by humans exploiting these birds as a dietary resource, a factor that should be considered before 34 applying these data within the context of a palaeodietary baseline.