We are IntechOpen, the world’s leading publisher of Open Access books Built by scientists, for scientists 6,100 167,000 185M Open access books available International authors and editors Downloads Our authors are among the 154 TOP 1% 12.2% Countries delivered to most cited scientists Contributors from top 500 universities Selection of our books indexed in the Book Citation Index in Web of Science™ Core Collection (BKCI) Interested in publishing with us? Contact [email protected] Numbers displayed above are based on latest data collected. For more information visit www.intechopen.com Chapter 9 Antioxidant Properties of Selected African Vegetables, Fruits and Mushrooms: A Review R.U. Hamzah, A.A. Jigam, H.A. Makun and E.C. Egwim Additional information is available at the end of the chapter http://dx.doi.org/10.5772/52771 1. Introduction Africa is blessed with vast amount of vegetables, fruits and mushrooms which are consumed for their nutrients or for their medicinal purposes. In recent years these vegetables, fruits and mushrooms have been shown to possess valuable antioxidants of great nutritional and therapeutic values. Antioxidants are substances which when present at low concentration compared to those of an oxidizable substrate [1] significantly delay or prevent the oxidation of that substrate. They are capable of preventing or attenuating damages such as lipid peroxidation, oxidative damage to membranes, glycation of proteins and inactivation of enzymes caused by free radicals. There are several evidences that show that oxidative stress resulting from reactive oxygen species including free radicals such as hydroxyl (OH.), superoxide (O .-), nitric oxide (NO.), nitrogen dioxide (NO .-), peroxyl 2 2 (ROO.) and non free radical like hydrogen peroxide and singlet oxygen play an important role in the development of several pathological conditions such as lipid peroxidation, protein oxidation, DNA damage and cellular degeneration. These have been implicated in the aetiology of these pathological conditions related to cardiovascular diseases, diabetes, inflammatory diseases, cancer, Alzheimer and Parkinson disease, monogolism, ageing process and perhaps dementia [2,3-4, 5] . Free radicals and other reactive oxygen species are constantly formed in the human body during normal cellular metabolism e. g during energy production in the mitochondria electron transport chain, phagocytosis, arachidonic acid metabolism, ovulation, fertilization and in xenobiotic metabolism [6]. They can also be produced from external sources such as food, drugs, smokes and other pollution from the environment [7]. Organisms are endowed with endogenous (catalase, superoxide dismutase, glutathione peroxidase/reductase) and exogenous (vitamin C, E, β-carorene) antioxidant defense system against reactions of free radicals. However the generation of free radicals in the body beyond its antioxidant capacity leads to oxidative stress which has been implicated in the aetiology of several pathological © 2013 Hamzah et al., licensee InTech. This is an open access chapter distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. 204 Mycotoxin and Food Safety in Developing Countries conditions such as lipid peroxidation, protein oxidation, DNA damage and cellular degeneration related to cardiovascular disease, diabetes, inflammatory disease, cancer and parkinson disease [8]. As a result of this much attention is been focused on the use of antioxidants especially natural antioxidant to inhibit and protect damage due to free radicals and reactive oxygen species. Synthetic antioxidant such as butylated hydroxyanisole(BHA), tert-butylated hydroxyquinone and butylated hydroxytoluene have been of utmost concern to many researcher because of their possible activity as promoters of carcinogenesis[9] Plant based antioxidant are now preferred to the synthetic ones because of their safety. Epidemiological studies have shown that the consumption of vegetables and fruits can protect humans against oxidative damage by inhibiting or quenching free radicals and reactive oxygen species [8].Many plants including fruits and vegetables are recognized as sources of natural antioxidants that can protect against oxidative stress and thus play an important role in the chemoprevention of diseases that have their aetiology and pathophysiology in reactive oxygen species (10, 11-12]. These positive effects are believed to be attributable to the antioxidants; particularly the carotenoids, flavonoids, lycopene, phenolics and β-carotene [13] Mushrooms which have long been appreciated for their flavour and texture are now recognized as a nutritious food as well as an important source of biologically active compounds of medicinal value [14]. Mushrooms accumulate a variety of secondary metabolites, including phenolic compounds, polyketides, terpenes and steroids. Also, a mushroom phenolic compound has been found to be an excellent antioxidant and synergist that is not mutagenic [15]. Studies have shown that tropical mushrooms are highly rich in proteins, minerals, vitamins, crude fiber and carbohydrate with low fat and oil content. The protein content of mushrooms has been reported to be twice that of vegetables and four times that of oranges and significantly higher than that of wheat [16, 17]. The high level of vitamins in mushrooms particurlary vitamin C and D has been reported as responsible for its antioxidative activity [17, 18]. Mushrooms contains also an appreciable quantities of crude fibres, although, little information exist on Total Dietary Fibre (TDF) content of mushrooms. The crude fibre content values reported from many studies suggest that mushrooms are potential sources of dietary fibre [16]. Mushrooms generally contain low fat and oil content [16]. Because of the low fat and oil content, they are recommended as good source of food supplement for patients with cardiac problems or at risk with lipid induced disorders. Also a lot had been reported on the nutrient; antinutrient and mineral composition of some edible mushrooms in Nigeria [19, 20] however there are few reported data on the antioxidant properties of commonly consumed mushrooms. This Chapter is therefore intended to discuss the antioxidant properties of selected African vegetables fruits and mushrooms. 2 Antioxidant properties of selected vegetables 2.1. Vernonia amygdalina (VA) Vernonia amygdalina is a perennial shrub that belongs to the Asteraceae family and is popularly called bitter leaf in English a. It is known as ‘Grawa’ in Amharic, ‘Ewuro’ in Antioxidant Properties of Selected African Vegetables, Fruits and Mushrooms: A Review 205 Yoruba, ‘Etidot’ in Ibibio, ‘Onugbu’ in Igbo, ‘Ityuna’ in Tiv, ‘Ilo’ in Igala ‘Oriwo’ in Edo and ‘Chusar-doki’ in Hausa.It has petiolate leaves of about 6mm diameter and ellicptic in shape. The leaves are green with a characteristic odour and bitter taste [21]. They are well distributed in tropical African and Asia and are commonly found along drainage lines and in natural forest or commercial plantation. In most part of Africa, the leaves of VA are used as soup condiments after washing or boiled to get rid of the bitter taste. Specifically it is used to prepare the popular Nigerian bitter leaf soup, “onugbo” and as spice in the Cameroon dish called “Ndole” [22]. VA has a long history of use in folk medicine particularly among the sub Saharan African. Huffman and Seifu [23] reported the use of VA in the treatment of parasite related disease in wild chimpanzee in Tanzania. This necessitated quite a great number of researches to test the efficacy of different part of the plant in managing a wide array of ailments [22, 24]. Many traditional medicine practitioners use different parts of the plants in treating various ailments for instance the whole plant is being used as antihelminth, antimalaria and as a laxative [25]. Others use the aqueous extract of the leaves as a digestive tonic, appetizer and for treatment of wounds [26]. The decotion from leaves is used in the treatment of malaria fever in Guinea and cough in Ghana [24]. The leaf is also in Ethiopia as hops in preparing beer [27]. In Malawi and Uganda it is used by traditional birth attendants to aid expulsion of placenta after birth, aid post-pertum uterine contraction, induce lactation and control postpartum haemorrhage. Their traditional use is not limited to human alone, in northern Nigeria it has been added to horse feed to provide a strengthening or fattening tonic chusan Dokin in Hausa. Different extracts of VA has been shown to posses antioxidant properties both invitro and invivo. Ayoola et al [28] showed the invitro antioxidant properties of the ethanolic extract of leaves of VA using the diphenyl picyryl hydrazyl radical (DPHH) scavenging test. V. amygdalina was shown to have moderate inhibition of 77.7% thus indicating the scavenging ability of the vegetable. Also the aqueous and ethanolic extract of VA has further been shown to have potent antioxidant properties as they were able to inhibit bleaching of B- carotene, oxidation of linoleic acid and lipid peroxidation induced by Fe2+/ ascorbate in a rat liver microsomal preparation. This study showed that the antioxidant activity of the ethanolic extracts was higher than that of the aqueous extracts, and compared favourably with synthetic antioxidant BHT and BHA [29]. However another study reported that methanol extract displayed highest antioxidant activity followed by acetone and water extract [30]. Adesanoye and Farombi [31] reported the hepatoprotective activities of the aqueous extract of Vernonia amygdalina leaves against carbon tetrachloride-induced hepatotoxicity and oxidative stress in mice. Administration of Vernonia amygdalina resulted in accelerated reversion of hepatic damage via reduction of liver marker enzymes like ALT, AST, ALP, Lactate dehydrogenase and bilirubin. Similarly antioxidant enzymes such as superoxide dismutase, glutathione S-transferase and reduced glutathione concentration and catalase activity were increased significantly at different doses of the methanolic extract of VA. This 206 Mycotoxin and Food Safety in Developing Countries study is in agreement on previous work reported on the antioxidant properties of VA on aacetaminophen induced hepatotoxicity in mice [32]. The presence of flavonoids, phenols and other phytochemicals in this vegetable have been attributed to its antioxidant properties Further confirmation of the antioxidant activities of VA was carried out by Oloyede and Ayila [33]. They investigated the antioxidant activity of different extracts, aqueous, methanol, hexane, ethylacetate and butanol extracts of Vernonia amygdalina using three methods: scavenging effect on 2,2-diphenyl-1-picryhydrazyl radical (DPPH), hydroxyl radical and peroxide oxidation by ferric thiocynate method. All fractions showed significant antioxidant activity (p<0.05) when compared with antioxidant standards like butylated hydroxyl anisole (BHA), ascorbic acid and α-tocopherol used in the assay. This plant contains natural antioxidants against aqueous radicals and reactive species ions [30]. Oxidative stress has been implicated in numerous human diseases including cancer, atherosclerosis, diabetes, malaria, iron overload, rheumatoid arthritis, Parkinson disease, and in HIV infection and AIDS [1]. This term actually refer to the imbalance between the generation of reactive oxygen species and the activity of the antioxidant defenses[34].Reactive oxygen comprises both free radicals such as hydroxyl (OH!), superoxide (O .-), nitric oxide (NO. ), nitrogen dioxide (NO .-), peroxyl (ROO.) and lipid 2 2 peroxyl (LOO. And non free radical or oxidants like hydrogen peroxide (H O ), ozone (O ), 2 2 3 singlet oxygen ( O.), hypochlorous acid (HOCl), nitrous acid (HNO ), peroxynitrite 1 3 (ONOO¯), dinitrogen trioxide (N O ), lipid peroxide (LOOH), oxidants, although, they can 2 3 easily lead to free radical reactions in living organisms [35]. Many of these ROS serve useful physiological functions but can be toxic when generated in excess or inappropriate environment thus causing oxidative damage to membranes and enhanced susceptibility to lipid peroxidation or enzyme inactivation. Vernonia amygdalina has been used in various part of Africa for the treatment of several ailments ranging from diabetes, malaria, cancer and for general wellbeing. This local treatment has been backed up in recent times scientifically. Nwanjo [36] reported the antidiabetic effect of the aqueous extract VA in streptozotocin induced diabetic rats. He showed in his finding that VA was capable of reducing plasma glucose, triglycerides, and LDL-cholesterol and the marker of oxidative stress malondialdehyde. These may be due to decreased oxidative stress which may be via direct scavenging of the ROS or by increasing the synthesis of antioxidant molecule [37]. Recently Akpaso et al [21] showed that the antidiabetic effect of the combined leaf extracts of vernonia amygdalina (bitter leaf) and Gongronema latifolium on the pancreatic β – cells of streptozotocin – induced diabetic rats. The extracts were observed to increase the animal body weight against the loss in weight in the diabetic group. In the same manner the serum glucose significantly decreased after 28days of treatment with the combined extract. Regeneration of islets cells was explained to be the one of the possible cause as there will be an increase in insulin production and secretion [38]. Previous studies by Ebong et al [39] reported this possible synergestic action using the extracts of Azadirachta indica and VA. It has been clearly demonstrated that Vernonia amygdalina extract contains active ingredients Antioxidant Properties of Selected African Vegetables, Fruits and Mushrooms: A Review 207 such as vernoniosides, glucosides, (VA) flavonoids and antioxidants [40] which may be responsible for their potentials in reversing pancreatic damage caused by STZ or alloxan in experimental animals. It was proposed that sesquiterpene lactones and the bitter principle of the plant may also be responsible for insulin production, stimulation and release of pancreatic islets from the beta-cells [41]. On the other hand, tannin, flavoniods glycosides and phytosterols of the plant may also act as alpha glucosidase inhibitor which contributed to the hypoglycemic effect of the plant. Cancer has become a serious global problem. Prostate cancer and breast cancer are the most diagnosed non-skin cancers in men and women respectively. Breast cancer represents 15% of new cases of all cancers [42] while prostate cancer represents 15.3% of all cancers in men in the developed countries [43]. V. amygdalina Del. is increasingly becoming a strong contender for cancer management. Coumarins, flavonoids, sesquiterpene lactones and edotides may be the principles in VA that are responsible for its anticancer activity [44-46]. It was reported that the aqueous extract of VA exhibited a cytotastic action on cultured human breast tumour cells (MCF-7) growth in vitro. This implies tumour stabilization or preventive effects in vivo [46]. Fractions of Vernonia amygdalina extract were found to inhibit DNA synthesis. However the physiological concentrations of the water-soluble Vernonia amygdalina extract potently inhibited DNA synthesis in a concentration-dependent manner both in the presence and absence of serum [27]. It was also reported that fractions of hexane, chlorofrm, butanol and ethylacetate extracts of VA was capable of inhibiting the growth of human breast cancer cells even at very low concentrations of 0.1 mg/ml to concentration of 1 mg/ml, the inhibition was as high as 98% for some fractions of the extract [47] Cold water, hot water and ethanol extract were found to induce apoptosis against acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML) from the patients with IC50 ranging between 5 to 10 μg/ml. Ethanol extract was found to be most effective against both ALL and AML when compared to cold and hot water extract [48. Petroleum ether/ ethyl acetate leaf extract also possessed cytotoxic effect towards human hepatoblastoma (HepG2) and urinary bladder carcinoma (ECV-304) cell lines [49]. These findings establish the usefulness of V. amygdalina Del. in managing breast cancer. Bioactive peptides from the aqueous extract of the plant leaves (edotides) have been shown to be potent in managing cancer by its activity on mitogen activated protein kinases and signal transduction pathways [46, 50]. Vernoninia amgdalina leaf is a vegetable with several potentials in the prevention and treatment of various ailments associated with oxidative stress. 2.2. Telfairia occidentalis (T.O.) Telfairia occidentalis Hook f. commonly called fluted pumpkin occurs in the forest zone of West and Central Africa, most frequently in Benin, Nigeria and Cameroon. It is a popular vegetable all over Nigeria. It has been suggested that it originated in south-east Nigeria and was distributed by the Igbos, who have cultivated this crop since time immemorial [51]. It is 208 Mycotoxin and Food Safety in Developing Countries a vigorous perennial vine, growing to 10m or more in length. The stems have branching tendrils and the leaves are divided into 3– 5 leaflets. The fruits are pale green, 3 – 10 kg in weight, strongly ribbed at maturity and up to 25cm in diameter. The seeds are 3– 5cm in diameter [52]. The leaf is consumed in different parts of the country because of the numerous nutritional and medicinal attributes ascribed to it. It has different traditional names; among Igbos, it is known as “Ugu”, “iroko” or aporoko in Yoruba, ubong in Efik, umee in Urhobo and umeke in Edo [53]. Young succulent shoots and leaves are used as vegetables in the eastern part of Nigeria. The herbal preparation of the plant has been employed in the treatment of sudden attack of convulsion, gastrointestinal disorders, rmalaria and anaemia [54].Also the plant has agricultural and industrial importance in addition to its nutritional value [55]. Quite a number of researchers in the field of medical sciences have observed free radical scavenging ability and antioxidant property in Telfairia occidentalis. The darkish green leafy vegetable of Telfairia occidentalis and extracts (such as aqueous and ethanol extracts) from the leaves have been found to suppress or prevent the production of free radical and scavenge already produced free radical, lower lipid peroxidation status and elevates antioxidant enzymes (such as superoxide dismutase and Catalase) both in vitro and in vivo ([56,57- 61,62]. They reported that extracts of this vegetable using various solvents were able to offer a chemopreventive and protective effects on oxidative stress induced serum and organs like kidney, liver and brain. Studies have shown that T.O. leaves are rich in antioxidants such as ascorbic acid and phenols [63, 64]. Specifically Oboh et al [57] in their study showed the antioxidant properties of T. O. by assessing their total phenolic content, reducing property and free radical scavenging properties against DPHH radical. From that study the aqueous extracts had a significantly higher total phenol content than the ethanolic extracts which clearly indicates that the phenols present in Telfairia occidentalis leaves are more water soluble than ethanol, consequently, the aqueous extracts could be a more potent antioxidant than the ethanolic extracts. this gives credit to the fact that aqueous extracts of the leaf is presently used in the management and prevention of anaemia and diabetes. This high phenol content in the aqueous extracts could have contributed to the prevention/ management of hemolytic anaemia [65] diabetes [66] which is associated with free radical damage. Antioxidants may been classified into two separate groups: those that suppress the generation of reactive oxygen species and those that scavenge the reactive oxygen species generated[57] . Also in the same study it was observed that the aqueous extract had a significantly higher reducing power and higher free radical scavenging ability than the ethanolic extracts. The higher phenolic content in the aqueous extract would have accounted for the higher ability of the aqueous extract to reduce Fe (III) to Fe (II) in the FRAP test for reducing ability [67]. Also the chelating properties of phenols have been reported to have high reducing power [68] which clearly indicate that Telfairia occidentalis leaf antioxidant potentials will be more harness in its aqueous extraction than the ethanolic extraction and this is in accord with the form in which the plant is presently been used. Furthermore, the high reducing power and free radical scavenging ability of the extracts clearly indicate that Antioxidant Properties of Selected African Vegetables, Fruits and Mushrooms: A Review 209 both extracts of Telfairia occidentalis could suppress the generation of free radical and scavenge free radical. Protocatechiuc acid (PRA) and caffeic acid was shown to be the main polyphenolic compound present in the leaves of T.O.[69]. Cafeic acid is a phenollic compound present in the plant kingdom [70]. It is known to have a large number of physiological activities including anti-inflammatory, anti allergic and anti tumour [71, 72, 73]. They also revealed in their study the high flavonoid content, total antioxidant content, lipid peroxidation inhibition, free scavenging activity towards hydroxyl radical and superoxide scavenging abilities of Telfairia occidentalis amongst other vegetables. Therefore the consumption of leaves of T O will provide adequate antioxidants capable of preventing diseases arising from oxidative stress thus promoting the general well being of an individual. The hepatoprotective properties of polyphenol extracts on T O leaves on acetaminophen induced liver damage was observed [58]. It was demonstrated that the soluble free polyphenol had a higher protective effect on the liver than bound polyphenol in this vegetable. This agrees with previous studies where correlation was reported between antioxidant properties and total polyphenolic content of some commonly consumed vegetables and fruits [56, 57, 74, 75,] Free phenolics are more readily absorbed and thus exert beneficial bioactivities in early digestion. The significance of bound phytochemicals to human health is however not clear [75, 76] and Chu et al 2002. Telfairia occidentalis leaves have been reported to also be protective against liver damage [76, 77]. The use of the leaves in folk medicine in Nigeria in the treatment of certain diseases in which the participation of reactive oxygen species have been implicated could be as a result of the antioxidant and free radical scavenging ability [62]. Oxidative stress which have been implicated in quite a number of diseases such as anaemia, malaria, diabetes cancer and so on have been reported to be relieved by antioxidants inherent in vegetables, fruits and other plants. It is to this end that Salama et al [78] reported that aqueous extract of Telfairia occidentalis leaves reduces blood sugar and increases haematological and reproductive indices in male rats. T. occidentalis actually caused significant increases in packed cell volume, haemoglobin concentration, red blood cell count and white blood cell count in addition to a significant decrease in blood glucose. The increase in the hematological indices observed in this study is consistent with the observations made when rats were fed with the diet preparation of the air-dried leaves of T. occidentalis for four weeks [79] This study has also shown for the first time that new blood cells would have started appearing in the circulation after the fifth day of treatment with T. occidentalis and the increase would become significant after the seventh day of treatment and beyond. This increase is due to the chemical composition of Telfairia occidentalis particularly the presence of the vitamin A and C which are well known antioxidants capable of scavenging free radicals [80]. Some of these constituents are well-established haemopoietic factors that have direct influence on the production of blood in the bone marrow. For instance, iron is a well known haemopoietic factor [81]. Also the amino acids derived from T. occidentalis could also be used for the synthesis of the globin chains of the haemoglobin and this could also contribute to the increase in haemoglobin concentration. The significant increase observed in this study is however inconsistent with the insignificant change in haematological parameters observed when birds 210 Mycotoxin and Food Safety in Developing Countries were fed with the dietary preparation of the sun-dried leaves of the plant [82]. The insignificant change observed with the sun-dried leaves might be due to the denaturing of the active ingredients especially proteins in the leaves during exposure to sunlight. In addition, the inconsistence may be an indication of a species variation in the responses to the effects of the plant. In the same study the leaves were observed to reduce blood sugar significantly, an indication of its hypoglycemic properties. This was confirmed in recent study on the comparative hypoglycemic properties of the ethanolic and aqueous extracts of leaves and seeds of this plant [83]. The hypoglycemic property is more in the leaves and was concluded to be better extracted with ethanol than water. In the same way it was shown that this leave extract improve sperm motility, viability and counts generally improving sperm quality [78]. This is attributed to the actions of some of its active ingredients which have well documented spermatogenic activities. In this respect, studies have shown that nutritional therapies with zinc [84], vitamin C [85], vitamin E [86] and arginine [87] proved beneficial in treating male infertility. Therefore it may be very useful in the treatment and management of infertility especially that associated with reduction in sperm performance. The antianaemic potentials of the aqueous extract of leaves of Telfairia occidentalis extracts against phenyl hydrazine-induced anaemia in rabbits was investigated [88]. Anaemia constitutes a serious health problem in many tropical countries because of the prevalence of malaria and other parasitic infections. In anaemia there is decreased level of circulating haemoglobin, less than 13 g dL-1 in male and 12 g dL-1 in females [89]. In the tropics, where malaria is endemic, between 10 to 20% of the population presents less than 10 g dL-1 of Haemoglobin [90]. Children are more vulnerable. The leaves are rich in iron and play a key role in the cure of anaemia, they are also noted for lactating properties and are in high demand for nursing mothers [91]. Elaboration of the therapeutic effect of Telfairia occidentalis on protein energy malnutrition- Induced liver damage was specifically emphasized in previous study [61]. The protein deficient diet caused a significant increase in hepatic malondialdehyde (MDA) level and the liver function enzymes alkaline phosphatase (ALP), alanine amino transferase (ALT) and aspartate amino transferase (AST) activities in the serum. It also caused a marked reduction in glutathione level, significant decrease in the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) and significant damage to the hepatocytes. Recovery diets of protein alone and protein supplemented with T. occidentalis had significant effects on all the parameters. The MDA level and the serum liver function enzymes were significantly reduced while glutathione and antioxidant enzymes levels were markedly increased and a highly significant hepatocyte healing observed in the histology images. 2.3. OCIMUM The genus ocimum is represented by over 50 species of herbs and shrubs in Africa. Ocimum basilicum and Ocimum gratissimum are known in Africa to manage different diseases. They belong to the family of plant known as Lamiaceae [92]. Local names of different species of Antioxidant Properties of Selected African Vegetables, Fruits and Mushrooms: A Review 211 ocimum in various ethnic groups include Efirin (Yoruba), neh-anwu (Ibo), ntion (Efik) and dai-doya ta gida (Hausa). The leaves can be petiolate or sessile, often toothed at the margin. They are erected and have characteristic pleasant aroma due to their volatile oil [92]. Ocimum gratissimum leaf or the whole plant is known to be popular treatment remedy for diarrhoea [93]. The plant is rich in voltile oils, which contain up to 75 percent of thymol, the antimicrobial activity of which is well known. Infact, the antimicrobial activity of the water- saturated oil had been shown to be proportional to the thymol content [94]. Ocimum gratissimum is effective in the management of upper respiratory tract infection, diarrhoea, headache, skin disease, pneumonia, fever, and conjuctivities.[95]. Traditionally Ocimum basilicum (basil) has been used as a medicinal plant for various ailments, such as headaches, coughs, diarrhoea, constipation, warts, worms and kidney malfunction. It is also thought to be an antispasmodic, stomachicum, carminative, antimalarial, febrifuge and stimulant [96, 97]. Ethnobotanical surveys report the traditional utilization of basil as a veterinary medicinal plant as well. Basil oil, especially the camphor containing oil, has antibacterial properties. The vapour of boiling leaves is inhaled for nasal or bron-chial catarrh and colds. The leaves may be rubbed between the palms and sniffed for colds. It cures stomach- ache and constipation. The leaves are crushed and the juice is used as vermifuge. It is further used to repel mosquitoes and as a broom to sweep chicken house in order to get rid of fleas. Reactive oxygen species (ROS) have been implicated in some of the disorders associated with the traditional uses of some vegetables, such as malaria, anaemia, gastrointentional tract disorders, diabetes mellitus and inflammatory injury. Hence this forms the basis for the investigation of the antioxidant properties of some of these vegetables in order to validate the acclaimed traditional use. A comparative study on the antioxidant properties of two Nigerian species of Ocimum showed that the methanolic extract of Occimum gratissimum posses a higher polyphenolic, flavonoid comoponent and free radical scavenging activities when compared to the methanolic extract of O. basillicum [98]. Thus this may be reason behind wider utilization of O. gratissimum in Nigerian folk medicine than O. basillicum. Basil has been shown to contain flavonoid glycosides (0.6–1.1%) and flavonoid aglycones. A flavone, xanthomicrol (5, 4’-dihydroxy-6, 7, 8-trimethoxyflavone) was isolated from the leaves of a Nigerian O.basilicum [99, 100]. Basil herb (O.basilicum) contains apart from essential oil and flavonoids also tannins and polyphenols (2.2–2.3%)[ 99, 100]. The phytochemical and antioxidant activity of methanolic and aqueous extract of Ocimum gratissimum (OG) were investigated and the results showed the presence of flavonoids, steroids, cardiac glycosides, tannins, phlobatannins in both extract [101]. The methanoilic extract of OG was shown to exhbit a higher DPHH scavenging activity (84.6%) at 250 μg/ml and a reductive potential of 0.77 at 100 μg/ml comparable with those of gallic acid, 91.4% at 250 μg/ml and ascorbic acid, 0.79 at 60 μg/ml as standards for DPPH scavenging activity and reductive potential, respectively. Thus OG - leaf extracts possess antioxidant potential probably because of its phytochemical constituents which has also been reported in other