Thermo Scientific Microbiology Prepared media selection guide For the isolation, identification, differentiation and susceptibility testing of microorganisms Table of Contents Anaerobe Agar 4 Antimicrobial Susceptibility Testing (AST) Agars 6 Bi-plates 9 Blood Agars 14 Brilliance Chromogenic Media (Clinical) 16 Brilliance Chromogenic Media (Food) 21 Diluents, Water & Peptones 23 Dip-Slides 24 General Purpose Media 25 Pharmaceutical Media 29 ReadyBags 32 Water Testing 33 Culture Media bbyy OOrrggaanniissmm TTyyppee Aeromonas 36 LLiisstteerriiaa 5522 Bacillus cereus 366 MMyyccooppllaassmmaa // UUrreeaappllaassmmaa 5522 Bordetella 37 PPaasstteeuurreellllaa 5533 Burkholderia cepaacciiaa 3377 PPsseeuuddoommoonnaass 5533 Campylobacter 388 SSaallmmoonneellllaa 5544 Clostridium specieess 3388 SSttaapphhyyllooccooccccii // SSttrreeppttooccoocccciii 5577 Coliforms / Escherriicchhiiaa ccoolliii 4400 SSttaapphhyyllooccooccccuuss aauurreeuuss 5588 Corynebacteria 411 SSttrreeppttooccooccccuuss aaggaallaaccttiiaaee 5599 Dermatophytes 41 Trichomonas 60 Escherichia coli O157 42 Vibrio 60 Enterobacteriaceae 43 Yeasts & Moulds 61 Enterococci 47 Yersinia 63 Gardnerella 48 Haemophilus & Neisseria 48 Helicobacter pylori 50 Lactobacilli / Bifidobacteria 50 Legionella 51 Bringing more to microbiology Highest levels of quality & consistency Our culture medium expertise and rigorous quality standards have made us a preferred supplier and trusted source of prepared media to laboratories around the world. 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Anaerobe Agar Anaerobes are widely distributed in nature and are the predominant components of bacterial flora of normal human skin and mucous membranes. This section contains a selection of anaerobe media for the recovery of fastidious anaerobic bacteria. Anaerobe Blood Agar Schaedler Anaerobe Agar Schaedler Anaerobe KV (Wilkins Chalgren) with Sheep Blood with Selective Agar with Lysed with Neomycin Haemin and Vitamin K1 Horse Blood A selective medium for non-sporing anaerobes. A nutritious medium for growth and isolation Highly nutritious selective medium for The addition of neomycin inhibits the majority of obligate and facultative anaerobic isolation of anaerobic Gram-negative bacteria of aerobic and facultative bacteria. organisms, such as the fastidious anaerobic especially Bacteroides and Prevotella spp. bacteria Bacteroides, Prevotella and The thioglycollate-free formulation allows the Porphyromonas spp. growth of aerobic and anaerobic organisms. CCCClllloooossssttttrrrriiiidddiiiuumm BBBBaaaacccctttteeeerrroooiiddeess fragilis BBBBaaaacccctttteeeerrrroooiiddeess ffrragilis ppeerrffrriinnggeennss AATTCC® AATTCCCC®® 225522885™ AATTCCCC®® 2255228855™ 13124™ Image shown Image shown Image shown incubated: 36–48 hr. at incubated: 40–48 hr. at incubated: 40–48 hr. at 35–39°C, anaerobic 36 ± 1°C, anaerobic 36 ± 1°C, anaerobic Product Code Format Product Code Format Product Code Format PB0112A 90mm Plates PB5034A 90mm Plates PO5020A 90mm Plates 4 ® Anaerobe Agar Anaerobe Blood Agar (Wilkins Chalgren) Tubes with Nalidixic Acid and Tween Media for the cultivation of aerobic and Product Code Format anaerobic organisms. PB0113A 90mm Plates Schaedler Broth with Haemin and Vitamin K Anaerobe Blood Agar with Neomycin and Aztreonam Product Code Format PB1092A 90mm Plates TV5008D 50x9 mL, Tube with screw cap Anaerobe Recovery and Isolation Agar Thioglycollate Medium (EP / USP) with Horse Blood TV5001D 50x9 mL, Tube with screw cap PB1243A 90mm Plates Anaerobe Recovery and Isolation Agar Bottles with Horse Blood and Neomycin Media for the cultivation of aerobic and PB1244A 90mm Plates anaerobic organisms in the performance of sterility tests. Columbia Blood Agar with Neomycin PB0219A 90mm Plates Thioglycollate Medium (EP / USP) Fastidious Anaerobe Agar (F.A.A.) with Product Code Format Horse Blood 10x100 mL, Vial – Narrow neck BO0368M PB0225A 90mm Plates with septum Fastidious Anaerobe Agar (F.A.A.) with BO0510V 10x100 mL, DIN – Wide neck bottle with septum Horse Blood and Gentamicin 24x15 mL, Vial – Narrow neck PB0252A 90mm Plates BO0368F with septum 10x100 mL, DIN – Wide neck BO0510M Bi-plates bottle with septum The use of bi-plates saves space and reduces 10x100 mL, Sirop – Screw BO0211M the number of anaerobic jar kits required. cap bottle 24x20 mL, Universal – 1 oz. BO0211G Straight walled Schaedler Anaerobe Agar / Schaedler Anaerobe KV Selective Agar Product Code Format PB5204E 90mm Bi-plate 5 Antimicrobial Susceptibility Testing (AST) Agars A broad range of AST media that conform to international standards EUCAST and CLSI as well as national guidelines including BSAC. Mueller Hinton Agar Mueller Hinton Agar with Mueller Hinton Agar with Horse Blood and NAD Sheep Blood A medium that may be used in internationally recognised standard procedures. Used for testing An antimicrobial susceptibility testing medium A medium for antimicrobial susceptibility testing susceptibility of non-fastidious organisms, and according to EUCAST. The addition of horse blood for non-fastidious organisms. Conforms to CLSI conforms to EUCAST and CLSI. and NAD enables the growth of non-fastidious and DIN. The formulation of Mueller Hinton Agar organisms and the formulation ensures that toxic ensures that toxic factors found during growth will factors will be absorbed to establish better growth be absorbed and its presence is often essential to from very small inocula. establish growth from very small inocula. EEscherichia coli Streptococcus Staphylococcus AAAATCC® 35218™ pneumoniae aureus ATCC® ATCC® 49619™ 25923™ SStaphylococcus Haemophilus Enterococcus aaureus ATCC® influenzae NCTC faecalis ATCC® 225923™ 8468 29212™ IImage shown incubated: Image shown incubated: Image shown incubated: 118 ± 2 hr. at 36 ± 1°C, 18 ± 2 hr. at 36 ± 1°C, 18–24 hr. at 36 ± 1°C, aerobic capnophile aerobic Product Code Format Product Code Format Product Code Format PO5007A** 90mm Plates PB5303A** 90mm Plates PB5007A** 90mm Plates PO0152A* 90mm Plates PB1229A* 90mm Plates PB0431A* 90mm Plates PO1191S 140mm Plates * UK ** Mainland Europe 6 Product codes are valid for all countries, except USA, unless ® stated otherwise. Antimicrobial Susceptibility Testing (AST) Agars Iso-Sensitest Agar Iso-Sensitest Agar with Horse Blood and 20mg/L NAD A closely defined medium with a stabilised mineral content for antimicrobial susceptibility For testing susceptibility of fastidious organisms testing of fastidious organisms that produce according to BSAC. The addition of 5% of optimum non-antimicrobial zones of inhibition. horse blood has been recommended as a Conforms to BSAC. suitable medium when testing susceptibility of Helicobacter pylori. SStaphylococcus aaureus ATCC® 225923™ EEsscchheerrriiiccchhhhiiiaaa coli AAAATTTTCCCCCCCC®®® 33552218™ Streptococcus pyogenes ATCC® Staphylococcus 19615™ aureus ATCC® 25923™ Image shown incubated: Image shown incubated: 18–24 hr. at 36 ± 1°C, 18–24 hr. at 35–39°C, aerobic aerobic Product Code Format Product Code Format PO5003A** 90mm Plates PB0378A 90mm Plates PO0144A* 90mm Plates * UK ** Mainland Europe Product codes are valid for all countries, except USA, unless ® 7 stated otherwise. Antimicrobial Susceptibility Testing (AST) Agars Columbia Agar Base with 2% Salt (4mm Depth) Iso-Sensitest Agar (4mm Depth) Tubes Product Code Format Product Code Format Liquid media in tubes for agar dilution methods. PO0879A 90mm Plates PO0779A 90mm Plates Diagnostic Sensitivity Testing (D.S.T.) Agar Iso-Sensitest Agar with Horse Blood Iso-Sensitest Broth Product Code Format PO5068A** 90mm Plates PB0146A 90mm Plates 50x10 mL, Tube with PO0130A* 90mm Plates Iso-Sensitest Agar with Lysed Horse Blood TV5061E screw cap Diagnostic Sensitivity Testing (D.S.T.) Agar PB0145A 90mm Plates Maximum Recovery Diluent with Sheep Blood Iso-Sensitest Agar with Sheep Blood 50x9 mL, Tube with PB5068A 90mm Plates TV5016D screw cap PB5003A 90mm Plates Haemophilus Testing Medium (HTM) Iso-Sensitest Chocolate Agar PO5138A 90mm Plates Bottles PB0147A 90mm Plates Liquid media in bottles for AST testing. Mueller Hinton Agar with 2% Sodium Chloride 0.9% Saline PO5139A** 90mm Plates Product Code Format PO0799A* 90mm Plates 24x3 mL, Bijou – 1/4 oz. BO0334B Straight walled 200x3 mL, Bijou – 1/4 oz. EB0334B Straight walled 300x3 mL, Tube with BO1176B screw cap 24x5 mL, Bijou – 1/4 oz. BO0334C Straight walled 24x10 mL, Universal – 1oz. BO0334E Straight walled 10x100 mL, Sirop – BO0334M screw cap bottle 10x1000 mL, Sirop – BO0334W screw cap bottle * UK ** Mainland Europe 8 Product codes are valid for all countries, except USA, unless stated otherwise.