PURDUE UNIVERSITY THIS IS TO CERTIFY THAT THE THESIS PREPARED UNDER MY SUPERVISION Enos G. Pray by An Investigation of the Effects of Various Bacteria en titled and Their Metabolites on the Flagellate Protozoan Trichomonas vaginalis ,___________________ COMPLIES WITH THE UNIVERSITY REGULATIONS ON GRADUATION THESES AND IS APPROVED BY ME AS FULFILLING THIS PART OF THE REQUIREMENTS FOR THE DEGREE OF DOCtOP O f P h ilo so p h y Professor in Charge of Thesis cP'. Head or School or Department r July 27 1*51 TO THE LIBRARIAN:---- XX THIS THESIS IS NOT TO BE REGARDED AS CONFIDENTIAL. GRA»4 SCHOOL FORM » INVESTIGATION OF THE EFFECTS OF VARIOUS BACTERIA AND THEIR METABOLITES ON THE FLAGELLATE PROTOZOAN TRICHOMONAS VAGINALIS A Thesis Submitted to the Faculty of Purdue University by Enos G. Pray In P artial Fulfillm ent of the Requirements for the Degree of Doctor of Philosophy August, 1951 ProQuest Number: 27716041 All rights reserved INFORMATION TO ALL USERS The quality of this reproduction is dependent upon the quality of the copy submitted. In the unlikely event that the author did not send a complete manuscript and there are missing pages, these will be noted. Also, if material had to be removed, a note will indicate the deletion. uest ProQuest 27716041 Published by ProQuest LLC (2019). Copyright of the Dissertation is held by the Author. All rights reserved. This work is protected against unauthorized copying under Title 17, United States Code Microform Edition © ProQuest LLC. ProQuest LLC. 789 East Eisenhower Parkway P.O. Box 1346 Ann Arbor, Ml 48106 - 1346 ACKNOWLEDGEMENTS This investigation was made possible by the grant of an XR Fellowship from the Burdue Research Foundation and was directed by Professor R. M. Cable, Department of Biological Sciences* The author wishes to express his deep appreciation of the help and encouragement given him by Professor Cable throughout the study. Thanks are due also to Mr. M. C. McGowan, of the Eli Lilly Research Laboratories, Indianapolis, for technical advice, and to F * W* Peyton, M* V. who provided flagellate from an acute case of vaginitis at the Women*s Clinic, Lafayette, Indiana* TABLE OF CONTENTS Page ABSTRACT INTRODUCTION AND STATEMENT OF PROBLEM ...................... 1 HISTORICAL REVIEW ................ 4 MATERIALS. AND METHODS ........................................................... 15 Strains of Trichomonas vaginalis ................... 15 Bacterial c u ltu re s ................... 15 Maintenance of Trichomonas vaginalis in stock cultures .............................. 16 Experimental medium....................... 18 Hydrogen ion determinations ......................... 21 Oxidation-reduction potential determ inations................ 21 Preparation of bacterial filtra te s . . . . . . . 23 Determination of flagellate numbers ........... 23 OBSERVATIONS AND DISCUSSION ............................ 25 Normal or Control Growth Curve ............ 25 Phase s of growth ............................... 25 Growth range ........... 27 Effect of Various Bacteria on the Growth of Trichomonas vaginalis . . . . . . . . . . . . . . . 27 Changes in Hydrogen ion Concentration of the Medium ........... 48 Controls .............................. 48 Experimental c u ltu re s .......................... 49 Page Changes in the Oxidation-Reduction P o te n tia l....... ......................................... 50 C o n tro ls.............. ............. 51 Experimental cultures ....................... 52 Effects of B acterial F iltrates ............................. 53 N utritional Studies ...................................................... 56 E« ooli plus enrichment of the medium .... 57 Ps. aeruginosa plus enrichment of the medium ........... 65 A. aerogenes plus enrichment of the medium «................................... 70 SUMMARY. ............................................................................................ 76 APPENDIX. TABLES .................................................................... 78 BIBLIOGRAPHY .......................................................................116 VITA .................................................................................................. 124 LISTS OF FIG-URSS AND TABLES L ist of Figures Figure Page 1, Culture tubes used in oxidation- reduction potential experiments * * ......... 22 2. Normal or control growth curve of T. vaginalis» Oxidation-reduction potential and pH changes caused by T. vaginalis ......................................................... 26 3» Growth of T. vaginalis in the presence of E. co li. Oxidation-reduction poten­ tia l and pH changes caused by E. coli .......... 29 4. Growth of T. vaginalis in the presence of A. aerogrenes. Oxidation-reduction potential and pH changes caused by A. aerogenes .. ................................... 30 5* Growth of T+ vaginalis in the presence of Ps* aeruginosa. Oxidation-reduction potential and pH changes caused by Ps* aeruginosa ........................«............ . 32 6* Growth of T* vaginalis in the presence of S * schottm üllerl. Oxidation-reduction potential and^pH changes caused by S* schottm üllerl .................. 33 7. Growth of T. vaginalis in the presence of P. m irabilis. Oxidation-reduction potential and pH changes caused by P a m irabilis ........... 34 8. Growth of T. vaginalis in the presence of S. paratyphi. Oxidation-reduction potential and pH changes caused by S. paratyphi ........... 35 9* Growth of T. vaginalis in the presence of B* suis. Oxidation-reduction potential and pH changes caused by B. s u i s ................ 37 Figure Page 10, Growth of T, vaginalis in the presence of S, la c tls , Oxidation-reduction potential and pH changes caused by S» lactls ,. ............................................................. ,.. 38 11, Growth of T. vaginalis in the presence of Ps, fluorescens, Oxidation-reduction potential and pH changes caused by Ps, fluorescens ................ 39 12* Growth of T* vaginalis in the presence of A. faecalls. Oxidation-reduction potential and pH changes caused by A » fae calls ................ 40 13, Growth of T. vaginalis in the presence of S, lutea* Oxidation-reduction potential and pH changes caused by . S, lutea ........... 42 14, Growth of T. vaginalis in the presence of B. su b tilis. Oxidation-reduction potential and pH changes caused by B. subtilis ................................................. 43 15, Growth of T. vaginalis in the presence of S. albus * Oxidation-reduction potential and pH changes caused by S. albus ............................ 44 16, Growth of T. vaginalis in the presence of S, aureus, Oxidation-reduction potential and pH changes caused by S. aureus ........... 46 17, Growth of T. vaginalis in the presence of E. coll and A, aerogenes filtra te .......... 54 18, Growth of T. vaginalis in the presence of E, coli and various nutrients ..................... 58 19, Growth of T, vaginalis in the presence of E, coll and various nutrients ............. 59 20, Growth of T. vaginalis in the presence of S. coli andOT2^ maltose ....................................60