BULL. BOT. SURV. INDIA Vol. 47, Nos. 1-4 : pp. 63-86, 2005 ALGAL FLORA OF INDIAN BOTANIC GARDEN, HOWRAH, WEST BENGAL ANKHI SAUA ND R.K. GUPTA Botanical Survey of India, Indian Botanic Garden, Howrah-711103 A B S T R A C T A survey on algal flora of Indian Botanic Garden was undertaken during December 1998 to November 2001. A total of 167 taxa belonging to 79 genera, mainly of Cyanophyceae, Chlorophyceae and Bacillariophyceae have been enumerated for the first time. INTRODUCTION Indian Botanic Garden, Howrah was established in 1787 by Lt. Col. Robert Kyd. It comprises an area of 112 hectares of alluvial soil with 1.5 km river fiont, on the west bank of the river Hoogli. It lies between 22" 35' N, and 88" 21' E. The area experiences annual rainfall of about 1536 mrn and the temperature ranges from 21°C-31°C. There are 24 artificial lakes and ponds of different sizes, in the area, covering a total area of 1-5 acres. These are viz. King's lake, Prain's lake, Sadir lake, Lerum lake and 4 ponds, mainly Lotus pond and Dhobi pond etc. Some of which are connected with underground pipes receiving water from the river Hoogli. The algal flora of West Bengal as a whole, is known by the reports of Prain (1905), Briihl and Biswas (1922, 1923), Biswas (1925, 1926), Banerji (1936, 1938), Caller (1926), Gupta (1965, 1975), Sinha and Mukherjee (1975), Mukhopadhyay and Chatterjee (1981), Pal and Santra (1982), Maity and Santra (1985) and Pal and Santra (1985). But there is no report of the algal flora of Indian Botanic Garden, Howrah. In the present investivation, the algal flora of Indian Botanic Garden, West Ben@ has been studied with a view to find out their distributional pattern under varied ecological conditions. In the paper 39 genera and 70 species of Chlorophyceae; 17 genera and 46 species of Bacillario- phyceae; 3 genera and 10 species of Euglenineae; 17 genera and 38 species of Cyanophyceae and 1 each of Xanthophyceae, Dinophyceae and Rhodophyceae have been enumerated. Date of receipt : 08.02.2003. Date of acceptance : 14.09.2004. BULLETIN OF THE BOTANICAL SURVEY OF INDIA [Vol. 47 MAIERMLS AND METHODS The algal samples were collected seasonally and periodically, at random, during winter (December February), summer (March May), monsoon (June August) and post monsoon (September November) seasons for three years 1998-2001. The samples were also collected from different habitats of 1.B.G like scum of the moist soils, submerged soils, moist walls, bricks, logs, shell of molluscs etc. for which special care was taken. Phytoplankton was collected in the morning from different spots of the lakeslponds with the help of plankton net (mouth area 0.25m2 and mesh size 0.330 rnrn) fitted with wooden stick. Phytoplankton, adhered to the surface of aquatic macrophytes, was collected, after washing the submerged macrophytes with distilled water. These were preserved in 4% aqueous solution of fonnalin, which is added immediately after phytoplankton collection, to prevent zooplanktonic feeding. The filamentous algae (floating, benthic and epiphytic) were preserved in F.A.A. solution. For phytoplanktons, water samples were centrifuged at 1000 rpm, the liquid supernatant discarded and the pellet examined under compound microscope after mounting under 1% glycerine, for identification. Identification of the samples has been carried out on the basis of Biswas (1927); Geitler (1932); Smith (1950); Tiffany & Britton (1952); Desikachary (1959); Randhawa (1959); Venkataraman (1961); Pal & al. (1962); Ramanathan (1964); Prescott (1966, 1982); Philipose (1967); West & West (1978); Foged (1977); Gonzalves (1981); Iyengar & Desikachary (1981); Sarode & Kamat (1984); Sabat & Nayar, (1985, 1992) and other relevant literature. Different classes, order and families are arranged as per the system of classification proposed by Fritsch (1935, 1943, whereas genera and species are arranged alphabetically. Each species has been provided with up to date nomenclature and details of habitat. The number given in the parenthesis indicate the collection number, and the species are arranged alphabetically. The algal materials have been deposited in the Cryptogamic section of Botanical Survey of India, Howrah. CLASS: CHLOROPHYCEAE Order: VOLVOCALES VOLVOCACEAE Vdvox amus Ehr. Prescott, Algae of the Western Great lake area, 78, pl. 2. fig. 4, 1982; Tiffany and Britton, The algae of Ilinois, 18, P1. 2, fig. 21, 1952. Planktonic in Dhobi pond (71 135) and Lotus pond (71 110). Order: CHLOROCOCCALES CHLOR-ACEAE Chlor~~~~hcuwmknd o (Naegeli) Rabenhorst, Philipose, Chlomoccdes, 73 fig. 3. 1967. Free floating in Lotus pond (71110) and King's lake (71128). 20051 SAU AND GUPTA : ALGAL FLORA OF INDIAN BOTANIC GARDEN (W.B.) 65 Hydrodiction reticdatum (Linn.) Lagerheim Philipose, Chlomoccdes, 134, fig. 48. 1967. Free floating in Prain's lake (7 11 06) and King's lake (7 11 28). Pediastrwn simplex Meyen Philipose, Chlorococcales, 113, figs. 36a-c, 1967. Free floating in Dhobi pond (7 11 l o), Lotus pond (7 11 35). P. tetras (Ehr.) Ralfs. Philipose, Chlorococcales, 128, figs. 45a-c, 1967. Planktonic in Prain's lake (71106). Actinastrum hantzschii Lagerheim, Philipose, Chlorococcdes, 217, figs. 125 a-c, 1967. Free floating in Sadir lakes (71 107), Prain's lakes (711106) and Dhobi pond (71135). Ankistrodesmus convolutus Corda, Philipose, Chlomaccale, 213, figs. 122 a-d. 1967. Free floating in Lotus pond (71 110). A. falcatus (Corda) Ralfs. Philipose, Chlorococcdes, 21 1, pl. 121, figs. a, e. 1967. Free floating in Sadir lake (71107), King's lake (71128). Kirchneriella lunaris (Kirchner) Moebius, Philipose, Chlorococcales, 222, fig. 131, 1967. On most soil near Prain's lake (71106)- K. obesa (W. West) Schmidle, Philipose, Chlorococcales, 224, fig. 133, 1967. On moist soil near Prain's lake (71 106). Chlorella vulgaris Beijerinck. Philipose, Chlorocaccales, 173, fig. 82, 1967. Free floating in Dhobi pond (71 135), Sadir lake (71 107). Oocystis elliptica W. West. Philipose, Chlorococcales, 186, fig. 100, 1967. Free floating in King's lake (7 11 28). Scenedesmus acuminates (Lagerheim) Chodat. Philipose, Chlorococcales, 25 1, fig. 161, 1967. Free floating in King's lake (71 128), Lerum lake (71 109), Sadir lake (71 107) and Dhobi pond (71 135). s* obliquus (Turpin) Kuetzing, Philipose, Chlomoccales, 248, fig. 159,1967. Free floating in Prain's lake (7 11 06), Dhobi pond (7 11 35). BULLETIN OF THE BOTANICAL SURVEY OF INDIA Dispora cuneifod (Schrnidle) Printz. Philoipose, Chlorococcales, 193, fig. 107, 1967. Free floating in Dhobi pond (7 11 35). Order: ULOTRICHA~ES ULcillucHAc~~~ Binuclearia tectorum (Kiitz) Beger ex Wichmann, Ramanahan, Ulotrichales, 60, pl. 17 A-V, 1964. Free floating in Lerum lake (71 119). Schizomeris leibleinii Kuetzing, Rarnanathan Ulotrichales, 57, pl. 2, 1964. Free floating in King's lake (71128). Ulothrix zonata Kuetzing Tiffany & Britton, The algae of Illinois, 24, pl. 5, fig. 35, 36, 1952. Free floating in King's lake (71 128) and Prain's lake (711 06). Uronema afficanum Borge. Ramanathan, Ulotrichales, 51, pl. 13, H-L, 1964. Attached on submerged aquatics in King's lake (71108). U. gigas Vischer, Rammathan Ulotrichales, 53, pl. 14 H-U, 1964. Attached on submerged aquatic plants of Sadir Iake (71107). Microsporn amoena (Kiitz.) Raben, Rarnanathan, Ulotrichales, 132, pl. 29, figs, A-C, 2-W, Pl. 30 A, D- F, J. pl. 31, figs. A-D, pl. 37 fig. A-E, 1964. Free floating in Prain's lake (7 11 06). Order: CLADOPHORALES CLADOPHORACEAE Cladophora glomerata (L.) Kuetzing Prescott, Algae of the western great lakes area, 138, pl. 20, figs. 8, 9 pl.. 21, figs 1, 2. 1982. In stagnant water of earthen pots (71 134/255., 249, 2391, Lotus pond (71 110) and Dhobi pond (7 11 3 5). Pitbophora mooreana Collins, Prescott, Algae of the Western great lakes area, 140, pl. 22, figs. 5, 6, 1982. Free floating in Lotus ponds (71 lo), Ohobi pond (71 1351, King's lakes (71 128) and Prain's lake (71 106). 2005 j SAU AND GUPTA : ALGAL FLORA OF INDIAN BOTANIC GARDEN (W.B.) 67 Pithophora oedogonia (Mont) Wittrock, Prescott, Algae of the western great lakes area 140, pl. 22, figs. 7-10, 1982. Free floating in Lerum lake (7 11 1 9) and Lotus ponds (7 11 1 0) forming tangled mats. P, varia Wille, Prescott, Algae of the Western great lakes area, 140, pl. 24, fig. 5-6, 1982. Free floating in King's lake (711 28) and Sadir lake (711 07). Rhizoclonium crassipellitum West & West. Prescott, Algae of the Western great lakes area 141, pl. 23, fig 1. 1982. Free floating in Lerum lake (7 11 1 9) and Dhabi pond (7 11 3 5). R. hieroglyphicurn (Ag.) Kuetzing, Prescott, Algae of the Western great lakes area, 142, pl. 23, fig. 3, 1982. Free floating in Lerun lake (71 119) and Dhobi ponds (225, 245). Order: CHAETOPHORALES CHAJZIUPHORACEAE Drapernaldia glomerata (Vaucher) Ag. Tiffany & Britton, The algae of Illinois, 38, pl. 11 , fig. 80, 1952. Attached on Rhizoclonium sp. in Dhabi pond (7 11 35). Stigedonium subsecundurn Kuetzing Tiffany & Britton, The algae of Rhois, 36, pl. 8, fig. 68, 1952. Attached on submerged logs in Lotus pond (71 110). Coleochaete soluta (Breb) Pringsheim, Tiffany & Britton, The algae of Illinois, 214, pl. 10, fig. 73, 1952. Free floating in Prain;s lake (7 1 106). Order: OEDOC~N~ALES OEDOGONIACEAE Oedogonium angusturn (Him.) Tiffany. Prescott, Algae of the western great lakes area, 165, pl. 29, fig. 1, 2. 1982. Attached on submerged aquatic in Sadir lake (71 107). 0, anomalum Him., Prescott, Algae of the Western great lakes area 166, PI. 29, figs. 3,4, 1982. Attached on submerged aquatic on Sadir lake (71 107). 0. eardiaeum (Hass) Wittrock. Rescott, Algae of the western great lakes area, 168, pl. 29, figs. 7, 8, 1982. Attached with submerged aquatic in King's lake (71128). 6 8 BULLETIN OF THE BOTANICAL SURVEY OF INDIA OedogoUium figaratum Tiff., Gonzalves, Oedogoniales, 323, fig. 9.202, 1981. Free floating in Lerum lake (71 112). 0. gallicum Hirn. Prescott, Algae of the western great lakes of area, 195, pl. 39 fig. 4. 1982. Free floating in Sadir lake (71 lw), Dhobi pond (71 135)' Lotus pond (711 1 0). 0. globosum Nordstedt, Prescott. Algae of Western greallakcs area, 178, pl. 31, fig. 1-3, 1982. Growing on aquatic plants in Prain's lake (711091204, 206). 0. majus (Hansg.) Tiffany. Gonzalves, Oedogoniales, 283, fig. 9. 161. 1981, Prescott, Algae of the western great lakes area, 172. 1982. Free floating in King's lake (7 11 28). 0. rivulare A. Braun, Prescott, Algae of the western great lake areas, 174, pl. 32, fig. 5, 6: 1982. Epiphytic on submerged aquatics of Prain's lake, King's lake (7 11 06111 7, 7 11 28/104). 0, tyrolicum Wittrock., Prescott. Algae of the Western great lakes area, 181, pl. 32, figs. 11, 1982. Epiphytic on submerged aquatic plants in Prain's lake (71 106). 0. varians Wittrock & Lundell. Prescott. Algae of the Western great lakes area 181, pl. 32, fig. 10, 1982. Free floating in Lotus pond (71 10/1 14), Dhobi pond (71 135). Order: CONNGALES ZYGNEMACEAE Sphgyra ellipsospora Transeau, Randhawa, Zygnemaceae 323, fig. 304, 1959. Free floating in Prain's lake (71106), Lenun lake (71119) and Lotus pond (71110). S. gram (Hassall) Kutzing, Randhawa, Zygnemaceae 296, figs. 253a-c, 1959. Free floating in Lotus pond (71 10) and stagnant water of earthen pots (71 1341224). S. maxima (Hassall) Wittrock., Randhawa, Zygnemaceae 348, fig. 356. 1959. Free floating in Dhobi pond (7 11 35), Sadir lake (7 11 07). S. neglects (Hassall) Kutzing. Randhawa, Zygnemaceae 324, fig. 308, 1959. In stagnant water of earthem pots (71 134/201). Zygnem -yc Eleve, Randhawa, Zygnemaceae 225, fig. 159, 1959, Free floating in King's lake (71128), Prain's lake (71 106). 20053 SAU AND GUPTA : ALGAL FLORA OF INDIAN BOTANIC GARDEN (W.B.) 69 - PLATE 1 Figs. 1- 16 : 1. Spirogyra maxim, 2. Ceratium hirundiniella, 3, Clasteriwnp ervd~m4,. Closterium acerosurn, 5. Closterium moniliferum, 6. Micrastehrias meissa; 7. Ulothrix zonata, 8. Tribonema bombycinum, 9. Rivularicr aguatica, 10. Nitzschia amphibia, 1 1. CycEvtellu comta, 12. Pleurosigma angulatum, 13. Gonatozygonp ilosum, 14. Calothrix castethlii, 15. Oscillatoria subbrevis, 16. Closteriurn lanceolatum. BULLETIN OF THE BOTANICAL SURVEY OF INDIA Mougeotia recurva (Hassall) de Toni 1889. Randhawa, Zygnemaceae 127, fig. 17, 1959. Free floating in Dhobi pond (7 11 35) and stagnant water of earthen pot (7 11 34/140, 240). Sirogonium reticulaturn Randhawa, Randhawa, Zygnemaceae, 430, figs. 517a-c, 1959. In stagnant water of earthen pot (711341127) and Dhobi pond (71135). Gonatozygon brebissonii de Bary, Tiffany Bt Britton, The algae of Illinois, 166, pl. 51, fig. 535, 1952. Free floating in Dhobi pond (71135). G. pilosum Wolle, Prescott, Algae of the Panama Canal and its tributaries, 2, 1966. Free floating in King's lake (71128). G. ralfsii de Bary, Prescott, Algae of the panama canal and its tributaries, 2, pl. 11, figs. 11,12,1966. Free floating in Dhobi pond (71 135) and Lotus pond (71 110). Closteriom aceroMtm (Schrank) Ehrenberg, Tiffany & Britton, The algae of Illinois, 169, pl. 52, fig. 550, 1952. Free floating in King's lake (7 1128) and Lotus pond (71 110). C. ehrenbergii Meneghini, Tiffany & Blitton, The algae of Illinois, 172, pl. 52, Fig. 558, 1952. Free floating in Lerum lake (7 11 1 2). C. lanceolatum Kuetzing, Tiffany & Britton, The algae of Illinois, 173, pl. 52, figs. 552, 1952. Free floating in King's lake (71 128), Sadir lake (71 107). C. modliferum (Bory) Ehrenberg, Tiffany & Britton, The algae of Illinois, 172, PI. 52, fig. 549, 1952. Free floating in Prain's lake (71 116), King's lake (71 128) Dhabi pond (71 135) and Lotus panel (71 110). C. pervuh.lm Naegeli Tiffany & Britton, The algae of Illinois, 173, pl. 51, fig. 543, 1952. Free floating in King's lake (71128). cosmarium drculare Reinsch, Prescott, Algae of the Panama canals and its tributaries, 13, pl. 3, figs. 11, 1%6. Free floating in King's lake (71128), Prain's lake (71106) and Dhohi pond (71 135). 20051 SAU AND GUPTA : ALGAL FLORA OF INDIAN BOTANIC GARDEN (W.B.) 7 1 C. connatum W. West, Prescott Algae of the Panama canal and its tributaries, 14, pl. 5, fig. 45, 1966. Free floating in Lotus pond (71 110) and Sadir lake (71 107). C. obtusatum Schmidle, Prescott, Algae or the Panama canal its tributaries, 19, pl. 4, figs. 26, 1966. Free floating in King's lake (71128) and Sadir lake (71107). Euastrum insulare Grijnblad, Prescott, Algae of the hmma canals and its tributaries, 27, pl. 5, fig. 25, 1%. Planktonic in King's lake 711 28, Prain's lake (711 09). Micrasterias meissa Hassal., Prescott, Algae of the Rmarna canal and its Abutaries, 30, pl. 10, fig. 4. 1966. Free floating in Lerurn lake (7 11 1 9) and King's lake (7 11 28). Penium navicula Perty. Prescott, Algae of the Pmn.na canals and its tributaries, 4, pl. 4, fig. 2, 1966. Planktonic in Lotus pond (71 110). Pleurotuenium ehrenbergii (Breb.) De bar^, Prescott, Algae of the Panama canals and its tributaries 9, pl. 12 fig. 1,1966, Planking in Lotus pond (7 11 1 0) and lake (7 11 1 9). Staurastrum dejecturn Nordstedt., Prescott, Algae of the Panama canal and its tributaries, 34, pl. 9, figs. 28, 29, 1966. Free floating in Dhobi pond (7 11 35) and King's lake (7 11 28). Order : SIPHONALES VAUCHENACEAE Dichotomosiphon tuberosus (A. Br.) Ernst., Venkataraman, Vaucheriaceae, 38, figs. 28a-f, 1961. Free floating in Dhobi pond (71 135113 1). Vaucheria sessilis (Vanch) De Condole, Venkataraman, Vaucheriaceae, 68, fig. 469. 1961. On moist soil near Sadir lake (252) Order : CHARALES CHARACEAE Chars brachypus Braun., Pal Kundu Suncialingam & Venkataraman, Charophyta, II1, fig. 259-260, 1962. As benthic in stagnant water of earthen pots (711 341, King's lake (711 28). C* b r a d Gmelin, Pal, Kundu, Sundaralingam &. Venkataraman, Charophyta 89, figs 200-203, 1962. As benthic in Prain's lake (71106) and Dhobi pond (71135). BULLETIN OF THE BOTANICAL SURVEY OF INDIA - PLATE 2 Fig. 7- Figs. 1-9 : 1. Ankistrodesmus convolutus,2 . Cosmarium obtusatum, 3. Phormidium ambiguurn, 4.L yngbya ceylanica, 5. Euglena acus, 6.P hormidium retzii, 7.C hara brachypus, 8. Diatoma vulgare, 9. Closterium ehrenbergii.